This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.73 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 82,903 (3.8%) Reads that were too short: 22,898 (1.0%) Reads written (passing filters): 60,005 (2.7%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,345,039 bp (2.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 82903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 896 535.2 0 896 7 461 535.2 0 461 8 14794 535.2 0 14794 9 15824 535.2 0 15824 10 47 535.2 0 47 11 28 535.2 0 28 12 12 535.2 0 12 13 40 535.2 0 40 14 20 535.2 0 20 15 24 535.2 0 24 16 19 535.2 0 19 17 24 535.2 0 24 18 36 535.2 0 36 19 21 535.2 0 21 20 25 535.2 0 25 21 45 535.2 0 45 22 52 535.2 0 52 23 36 535.2 0 36 24 22 535.2 0 22 25 23 535.2 0 23 26 42 535.2 0 42 27 37 535.2 0 37 28 32 535.2 0 32 29 51 535.2 0 51 30 25 535.2 0 25 31 22 535.2 0 22 32 19 535.2 0 19 33 50 535.2 0 50 34 42 535.2 0 42 35 46 535.2 0 46 36 28 535.2 0 28 37 32 535.2 0 32 38 39 535.2 0 39 39 45 535.2 0 45 40 38 535.2 0 38 41 32 535.2 0 32 42 44 535.2 0 44 43 32 535.2 0 32 44 36 535.2 0 36 45 28 535.2 0 28 46 23 535.2 0 23 47 43 535.2 0 43 48 45 535.2 0 45 49 36 535.2 0 36 50 42 535.2 0 42 51 27 535.2 0 27 52 39 535.2 0 39 53 35 535.2 0 35 54 40 535.2 0 40 55 29 535.2 0 29 56 33 535.2 0 33 57 44 535.2 0 44 58 39 535.2 0 39 59 38 535.2 0 38 60 38 535.2 0 38 61 28 535.2 0 28 62 35 535.2 0 35 63 23 535.2 0 23 64 32 535.2 0 32 65 38 535.2 0 38 66 34 535.2 0 34 67 29 535.2 0 29 68 32 535.2 0 32 69 76 535.2 0 76 70 184 535.2 0 184 71 48 535.2 0 48 72 26 535.2 0 26 73 24 535.2 0 24 74 34 535.2 0 34 75 23 535.2 0 23 76 40 535.2 0 40 77 43 535.2 0 43 78 51 535.2 0 51 79 254 535.2 0 254 80 119 535.2 0 119 81 174 535.2 0 174 82 20 535.2 0 20 83 35 535.2 0 35 84 27 535.2 0 27 85 59 535.2 0 59 86 29 535.2 0 29 87 24 535.2 0 24 88 27 535.2 0 27 89 24 535.2 0 24 90 26 535.2 0 26 91 21 535.2 0 21 92 18 535.2 0 18 93 35 535.2 0 35 94 25 535.2 0 25 95 33 535.2 0 33 96 44 535.2 0 44 97 31 535.2 0 31 98 38 535.2 0 38 99 31 535.2 0 31 100 59 535.2 0 59 101 47 535.2 0 47 102 24 535.2 0 24 103 28 535.2 0 28 104 30 535.2 0 30 105 26 535.2 0 26 106 34 535.2 0 34 107 70 535.2 0 70 108 33 535.2 0 33 109 101 535.2 0 101 110 40 535.2 0 40 111 40 535.2 0 40 112 40 535.2 0 40 113 59 535.2 0 59 114 33 535.2 0 33 115 26 535.2 0 26 116 42 535.2 0 42 117 109 535.2 0 109 118 89 535.2 0 89 119 87 535.2 0 87 120 40 535.2 0 40 121 26 535.2 0 26 122 30 535.2 0 30 123 32 535.2 0 32 124 40 535.2 0 40 125 35 535.2 0 35 126 71 535.2 0 71 127 33 535.2 0 33 128 73 535.2 0 73 129 36 535.2 0 36 130 25 535.2 0 25 131 74 535.2 0 74 132 94 535.2 0 94 133 60 535.2 0 60 134 48 535.2 0 48 135 38 535.2 0 38 136 40 535.2 0 40 137 60 535.2 0 60 138 76 535.2 0 76 139 49 535.2 0 49 140 155 535.2 0 155 141 29 535.2 0 29 142 24 535.2 0 24 143 39 535.2 0 39 144 34 535.2 0 34 145 33 535.2 0 33 146 52 535.2 0 52 147 46 535.2 0 46 148 40 535.2 0 40 149 38 535.2 0 38 150 34 535.2 0 34 151 24 535.2 0 24 152 33 535.2 0 33 153 47 535.2 0 47 154 53 535.2 0 53 155 62 535.2 0 62 156 317 535.2 0 317 157 265 535.2 0 265 158 291 535.2 0 291 159 38 535.2 0 38 160 43 535.2 0 43 161 49 535.2 0 49 162 59 535.2 0 59 163 45 535.2 0 45 164 61 535.2 0 61 165 97 535.2 0 97 166 68 535.2 0 68 167 55 535.2 0 55 168 89 535.2 0 89 169 71 535.2 0 71 170 73 535.2 0 73 171 132 535.2 0 132 172 91 535.2 0 91 173 397 535.2 0 397 174 170 535.2 0 170 175 749 535.2 0 749 176 280 535.2 0 280 177 642 535.2 0 642 178 4870 535.2 0 4870 179 2794 535.2 0 2794 180 5082 535.2 0 5082 181 563 535.2 0 563 182 1223 535.2 0 