This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.59 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 216,753 (9.9%) Reads that were too short: 19,968 (0.9%) Reads written (passing filters): 196,785 (9.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 38,148,440 bp (5.8%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 216753 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 419 535.2 0 419 7 11267 535.2 0 11267 8 343 535.2 0 343 9 9031 535.2 0 9031 10 174 535.2 0 174 11 216 535.2 0 216 12 175 535.2 0 175 13 153 535.2 0 153 14 186 535.2 0 186 15 199 535.2 0 199 16 176 535.2 0 176 17 215 535.2 0 215 18 172 535.2 0 172 19 160 535.2 0 160 20 154 535.2 0 154 21 209 535.2 0 209 22 229 535.2 0 229 23 295 535.2 0 295 24 738 535.2 0 738 25 459 535.2 0 459 26 547 535.2 0 547 27 167 535.2 0 167 28 177 535.2 0 177 29 141 535.2 0 141 30 174 535.2 0 174 31 144 535.2 0 144 32 172 535.2 0 172 33 209 535.2 0 209 34 168 535.2 0 168 35 140 535.2 0 140 36 166 535.2 0 166 37 163 535.2 0 163 38 173 535.2 0 173 39 155 535.2 0 155 40 148 535.2 0 148 41 190 535.2 0 190 42 165 535.2 0 165 43 157 535.2 0 157 44 191 535.2 0 191 45 165 535.2 0 165 46 178 535.2 0 178 47 202 535.2 0 202 48 148 535.2 0 148 49 183 535.2 0 183 50 182 535.2 0 182 51 181 535.2 0 181 52 139 535.2 0 139 53 171 535.2 0 171 54 174 535.2 0 174 55 170 535.2 0 170 56 145 535.2 0 145 57 188 535.2 0 188 58 198 535.2 0 198 59 198 535.2 0 198 60 165 535.2 0 165 61 341 535.2 0 341 62 560 535.2 0 560 63 358 535.2 0 358 64 144 535.2 0 144 65 143 535.2 0 143 66 163 535.2 0 163 67 158 535.2 0 158 68 159 535.2 0 159 69 190 535.2 0 190 70 224 535.2 0 224 71 203 535.2 0 203 72 186 535.2 0 186 73 194 535.2 0 194 74 175 535.2 0 175 75 166 535.2 0 166 76 195 535.2 0 195 77 197 535.2 0 197 78 236 535.2 0 236 79 214 535.2 0 214 80 218 535.2 0 218 81 204 535.2 0 204 82 268 535.2 0 268 83 687 535.2 0 687 84 1228 535.2 0 1228 85 773 535.2 0 773 86 232 535.2 0 232 87 239 535.2 0 239 88 382 535.2 0 382 89 224 535.2 0 224 90 190 535.2 0 190 91 152 535.2 0 152 92 216 535.2 0 216 93 193 535.2 0 193 94 183 535.2 0 183 95 234 535.2 0 234 96 188 535.2 0 188 97 242 535.2 0 242 98 386 535.2 0 386 99 701 535.2 0 701 100 253 535.2 0 253 101 332 535.2 0 332 102 434 535.2 0 434 103 264 535.2 0 264 104 250 535.2 0 250 105 509 535.2 0 509 106 504 535.2 0 504 107 571 535.2 0 571 108 653 535.2 0 653 109 847 535.2 0 847 110 974 535.2 0 974 111 1258 535.2 0 1258 112 20078 535.2 0 20078 113 46838 535.2 0 46838 114 34788 535.2 0 34788 115 295 535.2 0 295 116 154 535.2 0 154 117 165 535.2 0 165 118 225 535.2 0 225 119 274 535.2 0 274 120 1118 535.2 0 1118 121 1521 535.2 0 1521 122 1295 535.2 0 1295 123 199 535.2 0 199 124 243 535.2 0 243 125 894 535.2 0 894 126 1055 535.2 0 1055 127 1159 535.2 0 1159 128 284 535.2 0 284 129 479 535.2 0 479 130 309 535.2 0 309 131 364 535.2 0 364 132 176 535.2 0 176 133 171 535.2 0 171 134 193 535.2 0 193 135 194 535.2 0 194 136 199 535.2 0 199 137 217 535.2 0 217 138 287 535.2 0 287 139 311 535.2 0 311 140 243 535.2 0 243 141 225 535.2 0 225 142 462 535.2 0 462 143 509 535.2 0 509 144 410 535.2 0 410 145 203 535.2 0 203 146 233 535.2 0 233 147 493 535.2 0 493 148 947 535.2 0 947 149 1005 535.2 0 1005 150 453 535.2 0 453 151 186 535.2 0 186 152 257 535.2 0 257 153 198 535.2 0 198 154 187 535.2 0 187 155 171 535.2 0 171 156 153 535.2 0 153 157 193 535.2 0 193 158 174 535.2 0 174 159 170 535.2 0 170 160 150 535.2 0 150 161 177 535.2 0 177 162 223 535.2 0 223 163 209 535.2 0 209 164 230 535.2 0 230 165 241 535.2 0 241 166 541 535.2 0 541 167 802 535.2 0 802 168 998 535.2 0 998 169 484 535.2 0 484 170 445 535.2 0 445 171 1123 535.2 0 1123 172 971 535.2 0 971 173 1265 535.2 0 1265 174 314 535.2 0 314 175 214 535.2 0 214 176 194 535.2 0 194 177 189 535.2 0 189 178 224 535.2 0 224 179 184 535.2 0 184 180 260 535.2 0 260 181 263 535.2 0 263 182 379 535.2 0 379 183 2501 535.2 0 2501 184 2800 