This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.69 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 455,301 (20.8%) Reads that were too short: 304,683 (13.9%) Reads written (passing filters): 150,618 (6.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 41,932,271 bp (6.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 455301 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 890 535.2 0 890 7 74500 535.2 0 74500 8 1464 535.2 0 1464 9 54247 535.2 0 54247 10 113 535.2 0 113 11 64 535.2 0 64 12 51 535.2 0 51 13 69 535.2 0 69 14 78 535.2 0 78 15 70 535.2 0 70 16 67 535.2 0 67 17 58 535.2 0 58 18 50 535.2 0 50 19 64 535.2 0 64 20 46 535.2 0 46 21 58 535.2 0 58 22 56 535.2 0 56 23 72 535.2 0 72 24 39 535.2 0 39 25 61 535.2 0 61 26 63 535.2 0 63 27 66 535.2 0 66 28 61 535.2 0 61 29 44 535.2 0 44 30 63 535.2 0 63 31 63 535.2 0 63 32 66 535.2 0 66 33 60 535.2 0 60 34 43 535.2 0 43 35 57 535.2 0 57 36 158 535.2 0 158 37 97 535.2 0 97 38 159 535.2 0 159 39 45 535.2 0 45 40 61 535.2 0 61 41 56 535.2 0 56 42 53 535.2 0 53 43 62 535.2 0 62 44 47 535.2 0 47 45 54 535.2 0 54 46 57 535.2 0 57 47 34 535.2 0 34 48 34 535.2 0 34 49 45 535.2 0 45 50 43 535.2 0 43 51 40 535.2 0 40 52 46 535.2 0 46 53 37 535.2 0 37 54 39 535.2 0 39 55 47 535.2 0 47 56 38 535.2 0 38 57 36 535.2 0 36 58 45 535.2 0 45 59 62 535.2 0 62 60 63 535.2 0 63 61 51 535.2 0 51 62 38 535.2 0 38 63 40 535.2 0 40 64 29 535.2 0 29 65 46 535.2 0 46 66 75 535.2 0 75 67 56 535.2 0 56 68 63 535.2 0 63 69 117 535.2 0 117 70 113 535.2 0 113 71 144 535.2 0 144 72 56 535.2 0 56 73 112 535.2 0 112 74 54 535.2 0 54 75 66 535.2 0 66 76 83 535.2 0 83 77 65 535.2 0 65 78 107 535.2 0 107 79 86 535.2 0 86 80 103 535.2 0 103 81 86 535.2 0 86 82 94 535.2 0 94 83 109 535.2 0 109 84 91 535.2 0 91 85 97 535.2 0 97 86 109 535.2 0 109 87 66 535.2 0 66 88 105 535.2 0 105 89 72 535.2 0 72 90 78 535.2 0 78 91 68 535.2 0 68 92 92 535.2 0 92 93 77 535.2 0 77 94 69 535.2 0 69 95 75 535.2 0 75 96 96 535.2 0 96 97 96 535.2 0 96 98 93 535.2 0 93 99 102 535.2 0 102 100 77 535.2 0 77 101 88 535.2 0 88 102 71 535.2 0 71 103 92 535.2 0 92 104 101 535.2 0 101 105 85 535.2 0 85 106 105 535.2 0 105 107 95 535.2 0 95 108 85 535.2 0 85 109 100 535.2 0 100 110 96 535.2 0 96 111 116 535.2 0 116 112 110 535.2 0 110 113 73 535.2 0 73 114 94 535.2 0 94 115 85 535.2 0 85 116 66 535.2 0 66 117 70 535.2 0 70 118 72 535.2 0 72 119 169 535.2 0 169 120 146 535.2 0 146 121 128 535.2 0 128 122 164 535.2 0 164 123 84 535.2 0 84 124 175 535.2 0 175 125 113 535.2 0 113 126 75 535.2 0 75 127 103 535.2 0 103 128 76 535.2 0 76 129 92 535.2 0 92 130 72 535.2 0 72 131 92 535.2 0 92 132 96 535.2 0 96 133 117 535.2 0 117 134 87 535.2 0 87 135 83 535.2 0 83 136 81 535.2 0 81 137 89 535.2 0 89 138 82 535.2 0 82 139 328 535.2 0 328 140 174 535.2 0 174 141 164 535.2 0 164 142 108 535.2 0 108 143 73 535.2 0 73 144 71 535.2 0 71 145 97 535.2 0 97 146 180 535.2 0 180 147 138 535.2 0 138 148 117 535.2 0 117 149 83 535.2 0 83 150 67 535.2 0 67 151 52 535.2 0 52 152 62 535.2 0 62 153 80 535.2 0 80 154 103 535.2 0 103 155 127 535.2 0 127 156 114 535.2 0 114 157 99 535.2 0 99 158 110 535.2 0 110 159 866 535.2 0 866 160 1264 535.2 0 1264 161 1438 535.2 0 1438 162 81 535.2 0 81 163 70 535.2 0 70 164 79 535.2 0 79 165 88 535.2 0 88 166 91 535.2 0 91 167 94 535.2 0 94 168 121 535.2 0 121 169 72 535.2 0 72 170 84 535.2 0 84 171 58 535.2 0 58 172 115 535.2 0 115 173 118 535.2 0 118 174 108 535.2 0 108 175 104 535.2 0 104 176 88 535.2 0 88 177 78 535.2 0 78 178 70 535.2 0 70 179 76 535.2 0 76 180 90 535.2 0 90 181 103 535.2 0 103 182 69 535.2 0 69 183 59 535.2 0 59 184 86 535.2 0 86 185 103 535.2 0 103 186 90 535.2 0 90 187 90 535.2 0 90 188 77 535.2 0 77 189 62 535.2 0 62 190 64 535.2 0 64 191 83 535.2 0 83 192 73 