This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.14 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 207,304 (9.5%) Reads that were too short: 141,345 (6.4%) Reads written (passing filters): 65,959 (3.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,407,474 bp (2.6%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 207304 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1162 535.2 0 1162 7 20590 535.2 0 20590 8 20562 535.2 0 20562 9 33 535.2 0 33 10 19 535.2 0 19 11 22 535.2 0 22 12 17 535.2 0 17 13 29 535.2 0 29 14 24 535.2 0 24 15 23 535.2 0 23 16 21 535.2 0 21 17 29 535.2 0 29 18 25 535.2 0 25 19 22 535.2 0 22 20 20 535.2 0 20 21 28 535.2 0 28 22 20 535.2 0 20 23 23 535.2 0 23 24 23 535.2 0 23 25 26 535.2 0 26 26 17 535.2 0 17 27 23 535.2 0 23 28 25 535.2 0 25 29 31 535.2 0 31 30 14 535.2 0 14 31 18 535.2 0 18 32 15 535.2 0 15 33 28 535.2 0 28 34 13 535.2 0 13 35 23 535.2 0 23 36 19 535.2 0 19 37 24 535.2 0 24 38 13 535.2 0 13 39 17 535.2 0 17 40 26 535.2 0 26 41 25 535.2 0 25 42 35 535.2 0 35 43 19 535.2 0 19 44 40 535.2 0 40 45 19 535.2 0 19 46 23 535.2 0 23 47 18 535.2 0 18 48 28 535.2 0 28 49 19 535.2 0 19 50 27 535.2 0 27 51 43 535.2 0 43 52 36 535.2 0 36 53 35 535.2 0 35 54 82 535.2 0 82 55 65 535.2 0 65 56 70 535.2 0 70 57 44 535.2 0 44 58 49 535.2 0 49 59 43 535.2 0 43 60 57 535.2 0 57 61 112 535.2 0 112 62 155 535.2 0 155 63 64 535.2 0 64 64 68 535.2 0 68 65 104 535.2 0 104 66 48 535.2 0 48 67 44 535.2 0 44 68 93 535.2 0 93 69 121 535.2 0 121 70 101 535.2 0 101 71 81 535.2 0 81 72 130 535.2 0 130 73 151 535.2 0 151 74 220 535.2 0 220 75 3702 535.2 0 3702 76 5802 535.2 0 5802 77 6084 535.2 0 6084 78 64 535.2 0 64 79 33 535.2 0 33 80 27 535.2 0 27 81 26 535.2 0 26 82 30 535.2 0 30 83 22 535.2 0 22 84 21 535.2 0 21 85 25 535.2 0 25 86 18 535.2 0 18 87 30 535.2 0 30 88 30 535.2 0 30 89 18 535.2 0 18 90 23 535.2 0 23 91 47 535.2 0 47 92 17 535.2 0 17 93 10 535.2 0 10 94 30 535.2 0 30 95 62 535.2 0 62 96 22 535.2 0 22 97 11 535.2 0 11 98 41 535.2 0 41 99 33 535.2 0 33 100 19 535.2 0 19 101 28 535.2 0 28 102 27 535.2 0 27 103 28 535.2 0 28 104 35 535.2 0 35 105 35 535.2 0 35 106 26 535.2 0 26 107 25 535.2 0 25 108 20 535.2 0 20 109 18 535.2 0 18 110 18 535.2 0 18 111 16 535.2 0 16 112 13 535.2 0 13 113 20 535.2 0 20 114 20 535.2 0 20 115 31 535.2 0 31 116 16 535.2 0 16 117 22 535.2 0 22 118 29 535.2 0 29 119 34 535.2 0 34 120 26 535.2 0 26 121 27 535.2 0 27 122 24 535.2 0 24 123 23 535.2 0 23 124 25 535.2 0 25 125 24 535.2 0 24 126 40 535.2 0 40 127 93 535.2 0 93 128 33 535.2 0 33 129 31 535.2 0 31 130 22 535.2 0 22 131 25 535.2 0 25 132 30 535.2 0 30 133 25 535.2 0 25 134 32 535.2 0 32 135 32 535.2 0 32 136 37 535.2 0 37 137 64 535.2 0 64 138 33 535.2 0 33 139 38 535.2 0 38 140 28 535.2 0 28 141 18 535.2 0 18 142 55 535.2 0 55 143 33 535.2 0 33 144 40 535.2 0 40 145 14 535.2 0 14 146 33 535.2 0 33 147 33 535.2 0 33 148 22 535.2 0 22 149 18 535.2 0 18 150 35 535.2 0 35 151 24 535.2 0 24 152 12 535.2 0 12 153 16 535.2 0 16 154 33 535.2 0 33 155 28 535.2 0 28 156 63 535.2 0 63 157 28 535.2 0 28 158 40 535.2 0 40 159 29 535.2 0 29 160 23 535.2 0 23 161 39 535.2 0 39 162 50 535.2 0 50 163 63 535.2 0 63 164 52 535.2 0 52 165 54 535.2 0 54 166 50 535.2 0 50 167 44 535.2 0 44 168 55 535.2 0 55 169 41 535.2 0 41 170 31 535.2 0 31 171 25 535.2 0 25 172 37 535.2 0 37 173 35 535.2 0 35 174 20 535.2 0 20 175 28 535.2 0 28 176 35 535.2 0 35 177 42 535.2 0 42 178 28 535.2 0 28 179 43 535.2 0 43 180 54 535.2 0 54 181 51 535.2 0 51 182 76 535.2 0 76 183 43 535.2 0 43 184 44 535.2 0 44 185 