This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.95 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 90,607 (4.1%) Reads that were too short: 18,753 (0.9%) Reads written (passing filters): 71,854 (3.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,868,493 bp (2.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 90607 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1081 535.2 0 1081 7 21984 535.2 0 21984 8 18571 535.2 0 18571 9 39 535.2 0 39 10 70 535.2 0 70 11 336 535.2 0 336 12 135 535.2 0 135 13 314 535.2 0 314 14 228 535.2 0 228 15 574 535.2 0 574 16 48 535.2 0 48 17 18 535.2 0 18 18 23 535.2 0 23 19 28 535.2 0 28 20 13 535.2 0 13 21 22 535.2 0 22 22 26 535.2 0 26 23 37 535.2 0 37 24 53 535.2 0 53 25 130 535.2 0 130 26 174 535.2 0 174 27 101 535.2 0 101 28 23 535.2 0 23 29 23 535.2 0 23 30 29 535.2 0 29 31 39 535.2 0 39 32 17 535.2 0 17 33 33 535.2 0 33 34 27 535.2 0 27 35 33 535.2 0 33 36 18 535.2 0 18 37 21 535.2 0 21 38 23 535.2 0 23 39 23 535.2 0 23 40 21 535.2 0 21 41 33 535.2 0 33 42 34 535.2 0 34 43 29 535.2 0 29 44 52 535.2 0 52 45 21 535.2 0 21 46 17 535.2 0 17 47 28 535.2 0 28 48 32 535.2 0 32 49 26 535.2 0 26 50 37 535.2 0 37 51 19 535.2 0 19 52 15 535.2 0 15 53 35 535.2 0 35 54 39 535.2 0 39 55 26 535.2 0 26 56 46 535.2 0 46 57 55 535.2 0 55 58 35 535.2 0 35 59 27 535.2 0 27 60 80 535.2 0 80 61 107 535.2 0 107 62 103 535.2 0 103 63 24 535.2 0 24 64 24 535.2 0 24 65 26 535.2 0 26 66 34 535.2 0 34 67 31 535.2 0 31 68 26 535.2 0 26 69 27 535.2 0 27 70 23 535.2 0 23 71 37 535.2 0 37 72 37 535.2 0 37 73 26 535.2 0 26 74 19 535.2 0 19 75 32 535.2 0 32 76 49 535.2 0 49 77 40 535.2 0 40 78 54 535.2 0 54 79 70 535.2 0 70 80 54 535.2 0 54 81 54 535.2 0 54 82 140 535.2 0 140 83 151 535.2 0 151 84 459 535.2 0 459 85 23 535.2 0 23 86 32 535.2 0 32 87 28 535.2 0 28 88 34 535.2 0 34 89 58 535.2 0 58 90 28 535.2 0 28 91 26 535.2 0 26 92 23 535.2 0 23 93 29 535.2 0 29 94 22 535.2 0 22 95 46 535.2 0 46 96 40 535.2 0 40 97 44 535.2 0 44 98 45 535.2 0 45 99 36 535.2 0 36 100 25 535.2 0 25 101 38 535.2 0 38 102 79 535.2 0 79 103 63 535.2 0 63 104 86 535.2 0 86 105 61 535.2 0 61 106 46 535.2 0 46 107 112 535.2 0 112 108 89 535.2 0 89 109 36 535.2 0 36 110 48 535.2 0 48 111 86 535.2 0 86 112 108 535.2 0 108 113 63 535.2 0 63 114 44 535.2 0 44 115 48 535.2 0 48 116 46 535.2 0 46 117 36 535.2 0 36 118 48 535.2 0 48 119 68 535.2 0 68 120 80 535.2 0 80 121 56 535.2 0 56 122 115 535.2 0 115 123 166 535.2 0 166 124 107 535.2 0 107 125 93 535.2 0 93 126 45 535.2 0 45 127 52 535.2 0 52 128 60 535.2 0 60 129 46 535.2 0 46 130 38 535.2 0 38 131 56 535.2 0 56 132 115 535.2 0 115 133 141 535.2 0 141 134 229 535.2 0 229 135 69 535.2 0 69 136 54 535.2 0 54 137 114 535.2 0 114 138 146 535.2 0 146 139 170 535.2 0 170 140 168 535.2 0 168 141 125 535.2 0 125 142 141 535.2 0 141 143 52 535.2 0 52 144 55 535.2 0 55 145 98 535.2 0 98 146 81 535.2 0 81 147 211 535.2 0 211 148 82 535.2 0 82 149 71 535.2 0 71 150 79 535.2 0 79 151 55 535.2 0 55 152 52 535.2 0 52 153 69 535.2 0 69 154 77 535.2 0 77 155 111 535.2 0 111 156 172 535.2 0 172 157 223 535.2 0 223 158 136 535.2 0 136 159 182 535.2 0 182 160 1101 535.2 0 1101 161 1334 535.2 0 1334 162 1670 535.2 0 1670 163 306 535.2 0 306 164 119 535.2 0 119 165 59 535.2 0 59 166 56 535.2 0 56 167 88 535.2 0 88 168 121 535.2 0 121 169 121 535.2 0 121 170 134 535.2 0 134 171 45 535.2 0 45 172 62 535.2 0 62 173 76 535.2 0 76 174 67 535.2 0 67 175 137 535.2 0 137 176 83 535.2 0 83 177 102 535.2 0 102 178 102 535.2 0 102 179 138 535.2 0 138 180 378 535.2 0 378 181 799 535.2 0 799 182 939 535.2 0 939 183 411 535.2 0 411 184 221 535.2 0 221 185 204 535.2 0 204 186 203 535.2 0 203 187 1816 535.2 0 1816 188 2318 535.2 0 2318 189 5361 535.2 0 5361 190 73 535.2 0 73 191 43 535.2 0 43 192 43 535.2 0 43 193 57 535.2 0 57 194 57 535.2 0 57 195 56 535.2 0 56 196 55 535.2 0 55 197 52 535.2 0 52 198 55 535.2 0 55 199 71 535.2 0 71 200 60 535.2 0 60 201 57 535.2 0 57 202 142 535.2 0 142 203 247 535.2 0 247 204 269 535.2 0 269 205 209 535.2 0 209 206 433 535.2 0 433 207 334 535.2 0 334 208 140 535.2 0 140 209 76 535.2 0 76 210 410 535.2 0 410 211 