This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.00 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 159,116 (7.3%) Reads that were too short: 22,188 (1.0%) Reads written (passing filters): 136,928 (6.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,697,537 bp (5.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 159116 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 913 535.2 0 913 7 5093 535.2 0 5093 8 52159 535.2 0 52159 9 44146 535.2 0 44146 10 394 535.2 0 394 11 48 535.2 0 48 12 56 535.2 0 56 13 41 535.2 0 41 14 64 535.2 0 64 15 42 535.2 0 42 16 41 535.2 0 41 17 38 535.2 0 38 18 42 535.2 0 42 19 58 535.2 0 58 20 56 535.2 0 56 21 41 535.2 0 41 22 37 535.2 0 37 23 53 535.2 0 53 24 51 535.2 0 51 25 28 535.2 0 28 26 27 535.2 0 27 27 48 535.2 0 48 28 41 535.2 0 41 29 73 535.2 0 73 30 38 535.2 0 38 31 44 535.2 0 44 32 28 535.2 0 28 33 38 535.2 0 38 34 42 535.2 0 42 35 49 535.2 0 49 36 73 535.2 0 73 37 66 535.2 0 66 38 49 535.2 0 49 39 66 535.2 0 66 40 74 535.2 0 74 41 66 535.2 0 66 42 44 535.2 0 44 43 45 535.2 0 45 44 54 535.2 0 54 45 42 535.2 0 42 46 60 535.2 0 60 47 30 535.2 0 30 48 51 535.2 0 51 49 61 535.2 0 61 50 53 535.2 0 53 51 63 535.2 0 63 52 54 535.2 0 54 53 109 535.2 0 109 54 73 535.2 0 73 55 93 535.2 0 93 56 63 535.2 0 63 57 47 535.2 0 47 58 69 535.2 0 69 59 94 535.2 0 94 60 71 535.2 0 71 61 72 535.2 0 72 62 59 535.2 0 59 63 172 535.2 0 172 64 167 535.2 0 167 65 126 535.2 0 126 66 47 535.2 0 47 67 106 535.2 0 106 68 135 535.2 0 135 69 118 535.2 0 118 70 77 535.2 0 77 71 107 535.2 0 107 72 316 535.2 0 316 73 1415 535.2 0 1415 74 1370 535.2 0 1370 75 1049 535.2 0 1049 76 54 535.2 0 54 77 48 535.2 0 48 78 54 535.2 0 54 79 59 535.2 0 59 80 42 535.2 0 42 81 50 535.2 0 50 82 45 535.2 0 45 83 39 535.2 0 39 84 61 535.2 0 61 85 104 535.2 0 104 86 113 535.2 0 113 87 143 535.2 0 143 88 297 535.2 0 297 89 114 535.2 0 114 90 102 535.2 0 102 91 90 535.2 0 90 92 73 535.2 0 73 93 59 535.2 0 59 94 63 535.2 0 63 95 72 535.2 0 72 96 70 535.2 0 70 97 48 535.2 0 48 98 41 535.2 0 41 99 59 535.2 0 59 100 82 535.2 0 82 101 68 535.2 0 68 102 66 535.2 0 66 103 45 535.2 0 45 104 58 535.2 0 58 105 118 535.2 0 118 106 72 535.2 0 72 107 72 535.2 0 72 108 88 535.2 0 88 109 117 535.2 0 117 110 111 535.2 0 111 111 74 535.2 0 74 112 38 535.2 0 38 113 48 535.2 0 48 114 62 535.2 0 62 115 77 535.2 0 77 116 74 535.2 0 74 117 45 535.2 0 45 118 60 535.2 0 60 119 49 535.2 0 49 120 42 535.2 0 42 121 65 535.2 0 65 122 74 535.2 0 74 123 57 535.2 0 57 124 50 535.2 0 50 125 43 535.2 0 43 126 65 535.2 0 65 127 87 535.2 0 87 128 98 535.2 0 98 129 114 535.2 0 114 130 153 535.2 0 153 131 95 535.2 0 95 132 86 535.2 0 86 133 87 535.2 0 87 134 60 535.2 0 60 135 127 535.2 0 127 136 166 535.2 0 166 137 135 535.2 0 135 138 78 535.2 0 78 139 57 535.2 0 57 140 82 535.2 0 82 141 104 535.2 0 104 142 86 535.2 0 86 143 101 535.2 0 101 144 191 535.2 0 191 145 158 535.2 0 158 146 251 535.2 0 251 147 334 535.2 0 334 148 424 535.2 0 424 149 132 535.2 0 132 150 177 535.2 0 177 151 90 535.2 0 90 152 112 535.2 0 112 153 147 535.2 0 147 154 115 535.2 0 115 155 225 535.2 0 225 156 289 535.2 0 289 157 387 535.2 0 387 158 950 535.2 0 950 159 738 535.2 0 738 160 589 535.2 0 589 161 352 535.2 0 352 162 268 535.2 0 268 163 81 535.2 0 81 164 76 535.2 0 76 165 133 535.2 0 133 166 232 535.2 0 232 167 322 535.2 0 322 168 166 535.2 0 166 169 218 535.2 0 218 170 217 535.2 0 217 171 275 535.2 0 275 172 198 535.2 0 198 173 229 535.2 0 229 174 2756 535.2 0 2756 175 2017 535.2 0 2017 176 2147 535.2 0 2147 177 197 535.2 0 197 178 96 535.2 0 96 179 84 535.2 0 84 180 313 535.2 0 313 181 165 535.2 0 165 182 208 535.2 0 208 183 107 535.2 0 107 184 116 535.2 0 116 185 211 535.2 0 211 186 437 535.2 0 437 187 1529 535.2 0 1529 188 717 535.2 0 717 189 511 535.2 0 511 190 83 535.2 0 83 191 80 535.2 0 80 192 102 535.2 0 102 193 86 535.2 0 86 194 142 535.2 0 142 195 90 535.2 0 90 196 72 535.2 0 72 197 64 535.2 0 64 198 75 535.2 0 75 199 68 535.2 0 68 200 110 535.2 0 110 201 98 535.2 0 98 202 94 535.2 0 94 203 133 535.2 0 133 204 241 535.2 0 241 205 183 535.2 0 183 206 186 535.2 0 186 207 95 535.2 0 95 208 68 535.2 0 68 209 139 535.2 0 139 210 78 535.2 0 78 211 73 535.2 0 73 212 61 535.2 0 61 213 79 535.2 0 79 214 138 535.2 0 138 215 164 535.2 0 164 216 140 535.2 0 140 217 123 