This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.93 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 136,990 (6.2%) Reads that were too short: 9,548 (0.4%) Reads written (passing filters): 127,442 (5.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,370,079 bp (5.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 136990 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 887 535.2 0 887 7 3681 535.2 0 3681 8 48394 535.2 0 48394 9 56481 535.2 0 56481 10 105 535.2 0 105 11 29 535.2 0 29 12 43 535.2 0 43 13 32 535.2 0 32 14 55 535.2 0 55 15 38 535.2 0 38 16 34 535.2 0 34 17 34 535.2 0 34 18 35 535.2 0 35 19 44 535.2 0 44 20 43 535.2 0 43 21 46 535.2 0 46 22 29 535.2 0 29 23 41 535.2 0 41 24 34 535.2 0 34 25 34 535.2 0 34 26 29 535.2 0 29 27 39 535.2 0 39 28 42 535.2 0 42 29 30 535.2 0 30 30 35 535.2 0 35 31 42 535.2 0 42 32 42 535.2 0 42 33 29 535.2 0 29 34 31 535.2 0 31 35 28 535.2 0 28 36 34 535.2 0 34 37 40 535.2 0 40 38 40 535.2 0 40 39 43 535.2 0 43 40 39 535.2 0 39 41 40 535.2 0 40 42 41 535.2 0 41 43 32 535.2 0 32 44 43 535.2 0 43 45 29 535.2 0 29 46 36 535.2 0 36 47 37 535.2 0 37 48 35 535.2 0 35 49 28 535.2 0 28 50 35 535.2 0 35 51 26 535.2 0 26 52 43 535.2 0 43 53 37 535.2 0 37 54 371 535.2 0 371 55 535 535.2 0 535 56 55 535.2 0 55 57 33 535.2 0 33 58 46 535.2 0 46 59 44 535.2 0 44 60 35 535.2 0 35 61 49 535.2 0 49 62 41 535.2 0 41 63 26 535.2 0 26 64 47 535.2 0 47 65 37 535.2 0 37 66 40 535.2 0 40 67 54 535.2 0 54 68 62 535.2 0 62 69 67 535.2 0 67 70 47 535.2 0 47 71 83 535.2 0 83 72 420 535.2 0 420 73 1398 535.2 0 1398 74 1378 535.2 0 1378 75 1029 535.2 0 1029 76 42 535.2 0 42 77 43 535.2 0 43 78 37 535.2 0 37 79 56 535.2 0 56 80 34 535.2 0 34 81 18 535.2 0 18 82 29 535.2 0 29 83 27 535.2 0 27 84 37 535.2 0 37 85 49 535.2 0 49 86 66 535.2 0 66 87 59 535.2 0 59 88 75 535.2 0 75 89 62 535.2 0 62 90 60 535.2 0 60 91 46 535.2 0 46 92 44 535.2 0 44 93 45 535.2 0 45 94 43 535.2 0 43 95 43 535.2 0 43 96 33 535.2 0 33 97 32 535.2 0 32 98 35 535.2 0 35 99 47 535.2 0 47 100 52 535.2 0 52 101 40 535.2 0 40 102 28 535.2 0 28 103 29 535.2 0 29 104 34 535.2 0 34 105 79 535.2 0 79 106 65 535.2 0 65 107 29 535.2 0 29 108 57 535.2 0 57 109 64 535.2 0 64 110 47 535.2 0 47 111 35 535.2 0 35 112 35 535.2 0 35 113 33 535.2 0 33 114 27 535.2 0 27 115 46 535.2 0 46 116 51 535.2 0 51 117 36 535.2 0 36 118 40 535.2 0 40 119 48 535.2 0 48 120 32 535.2 0 32 121 43 535.2 0 43 122 39 535.2 0 39 123 32 535.2 0 32 124 25 535.2 0 25 125 38 535.2 0 38 126 32 535.2 0 32 127 44 535.2 0 44 128 49 535.2 0 49 129 62 535.2 0 62 130 45 535.2 0 45 131 38 535.2 0 38 132 28 535.2 0 28 133 57 535.2 0 57 134 44 535.2 0 44 135 48 535.2 0 48 136 127 535.2 0 127 137 46 535.2 0 46 138 57 535.2 0 57 139 30 535.2 0 30 140 41 535.2 0 41 141 43 535.2 0 43 142 45 535.2 0 45 143 38 535.2 0 38 144 64 535.2 0 64 145 79 535.2 0 79 146 93 535.2 0 93 147 127 535.2 0 127 148 252 535.2 0 252 149 68 535.2 0 68 150 176 535.2 0 176 151 41 535.2 0 41 152 46 535.2 0 46 153 46 535.2 0 46 154 44 535.2 0 44 155 79 535.2 0 79 156 70 535.2 0 70 157 87 535.2 0 87 158 312 535.2 0 312 159 217 535.2 0 217 160 166 535.2 0 166 161 161 535.2 0 161 162 55 535.2 0 55 163 33 535.2 0 33 164 45 535.2 0 45 165 45 535.2 0 45 166 64 535.2 0 64 167 49 535.2 0 49 168 51 535.2 0 51 169 117 535.2 0 117 170 99 535.2 0 99 171 172 535.2 0 172 172 87 535.2 0 87 173 97 535.2 0 97 174 965 535.2 0 965 175 574 535.2 0 574 176 678 535.2 0 678 177 56 535.2 0 56 178 49 535.2 0 49 179 38 535.2 0 38 180 218 535.2 0 218 181 67 535.2 0 67 182 61 535.2 0 61 183 39 535.2 0 39 184 63 535.2 0 63 185 56 535.2 0 56 186 144 535.2 0 144 187 411 535.2 0 411 188 201 535.2 0 