This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.97 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 242,246 (11.0%) Reads that were too short: 22,391 (1.0%) Reads written (passing filters): 219,855 (10.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 41,932,583 bp (6.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 242246 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 535.2 0 652 7 8931 535.2 0 8931 8 486 535.2 0 486 9 10059 535.2 0 10059 10 163 535.2 0 163 11 193 535.2 0 193 12 153 535.2 0 153 13 134 535.2 0 134 14 136 535.2 0 136 15 140 535.2 0 140 16 148 535.2 0 148 17 184 535.2 0 184 18 172 535.2 0 172 19 154 535.2 0 154 20 183 535.2 0 183 21 431 535.2 0 431 22 556 535.2 0 556 23 490 535.2 0 490 24 1238 535.2 0 1238 25 2250 535.2 0 2250 26 2596 535.2 0 2596 27 150 535.2 0 150 28 166 535.2 0 166 29 137 535.2 0 137 30 124 535.2 0 124 31 144 535.2 0 144 32 136 535.2 0 136 33 133 535.2 0 133 34 130 535.2 0 130 35 103 535.2 0 103 36 141 535.2 0 141 37 107 535.2 0 107 38 118 535.2 0 118 39 127 535.2 0 127 40 131 535.2 0 131 41 146 535.2 0 146 42 115 535.2 0 115 43 126 535.2 0 126 44 140 535.2 0 140 45 131 535.2 0 131 46 142 535.2 0 142 47 126 535.2 0 126 48 144 535.2 0 144 49 127 535.2 0 127 50 135 535.2 0 135 51 166 535.2 0 166 52 121 535.2 0 121 53 143 535.2 0 143 54 198 535.2 0 198 55 212 535.2 0 212 56 157 535.2 0 157 57 164 535.2 0 164 58 181 535.2 0 181 59 154 535.2 0 154 60 143 535.2 0 143 61 533 535.2 0 533 62 1095 535.2 0 1095 63 700 535.2 0 700 64 136 535.2 0 136 65 118 535.2 0 118 66 157 535.2 0 157 67 162 535.2 0 162 68 152 535.2 0 152 69 283 535.2 0 283 70 415 535.2 0 415 71 371 535.2 0 371 72 227 535.2 0 227 73 190 535.2 0 190 74 239 535.2 0 239 75 175 535.2 0 175 76 163 535.2 0 163 77 185 535.2 0 185 78 231 535.2 0 231 79 241 535.2 0 241 80 279 535.2 0 279 81 259 535.2 0 259 82 418 535.2 0 418 83 1936 535.2 0 1936 84 3582 535.2 0 3582 85 2605 535.2 0 2605 86 230 535.2 0 230 87 185 535.2 0 185 88 309 535.2 0 309 89 159 535.2 0 159 90 186 535.2 0 186 91 133 535.2 0 133 92 154 535.2 0 154 93 148 535.2 0 148 94 193 535.2 0 193 95 193 535.2 0 193 96 175 535.2 0 175 97 204 535.2 0 204 98 319 535.2 0 319 99 623 535.2 0 623 100 239 535.2 0 239 101 270 535.2 0 270 102 377 535.2 0 377 103 271 535.2 0 271 104 246 535.2 0 246 105 440 535.2 0 440 106 449 535.2 0 449 107 595 535.2 0 595 108 675 535.2 0 675 109 887 535.2 0 887 110 1011 535.2 0 1011 111 1312 535.2 0 1312 112 19222 535.2 0 19222 113 41113 535.2 0 41113 114 32051 535.2 0 32051 115 293 535.2 0 293 116 145 535.2 0 145 117 201 535.2 0 201 118 286 535.2 0 286 119 346 535.2 0 346 120 2094 535.2 0 2094 121 2200 535.2 0 2200 122 2477 535.2 0 2477 123 274 535.2 0 274 124 298 535.2 0 298 125 951 535.2 0 951 126 1067 535.2 0 1067 127 1109 535.2 0 1109 128 238 535.2 0 238 129 421 535.2 