683 535.2 0 683 212 1087 535.2 0 1087 213 223 535.2 0 223 214 118 535.2 0 118 215 67 535.2 0 67 216 43 535.2 0 43 217 65 535.2 0 65 218 61 535.2 0 61 219 138 535.2 0 138 220 307 535.2 0 307 221 195 535.2 0 195 222 118 535.2 0 118 223 84 535.2 0 84 224 71 535.2 0 71 225 112 535.2 0 112 226 118 535.2 0 118 227 123 535.2 0 123 228 116 535.2 0 116 229 172 535.2 0 172 230 893 535.2 0 893 231 1415 535.2 0 1415 232 1075 535.2 0 1075 233 105 535.2 0 105 234 57 535.2 0 57 235 40 535.2 0 40 236 46 535.2 0 46 237 33 535.2 0 33 238 46 535.2 0 46 239 71 535.2 0 71 240 97 535.2 0 97 241 88 535.2 0 88 242 51 535.2 0 51 243 51 535.2 0 51 244 58 535.2 0 58 245 53 535.2 0 53 246 38 535.2 0 38 247 26 535.2 0 26 248 36 535.2 0 36 249 61 535.2 0 61 250 50 535.2 0 50 251 55 535.2 0 55 252 58 535.2 0 58 253 135 535.2 0 135 254 207 535.2 0 207 255 258 535.2 0 258 256 104 535.2 0 104 257 98 535.2 0 98 258 84 535.2 0 84 259 59 535.2 0 59 260 94 535.2 0 94 261 80 535.2 0 80 262 72 535.2 0 72 263 126 535.2 0 126 264 97 535.2 0 97 265 80 535.2 0 80 266 52 535.2 0 52 267 80 535.2 0 80 268 119 535.2 0 119 269 120 535.2 0 120 270 70 535.2 0 70 271 64 535.2 0 64 272 53 535.2 0 53 273 131 535.2 0 131 274 159 535.2 0 159 275 294 535.2 0 294 276 364 535.2 0 364 277 396 535.2 0 396 278 264 535.2 0 264 279 476 535.2 0 476 280 413 535.2 0 413 281 164 535.2 0 164 282 119 535.2 0 119 283 165 535.2 0 165 284 195 535.2 0 195 285 155 535.2 0 155 286 93 535.2 0 93 287 112 535.2 0 112 288 230 535.2 0 230 289 504 535.2 0 504 290 226 535.2 0 226 291 250 535.2 0 250 292 62 535.2 0 62 293 95 535.2 0 95 294 105 535.2 0 105 295 142 535.2 0 142 296 94 535.2 0 94 297 104 535.2 0 104 298 70 535.2 0 70 299 79 535.2 0 79 300 305 535.2 0 305 301 271 535.2 0 271 Finished in 3.09 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 71,854 Reads with adapters: 22,167 (30.9%) Reads that were too short: 187 (0.3%) Reads written (passing filters): 21,980 (30.6%) Total basepairs processed: 16,868,493 bp Total written (filtered): 6,072,832 bp (36.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22167 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 21 0.0 3 0 0 10 11 16 276 0.0 3 0 219 30 27 17 21285 0.0 3 19362 1561 286 76 18 64 0.0 3 7 46 2 9 19 140 0.0 3 38 20 52 30 20 92 0.0 3 4 2 0 86 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 7 0.0 3 0 0 0 7 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 1 2 52 1 0.0 3 0 0 0 1 54 6 0.0 3 0 0 0 6 57 4 0.0 3 0 0 0 4 59 2 0.0 3 0 0 0 2 62 8 0.0 3 0 0 0 8 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 69 10 0.0 3 0 0 0 10 70 3 0.0 3 0 0 0 3 71 3 0.0 3 0 0 0 3 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 100 3 0.0 3 0 0 0 3 101 3 0.0 3 0 0 1 2 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 1 1 107 1 0.0 3 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 1 112 4 0.0 3 0 0 0 4 113 9 0.0 3 0 0 0 9 114 3 0.0 3 0 0 1 2 115 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 12 0.0 3 0 0 0 12 124 2 0.0 3 0 1 0 1 125 3 0.0 3 0 0 0 3 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 5 0.0 3 0 0 0 5 132 2 0.0 3 0 0 1 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 31 0.0 3 0 0 0 31 142 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 155 3 0.0 3 0 0 0 3 156 3 0.0 3 0 0 0 3 157 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 160 4 0.0 3 0 0 0 4 161 2 0.0 3 0 0 1 1 163 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 5 0.0 3 0 0 0 5 203 1 0.0 3 0 0 0 1 206 4 0.0 3 0 0 0 4 207 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 13 0.0 3 0 0 0 13 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 7 0.0 3 0 0 0 7 282 3 0.0 3 0 0 1 2 289 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.77 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 21,980 Reads with adapters: 90 (0.4%) Reads written (passing filters): 21,980 (100.0%) Total basepairs processed: 6,072,832 bp Total written (filtered): 6,072,557 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 90 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.8% C: 38.9% G: 23.3% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 87 343.4 0 87 4 2 85.9 0 2 6 1 5.4 0 1