This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.18 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 173,554 (7.9%) Reads that were too short: 42,480 (1.9%) Reads written (passing filters): 131,074 (6.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,522,091 bp (5.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 173554 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 573 535.2 0 573 7 1418 535.2 0 1418 8 59739 535.2 0 59739 9 42296 535.2 0 42296 10 173 535.2 0 173 11 101 535.2 0 101 12 111 535.2 0 111 13 97 535.2 0 97 14 133 535.2 0 133 15 128 535.2 0 128 16 123 535.2 0 123 17 142 535.2 0 142 18 121 535.2 0 121 19 108 535.2 0 108 20 121 535.2 0 121 21 108 535.2 0 108 22 128 535.2 0 128 23 109 535.2 0 109 24 103 535.2 0 103 25 95 535.2 0 95 26 137 535.2 0 137 27 126 535.2 0 126 28 114 535.2 0 114 29 128 535.2 0 128 30 118 535.2 0 118 31 101 535.2 0 101 32 105 535.2 0 105 33 122 535.2 0 122 34 120 535.2 0 120 35 108 535.2 0 108 36 106 535.2 0 106 37 123 535.2 0 123 38 100 535.2 0 100 39 95 535.2 0 95 40 88 535.2 0 88 41 98 535.2 0 98 42 103 535.2 0 103 43 112 535.2 0 112 44 159 535.2 0 159 45 111 535.2 0 111 46 116 535.2 0 116 47 107 535.2 0 107 48 116 535.2 0 116 49 107 535.2 0 107 50 111 535.2 0 111 51 109 535.2 0 109 52 129 535.2 0 129 53 136 535.2 0 136 54 129 535.2 0 129 55 101 535.2 0 101 56 107 535.2 0 107 57 101 535.2 0 101 58 108 535.2 0 108 59 139 535.2 0 139 60 188 535.2 0 188 61 171 535.2 0 171 62 108 535.2 0 108 63 105 535.2 0 105 64 97 535.2 0 97 65 92 535.2 0 92 66 112 535.2 0 112 67 125 535.2 0 125 68 86 535.2 0 86 69 102 535.2 0 102 70 97 535.2 0 97 71 134 535.2 0 134 72 170 535.2 0 170 73 182 535.2 0 182 74 108 535.2 0 108 75 119 535.2 0 119 76 186 535.2 0 186 77 258 535.2 0 258 78 308 535.2 0 308 79 105 535.2 0 105 80 143 535.2 0 143 81 138 535.2 0 138 82 131 535.2 0 131 83 128 535.2 0 128 84 132 535.2 0 132 85 162 535.2 0 162 86 95 535.2 0 95 87 133 535.2 0 133 88 115 535.2 0 115 89 119 535.2 0 119 90 123 535.2 0 123 91 107 535.2 0 107 92 149 535.2 0 149 93 163 535.2 0 163 94 125 535.2 0 125 95 164 535.2 0 164 96 137 535.2 0 137 97 92 535.2 0 92 98 128 535.2 0 128 99 91 535.2 0 91 100 131 535.2 0 131 101 114 535.2 0 114 102 106 535.2 0 106 103 103 535.2 0 103 104 113 535.2 0 113 105 131 535.2 0 131 106 112 535.2 0 112 107 118 535.2 0 118 108 112 535.2 0 112 109 134 535.2 0 134 110 175 535.2 0 175 111 204 535.2 0 204 112 269 535.2 0 269 113 159 535.2 0 159 114 125 535.2 0 125 115 119 535.2 0 119 116 108 535.2 0 108 117 141 535.2 0 141 118 150 535.2 0 150 119 135 535.2 0 135 120 127 535.2 0 127 121 122 535.2 0 122 122 124 535.2 0 124 123 101 535.2 0 101 124 128 535.2 0 128 125 136 535.2 0 136 126 130 535.2 0 130 127 137 535.2 0 137 128 144 535.2 0 144 129 133 535.2 0 133 130 202 535.2 0 202 131 179 535.2 0 179 132 210 535.2 0 210 133 152 535.2 0 152 134 158 535.2 0 158 135 133 535.2 0 133 136 165 535.2 0 165 137 127 535.2 0 127 138 248 535.2 0 248 139 238 535.2 0 238 140 176 535.2 0 176 141 140 535.2 0 140 142 292 535.2 0 292 143 188 535.2 0 188 144 215 535.2 0 215 145 183 535.2 0 183 146 229 535.2 0 229 147 153 535.2 0 153 148 132 535.2 0 132 149 155 535.2 0 155 150 119 535.2 0 119 151 127 535.2 0 127 152 140 535.2 0 140 153 144 535.2 0 144 154 137 535.2 0 137 155 152 535.2 0 152 156 136 535.2 0 136 157 120 535.2 0 120 158 145 535.2 0 145 159 142 535.2 0 142 160 116 535.2 0 116 161 124 535.2 0 124 162 134 535.2 0 134 163 132 535.2 0 132 164 129 535.2 0 129 165 122 535.2 0 122 166 111 535.2 0 111 167 117 535.2 0 117 168 142 535.2 0 142 169 109 535.2 0 109 170 112 535.2 0 112 171 130 535.2 0 130 172 106 535.2 0 106 173 124 535.2 0 124 174 123 535.2 0 123 175 121 535.2 0 121 176 128 535.2 0 128 177 115 535.2 0 115 178 118 535.2 0 118 179 124 535.2 0 124 180 196 535.2 0 196 181 126 535.2 0 126 182 114 535.2 0 114 183 120 535.2 0 120 184 126 535.2 0 126 185 116 535.2 0 116 186 120 535.2 0 120 187 118 535.2 0 118 188 116 535.2 0 116 189 113 535.2 0 113 190 144 535.2 0 144 191 163 535.2 0 163 192 264 535.2 0 264 193 217 535.2 0 217 194 194 535.2 0 194 195 