This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.41 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 135,674 (6.2%) Reads that were too short: 18,622 (0.8%) Reads written (passing filters): 117,052 (5.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 32,222,570 bp (4.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 135674 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1392 535.2 0 1392 7 42551 535.2 0 42551 8 51973 535.2 0 51973 9 115 535.2 0 115 10 102 535.2 0 102 11 82 535.2 0 82 12 79 535.2 0 79 13 90 535.2 0 90 14 73 535.2 0 73 15 78 535.2 0 78 16 69 535.2 0 69 17 80 535.2 0 80 18 87 535.2 0 87 19 88 535.2 0 88 20 81 535.2 0 81 21 76 535.2 0 76 22 103 535.2 0 103 23 82 535.2 0 82 24 62 535.2 0 62 25 63 535.2 0 63 26 80 535.2 0 80 27 88 535.2 0 88 28 82 535.2 0 82 29 89 535.2 0 89 30 94 535.2 0 94 31 80 535.2 0 80 32 87 535.2 0 87 33 84 535.2 0 84 34 60 535.2 0 60 35 106 535.2 0 106 36 88 535.2 0 88 37 73 535.2 0 73 38 101 535.2 0 101 39 186 535.2 0 186 40 327 535.2 0 327 41 397 535.2 0 397 42 74 535.2 0 74 43 95 535.2 0 95 44 59 535.2 0 59 45 80 535.2 0 80 46 93 535.2 0 93 47 69 535.2 0 69 48 74 535.2 0 74 49 81 535.2 0 81 50 74 535.2 0 74 51 74 535.2 0 74 52 106 535.2 0 106 53 95 535.2 0 95 54 103 535.2 0 103 55 75 535.2 0 75 56 95 535.2 0 95 57 96 535.2 0 96 58 97 535.2 0 97 59 71 535.2 0 71 60 99 535.2 0 99 61 63 535.2 0 63 62 72 535.2 0 72 63 74 535.2 0 74 64 77 535.2 0 77 65 291 535.2 0 291 66 569 535.2 0 569 67 94 535.2 0 94 68 99 535.2 0 99 69 77 535.2 0 77 70 81 535.2 0 81 71 97 535.2 0 97 72 124 535.2 0 124 73 103 535.2 0 103 74 117 535.2 0 117 75 91 535.2 0 91 76 74 535.2 0 74 77 88 535.2 0 88 78 97 535.2 0 97 79 90 535.2 0 90 80 111 535.2 0 111 81 87 535.2 0 87 82 85 535.2 0 85 83 88 535.2 0 88 84 98 535.2 0 98 85 90 535.2 0 90 86 86 535.2 0 86 87 72 535.2 0 72 88 67 535.2 0 67 89 65 535.2 0 65 90 78 535.2 0 78 91 108 535.2 0 108 92 114 535.2 0 114 93 119 535.2 0 119 94 69 535.2 0 69 95 93 535.2 0 93 96 94 535.2 0 94 97 92 535.2 0 92 98 207 535.2 0 207 99 298 535.2 0 298 100 283 535.2 0 283 101 90 535.2 0 90 102 150 535.2 0 150 103 88 535.2 0 88 104 88 535.2 0 88 105 95 535.2 0 95 106 82 535.2 0 82 107 116 535.2 0 116 108 98 535.2 0 98 109 109 535.2 0 109 110 93 535.2 0 93 111 291 535.2 0 291 112 241 535.2 0 241 113 177 535.2 0 177 114 97 535.2 0 97 115 79 535.2 0 79 116 108 535.2 0 108 117 86 535.2 0 86 118 78 535.2 0 78 119 70 535.2 0 70 120 81 535.2 0 81 121 99 535.2 0 99 122 76 535.2 0 76 123 74 535.2 0 74 124 122 535.2 0 122 125 116 535.2 0 116 126 97 535.2 0 97 127 87 535.2 0 87 128 82 535.2 0 82 129 76 535.2 0 76 130 82 535.2 0 82 131 115 535.2 0 115 132 87 535.2 0 87 133 88 535.2 0 88 134 93 535.2 0 93 135 126 535.2 0 126 136 88 535.2 0 88 137 90 535.2 0 90 138 88 535.2 0 88 139 87 535.2 0 87 140 71 535.2 0 71 141 56 535.2 0 56 142 99 535.2 0 99 143 108 535.2 0 108 144 127 535.2 0 127 145 86 535.2 0 86 146 125 535.2 0 125 147 155 535.2 0 155 148 160 535.2 0 160 149 116 535.2 0 116 150 96 535.2 0 96 151 94 535.2 0 94 152 95 535.2 0 95 153 99 535.2 0 99 154 74 535.2 0 74 155 90 535.2 0 90 156 99 535.2 0 99 157 94 535.2 0 94 158 145 535.2 0 145 159 146 535.2 0 146 160 159 535.2 0 159 161 79 535.2 0 79 162 95 535.2 0 95 163 103 535.2 0 103 164 97 535.2 0 97 165 88 535.2 0 88 166 95 535.2 0 95 167 102 535.2 0 102 168 95 535.2 0 95 169 107 535.2 0 107 170 107 535.2 0 107 171 120 535.2 0 120 172 153 535.2 0 153 173 153 535.2 0 153 174 92 535.2 0 92 175 73 535.2 0 73 176 92 535.2 0 92 177 129 535.2 0 129 178 137 535.2 0 137 179 151 535.2 0 151 180 137 535.2 0 137 181 98 535.2 0 98 182 98 535.2 0 98 183 274 535.2 0 274 184 378 535.2 0 378 185 262 535.2 0 262 186 115 535.2 0 115 187 103 535.2 0 103 188 97 535.2 