This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.23 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 199,459 (9.1%) Reads that were too short: 118,120 (5.4%) Reads written (passing filters): 81,339 (3.7%) Total basepairs processed: 659,880,795 bp Total written (filtered): 20,245,390 bp (3.1%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 199459 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1136 535.2 0 1136 7 21954 535.2 0 21954 8 16920 535.2 0 16920 9 56 535.2 0 56 10 112 535.2 0 112 11 26 535.2 0 26 12 30 535.2 0 30 13 32 535.2 0 32 14 27 535.2 0 27 15 22 535.2 0 22 16 26 535.2 0 26 17 51 535.2 0 51 18 22 535.2 0 22 19 45 535.2 0 45 20 62 535.2 0 62 21 99 535.2 0 99 22 19 535.2 0 19 23 16 535.2 0 16 24 33 535.2 0 33 25 34 535.2 0 34 26 22 535.2 0 22 27 13 535.2 0 13 28 33 535.2 0 33 29 69 535.2 0 69 30 80 535.2 0 80 31 33 535.2 0 33 32 16 535.2 0 16 33 37 535.2 0 37 34 19 535.2 0 19 35 28 535.2 0 28 36 23 535.2 0 23 37 15 535.2 0 15 38 29 535.2 0 29 39 48 535.2 0 48 40 47 535.2 0 47 41 20 535.2 0 20 42 22 535.2 0 22 43 20 535.2 0 20 44 32 535.2 0 32 45 28 535.2 0 28 46 27 535.2 0 27 47 54 535.2 0 54 48 60 535.2 0 60 49 43 535.2 0 43 50 56 535.2 0 56 51 83 535.2 0 83 52 95 535.2 0 95 53 29 535.2 0 29 54 95 535.2 0 95 55 84 535.2 0 84 56 97 535.2 0 97 57 39 535.2 0 39 58 51 535.2 0 51 59 61 535.2 0 61 60 91 535.2 0 91 61 114 535.2 0 114 62 226 535.2 0 226 63 80 535.2 0 80 64 141 535.2 0 141 65 193 535.2 0 193 66 98 535.2 0 98 67 60 535.2 0 60 68 166 535.2 0 166 69 192 535.2 0 192 70 199 535.2 0 199 71 165 535.2 0 165 72 225 535.2 0 225 73 368 535.2 0 368 74 517 535.2 0 517 75 7081 535.2 0 7081 76 10513 535.2 0 10513 77 8426 535.2 0 8426 78 98 535.2 0 98 79 81 535.2 0 81 80 48 535.2 0 48 81 49 535.2 0 49 82 26 535.2 0 26 83 22 535.2 0 22 84 31 535.2 0 31 85 37 535.2 0 37 86 21 535.2 0 21 87 20 535.2 0 20 88 40 535.2 0 40 89 33 535.2 0 33 90 44 535.2 0 44 91 53 535.2 0 53 92 42 535.2 0 42 93 21 535.2 0 21 94 27 535.2 0 27 95 57 535.2 0 57 96 34 535.2 0 34 97 30 535.2 0 30 98 44 535.2 0 44 99 30 535.2 0 30 100 22 535.2 0 22 101 29 535.2 0 29 102 30 535.2 0 30 103 25 535.2 0 25 104 34 535.2 0 34 105 33 535.2 0 33 106 25 535.2 0 25 107 25 535.2 0 25 108 24 535.2 0 24 109 26 535.2 0 26 110 33 535.2 0 33 111 15 535.2 0 15 112 14 535.2 0 14 113 36 535.2 0 36 114 34 535.2 0 34 115 34 535.2 0 34 116 32 535.2 0 32 117 49 535.2 0 49 118 24 535.2 0 24 119 38 535.2 0 38 120 30 535.2 0 30 121 58 535.2 0 58 122 36 535.2 0 36 123 26 535.2 0 26 124 31 535.2 0 31 125 39 535.2 0 39 126 90 535.2 0 90 127 122 535.2 0 122 128 76 535.2 0 76 129 52 535.2 0 52 130 57 535.2 0 57 131 63 535.2 0 63 132 65 535.2 0 65 133 45 535.2 0 45 134 47 535.2 0 47 135 70 535.2 0 70 136 85 535.2 0 85 137 134 535.2 0 134 138 31 535.2 0 31 139 35 535.2 0 35 140 29 535.2 0 29 141 49 535.2 0 49 142 76 535.2 0 76 143 57 535.2 0 57 144 50 535.2 0 50 145 30 535.2 0 30 146 17 535.2 0 17 147 19 535.2 0 19 148 36 535.2 0 36 149 29 535.2 0 29 150 42 535.2 