This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.05 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 108,052 (4.9%) Reads that were too short: 37,060 (1.7%) Reads written (passing filters): 70,992 (3.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 14,776,158 bp (2.2%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 108052 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 948 535.2 0 948 7 616 535.2 0 616 8 15639 535.2 0 15639 9 13088 535.2 0 13088 10 41 535.2 0 41 11 44 535.2 0 44 12 40 535.2 0 40 13 33 535.2 0 33 14 38 535.2 0 38 15 42 535.2 0 42 16 43 535.2 0 43 17 49 535.2 0 49 18 48 535.2 0 48 19 48 535.2 0 48 20 46 535.2 0 46 21 86 535.2 0 86 22 68 535.2 0 68 23 79 535.2 0 79 24 58 535.2 0 58 25 39 535.2 0 39 26 99 535.2 0 99 27 398 535.2 0 398 28 46 535.2 0 46 29 98 535.2 0 98 30 42 535.2 0 42 31 40 535.2 0 40 32 43 535.2 0 43 33 63 535.2 0 63 34 66 535.2 0 66 35 45 535.2 0 45 36 44 535.2 0 44 37 44 535.2 0 44 38 76 535.2 0 76 39 42 535.2 0 42 40 25 535.2 0 25 41 56 535.2 0 56 42 45 535.2 0 45 43 49 535.2 0 49 44 47 535.2 0 47 45 55 535.2 0 55 46 47 535.2 0 47 47 58 535.2 0 58 48 50 535.2 0 50 49 34 535.2 0 34 50 52 535.2 0 52 51 85 535.2 0 85 52 61 535.2 0 61 53 51 535.2 0 51 54 43 535.2 0 43 55 38 535.2 0 38 56 32 535.2 0 32 57 47 535.2 0 47 58 41 535.2 0 41 59 48 535.2 0 48 60 43 535.2 0 43 61 38 535.2 0 38 62 24 535.2 0 24 63 45 535.2 0 45 64 55 535.2 0 55 65 68 535.2 0 68 66 70 535.2 0 70 67 36 535.2 0 36 68 59 535.2 0 59 69 86 535.2 0 86 70 186 535.2 0 186 71 57 535.2 0 57 72 55 535.2 0 55 73 42 535.2 0 42 74 48 535.2 0 48 75 61 535.2 0 61 76 92 535.2 0 92 77 66 535.2 0 66 78 59 535.2 0 59 79 240 535.2 0 240 80 190 535.2 0 190 81 254 535.2 0 254 82 69 535.2 0 69 83 58 535.2 0 58 84 55 535.2 0 55 85 75 535.2 0 75 86 45 535.2 0 45 87 43 535.2 0 43 88 41 535.2 0 41 89 49 535.2 0 49 90 39 535.2 0 39 91 38 535.2 0 38 92 40 535.2 0 40 93 53 535.2 0 53 94 41 535.2 0 41 95 40 535.2 0 40 96 71 535.2 0 71 97 52 535.2 0 52 98 58 535.2 0 58 99 86 535.2 0 86 100 154 535.2 0 154 101 140 535.2 0 140 102 71 535.2 0 71 103 41 535.2 0 41 104 38 535.2 0 38 105 41 535.2 0 41 106 46 535.2 0 46 107 100 535.2 0 100 108 51 535.2 0 51 109 102 535.2 0 102 110 42 535.2 0 42 111 70 535.2 0 70 112 84 535.2 0 84 113 168 535.2 0 168 114 134 535.2 0 134 115 62 535.2 0 62 116 72 535.2 0 72 117 308 535.2 0 308 118 385 535.2 0 385 119 300 535.2 0 300 120 68 535.2 0 68 121 66 535.2 0 66 122 79 535.2 0 79 123 72 535.2 0 72 124 55 535.2 0 55 125 56 535.2 0 56 126 105 535.2 0 105 127 65 535.2 0 65 128 126 535.2 0 126 129 60 535.2 0 60 130 92 535.2 0 92 131 239 535.2 0 239 132 328 535.2 0 328 133 300 535.2 0 300 134 97 535.2 0 97 135 111 535.2 0 111 136 94 535.2 0 94 137 88 535.2 0 88 138 175 535.2 0 175 139 136 535.2 0 136 140 190 535.2 0 190 141 55 535.2 0 55 142 45 535.2 0 45 143 52 535.2 0 52 144 55 535.2 0 55 145 77 535.2 0 77 146 87 535.2 0 87 147 98 535.2 0 98 148 85 535.2 0 85 149 54 535.2 0 54 150 50 535.2 0 50 151 60 535.2 0 60 152 47 535.2 0 47 153 57 535.2 0 57 154 72 535.2 0 72 155 86 535.2 0 86 156 332 535.2 0 332 157 261 535.2 0 261 158 320 535.2 0 320 159 68 535.2 0 68 160 69 535.2 0 69 161 93 535.2 0 93 162 74 535.2 0 74 163 79 535.2 0 79 164 77 535.2 0 77 165 114 535.2 0 114 166 70 535.2 0 70 167 92 535.2 0 92 168 137 535.2 0 137 169 119 535.2 0 119 170 87 535.2 0 87 171 155 535.2 0 155 172 114 535.2 0 114 173 426 535.2 0 426 174 236 535.2 0 236 175 769 535.2 0 769 176 466 535.2 0 466 177 1074 535.2 0 1074 178 5103 535.2 0 5103 179 3003 535.2 0 3003 180 4873 535.2 0 4873 181 969 535.2 0 969 182 1876 535.2 0 1876 183 1369 535.2 0 1369 184 402 535.2 0 402 185 151 535.2 0 151 186 93 535.2 0 93 187 86 535.2 0 86 188 155 535.2 0 155 189 109 535.2 0 109 190 69 535.2 0 69 191 74 535.2 0 74 192 81 535.2 0 81 193 93 535.2 0 93 194 134 535.2 0 134 195 269 535.2 0 269 196 1123 535.2 0 1123 197 1356 535.2 0 1356 198 768 535.2 0 768 199 367 535.2 0 367 200 320 535.2 0 320 201 896 535.2 0 896 202 1987 535.2 0 1987 203 1683 535.2 0 1683 204 539 535.2 0 539 205 42 535.2 0 42 206 58 535.2 0 58 207 46 535.2 0 46 208 49 535.2 0 49 209 79 535.2 0 79 210 76 535.2 0 76 211 55 535.2 0 55 212 65 535.2 0 65 