This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.24 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 44,202 (2.0%) Reads that were too short: 13,100 (0.6%) Reads written (passing filters): 31,102 (1.4%) Total basepairs processed: 659,880,795 bp Total written (filtered): 7,802,059 bp (1.2%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 44202 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 531 535.2 0 531 7 8993 535.2 0 8993 8 8812 535.2 0 8812 9 124 535.2 0 124 10 51 535.2 0 51 11 42 535.2 0 42 12 48 535.2 0 48 13 52 535.2 0 52 14 46 535.2 0 46 15 43 535.2 0 43 16 45 535.2 0 45 17 49 535.2 0 49 18 50 535.2 0 50 19 40 535.2 0 40 20 48 535.2 0 48 21 43 535.2 0 43 22 39 535.2 0 39 23 41 535.2 0 41 24 38 535.2 0 38 25 43 535.2 0 43 26 39 535.2 0 39 27 41 535.2 0 41 28 66 535.2 0 66 29 57 535.2 0 57 30 55 535.2 0 55 31 49 535.2 0 49 32 134 535.2 0 134 33 63 535.2 0 63 34 39 535.2 0 39 35 50 535.2 0 50 36 38 535.2 0 38 37 52 535.2 0 52 38 56 535.2 0 56 39 50 535.2 0 50 40 50 535.2 0 50 41 48 535.2 0 48 42 47 535.2 0 47 43 53 535.2 0 53 44 62 535.2 0 62 45 39 535.2 0 39 46 48 535.2 0 48 47 74 535.2 0 74 48 43 535.2 0 43 49 54 535.2 0 54 50 39 535.2 0 39 51 39 535.2 0 39 52 41 535.2 0 41 53 53 535.2 0 53 54 72 535.2 0 72 55 53 535.2 0 53 56 28 535.2 0 28 57 44 535.2 0 44 58 40 535.2 0 40 59 48 535.2 0 48 60 35 535.2 0 35 61 30 535.2 0 30 62 50 535.2 0 50 63 76 535.2 0 76 64 570 535.2 0 570 65 132 535.2 0 132 66 218 535.2 0 218 67 28 535.2 0 28 68 83 535.2 0 83 69 87 535.2 0 87 70 49 535.2 0 49 71 31 535.2 0 31 72 38 535.2 0 38 73 34 535.2 0 34 74 40 535.2 0 40 75 36 535.2 0 36 76 43 535.2 0 43 77 33 535.2 0 33 78 32 535.2 0 32 79 43 535.2 0 43 80 55 535.2 0 55 81 64 535.2 0 64 82 30 535.2 0 30 83 52 535.2 0 52 84 49 535.2 0 49 85 49 535.2 0 49 86 50 535.2 0 50 87 37 535.2 0 37 88 37 535.2 0 37 89 70 535.2 0 70 90 73 535.2 0 73 91 74 535.2 0 74 92 238 535.2 0 238 93 155 535.2 0 155 94 117 535.2 0 117 95 46 535.2 0 46 96 38 535.2 0 38 97 35 535.2 0 35 98 33 535.2 0 33 99 41 535.2 0 41 100 44 535.2 0 44 101 43 535.2 0 43 102 49 535.2 0 49 103 50 535.2 0 50 104 57 535.2 0 57 105 56 535.2 0 56 106 57 535.2 0 57 107 39 535.2 0 39 108 67 535.2 0 67 109 48 535.2 0 48 110 46 535.2 0 46 111 64 535.2 0 64 112 49 535.2 0 49 113 40 535.2 0 40 114 45 535.2 0 45 115 56 535.2 0 56 116 58 535.2 0 58 117 61 535.2 0 61 118 71 535.2 0 71 119 41 535.2 0 41 120 70 535.2 0 70 121 60 535.2 0 60 122 65 535.2 0 65 123 61 535.2 0 61 124 49 535.2 0 49 125 52 535.2 0 52 126 61 535.2 0 61 127 48 535.2 0 48 128 47 535.2 0 47 129 64 535.2 0 64 130 72 535.2 0 72 131 88 535.2 0 88 132 85 535.2 0 85 133 64 535.2 0 64 134 39 535.2 0 39 135 53 535.2 0 53 136 58 535.2 0 58 137 70 535.2 0 70 138 57 535.2 0 57 139 67 535.2 0 67 140 73 535.2 0 73 141 86 535.2 0 86 142 86 535.2 0 86 143 50 535.2 0 50 144 42 535.2 0 42 145 45 535.2 0 45 146 46 535.2 0 46 147 76 535.2 0 76 148 55 535.2 0 55 149 89 535.2 0 89 150 96 535.2 0 96 151 58 535.2 0 58 152 66 535.2 0 66 153 103 535.2 0 103 154 62 535.2 0 62 155 107 535.2 0 107 156 160 535.2 0 160 157 133 535.2 0 133 158 108 535.2 0 108 159 52 535.2 0 52 160 65 535.2 0 65 161 62 535.2 0 62 162 65 535.2 0 65 163 65 535.2 0 65 164 48 535.2 0 48 165 44 535.2 0 44 166 66 535.2 0 66 167 79 535.2 0 79 168 85 535.2 0 85 169 74 535.2 0 74 170 48 535.2 0 48 171 74 535.2 0 74 172 73 535.2 0 73 173 77 535.2 0 77 174 54 535.2 0 54 175 220 535.2 0 220 176 194 535.2 0 194 177 191 535.2 0 191 178 77 535.2 0 77 179 62 535.2 0 62 180 71 535.2 0 71 181 62 535.2 0 62 182 48 535.2 0 48 183 73 535.2 0 