0 42 151 28 535.2 0 28 152 21 535.2 0 21 153 27 535.2 0 27 154 31 535.2 0 31 155 40 535.2 0 40 156 76 535.2 0 76 157 44 535.2 0 44 158 48 535.2 0 48 159 32 535.2 0 32 160 32 535.2 0 32 161 88 535.2 0 88 162 114 535.2 0 114 163 189 535.2 0 189 164 60 535.2 0 60 165 57 535.2 0 57 166 122 535.2 0 122 167 214 535.2 0 214 168 269 535.2 0 269 169 95 535.2 0 95 170 48 535.2 0 48 171 44 535.2 0 44 172 28 535.2 0 28 173 38 535.2 0 38 174 26 535.2 0 26 175 39 535.2 0 39 176 39 535.2 0 39 177 46 535.2 0 46 178 42 535.2 0 42 179 68 535.2 0 68 180 83 535.2 0 83 181 79 535.2 0 79 182 107 535.2 0 107 183 77 535.2 0 77 184 82 535.2 0 82 185 55 535.2 0 55 186 191 535.2 0 191 187 254 535.2 0 254 188 312 535.2 0 312 189 146 535.2 0 146 190 98 535.2 0 98 191 80 535.2 0 80 192 114 535.2 0 114 193 357 535.2 0 357 194 489 535.2 0 489 195 520 535.2 0 520 196 223 535.2 0 223 197 407 535.2 0 407 198 637 535.2 0 637 199 576 535.2 0 576 200 154 535.2 0 154 201 112 535.2 0 112 202 70 535.2 0 70 203 221 535.2 0 221 204 208 535.2 0 208 205 337 535.2 0 337 206 85 535.2 0 85 207 55 535.2 0 55 208 57 535.2 0 57 209 85 535.2 0 85 210 98 535.2 0 98 211 140 535.2 0 140 212 124 535.2 0 124 213 193 535.2 0 193 214 382 535.2 0 382 215 372 535.2 0 372 216 161 535.2 0 161 217 350 535.2 0 350 218 603 535.2 0 603 219 569 535.2 0 569 220 193 535.2 0 193 221 124 535.2 0 124 222 112 535.2 0 112 223 381 535.2 0 381 224 348 535.2 0 348 225 390 535.2 0 390 226 137 535.2 0 137 227 158 535.2 0 158 228 179 535.2 0 179 229 161 535.2 0 161 230 125 535.2 0 125 231 149 535.2 0 149 232 196 535.2 0 196 233 281 535.2 0 281 234 475 535.2 0 475 235 659 535.2 0 659 236 788 535.2 0 788 237 1090 535.2 0 1090 238 12033 535.2 0 12033 239 6383 535.2 0 6383 240 12205 535.2 0 12205 241 362 535.2 0 362 242 312 535.2 0 312 243 393 535.2 0 393 244 501 535.2 0 501 245 603 535.2 0 603 246 653 535.2 0 653 247 949 535.2 0 949 248 10803 535.2 0 10803 249 24863 535.2 0 24863 250 20446 535.2 0 20446 251 4892 535.2 0 4892 252 195 535.2 0 195 253 189 535.2 0 189 254 120 535.2 0 120 255 159 535.2 0 159 256 160 535.2 0 160 257 128 535.2 0 128 258 78 535.2 0 78 259 62 535.2 0 62 260 105 535.2 0 105 261 108 535.2 0 108 262 150 535.2 0 150 263 203 535.2 0 203 264 65 535.2 0 65 265 87 535.2 0 87 266 85 535.2 0 85 267 61 535.2 0 61 268 85 535.2 0 85 269 102 535.2 0 102 270 112 535.2 0 112 271 135 535.2 0 135 272 119 535.2 0 119 273 105 535.2 0 105 274 84 535.2 0 84 275 84 535.2 0 84 276 100 535.2 0 100 277 96 535.2 0 96 278 105 535.2 0 105 279 198 535.2 0 198 280 329 535.2 0 329 281 273 535.2 0 273 282 539 535.2 0 539 283 1069 535.2 0 1069 284 1492 535.2 0 1492 285 693 535.2 0 693 286 242 535.2 0 242 287 316 535.2 0 316 288 286 535.2 0 286 289 139 535.2 0 139 290 106 535.2 0 106 291 189 535.2 0 189 292 222 535.2 0 222 293 187 535.2 0 187 294 243 535.2 0 243 295 421 535.2 0 421 296 535 535.2 0 535 297 662 535.2 0 662 298 355 535.2 0 355 299 396 535.2 0 396 300 346 535.2 0 346 301 346 535.2 0 346 Finished in 3.70 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 81,339 Reads with adapters: 22,446 (27.6%) Reads that were too short: 321 (0.4%) Reads written (passing filters): 22,125 (27.2%) Total basepairs processed: 20,245,390 bp Total written (filtered): 5,959,361 bp (29.