1223 183 317 535.2 0 317 184 226 535.2 0 226 185 85 535.2 0 85 186 56 535.2 0 56 187 52 535.2 0 52 188 119 535.2 0 119 189 41 535.2 0 41 190 38 535.2 0 38 191 33 535.2 0 33 192 43 535.2 0 43 193 49 535.2 0 49 194 73 535.2 0 73 195 106 535.2 0 106 196 431 535.2 0 431 197 474 535.2 0 474 198 318 535.2 0 318 199 114 535.2 0 114 200 224 535.2 0 224 201 238 535.2 0 238 202 571 535.2 0 571 203 496 535.2 0 496 204 167 535.2 0 167 205 32 535.2 0 32 206 38 535.2 0 38 207 30 535.2 0 30 208 41 535.2 0 41 209 38 535.2 0 38 210 49 535.2 0 49 211 42 535.2 0 42 212 42 535.2 0 42 213 36 535.2 0 36 214 31 535.2 0 31 215 76 535.2 0 76 216 74 535.2 0 74 217 92 535.2 0 92 218 46 535.2 0 46 219 49 535.2 0 49 220 55 535.2 0 55 221 83 535.2 0 83 222 149 535.2 0 149 223 217 535.2 0 217 224 263 535.2 0 263 225 153 535.2 0 153 226 45 535.2 0 45 227 48 535.2 0 48 228 51 535.2 0 51 229 56 535.2 0 56 230 52 535.2 0 52 231 57 535.2 0 57 232 70 535.2 0 70 233 109 535.2 0 109 234 876 535.2 0 876 235 1065 535.2 0 1065 236 1568 535.2 0 1568 237 78 535.2 0 78 238 96 535.2 0 96 239 257 535.2 0 257 240 672 535.2 0 672 241 521 535.2 0 521 242 124 535.2 0 124 243 147 535.2 0 147 244 124 535.2 0 124 245 72 535.2 0 72 246 50 535.2 0 50 247 46 535.2 0 46 248 29 535.2 0 29 249 61 535.2 0 61 250 68 535.2 0 68 251 74 535.2 0 74 252 97 535.2 0 97 253 84 535.2 0 84 254 78 535.2 0 78 255 71 535.2 0 71 256 73 535.2 0 73 257 111 535.2 0 111 258 193 535.2 0 193 259 1074 535.2 0 1074 260 631 535.2 0 631 261 931 535.2 0 931 262 129 535.2 0 129 263 420 535.2 0 420 264 1103 535.2 0 1103 265 2477 535.2 0 2477 266 1371 535.2 0 1371 267 402 535.2 0 402 268 35 535.2 0 35 269 31 535.2 0 31 270 28 535.2 0 28 271 34 535.2 0 34 272 34 535.2 0 34 273 57 535.2 0 57 274 81 535.2 0 81 275 62 535.2 0 62 276 79 535.2 0 79 277 52 535.2 0 52 278 83 535.2 0 83 279 65 535.2 0 65 280 33 535.2 0 33 281 45 535.2 0 45 282 51 535.2 0 51 283 50 535.2 0 50 284 50 535.2 0 50 285 50 535.2 0 50 286 52 535.2 0 52 287 61 535.2 0 61 288 45 535.2 0 45 289 56 535.2 0 56 290 79 535.2 0 79 291 690 535.2 0 690 292 858 535.2 0 858 293 731 535.2 0 731 294 124 535.2 0 124 295 79 535.2 0 79 296 75 535.2 0 75 297 128 535.2 0 128 298 92 535.2 0 92 299 122 535.2 0 122 300 144 535.2 0 144 301 111 535.2 0 111 Finished in 2.77 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 60,005 Reads with adapters: 16,789 (28.0%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 16,778 (28.0%) Total basepairs processed: 13,345,039 bp Total written (filtered): 4,550,730 bp (34.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16789 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 146 0.0 3 0 142 3 0 1 20 16428 0.0 4 16158 265 4 1 21 45 0.0 4 10 35 22 5 0.0 4 0 0 5 23 13 0.0 4 1 0 0 11 1 25 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 1 39 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 126 0.0 4 0 0 0 0 126 129 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 212 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 16,778 Reads with adapters: 2,256 (13.4%) Reads written (passing filters): 16,778 (100.0%) Total basepairs processed: 4,550,730 bp Total written (filtered): 4,528,068 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2256 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 3.5% G: 94.0% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 262.2 0 4 4 4 65.5 0 4 9 926 0.1 0 30 896 10 1073 0.0 1 46 1027 11 242 0.0 1 6 236 13 2 0.0 1 0 2 144 1 0.0 1 1 150 1 0.0 1 0 1 169 1 0.0 1 1 208 1 0.0 1 1 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.