122 535.2 0 122 196 147 535.2 0 147 197 474 535.2 0 474 198 438 535.2 0 438 199 642 535.2 0 642 200 134 535.2 0 134 201 110 535.2 0 110 202 84 535.2 0 84 203 92 535.2 0 92 204 100 535.2 0 100 205 136 535.2 0 136 206 130 535.2 0 130 207 105 535.2 0 105 208 79 535.2 0 79 209 114 535.2 0 114 210 119 535.2 0 119 211 110 535.2 0 110 212 110 535.2 0 110 213 110 535.2 0 110 214 111 535.2 0 111 215 101 535.2 0 101 216 110 535.2 0 110 217 126 535.2 0 126 218 130 535.2 0 130 219 94 535.2 0 94 220 96 535.2 0 96 221 101 535.2 0 101 222 143 535.2 0 143 223 174 535.2 0 174 224 255 535.2 0 255 225 435 535.2 0 435 226 175 535.2 0 175 227 147 535.2 0 147 228 106 535.2 0 106 229 105 535.2 0 105 230 115 535.2 0 115 231 133 535.2 0 133 232 116 535.2 0 116 233 161 535.2 0 161 234 157 535.2 0 157 235 156 535.2 0 156 236 157 535.2 0 157 237 126 535.2 0 126 238 234 535.2 0 234 239 149 535.2 0 149 240 225 535.2 0 225 241 331 535.2 0 331 242 263 535.2 0 263 243 157 535.2 0 157 244 138 535.2 0 138 245 170 535.2 0 170 246 200 535.2 0 200 247 202 535.2 0 202 248 131 535.2 0 131 249 125 535.2 0 125 250 100 535.2 0 100 251 121 535.2 0 121 252 128 535.2 0 128 253 107 535.2 0 107 254 159 535.2 0 159 255 190 535.2 0 190 256 226 535.2 0 226 257 176 535.2 0 176 258 173 535.2 0 173 259 133 535.2 0 133 260 198 535.2 0 198 261 193 535.2 0 193 262 241 535.2 0 241 263 195 535.2 0 195 264 177 535.2 0 177 265 569 535.2 0 569 266 897 535.2 0 897 267 1044 535.2 0 1044 268 287 535.2 0 287 269 93 535.2 0 93 270 119 535.2 0 119 271 130 535.2 0 130 272 192 535.2 0 192 273 173 535.2 0 173 274 217 535.2 0 217 275 182 535.2 0 182 276 132 535.2 0 132 277 143 535.2 0 143 278 125 535.2 0 125 279 163 535.2 0 163 280 133 535.2 0 133 281 165 535.2 0 165 282 138 535.2 0 138 283 172 535.2 0 172 284 173 535.2 0 173 285 254 535.2 0 254 286 231 535.2 0 231 287 180 535.2 0 180 288 279 535.2 0 279 289 189 535.2 0 189 290 228 535.2 0 228 291 274 535.2 0 274 292 377 535.2 0 377 293 334 535.2 0 334 294 361 535.2 0 361 295 390 535.2 0 390 296 448 535.2 0 448 297 537 535.2 0 537 298 2291 535.2 0 2291 299 4986 535.2 0 4986 300 11132 535.2 0 11132 301 5081 535.2 0 5081 Finished in 6.07 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 131,074 Reads with adapters: 58,192 (44.4%) Reads that were too short: 131 (0.1%) Reads written (passing filters): 58,061 (44.3%) Total basepairs processed: 35,522,091 bp Total written (filtered): 16,013,875 bp (45.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58192 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 42 0.0 3 0 0 21 21 16 556 0.0 3 0 408 102 46 17 56611 0.0 3 51807 3782 821 201 18 232 0.0 3 17 142 25 48 19 175 0.0 3 1 0 90 84 20 343 0.0 3 2 0 0 341 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 27 1 0.0 3 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 1 37 2 0.0 3 1 0 0 1 39 2 0.0 3 1 0 0 1 40 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 50 8 0.0 3 0 0 1 7 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 5 0.0 3 0 0 0 5 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 115 8 0.0 3 0 0 0 8 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 136 1 0.0 3 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 7 0.0 3 0 0 0 7 151 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 1 4 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 38 0.0 3 0 0 0 38 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 11 0.0 3 0 0 1 10 206 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 235 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 5 0.0 3 0 0 1 4 281 13 0.0 3 0 0 0 13 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.03 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 58,061 Reads with adapters: 270 (0.5%) Reads written (passing filters): 58,061 (100.0%) Total basepairs processed: 16,013,875 bp Total written (filtered): 16,013,027 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 270 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.4% C: 16.3% G: 20.0% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 236 907.2 0 236 4 30 226.8 0 30 5 4 56.7 0 4