0 421 130 303 535.2 0 303 131 317 535.2 0 317 132 143 535.2 0 143 133 173 535.2 0 173 134 195 535.2 0 195 135 176 535.2 0 176 136 234 535.2 0 234 137 357 535.2 0 357 138 374 535.2 0 374 139 351 535.2 0 351 140 291 535.2 0 291 141 262 535.2 0 262 142 693 535.2 0 693 143 1164 535.2 0 1164 144 925 535.2 0 925 145 404 535.2 0 404 146 434 535.2 0 434 147 1627 535.2 0 1627 148 2916 535.2 0 2916 149 3005 535.2 0 3005 150 904 535.2 0 904 151 335 535.2 0 335 152 388 535.2 0 388 153 217 535.2 0 217 154 159 535.2 0 159 155 179 535.2 0 179 156 186 535.2 0 186 157 189 535.2 0 189 158 194 535.2 0 194 159 192 535.2 0 192 160 237 535.2 0 237 161 272 535.2 0 272 162 342 535.2 0 342 163 324 535.2 0 324 164 449 535.2 0 449 165 579 535.2 0 579 166 1042 535.2 0 1042 167 2023 535.2 0 2023 168 2318 535.2 0 2318 169 957 535.2 0 957 170 884 535.2 0 884 171 1999 535.2 0 1999 172 2053 535.2 0 2053 173 2192 535.2 0 2192 174 476 535.2 0 476 175 321 535.2 0 321 176 277 535.2 0 277 177 223 535.2 0 223 178 237 535.2 0 237 179 198 535.2 0 198 180 229 535.2 0 229 181 273 535.2 0 273 182 404 535.2 0 404 183 2162 535.2 0 2162 184 2371 535.2 0 2371 185 2256 535.2 0 2256 186 635 535.2 0 635 187 479 535.2 0 479 188 539 535.2 0 539 189 454 535.2 0 454 190 291 535.2 0 291 191 1341 535.2 0 1341 192 2620 535.2 0 2620 193 2766 535.2 0 2766 194 953 535.2 0 953 195 381 535.2 0 381 196 428 535.2 0 428 197 231 535.2 0 231 198 242 535.2 0 242 199 1467 535.2 0 1467 200 397 535.2 0 397 201 358 535.2 0 358 202 244 535.2 0 244 203 223 535.2 0 223 204 147 535.2 0 147 205 179 535.2 0 179 206 209 535.2 0 209 207 231 535.2 0 231 208 286 535.2 0 286 209 205 535.2 0 205 210 189 535.2 0 189 211 150 535.2 0 150 212 175 535.2 0 175 213 162 535.2 0 162 214 140 535.2 0 140 215 188 535.2 0 188 216 242 535.2 0 242 217 196 535.2 0 196 218 267 535.2 0 267 219 310 535.2 0 310 220 240 535.2 0 240 221 156 535.2 0 156 222 178 535.2 0 178 223 156 535.2 0 156 224 185 535.2 0 185 225 269 535.2 0 269 226 416 535.2 0 416 227 363 535.2 0 363 228 572 535.2 0 572 229 390 535.2 0 390 230 314 535.2 0 314 231 164 535.2 0 164 232 167 535.2 0 167 233 154 535.2 0 154 234 159 535.2 0 159 235 175 535.2 0 175 236 132 535.2 0 132 237 178 535.2 0 178 238 196 535.2 0 196 239 211 535.2 0 211 240 161 535.2 0 161 241 141 535.2 0 141 242 161 535.2 0 161 243 182 535.2 0 182 244 231 535.2 0 231 245 261 535.2 0 261 246 461 535.2 0 461 247 356 535.2 0 356 248 192 535.2 0 192 249 134 535.2 0 134 250 133 535.2 0 133 251 240 535.2 0 240 252 212 535.2 0 212 253 230 535.2 0 230 254 179 535.2 0 179 255 212 535.2 0 212 256 258 535.2 0 258 257 204 535.2 0 204 258 208 535.2 0 208 259 162 535.2 0 162 260 167 535.2 0 167 261 172 535.2 0 172 262 170 535.2 0 170 263 179 535.2 0 179 264 168 535.2 0 168 265 191 535.2 0 191 266 198 535.2 0 198 267 711 535.2 0 711 268 907 535.2 