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22446 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 20 0.0 3 0 0 14 6 16 171 0.0 3 0 131 29 11 17 20619 0.0 3 18658 1585 309 67 18 77 0.0 3 6 57 12 2 19 61 0.0 3 0 0 30 31 20 72 0.0 3 1 0 0 71 21 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 11 0.0 3 0 0 0 11 26 1 0.0 3 0 0 0 1 27 5 0.0 3 0 0 0 5 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 47 16 0.0 3 0 0 0 16 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 65 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 3 0.0 3 0 0 0 3 75 3 0.0 3 0 0 0 3 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 90 3 0.0 3 0 0 0 3 91 70 0.0 3 0 0 2 68 92 12 0.0 3 0 0 0 12 93 5 0.0 3 0 0 0 5 94 13 0.0 3 0 0 1 12 95 6 0.0 3 0 0 0 6 96 163 0.0 3 0 0 3 160 97 2 0.0 3 0 0 0 2 98 191 0.0 3 0 0 6 185 99 110 0.0 3 0 0 4 106 100 9 0.0 3 0 0 1 8 101 18 0.0 3 0 0 1 17 102 6 0.0 3 0 0 0 6 104 5 0.0 3 0 0 0 5 107 2 0.0 3 0 0 1 1 108 3 0.0 3 0 0 1 2 109 4 0.0 3 0 0 0 4 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 114 5 0.0 3 0 0 0 5 115 14 0.0 3 0 0 0 14 116 241 0.0 3 0 0 2 239 117 78 0.0 3 0 0 1 77 118 6 0.0 3 0 0 0 6 119 33 0.0 3 0 0 4 29 120 12 0.0 3 0 0 2 10 121 1 0.0 3 0 0 0 1 122 11 0.0 3 0 0 0 11 123 1 0.0 3 0 0 0 1 124 8 0.0 3 0 0 0 8 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 1 1 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 139 4 0.0 3 0 0 0 4 141 4 0.0 3 0 0 1 3 142 12 0.0 3 0 0 1 11 143 3 0.0 3 0 0 0 3 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 9 0.0 3 0 0 0 9 148 1 0.0 3 0 0 0 1 149 7 0.0 3 0 0 1 6 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 166 4 0.0 3 0 0 0 4 167 17 0.0 3 0 0 0 17 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 9 0.0 3 0 0 0 9 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 3 0.0 3 0 0 0 3 196 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 3 0.0 3 0 0 0 3 203 30 0.0 3 0 0 0 30 204 3 0.0 3 0 0 0 3 205 2 0.0 3 0 0 0 2 206 7 0.0 3 0 0 0 7 207 5 0.0 3 0 0 0 5 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 6 0.0 3 0 0 0 6 217 10 0.0 3 0 0 0 10 218 25 0.0 3 0 0 1 24 219 4 0.0 3 0 0 1 3 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 6 0.0 3 0 0 1 5 229 2 0.0 3 0 0 0 2 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 241 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 22,125 Reads with adapters: 84 (0.4%) Reads written (passing filters): 22,125 (100.0%) Total basepairs processed: 5,959,361 bp Total written (filtered): 5,959,102 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 84 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.9% C: 64.3% G: 15.5% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 345.7 0 79 4 4 86.4 0 4 6 1 5.4 0 1