201 189 177 535.2 0 177 190 53 535.2 0 53 191 59 535.2 0 59 192 35 535.2 0 35 193 51 535.2 0 51 194 70 535.2 0 70 195 34 535.2 0 34 196 45 535.2 0 45 197 42 535.2 0 42 198 34 535.2 0 34 199 48 535.2 0 48 200 50 535.2 0 50 201 41 535.2 0 41 202 49 535.2 0 49 203 50 535.2 0 50 204 86 535.2 0 86 205 74 535.2 0 74 206 62 535.2 0 62 207 50 535.2 0 50 208 30 535.2 0 30 209 36 535.2 0 36 210 29 535.2 0 29 211 39 535.2 0 39 212 28 535.2 0 28 213 34 535.2 0 34 214 48 535.2 0 48 215 53 535.2 0 53 216 47 535.2 0 47 217 43 535.2 0 43 218 43 535.2 0 43 219 50 535.2 0 50 220 33 535.2 0 33 221 44 535.2 0 44 222 45 535.2 0 45 223 45 535.2 0 45 224 41 535.2 0 41 225 32 535.2 0 32 226 41 535.2 0 41 227 146 535.2 0 146 228 54 535.2 0 54 229 84 535.2 0 84 230 75 535.2 0 75 231 60 535.2 0 60 232 70 535.2 0 70 233 68 535.2 0 68 234 49 535.2 0 49 235 49 535.2 0 49 236 57 535.2 0 57 237 61 535.2 0 61 238 92 535.2 0 92 239 84 535.2 0 84 240 43 535.2 0 43 241 53 535.2 0 53 242 41 535.2 0 41 243 59 535.2 0 59 244 59 535.2 0 59 245 47 535.2 0 47 246 67 535.2 0 67 247 73 535.2 0 73 248 134 535.2 0 134 249 90 535.2 0 90 250 114 535.2 0 114 251 915 535.2 0 915 252 768 535.2 0 768 253 982 535.2 0 982 254 45 535.2 0 45 255 73 535.2 0 73 256 44 535.2 0 44 257 37 535.2 0 37 258 58 535.2 0 58 259 69 535.2 0 69 260 103 535.2 0 103 261 119 535.2 0 119 262 140 535.2 0 140 263 105 535.2 0 105 264 62 535.2 0 62 265 40 535.2 0 40 266 38 535.2 0 38 267 42 535.2 0 42 268 59 535.2 0 59 269 62 535.2 0 62 270 64 535.2 0 64 271 56 535.2 0 56 272 69 535.2 0 69 273 64 535.2 0 64 274 87 535.2 0 87 275 90 535.2 0 90 276 67 535.2 0 67 277 79 535.2 0 79 278 114 535.2 0 114 279 139 535.2 0 139 280 106 535.2 0 106 281 84 535.2 0 84 282 100 535.2 0 100 283 85 535.2 0 85 284 51 535.2 0 51 285 106 535.2 0 106 286 79 535.2 0 79 287 67 535.2 0 67 288 74 535.2 0 74 289 60 535.2 0 60 290 69 535.2 0 69 291 81 535.2 0 81 292 89 535.2 0 89 293 56 535.2 0 56 294 88 535.2 0 88 295 170 535.2 0 170 296 86 535.2 0 86 297 115 535.2 0 115 298 108 535.2 0 108 299 111 535.2 0 111 300 146 535.2 0 146 301 166 535.2 0 166 Finished in 6.16 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 127,442 Reads with adapters: 60,447 (47.4%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 60,425 (47.4%) Total basepairs processed: 35,370,079 bp Total written (filtered): 16,374,915 bp (46.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60447 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 30 0.0 3 0 0 28 1 1 19 712 0.0 3 0 696 16 20 58779 0.0 4 57878 858 34 5 4 21 156 0.0 4 30 119 7 22 14 0.0 4 1 4 8 0 1 23 111 0.0 4 59 2 0 45 5 24 2 0.0 4 0 1 0 1 26 1 0.0 4 1 30 1 0.0 4 0 1 33 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 14 0.0 4 0 0 0 0 14 127 599 0.0 4 0 0 0 0 599 155 11 0.0 4 0 0 0 0 11 157 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.13 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 60,425 Reads with adapters: 8,814 (14.6%) Reads written (passing filters): 60,425 (100.0%) Total basepairs processed: 16,374,915 bp Total written (filtered): 16,284,427 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8814 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.1% G: 94.6% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 21 944.1 0 21 4 12 236.0 0 12 5 3 59.0 0 3 6 3 14.8 0 3 7 4 3.7 0 4 8 2 0.9 0 2 9 3888 0.2 0 125 3763 10 3471 0.1 1 138 3333 11 1352 0.0 1 13 1339 12 6 0.0 1 0 6 13 7 0.0 1 0 7 18 2 0.0 1 1 1 22 12 0.0 1 11 1 147 2 0.0 1 2 150 13 0.0 1 0 13 151 1 0.0 1 0 1 152 1 0.0 1 0 1 153 1 0.0 1 0 1 158 1 0.0 1 0 1 169 1 0.0 1 1 175 1 0.0 1 1 189 1 0.0 1 0 1 208 9 0.0 1 2 7 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.