42 535.2 0 42 186 45 535.2 0 45 187 73 535.2 0 73 188 80 535.2 0 80 189 55 535.2 0 55 190 57 535.2 0 57 191 50 535.2 0 50 192 49 535.2 0 49 193 96 535.2 0 96 194 122 535.2 0 122 195 108 535.2 0 108 196 82 535.2 0 82 197 209 535.2 0 209 198 209 535.2 0 209 199 185 535.2 0 185 200 57 535.2 0 57 201 90 535.2 0 90 202 56 535.2 0 56 203 91 535.2 0 91 204 83 535.2 0 83 205 80 535.2 0 80 206 45 535.2 0 45 207 47 535.2 0 47 208 46 535.2 0 46 209 29 535.2 0 29 210 53 535.2 0 53 211 123 535.2 0 123 212 58 535.2 0 58 213 220 535.2 0 220 214 95 535.2 0 95 215 66 535.2 0 66 216 55 535.2 0 55 217 132 535.2 0 132 218 347 535.2 0 347 219 215 535.2 0 215 220 136 535.2 0 136 221 166 535.2 0 166 222 95 535.2 0 95 223 331 535.2 0 331 224 287 535.2 0 287 225 383 535.2 0 383 226 135 535.2 0 135 227 142 535.2 0 142 228 174 535.2 0 174 229 193 535.2 0 193 230 135 535.2 0 135 231 170 535.2 0 170 232 200 535.2 0 200 233 319 535.2 0 319 234 434 535.2 0 434 235 732 535.2 0 732 236 862 535.2 0 862 237 1370 535.2 0 1370 238 17629 535.2 0 17629 239 9429 535.2 0 9429 240 18508 535.2 0 18508 241 404 535.2 0 404 242 344 535.2 0 344 243 399 535.2 0 399 244 397 535.2 0 397 245 545 535.2 0 545 246 590 535.2 0 590 247 899 535.2 0 899 248 13174 535.2 0 13174 249 32519 535.2 0 32519 250 26005 535.2 0 26005 251 6060 535.2 0 6060 252 47 535.2 0 47 253 36 535.2 0 36 254 26 535.2 0 26 255 32 535.2 0 32 256 20 535.2 0 20 257 24 535.2 0 24 258 29 535.2 0 29 259 22 535.2 0 22 260 83 535.2 0 83 261 31 535.2 0 31 262 19 535.2 0 19 263 39 535.2 0 39 264 23 535.2 0 23 265 25 535.2 0 25 266 28 535.2 0 28 267 31 535.2 0 31 268 31 535.2 0 31 269 52 535.2 0 52 270 39 535.2 0 39 271 65 535.2 0 65 272 59 535.2 0 59 273 26 535.2 0 26 274 25 535.2 0 25 275 30 535.2 0 30 276 33 535.2 0 33 277 30 535.2 0 30 278 53 535.2 0 53 279 101 535.2 0 101 280 185 535.2 0 185 281 152 535.2 0 152 282 439 535.2 0 439 283 950 535.2 0 950 284 1017 535.2 0 1017 285 296 535.2 0 296 286 78 535.2 0 78 287 82 535.2 0 82 288 56 535.2 0 56 289 45 535.2 0 45 290 54 535.2 0 54 291 112 535.2 0 112 292 56 535.2 0 56 293 71 535.2 0 71 294 190 535.2 0 190 295 500 535.2 0 500 296 255 535.2 0 255 297 254 535.2 0 254 298 84 535.2 0 84 299 121 535.2 0 121 300 110 535.2 0 110 301 172 535.2 0 172 Finished in 3.37 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 65,959 Reads with adapters: 22,921 (34.8%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 22,909 (34.7%) Total basepairs processed: 17,407,474 bp Total written (filtered): 6,243,476 bp (35.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22921 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 10 19 280 0.0 3 0 280 20 21804 0.0 4 21469 323 9 2 1 21 74 0.0 4 23 51 22 601 0.0 4 584 11 5 1 23 17 0.0 4 0 2 0 13 2 25 2 0.0 4 1 0 0 0 1 59 14 0.0 4 0 0 0 0 14 92 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 105 0.0 4 0 0 0 0 105 131 1 0.0 4 0 1 157 1 0.0 4 1 160 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 22,909 Reads with adapters: 2,223 (9.7%) Reads written (passing filters): 22,909 (100.0%) Total basepairs processed: 6,243,476 bp Total written (filtered): 6,213,765 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2223 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 3.6% G: 92.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 358.0 0 19 4 14 89.5 0 14 6 2 5.6 0 2 8 1 0.3 0 1 9 23 0.1 0 0 23 10 221 0.0 1 8 213 11 1902 0.0 1 5 1897 12 4 0.0 1 0 4 15 1 0.0 1 0 1 151 1 0.0 1 0 1 153 1 0.0 1 0 1 154 1 0.0 1 1 173 26 0.0 1 23 3 174 5 0.0 1 2 3 175 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.