0 907 269 591 535.2 0 591 270 209 535.2 0 209 271 153 535.2 0 153 272 143 535.2 0 143 273 145 535.2 0 145 274 139 535.2 0 139 275 176 535.2 0 176 276 205 535.2 0 205 277 295 535.2 0 295 278 248 535.2 0 248 279 278 535.2 0 278 280 191 535.2 0 191 281 194 535.2 0 194 282 230 535.2 0 230 283 160 535.2 0 160 284 107 535.2 0 107 285 116 535.2 0 116 286 133 535.2 0 133 287 147 535.2 0 147 288 138 535.2 0 138 289 181 535.2 0 181 290 146 535.2 0 146 291 143 535.2 0 143 292 139 535.2 0 139 293 183 535.2 0 183 294 212 535.2 0 212 295 206 535.2 0 206 296 162 535.2 0 162 297 312 535.2 0 312 298 279 535.2 0 279 299 427 535.2 0 427 300 134 535.2 0 134 301 170 535.2 0 170 Finished in 7.91 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 219,855 Reads with adapters: 13,186 (6.0%) Reads that were too short: 941 (0.4%) Reads written (passing filters): 12,245 (5.6%) Total basepairs processed: 41,932,583 bp Total written (filtered): 3,100,948 bp (7.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13186 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 8 0.0 3 0 0 5 3 16 105 0.0 3 0 79 14 12 17 10182 0.0 3 9265 733 144 40 18 144 0.0 3 63 43 16 22 19 37 0.0 3 0 0 17 20 20 22 0.0 3 5 1 2 14 21 19 0.0 3 0 0 1 18 22 1 0.0 3 0 0 0 1 23 4 0.0 3 0 0 0 4 24 391 0.0 3 0 0 3 388 25 1 0.0 3 0 0 0 1 26 52 0.0 3 0 0 0 52 27 84 0.0 3 0 0 3 81 28 26 0.0 3 1 0 1 24 29 2 0.0 3 0 0 0 2 30 6 0.0 3 0 0 0 6 31 12 0.0 3 0 0 2 10 33 2 0.0 3 0 0 0 2 35 3 0.0 3 0 1 0 2 36 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 4 0.0 3 0 0 1 3 40 3 0.0 3 0 0 0 3 42 4 0.0 3 0 0 0 4 45 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 47 7 0.0 3 0 0 0 7 48 11 0.0 3 0 0 2 9 49 2 0.0 3 0 0 0 2 50 19 0.0 3 0 0 0 19 51 134 0.0 3 0 0 3 131 52 2 0.0 3 0 0 0 2 53 7 0.0 3 0 0 1 6 54 40 0.0 3 0 0 1 39 55 3 0.0 3 0 0 0 3 56 2 0.0 3 0 1 0 1 57 4 0.0 3 0 0 0 4 58 4 0.0 3 0 0 1 3 59 17 0.0 3 0 0 1 16 60 17 0.0 3 0 0 1 16 61 10 0.0 3 0 0 1 9 62 8 0.0 3 0 0 1 7 63 19 0.0 3 0 0 1 18 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 45 0.0 3 0 0 0 45 67 7 0.0 3 0 0 0 7 68 18 0.0 3 0 0 2 16 69 16 0.0 3 0 0 1 15 70 3 0.0 3 0 0 0 3 71 7 0.0 3 0 0 0 7 72 4 0.0 3 0 0 0 4 74 5 0.0 3 0 0 1 4 75 18 0.0 3 0 0 1 17 76 17 0.0 3 0 0 0 17 77 9 0.0 3 0 0 0 9 78 8 0.0 3 0 0 0 8 79 226 0.0 3 0 0 3 223 80 5 0.0 3 0 0 0 5 81 6 0.0 3 0 0 0 6 82 51 0.0 3 0 0 1 50 83 9 0.0 3 0 0 0 9 84 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 88 3 0.0 3 0 0 0 3 89 4 0.0 3 0 0 0 4 90 2 0.0 3 0 0 1 1 92 4 0.0 3 0 0 0 4 93 6 0.0 3 0 0 0 6 95 5 0.0 3 0 0 0 5 96 6 0.0 3 0 0 1 5 97 5 0.0 3 0 0 1 4 98 16 0.0 3 0 0 0 16 99 5 0.0 3 0 0 1 4 100 4 0.0 3 0 0 1 3 101 21 0.0 3 0 0 0 21 102 27 0.0 3 0 0 1 26 103 5 0.0 3 0 0 1 4 104 6 0.0 3 0 0 0 6 105 25 0.0 3 0 0 2 23 106 3 0.0 3 0 0 0 3 108 81 0.0 3 0 0 0 81 109 43 0.0 3 0 0 0 43 110 25 0.0 3 0 0 2 