535.2 0 73 193 94 535.2 0 94 194 122 535.2 0 122 195 98 535.2 0 98 196 93 535.2 0 93 197 134 535.2 0 134 198 104 535.2 0 104 199 112 535.2 0 112 200 76 535.2 0 76 201 81 535.2 0 81 202 73 535.2 0 73 203 121 535.2 0 121 204 93 535.2 0 93 205 122 535.2 0 122 206 240 535.2 0 240 207 162 535.2 0 162 208 169 535.2 0 169 209 213 535.2 0 213 210 258 535.2 0 258 211 157 535.2 0 157 212 193 535.2 0 193 213 242 535.2 0 242 214 213 535.2 0 213 215 114 535.2 0 114 216 125 535.2 0 125 217 127 535.2 0 127 218 339 535.2 0 339 219 149 535.2 0 149 220 144 535.2 0 144 221 208 535.2 0 208 222 126 535.2 0 126 223 164 535.2 0 164 224 148 535.2 0 148 225 234 535.2 0 234 226 118 535.2 0 118 227 191 535.2 0 191 228 744 535.2 0 744 229 160 535.2 0 160 230 124 535.2 0 124 231 124 535.2 0 124 232 191 535.2 0 191 233 167 535.2 0 167 234 181 535.2 0 181 235 212 535.2 0 212 236 178 535.2 0 178 237 418 535.2 0 418 238 1006 535.2 0 1006 239 231 535.2 0 231 240 400 535.2 0 400 241 267 535.2 0 267 242 323 535.2 0 323 243 632 535.2 0 632 244 434 535.2 0 434 245 435 535.2 0 435 246 204 535.2 0 204 247 331 535.2 0 331 248 649 535.2 0 649 249 685 535.2 0 685 250 1246 535.2 0 1246 251 1444 535.2 0 1444 252 2058 535.2 0 2058 253 4317 535.2 0 4317 254 12860 535.2 0 12860 255 82699 535.2 0 82699 256 81382 535.2 0 81382 257 100724 535.2 0 100724 258 1912 535.2 0 1912 259 122 535.2 0 122 260 135 535.2 0 135 261 97 535.2 0 97 262 148 535.2 0 148 263 99 535.2 0 99 264 79 535.2 0 79 265 79 535.2 0 79 266 119 535.2 0 119 267 140 535.2 0 140 268 114 535.2 0 114 269 59 535.2 0 59 270 62 535.2 0 62 271 66 535.2 0 66 272 70 535.2 0 70 273 74 535.2 0 74 274 84 535.2 0 84 275 62 535.2 0 62 276 97 535.2 0 97 277 78 535.2 0 78 278 64 535.2 0 64 279 93 535.2 0 93 280 57 535.2 0 57 281 55 535.2 0 55 282 70 535.2 0 70 283 76 535.2 0 76 284 85 535.2 0 85 285 72 535.2 0 72 286 62 535.2 0 62 287 49 535.2 0 49 288 73 535.2 0 73 289 61 535.2 0 61 290 60 535.2 0 60 291 70 535.2 0 70 292 62 535.2 0 62 293 50 535.2 0 50 294 75 535.2 0 75 295 85 535.2 0 85 296 70 535.2 0 70 297 55 535.2 0 55 298 66 535.2 0 66 299 117 535.2 0 117 300 85 535.2 0 85 301 106 535.2 0 106 Finished in 7.33 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 150,618 Reads with adapters: 75,234 (50.0%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 75,215 (49.9%) Total basepairs processed: 41,932,271 bp Total written (filtered): 20,556,469 bp (49.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 75234 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 13 19 445 0.0 3 0 435 10 20 74039 0.0 4 72947 1047 42 2 1 21 171 0.0 4 24 146 1 22 12 0.0 4 3 0 9 23 42 0.0 4 3 0 0 36 3 24 3 0.0 4 1 0 0 0 2 32 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 7 0.0 4 0 0 0 0 7 99 1 0.0 4 1 104 7 0.0 4 0 0 0 0 7 117 1 0.0 4 1 122 1 0.0 4 1 123 2 0.0 4 2 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 458 0.0 4 0 0 0 0 458 136 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 1 263 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.59 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 75,215 Reads with adapters: 1,714 (2.3%) Reads written (passing filters): 75,215 (100.0%) Total basepairs processed: 20,556,469 bp Total written (filtered): 20,460,557 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1714 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.5% C: 23.6% G: 67.3% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 120 1175.2 0 120 4 7 293.8 0 7 5 5 73.5 0 5 7 1 4.6 0 1 9 3 0.3 0 2 1 10 11 0.1 1 1 10 11 1073 0.0 1 8 1065 12 109 0.0 1 2 107 13 5 0.0 1 0 5 14 1 0.0 1 0 1 15 2 0.0 1 0 2 21 1 0.0 1 0 1 24 1 0.0 1 1 149 2 0.0 1 2 152 24 0.0 1 0 24 153 1 0.0 1 0 1 161 1 0.0 1 0 1 163 1 0.0 1 1 210 13 0.0 1 6 7 225 328 0.0 1 297 31 226 3 0.0 1 3 229 1 0.0 1 1 230 1 0.0 1 0 1