This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.74 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 76,557 (3.5%) Reads that were too short: 8,819 (0.4%) Reads written (passing filters): 67,738 (3.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,368,444 bp (2.5%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 76557 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 765 535.2 0 765 7 20181 535.2 0 20181 8 23370 535.2 0 23370 9 30 535.2 0 30 10 25 535.2 0 25 11 61 535.2 0 61 12 32 535.2 0 32 13 74 535.2 0 74 14 75 535.2 0 75 15 90 535.2 0 90 16 32 535.2 0 32 17 19 535.2 0 19 18 11 535.2 0 11 19 20 535.2 0 20 20 11 535.2 0 11 21 23 535.2 0 23 22 8 535.2 0 8 23 20 535.2 0 20 24 11 535.2 0 11 25 136 535.2 0 136 26 204 535.2 0 204 27 15 535.2 0 15 28 22 535.2 0 22 29 11 535.2 0 11 30 13 535.2 0 13 31 18 535.2 0 18 32 22 535.2 0 22 33 20 535.2 0 20 34 13 535.2 0 13 35 9 535.2 0 9 36 10 535.2 0 10 37 12 535.2 0 12 38 8 535.2 0 8 39 9 535.2 0 9 40 10 535.2 0 10 41 20 535.2 0 20 42 25 535.2 0 25 43 17 535.2 0 17 44 26 535.2 0 26 45 15 535.2 0 15 46 10 535.2 0 10 47 28 535.2 0 28 48 18 535.2 0 18 49 18 535.2 0 18 50 19 535.2 0 19 51 17 535.2 0 17 52 12 535.2 0 12 53 24 535.2 0 24 54 19 535.2 0 19 55 16 535.2 0 16 56 23 535.2 0 23 57 26 535.2 0 26 58 21 535.2 0 21 59 18 535.2 0 18 60 53 535.2 0 53 61 40 535.2 0 40 62 41 535.2 0 41 63 18 535.2 0 18 64 19 535.2 0 19 65 25 535.2 0 25 66 18 535.2 0 18 67 12 535.2 0 12 68 15 535.2 0 15 69 21 535.2 0 21 70 21 535.2 0 21 71 19 535.2 0 19 72 15 535.2 0 15 73 18 535.2 0 18 74 25 535.2 0 25 75 25 535.2 0 25 76 19 535.2 0 19 77 32 535.2 0 32 78 20 535.2 0 20 79 33 535.2 0 33 80 43 535.2 0 43 81 52 535.2 0 52 82 229 535.2 0 229 83 203 535.2 0 203 84 877 535.2 0 877 85 20 535.2 0 20 86 40 535.2 0 40 87 47 535.2 0 47 88 33 535.2 0 33 89 50 535.2 0 50 90 36 535.2 0 36 91 19 535.2 0 19 92 14 535.2 0 14 93 22 535.2 0 22 94 24 535.2 0 24 95 43 535.2 0 43 96 33 535.2 0 33 97 24 535.2 0 24 98 40 535.2 0 40 99 23 535.2 0 23 100 37 535.2 0 37 101 39 535.2 0 39 102 42 535.2 0 42 103 36 535.2 0 36 104 66 535.2 0 66 105 37 535.2 0 37 106 38 535.2 0 38 107 77 535.2 0 77 108 76 535.2 0 76 109 41 535.2 0 41 110 36 535.2 0 36 111 65 535.2 0 65 112 97 535.2 0 97 113 48 535.2 0 48 114 35 535.2 0 35 115 28 535.2 0 28 116 33 535.2 0 33 117 35 535.2 0 35 118 39 535.2 0 39 119 48 535.2 0 48 120 73 535.2 0 73 121 40 535.2 0 40 122 64 535.2 0 64 123 83 535.2 0 83 124 67 535.2 0 67 125 105 535.2 0 105 126 31 535.2 0 31 127 37 535.2 0 37 128 45 535.2 0 45 129 48 535.2 0 48 130 38 535.2 0 38 131 42 535.2 0 42 132 117 535.2 0 117 133 114 535.2 0 114 134 184 535.2 0 184 135 38 535.2 0 38 136 37 535.2 0 37 137 60 535.2 0 60 138 64 535.2 0 64 139 50 535.2 0 50 140 80 535.2 0 80 141 50 535.2 0 50 142 59 535.2 0 59 143 39 535.2 0 39 144 50 535.2 0 50 145 100 535.2 0 100 146 39 535.2 0 39 147 209 535.2 0 209 148 40 535.2 0 40 149 35 535.2 0 35 150 32 535.2 0 32 151 31 535.2 0 31 152 37 535.2 0 37 153 32 535.2 0 32 154 35 535.2 0 35 155 44 535.2 0 44 156 52 535.2 0 52 157 62 535.2 0 62 158 50 535.2 0 50 159 64 535.2 0 64 160 277 535.2 0 277 161 219 535.2 0 219 162 324 535.2 0 324 163 68 535.2 0 68 164 40 535.2 0 40 165 46 535.2 0 46 166 27 535.2 0 27 167 50 535.2 0 50 168 99 535.2 0 99 169 114 535.2 0 114 170 150 535.2 0 150 171 30 535.2 0 30 172 47 535.2 0 47 173 62 535.2 0 62 174 53 535.2 0 53 175 66 535.2 0 66 176 49 535.2 0 49 177 60 535.2 0 60 178 38 535.2 0 38 179 64 535.2 0 64 180 115 535.2 0 115 181 169 535.2 0 169 182 229 535.2 0 229 183 163 