535.2 0 2800 185 2774 535.2 0 2774 186 353 535.2 0 353 187 268 535.2 0 268 188 303 535.2 0 303 189 204 535.2 0 204 190 196 535.2 0 196 191 612 535.2 0 612 192 1182 535.2 0 1182 193 1082 535.2 0 1082 194 487 535.2 0 487 195 222 535.2 0 222 196 244 535.2 0 244 197 218 535.2 0 218 198 259 535.2 0 259 199 2258 535.2 0 2258 200 447 535.2 0 447 201 439 535.2 0 439 202 250 535.2 0 250 203 298 535.2 0 298 204 142 535.2 0 142 205 167 535.2 0 167 206 176 535.2 0 176 207 195 535.2 0 195 208 187 535.2 0 187 209 178 535.2 0 178 210 173 535.2 0 173 211 167 535.2 0 167 212 137 535.2 0 137 213 191 535.2 0 191 214 161 535.2 0 161 215 239 535.2 0 239 216 228 535.2 0 228 217 237 535.2 0 237 218 198 535.2 0 198 219 196 535.2 0 196 220 167 535.2 0 167 221 136 535.2 0 136 222 195 535.2 0 195 223 144 535.2 0 144 224 158 535.2 0 158 225 213 535.2 0 213 226 274 535.2 0 274 227 271 535.2 0 271 228 415 535.2 0 415 229 327 535.2 0 327 230 280 535.2 0 280 231 156 535.2 0 156 232 193 535.2 0 193 233 169 535.2 0 169 234 149 535.2 0 149 235 179 535.2 0 179 236 154 535.2 0 154 237 185 535.2 0 185 238 184 535.2 0 184 239 192 535.2 0 192 240 141 535.2 0 141 241 169 535.2 0 169 242 161 535.2 0 161 243 168 535.2 0 168 244 233 535.2 0 233 245 197 535.2 0 197 246 281 535.2 0 281 247 187 535.2 0 187 248 159 535.2 0 159 249 167 535.2 0 167 250 136 535.2 0 136 251 176 535.2 0 176 252 164 535.2 0 164 253 173 535.2 0 173 254 154 535.2 0 154 255 164 535.2 0 164 256 166 535.2 0 166 257 177 535.2 0 177 258 213 535.2 0 213 259 178 535.2 0 178 260 142 535.2 0 142 261 159 535.2 0 159 262 153 535.2 0 153 263 178 535.2 0 178 264 174 535.2 0 174 265 187 535.2 0 187 266 196 535.2 0 196 267 793 535.2 0 793 268 973 535.2 0 973 269 604 535.2 0 604 270 153 535.2 0 153 271 164 535.2 0 164 272 123 535.2 0 123 273 122 535.2 0 122 274 170 535.2 0 170 275 169 535.2 0 169 276 210 535.2 0 210 277 268 535.2 0 268 278 206 535.2 0 206 279 184 535.2 0 184 280 143 535.2 0 143 281 172 535.2 0 172 282 178 535.2 0 178 283 167 535.2 0 167 284 138 535.2 0 138 285 124 535.2 0 124 286 138 535.2 0 138 287 144 535.2 0 144 288 148 535.2 0 148 289 145 535.2 0 145 290 162 535.2 0 162 291 145 535.2 0 145 292 156 535.2 0 156 293 148 535.2 0 148 294 166 535.2 0 166 295 155 535.2 0 155 296 139 535.2 0 139 297 167 535.2 0 167 298 157 535.2 0 157 299 256 535.2 0 256 300 134 535.2 0 134 301 133 535.2 0 133 Finished in 8.04 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 196,785 Reads with adapters: 11,388 (5.8%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 11,356 (5.8%) Total basepairs processed: 38,148,440 bp Total written (filtered): 3,094,617 bp (8.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11388 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 2 0 1 19 109 0.0 3 0 107 2 20 11035 0.0 4 10856 170 7 1 1 21 42 0.0 4 22 20 22 3 0.0 4 0 0 1 0 2 23 8 0.0 4 4 0 0 3 1 24 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 49 3 0.0 4 0 0 0 0 3 50 1 0.0 4 0 0 0 0 1 51 40 0.0 4 0 0 0 0 40 54 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 9 0.0 4 0 0 0 0 9 113 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 92 0.0 4 0 0 0 0 92 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 1 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 177 5 0.0 4 0 0 0 0 5 178 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 1 220 1 0.0 4 0 0 1 221 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,356 Reads with adapters: 595 (5.2%) Reads written (passing filters): 11,356 (100.0%) Total basepairs processed: 3,094,617 bp Total written (filtered): 3,044,529 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 595 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 3.2% G: 32.4% T: 62.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 177.4 0 17 4 3 44.4 0 3 9 1 0.0 0 0 1 10 6 0.0 1 0 6 11 176 0.0 1 3 173 12 18 0.0 1 0 18 126 1 0.0 1 1 127 356 0.0 1 323 33 128 14 0.0 1 11 3 140 1 0.0 1 1 267 2 0.0 1 1 1