This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.23 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 85,881 (3.9%) Reads that were too short: 38,103 (1.7%) Reads written (passing filters): 47,778 (2.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 10,791,177 bp (1.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 85881 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 535.2 0 652 7 9663 535.2 0 9663 8 7341 535.2 0 7341 9 358 535.2 0 358 10 102 535.2 0 102 11 96 535.2 0 96 12 91 535.2 0 91 13 83 535.2 0 83 14 77 535.2 0 77 15 79 535.2 0 79 16 81 535.2 0 81 17 68 535.2 0 68 18 89 535.2 0 89 19 78 535.2 0 78 20 67 535.2 0 67 21 58 535.2 0 58 22 80 535.2 0 80 23 85 535.2 0 85 24 86 535.2 0 86 25 103 535.2 0 103 26 61 535.2 0 61 27 64 535.2 0 64 28 98 535.2 0 98 29 80 535.2 0 80 30 88 535.2 0 88 31 65 535.2 0 65 32 71 535.2 0 71 33 69 535.2 0 69 34 81 535.2 0 81 35 79 535.2 0 79 36 94 535.2 0 94 37 75 535.2 0 75 38 77 535.2 0 77 39 86 535.2 0 86 40 88 535.2 0 88 41 93 535.2 0 93 42 109 535.2 0 109 43 102 535.2 0 102 44 74 535.2 0 74 45 80 535.2 0 80 46 67 535.2 0 67 47 97 535.2 0 97 48 83 535.2 0 83 49 92 535.2 0 92 50 60 535.2 0 60 51 107 535.2 0 107 52 84 535.2 0 84 53 153 535.2 0 153 54 163 535.2 0 163 55 127 535.2 0 127 56 99 535.2 0 99 57 87 535.2 0 87 58 116 535.2 0 116 59 119 535.2 0 119 60 92 535.2 0 92 61 78 535.2 0 78 62 164 535.2 0 164 63 180 535.2 0 180 64 2485 535.2 0 2485 65 621 535.2 0 621 66 919 535.2 0 919 67 70 535.2 0 70 68 127 535.2 0 127 69 162 535.2 0 162 70 193 535.2 0 193 71 79 535.2 0 79 72 68 535.2 0 68 73 59 535.2 0 59 74 90 535.2 0 90 75 100 535.2 0 100 76 93 535.2 0 93 77 77 535.2 0 77 78 61 535.2 0 61 79 86 535.2 0 86 80 90 535.2 0 90 81 83 535.2 0 83 82 73 535.2 0 73 83 81 535.2 0 81 84 91 535.2 0 91 85 73 535.2 0 73 86 84 535.2 0 84 87 56 535.2 0 56 88 87 535.2 0 87 89 141 535.2 0 141 90 126 535.2 0 126 91 135 535.2 0 135 92 340 535.2 0 340 93 235 535.2 0 235 94 230 535.2 0 230 95 74 535.2 0 74 96 67 535.2 0 67 97 60 535.2 0 60 98 64 535.2 0 64 99 98 535.2 0 98 100 130 535.2 0 130 101 146 535.2 0 146 102 73 535.2 0 73 103 71 535.2 0 71 104 108 535.2 0 108 105 109 535.2 0 109 106 79 535.2 0 79 107 63 535.2 0 63 108 107 535.2 0 107 109 75 535.2 0 75 110 78 535.2 0 78 111 119 535.2 0 119 112 92 535.2 0 92 113 60 535.2 0 60 114 74 535.2 0 74 115 94 535.2 0 94 116 93 535.2 0 93 117 123 535.2 0 123 118 106 535.2 0 106 119 81 535.2 0 81 120 90 535.2 0 90 121 81 535.2 0 81 122 98 535.2 0 98 123 135 535.2 0 135 124 110 535.2 0 110 125 104 535.2 0 104 126 101 535.2 0 101 127 95 535.2 0 95 128 123 535.2 0 123 129 162 535.2 0 162 130 235 535.2 0 235 131 400 535.2 0 400 132 236 535.2 0 236 133 197 535.2 0 197 134 120 535.2 0 120 135 141 535.2 0 141 136 183 535.2 0 183 137 196 535.2 0 196 138 119 535.2 0 119 139 106 535.2 0 106 140 106 535.2 0 106 141 98 535.2 0 98 142 125 535.2 0 125 143 110 535.2 0 110 144 86 535.2 0 86 145 124 535.2 0 124 146 105 535.2 0 105 147 222 535.2 0 222 148 172 535.2 0 172 149 189 535.2 0 189 150 144 535.2 0 144 151 92 535.2 0 92 152 164 535.2 0 164 153 165 535.2 0 165 154 147 535.2 0 147 155 291 535.2 0 291 156 625 535.2 0 625 157 546 535.2 0 546 158 352 535.2 0 352 159 128 535.2 0 128 160 168 535.2 0 168 161 126 535.2 0 126 162 198 535.2 0 198 163 211 535.2 0 211 164 118 535.2 0 118 165 86 535.2 0 86 166 155 535.2 0 155 167 257 535.2 0 257 168 341 535.2 0 341 169 214 535.2 0 214 170 95 535.2 0 95 171 166 535.2 0 166 172 144 535.2 0 144 173 147 535.2 0 147 174 114 535.2 0 114 175 599 535.2 0 599 176 637 535.2 0 637 177 674 535.2 0 674 178 229 535.2 0 229 179 140 535.2 0 140 180 117 535.2 0 117 181 130 535.2 0 130 182 146 535.2 0 146 183 172 535.2 0 172 184 133 535.2 0 133 185 130 535.2 0 130 186 177 535.2 0 177 187 202 535.2 0 202 188 320 535.2 0 320 189 196 535.2 0 196 190 208 535.2 0 208 191 110 535.2 0 110 192 107 535.2 0 107 193 115 535.2 0 115 194 106 535.2 0 106 195 165 