535.2 0 163 184 137 535.2 0 137 185 185 535.2 0 185 186 188 535.2 0 188 187 2427 535.2 0 2427 188 2762 535.2 0 2762 189 7411 535.2 0 7411 190 58 535.2 0 58 191 41 535.2 0 41 192 43 535.2 0 43 193 40 535.2 0 40 194 40 535.2 0 40 195 26 535.2 0 26 196 39 535.2 0 39 197 43 535.2 0 43 198 51 535.2 0 51 199 40 535.2 0 40 200 49 535.2 0 49 201 46 535.2 0 46 202 46 535.2 0 46 203 52 535.2 0 52 204 72 535.2 0 72 205 70 535.2 0 70 206 134 535.2 0 134 207 94 535.2 0 94 208 78 535.2 0 78 209 44 535.2 0 44 210 249 535.2 0 249 211 192 535.2 0 192 212 243 535.2 0 243 213 89 535.2 0 89 214 56 535.2 0 56 215 43 535.2 0 43 216 39 535.2 0 39 217 54 535.2 0 54 218 44 535.2 0 44 219 80 535.2 0 80 220 206 535.2 0 206 221 104 535.2 0 104 222 52 535.2 0 52 223 40 535.2 0 40 224 34 535.2 0 34 225 59 535.2 0 59 226 49 535.2 0 49 227 52 535.2 0 52 228 59 535.2 0 59 229 73 535.2 0 73 230 288 535.2 0 288 231 516 535.2 0 516 232 368 535.2 0 368 233 53 535.2 0 53 234 28 535.2 0 28 235 32 535.2 0 32 236 32 535.2 0 32 237 33 535.2 0 33 238 24 535.2 0 24 239 57 535.2 0 57 240 56 535.2 0 56 241 56 535.2 0 56 242 43 535.2 0 43 243 36 535.2 0 36 244 37 535.2 0 37 245 34 535.2 0 34 246 38 535.2 0 38 247 23 535.2 0 23 248 31 535.2 0 31 249 34 535.2 0 34 250 34 535.2 0 34 251 35 535.2 0 35 252 50 535.2 0 50 253 56 535.2 0 56 254 78 535.2 0 78 255 77 535.2 0 77 256 45 535.2 0 45 257 48 535.2 0 48 258 50 535.2 0 50 259 34 535.2 0 34 260 46 535.2 0 46 261 67 535.2 0 67 262 34 535.2 0 34 263 97 535.2 0 97 264 66 535.2 0 66 265 53 535.2 0 53 266 50 535.2 0 50 267 39 535.2 0 39 268 74 535.2 0 74 269 73 535.2 0 73 270 44 535.2 0 44 271 39 535.2 0 39 272 58 535.2 0 58 273 79 535.2 0 79 274 80 535.2 0 80 275 192 535.2 0 192 276 202 535.2 0 202 277 243 535.2 0 243 278 111 535.2 0 111 279 154 535.2 0 154 280 147 535.2 0 147 281 83 535.2 0 83 282 55 535.2 0 55 283 76 535.2 0 76 284 67 535.2 0 67 285 80 535.2 0 80 286 40 535.2 0 40 287 63 535.2 0 63 288 165 535.2 0 165 289 274 535.2 0 274 290 157 535.2 0 157 291 178 535.2 0 178 292 36 535.2 0 36 293 57 535.2 0 57 294 78 535.2 0 78 295 124 535.2 0 124 296 78 535.2 0 78 297 106 535.2 0 106 298 40 535.2 0 40 299 63 535.2 0 63 300 171 535.2 0 171 301 147 535.2 0 147 Finished in 3.13 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 67,738 Reads with adapters: 24,669 (36.4%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 24,658 (36.4%) Total basepairs processed: 16,368,444 bp Total written (filtered): 6,681,206 bp (40.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24669 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 9 19 207 0.0 3 0 203 4 20 23856 0.0 4 23500 346 9 1 21 84 0.0 4 21 63 22 9 0.0 4 4 2 3 23 6 0.0 4 0 0 0 6 24 2 0.0 4 1 0 0 0 1 29 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 117 1 0.0 4 1 123 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 471 0.0 4 0 0 0 0 471 128 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.86 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 24,658 Reads with adapters: 2,279 (9.2%) Reads written (passing filters): 24,658 (100.0%) Total basepairs processed: 6,681,206 bp Total written (filtered): 6,654,700 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2279 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.3% G: 93.9% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 18 385.3 0 18 4 7 96.3 0 7 5 2 24.1 0 2 8 1 0.4 0 1 9 6 0.1 0 0 6 10 151 0.0 1 4 147 11 2071 0.0 1 14 2057 12 10 0.0 1 1 9 139 1 0.0 1 1 141 1 0.0 1 1 151 11 0.0 1 1 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.