213 65 535.2 0 65 214 63 535.2 0 63 215 125 535.2 0 125 216 160 535.2 0 160 217 182 535.2 0 182 218 112 535.2 0 112 219 112 535.2 0 112 220 104 535.2 0 104 221 131 535.2 0 131 222 391 535.2 0 391 223 705 535.2 0 705 224 698 535.2 0 698 225 318 535.2 0 318 226 69 535.2 0 69 227 72 535.2 0 72 228 110 535.2 0 110 229 67 535.2 0 67 230 75 535.2 0 75 231 80 535.2 0 80 232 87 535.2 0 87 233 132 535.2 0 132 234 680 535.2 0 680 235 853 535.2 0 853 236 1341 535.2 0 1341 237 116 535.2 0 116 238 142 535.2 0 142 239 435 535.2 0 435 240 899 535.2 0 899 241 841 535.2 0 841 242 323 535.2 0 323 243 540 535.2 0 540 244 320 535.2 0 320 245 166 535.2 0 166 246 95 535.2 0 95 247 66 535.2 0 66 248 64 535.2 0 64 249 68 535.2 0 68 250 111 535.2 0 111 251 118 535.2 0 118 252 189 535.2 0 189 253 179 535.2 0 179 254 176 535.2 0 176 255 119 535.2 0 119 256 139 535.2 0 139 257 324 535.2 0 324 258 490 535.2 0 490 259 1304 535.2 0 1304 260 794 535.2 0 794 261 996 535.2 0 996 262 194 535.2 0 194 263 429 535.2 0 429 264 1635 535.2 0 1635 265 3614 535.2 0 3614 266 2538 535.2 0 2538 267 638 535.2 0 638 268 55 535.2 0 55 269 71 535.2 0 71 270 89 535.2 0 89 271 72 535.2 0 72 272 66 535.2 0 66 273 67 535.2 0 67 274 135 535.2 0 135 275 227 535.2 0 227 276 272 535.2 0 272 277 189 535.2 0 189 278 230 535.2 0 230 279 139 535.2 0 139 280 62 535.2 0 62 281 53 535.2 0 53 282 54 535.2 0 54 283 56 535.2 0 56 284 77 535.2 0 77 285 81 535.2 0 81 286 83 535.2 0 83 287 108 535.2 0 108 288 83 535.2 0 83 289 88 535.2 0 88 290 131 535.2 0 131 291 1107 535.2 0 1107 292 1310 535.2 0 1310 293 1378 535.2 0 1378 294 221 535.2 0 221 295 92 535.2 0 92 296 115 535.2 0 115 297 146 535.2 0 146 298 128 535.2 0 128 299 208 535.2 0 208 300 208 535.2 0 208 301 186 535.2 0 186 Finished in 2.88 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 70,992 Reads with adapters: 16,386 (23.1%) Reads that were too short: 203 (0.3%) Reads written (passing filters): 16,183 (22.8%) Total basepairs processed: 14,776,158 bp Total written (filtered): 4,439,051 bp (30.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16386 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 6 3 16 205 0.0 3 0 148 23 34 17 14939 0.0 3 13517 1111 243 68 18 66 0.0 3 5 43 14 4 19 87 0.0 3 8 15 39 25 20 703 0.0 3 597 47 12 47 21 4 0.0 3 0 2 1 1 22 6 0.0 3 0 0 5 1 23 3 0.0 3 1 0 0 2 24 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 10 0.0 3 0 0 1 9 28 2 0.0 3 0 0 1 1 29 1 0.0 3 0 1 30 3 0.0 3 0 0 0 3 31 2 0.0 3 0 0 0 2 33 3 0.0 3 0 0 0 3 35 10 0.0 3 0 0 0 10 38 1 0.0 3 0 0 0 1 41 13 0.0 3 0 0 2 11 42 1 0.0 3 0 0 0 1 43 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 1 48 2 0.0 3 0 0 1 1 49 2 0.0 3 0 0 0 2 50 7 0.0 3 0 0 0 7 54 11 0.0 3 0 0 1 10 56 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 24 0.0 3 0 0 0 24 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 8 0.0 3 0 0 0 8 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 20 0.0 3 0 0 0 20 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 8 0.0 3 0 0 0 8 81 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 0 2 83 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 10 0.0 3 0 0 0 10 92 17 0.0 3 0 0 0 17 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 36 0.0 3 0 0 0 36 103 2 0.0 3 0 0 0 2 104 6 0.0 3 0 0 0 6 105 9 0.0 3 0 0 0 9 106 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 0 4 111 4 0.0 3 0 0 1 3 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 140 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 1 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 1 209 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 1 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 1 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 0 4 295 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 16,183 Reads with adapters: 141 (0.9%) Reads written (passing filters): 16,183 (100.0%) Total basepairs processed: 4,439,051 bp Total written (filtered): 4,438,594 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 141 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.7% C: 14.2% G: 25.5% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 120 252.9 0 120 4 16 63.2 0 16 5 4 15.8 0 4 13 1 0.0 1 0 1