535.2 0 123 218 116 535.2 0 116 219 104 535.2 0 104 220 125 535.2 0 125 221 99 535.2 0 99 222 114 535.2 0 114 223 124 535.2 0 124 224 112 535.2 0 112 225 78 535.2 0 78 226 98 535.2 0 98 227 280 535.2 0 280 228 179 535.2 0 179 229 162 535.2 0 162 230 92 535.2 0 92 231 131 535.2 0 131 232 173 535.2 0 173 233 169 535.2 0 169 234 135 535.2 0 135 235 123 535.2 0 123 236 179 535.2 0 179 237 210 535.2 0 210 238 229 535.2 0 229 239 258 535.2 0 258 240 113 535.2 0 113 241 104 535.2 0 104 242 101 535.2 0 101 243 137 535.2 0 137 244 152 535.2 0 152 245 135 535.2 0 135 246 179 535.2 0 179 247 220 535.2 0 220 248 458 535.2 0 458 249 386 535.2 0 386 250 269 535.2 0 269 251 1232 535.2 0 1232 252 973 535.2 0 973 253 1518 535.2 0 1518 254 183 535.2 0 183 255 202 535.2 0 202 256 100 535.2 0 100 257 125 535.2 0 125 258 108 535.2 0 108 259 146 535.2 0 146 260 379 535.2 0 379 261 523 535.2 0 523 262 683 535.2 0 683 263 370 535.2 0 370 264 179 535.2 0 179 265 85 535.2 0 85 266 77 535.2 0 77 267 111 535.2 0 111 268 162 535.2 0 162 269 201 535.2 0 201 270 186 535.2 0 186 271 144 535.2 0 144 272 269 535.2 0 269 273 188 535.2 0 188 274 169 535.2 0 169 275 131 535.2 0 131 276 115 535.2 0 115 277 164 535.2 0 164 278 203 535.2 0 203 279 273 535.2 0 273 280 253 535.2 0 253 281 281 535.2 0 281 282 304 535.2 0 304 283 234 535.2 0 234 284 120 535.2 0 120 285 155 535.2 0 155 286 230 535.2 0 230 287 153 535.2 0 153 288 174 535.2 0 174 289 125 535.2 0 125 290 188 535.2 0 188 291 354 535.2 0 354 292 324 535.2 0 324 293 216 535.2 0 216 294 208 535.2 0 208 295 237 535.2 0 237 296 264 535.2 0 264 297 270 535.2 0 270 298 329 535.2 0 329 299 354 535.2 0 354 300 303 535.2 0 303 301 374 535.2 0 374 Finished in 5.98 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 136,928 Reads with adapters: 54,028 (39.5%) Reads that were too short: 161 (0.1%) Reads written (passing filters): 53,867 (39.3%) Total basepairs processed: 35,697,537 bp Total written (filtered): 14,851,858 bp (41.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 54028 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 55 0.0 3 0 0 33 22 16 579 0.0 3 0 447 79 53 17 52494 0.0 3 48288 3357 664 185 18 161 0.0 3 18 108 19 16 19 196 0.0 3 10 2 101 83 20 243 0.0 3 1 0 1 241 21 4 0.0 3 0 0 2 2 23 2 0.0 3 0 2 24 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 7 0.0 3 0 0 0 7 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 10 0.0 3 0 0 1 9 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 46 3 0.0 3 0 0 0 3 47 7 0.0 3 0 0 0 7 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 8 0.0 3 0 0 1 7 55 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 58 3 0.0 3 0 0 0 3 59 3 0.0 3 0 0 1 2 60 3 0.0 3 0 0 1 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 1 0 0 2 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 1 2 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 3 0.0 3 0 0 0 3 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 3 0.0 3 0 0 1 2 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 1 1 106 6 0.0 3 0 0 1 5 107 1 0.0 3 0 0 1 108 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 3 0.0 3 0 0 0 3 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 1 1 133 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 156 4 0.0 3 0 0 0 4 157 4 0.0 3 0 0 0 4 161 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 163 6 0.0 3 0 0 0 6 164 3 0.0 3 0 0 1 2 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 3 0.0 3 0 0 0 3 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 7 0.0 3 0 0 0 7 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 1 1 255 1 0.0 3 0 0 1 259 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 278 4 0.0 3 0 0 0 4 279 6 0.0 3 0 0 0 6 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 282 8 0.0 3 0 0 0 8 283 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.85 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 53,867 Reads with adapters: 256 (0.5%) Reads written (passing filters): 53,867 (100.0%) Total basepairs processed: 14,851,858 bp Total written (filtered): 14,850,997 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 256 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.0% C: 11.7% G: 25.8% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 197 841.7 0 197 4 50 210.4 0 50 5 5 52.6 0 5 11 3 0.0 1 0 3 12 1 0.0 1 0 1