73 184 74 535.2 0 74 185 52 535.2 0 52 186 97 535.2 0 97 187 63 535.2 0 63 188 100 535.2 0 100 189 87 535.2 0 87 190 97 535.2 0 97 191 70 535.2 0 70 192 70 535.2 0 70 193 68 535.2 0 68 194 52 535.2 0 52 195 50 535.2 0 50 196 72 535.2 0 72 197 65 535.2 0 65 198 63 535.2 0 63 199 78 535.2 0 78 200 75 535.2 0 75 201 55 535.2 0 55 202 64 535.2 0 64 203 80 535.2 0 80 204 74 535.2 0 74 205 81 535.2 0 81 206 78 535.2 0 78 207 71 535.2 0 71 208 80 535.2 0 80 209 65 535.2 0 65 210 72 535.2 0 72 211 77 535.2 0 77 212 82 535.2 0 82 213 88 535.2 0 88 214 73 535.2 0 73 215 92 535.2 0 92 216 93 535.2 0 93 217 119 535.2 0 119 218 110 535.2 0 110 219 94 535.2 0 94 220 85 535.2 0 85 221 138 535.2 0 138 222 193 535.2 0 193 223 191 535.2 0 191 224 156 535.2 0 156 225 108 535.2 0 108 226 132 535.2 0 132 227 97 535.2 0 97 228 105 535.2 0 105 229 86 535.2 0 86 230 77 535.2 0 77 231 55 535.2 0 55 232 65 535.2 0 65 233 65 535.2 0 65 234 117 535.2 0 117 235 79 535.2 0 79 236 73 535.2 0 73 237 102 535.2 0 102 238 72 535.2 0 72 239 90 535.2 0 90 240 82 535.2 0 82 241 222 535.2 0 222 242 219 535.2 0 219 243 241 535.2 0 241 244 143 535.2 0 143 245 90 535.2 0 90 246 98 535.2 0 98 247 100 535.2 0 100 248 95 535.2 0 95 249 90 535.2 0 90 250 96 535.2 0 96 251 96 535.2 0 96 252 131 535.2 0 131 253 131 535.2 0 131 254 169 535.2 0 169 255 98 535.2 0 98 256 94 535.2 0 94 257 90 535.2 0 90 258 73 535.2 0 73 259 68 535.2 0 68 260 66 535.2 0 66 261 60 535.2 0 60 262 99 535.2 0 99 263 106 535.2 0 106 264 100 535.2 0 100 265 96 535.2 0 96 266 107 535.2 0 107 267 100 535.2 0 100 268 469 535.2 0 469 269 1049 535.2 0 1049 270 1004 535.2 0 1004 271 167 535.2 0 167 272 148 535.2 0 148 273 154 535.2 0 154 274 95 535.2 0 95 275 114 535.2 0 114 276 111 535.2 0 111 277 98 535.2 0 98 278 104 535.2 0 104 279 77 535.2 0 77 280 83 535.2 0 83 281 75 535.2 0 75 282 83 535.2 0 83 283 107 535.2 0 107 284 125 535.2 0 125 285 92 535.2 0 92 286 83 535.2 0 83 287 105 535.2 0 105 288 96 535.2 0 96 289 78 535.2 0 78 290 119 535.2 0 119 291 159 535.2 0 159 292 193 535.2 0 193 293 127 535.2 0 127 294 87 535.2 0 87 295 95 535.2 0 95 296 95 535.2 0 95 297 123 535.2 0 123 298 184 535.2 0 184 299 224 535.2 0 224 300 216 535.2 0 216 301 222 535.2 0 222 Finished in 1.59 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 31,102 Reads with adapters: 9,248 (29.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 9,235 (29.7%) Total basepairs processed: 7,802,059 bp Total written (filtered): 2,516,487 bp (32.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 88 0.0 3 0 88 20 8879 0.0 4 8749 121 8 0 1 21 33 0.0 4 4 27 2 22 4 0.0 4 0 3 1 23 186 0.0 4 178 3 0 5 24 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 44 1 0.0 4 1 66 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 1 1 87 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 127 4 0.0 4 0 0 0 0 4 129 31 0.0 4 0 0 0 0 31 132 1 0.0 4 0 0 0 0 1 153 1 0.0 4 1 154 2 0.0 4 0 0 1 0 1 155 1 0.0 4 0 0 0 1 156 2 0.0 4 0 1 0 0 1 163 1 0.0 4 1 228 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 9,235 Reads with adapters: 1,115 (12.1%) Reads written (passing filters): 9,235 (100.0%) Total basepairs processed: 2,516,487 bp Total written (filtered): 2,504,280 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1115 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.5% G: 95.8% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 144.3 0 2 4 1 36.1 0 1 6 10 2.3 0 10 9 24 0.0 0 1 23 10 82 0.0 1 3 79 11 991 0.0 1 4 987 12 3 0.0 1 0 3 13 1 0.0 1 0 1 151 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.