535.2 0 165 196 185 535.2 0 185 197 192 535.2 0 192 198 163 535.2 0 163 199 106 535.2 0 106 200 146 535.2 0 146 201 212 535.2 0 212 202 236 535.2 0 236 203 230 535.2 0 230 204 169 535.2 0 169 205 144 535.2 0 144 206 93 535.2 0 93 207 127 535.2 0 127 208 165 535.2 0 165 209 151 535.2 0 151 210 243 535.2 0 243 211 176 535.2 0 176 212 160 535.2 0 160 213 158 535.2 0 158 214 143 535.2 0 143 215 254 535.2 0 254 216 343 535.2 0 343 217 477 535.2 0 477 218 494 535.2 0 494 219 224 535.2 0 224 220 289 535.2 0 289 221 583 535.2 0 583 222 1087 535.2 0 1087 223 1234 535.2 0 1234 224 557 535.2 0 557 225 245 535.2 0 245 226 246 535.2 0 246 227 201 535.2 0 201 228 231 535.2 0 231 229 182 535.2 0 182 230 135 535.2 0 135 231 120 535.2 0 120 232 188 535.2 0 188 233 170 535.2 0 170 234 187 535.2 0 187 235 159 535.2 0 159 236 183 535.2 0 183 237 228 535.2 0 228 238 216 535.2 0 216 239 261 535.2 0 261 240 205 535.2 0 205 241 711 535.2 0 711 242 1037 535.2 0 1037 243 979 535.2 0 979 244 486 535.2 0 486 245 350 535.2 0 350 246 268 535.2 0 268 247 208 535.2 0 208 248 191 535.2 0 191 249 277 535.2 0 277 250 309 535.2 0 309 251 230 535.2 0 230 252 398 535.2 0 398 253 268 535.2 0 268 254 589 535.2 0 589 255 315 535.2 0 315 256 363 535.2 0 363 257 282 535.2 0 282 258 160 535.2 0 160 259 122 535.2 0 122 260 149 535.2 0 149 261 205 535.2 0 205 262 227 535.2 0 227 263 362 535.2 0 362 264 410 535.2 0 410 265 315 535.2 0 315 266 259 535.2 0 259 267 250 535.2 0 250 268 1133 535.2 0 1133 269 2800 535.2 0 2800 270 2892 535.2 0 2892 271 769 535.2 0 769 272 727 535.2 0 727 273 414 535.2 0 414 274 277 535.2 0 277 275 282 535.2 0 282 276 220 535.2 0 220 277 219 535.2 0 219 278 177 535.2 0 177 279 161 535.2 0 161 280 182 535.2 0 182 281 197 535.2 0 197 282 282 535.2 0 282 283 308 535.2 0 308 284 355 535.2 0 355 285 265 535.2 0 265 286 236 535.2 0 236 287 275 535.2 0 275 288 138 535.2 0 138 289 218 535.2 0 218 290 374 535.2 0 374 291 716 535.2 0 716 292 683 535.2 0 683 293 391 535.2 0 391 294 236 535.2 0 236 295 149 535.2 0 149 296 194 535.2 0 194 297 235 535.2 0 235 298 367 535.2 0 367 299 585 535.2 0 585 300 499 535.2 0 499 301 533 535.2 0 533 Finished in 2.05 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 47,778 Reads with adapters: 10,010 (21.0%) Reads that were too short: 119 (0.2%) Reads written (passing filters): 9,891 (20.7%) Total basepairs processed: 10,791,177 bp Total written (filtered): 2,716,923 bp (25.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10010 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 3 6 16 72 0.0 3 0 61 6 5 17 7946 0.0 3 7324 502 97 23 18 37 0.0 3 2 26 7 2 19 1647 0.0 3 1456 129 36 26 20 57 0.0 3 22 4 2 29 21 5 0.0 3 0 0 1 4 22 4 0.0 3 0 0 0 4 23 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 0 3 29 5 0.0 3 0 0 0 5 31 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 12 0.0 3 0 0 1 11 37 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 39 3 0.0 3 0 0 1 2 41 15 0.0 3 0 0 0 15 42 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 45 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 8 0.0 3 0 0 0 8 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 1 1 66 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 1 2 89 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 3 0.0 3 0 0 0 3 94 3 0.0 3 0 0 0 3 95 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 100 2 0.0 3 0 0 1 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 1 111 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 1 2 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 9,891 Reads with adapters: 86 (0.9%) Reads written (passing filters): 9,891 (100.0%) Total basepairs processed: 2,716,923 bp Total written (filtered): 2,716,664 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 86 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.1% C: 20.9% G: 20.9% T: 29.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 85 154.5 0 85 4 1 38.6 0 1