0 97 189 122 535.2 0 122 190 109 535.2 0 109 191 130 535.2 0 130 192 118 535.2 0 118 193 99 535.2 0 99 194 79 535.2 0 79 195 104 535.2 0 104 196 96 535.2 0 96 197 87 535.2 0 87 198 72 535.2 0 72 199 88 535.2 0 88 200 91 535.2 0 91 201 103 535.2 0 103 202 82 535.2 0 82 203 94 535.2 0 94 204 113 535.2 0 113 205 85 535.2 0 85 206 71 535.2 0 71 207 96 535.2 0 96 208 59 535.2 0 59 209 61 535.2 0 61 210 108 535.2 0 108 211 157 535.2 0 157 212 127 535.2 0 127 213 80 535.2 0 80 214 173 535.2 0 173 215 317 535.2 0 317 216 229 535.2 0 229 217 124 535.2 0 124 218 67 535.2 0 67 219 87 535.2 0 87 220 162 535.2 0 162 221 104 535.2 0 104 222 153 535.2 0 153 223 720 535.2 0 720 224 864 535.2 0 864 225 698 535.2 0 698 226 86 535.2 0 86 227 98 535.2 0 98 228 162 535.2 0 162 229 158 535.2 0 158 230 79 535.2 0 79 231 96 535.2 0 96 232 92 535.2 0 92 233 69 535.2 0 69 234 89 535.2 0 89 235 90 535.2 0 90 236 91 535.2 0 91 237 102 535.2 0 102 238 423 535.2 0 423 239 305 535.2 0 305 240 230 535.2 0 230 241 110 535.2 0 110 242 122 535.2 0 122 243 102 535.2 0 102 244 61 535.2 0 61 245 108 535.2 0 108 246 78 535.2 0 78 247 83 535.2 0 83 248 89 535.2 0 89 249 97 535.2 0 97 250 93 535.2 0 93 251 92 535.2 0 92 252 83 535.2 0 83 253 95 535.2 0 95 254 153 535.2 0 153 255 80 535.2 0 80 256 144 535.2 0 144 257 107 535.2 0 107 258 85 535.2 0 85 259 108 535.2 0 108 260 149 535.2 0 149 261 173 535.2 0 173 262 100 535.2 0 100 263 243 535.2 0 243 264 279 535.2 0 279 265 300 535.2 0 300 266 90 535.2 0 90 267 131 535.2 0 131 268 79 535.2 0 79 269 109 535.2 0 109 270 108 535.2 0 108 271 218 535.2 0 218 272 1619 535.2 0 1619 273 932 535.2 0 932 274 1312 535.2 0 1312 275 128 535.2 0 128 276 101 535.2 0 101 277 181 535.2 0 181 278 310 535.2 0 310 279 148 535.2 0 148 280 165 535.2 0 165 281 85 535.2 0 85 282 99 535.2 0 99 283 152 535.2 0 152 284 242 535.2 0 242 285 333 535.2 0 333 286 206 535.2 0 206 287 82 535.2 0 82 288 117 535.2 0 117 289 103 535.2 0 103 290 88 535.2 0 88 291 60 535.2 0 60 292 88 535.2 0 88 293 68 535.2 0 68 294 88 535.2 0 88 295 102 535.2 0 102 296 65 535.2 0 65 297 135 535.2 0 135 298 151 535.2 0 151 299 291 535.2 0 291 300 190 535.2 0 190 301 111 535.2 0 111 Finished in 5.92 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 117,052 Reads with adapters: 53,338 (45.6%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 53,321 (45.6%) Total basepairs processed: 32,222,570 bp Total written (filtered): 14,541,309 bp (45.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53338 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 21 0.0 3 0 0 20 1 19 510 0.0 3 0 503 6 1 20 52423 0.0 4 51627 770 25 1 21 158 0.0 4 31 123 4 22 10 0.0 4 1 0 9 23 36 0.0 4 0 0 0 34 2 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 1 27 1 0.0 4 1 29 1 0.0 4 1 30 2 0.0 4 1 0 1 36 8 0.0 4 0 0 0 0 8 72 1 0.0 4 1 82 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 145 0.0 4 0 0 0 0 145 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 213 1 0.0 4 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.86 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 53,321 Reads with adapters: 4,570 (8.6%) Reads written (passing filters): 53,321 (100.0%) Total basepairs processed: 14,541,309 bp Total written (filtered): 14,486,332 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4570 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 4.3% G: 92.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 833.1 0 34 4 8 208.3 0 8 7 1 3.3 0 1 9 41 0.2 0 2 39 10 653 0.1 1 26 627 11 3791 0.0 1 25 3766 12 5 0.0 1 0 5 13 1 0.0 1 0 1 38 1 0.0 1 1 148 2 0.0 1 2 151 18 0.0 1 2 16 152 1 0.0 1 0 1 153 1 0.0 1 0 1 162 1 0.0 1 1 209 3 0.0 1 2 1 224 8 0.0 1 8 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.