23 111 18 0.0 3 0 0 1 17 112 25 0.0 3 0 0 1 24 113 8 0.0 3 0 0 0 8 114 14 0.0 3 0 0 2 12 115 10 0.0 3 0 0 1 9 116 3 0.0 3 0 0 0 3 117 11 0.0 3 0 0 0 11 119 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 7 0.0 3 0 0 1 6 123 6 0.0 3 0 0 1 5 124 6 0.0 3 0 0 0 6 125 9 0.0 3 0 0 0 9 126 3 0.0 3 0 0 0 3 127 7 0.0 3 1 0 1 5 128 3 0.0 3 0 0 0 3 129 8 0.0 3 0 0 1 7 130 69 0.0 3 0 0 4 65 131 7 0.0 3 0 0 0 7 132 12 0.0 3 0 0 1 11 133 3 0.0 3 0 0 0 3 134 9 0.0 3 0 0 1 8 135 3 0.0 3 0 0 0 3 136 15 0.0 3 0 0 1 14 137 3 0.0 3 0 0 0 3 138 3 0.0 3 0 0 0 3 139 19 0.0 3 0 0 0 19 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 10 0.0 3 0 0 0 10 143 1 0.0 3 0 0 0 1 144 8 0.0 3 0 0 0 8 145 10 0.0 3 0 0 2 8 146 1 0.0 3 0 0 0 1 147 40 0.0 3 0 0 2 38 148 6 0.0 3 0 0 0 6 149 2 0.0 3 0 0 0 2 150 17 0.0 3 0 0 0 17 151 3 0.0 3 0 0 1 2 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 7 0.0 3 0 0 1 6 155 2 0.0 3 0 0 0 2 156 4 0.0 3 0 0 0 4 158 2 0.0 3 0 0 0 2 159 9 0.0 3 0 0 0 9 160 5 0.0 3 0 0 1 4 161 3 0.0 3 0 0 0 3 162 42 0.0 3 0 0 0 42 163 4 0.0 3 0 0 0 4 164 5 0.0 3 0 0 0 5 165 6 0.0 3 0 0 0 6 166 3 0.0 3 0 0 0 3 167 43 0.0 3 0 0 0 43 168 34 0.0 3 0 0 1 33 169 5 0.0 3 0 0 0 5 170 40 0.0 3 0 0 2 38 171 20 0.0 3 0 0 0 20 172 4 0.0 3 0 0 1 3 173 8 0.0 3 0 0 0 8 174 5 0.0 3 0 0 0 5 176 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 5 0.0 3 0 0 0 5 180 43 0.0 3 0 0 2 41 181 133 0.0 3 0 0 7 126 182 6 0.0 3 0 0 0 6 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 190 3 0.0 3 0 0 0 3 191 6 0.0 3 0 0 0 6 192 1 0.0 3 0 0 0 1 193 5 0.0 3 0 0 0 5 194 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 1 1 199 3 0.0 3 0 0 0 3 200 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 202 3 0.0 3 0 0 0 3 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 209 5 0.0 3 0 0 0 5 210 13 0.0 3 0 0 2 11 211 4 0.0 3 0 0 0 4 213 3 0.0 3 0 0 0 3 215 3 0.0 3 0 0 0 3 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 1 1 218 6 0.0 3 0 0 0 6 219 2 0.0 3 0 0 0 2 220 5 0.0 3 0 0 0 5 221 4 0.0 3 0 0 0 4 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 231 4 0.0 3 0 0 0 4 232 3 0.0 3 0 0 0 3 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 257 2 0.0 3 0 0 0 2 260 2 0.0 3 0 0 0 2 261 1 0.0 3 0 0 0 1 262 4 0.0 3 0 0 0 4 263 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 265 2 0.0 3 0 0 0 2 267 4 0.0 3 0 0 0 4 268 4 0.0 3 0 0 2 2 269 9 0.0 3 0 0 2 7 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 12,245 Reads with adapters: 215 (1.8%) Reads written (passing filters): 12,245 (100.0%) Total basepairs processed: 3,100,948 bp Total written (filtered): 3,100,295 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 215 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.1% C: 14.4% G: 18.1% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 207 191.3 0 207 4 8 47.8 0 8