This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.81 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 130,479 (6.0%) Reads that were too short: 30,942 (1.4%) Reads written (passing filters): 99,537 (4.5%) Total basepairs processed: 659,880,795 bp Total written (filtered): 22,869,374 bp (3.5%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 130479 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1963 535.2 0 1963 7 22119 535.2 0 22119 8 962 535.2 0 962 9 28828 535.2 0 28828 10 74 535.2 0 74 11 29 535.2 0 29 12 33 535.2 0 33 13 64 535.2 0 64 14 29 535.2 0 29 15 28 535.2 0 28 16 24 535.2 0 24 17 29 535.2 0 29 18 33 535.2 0 33 19 38 535.2 0 38 20 82 535.2 0 82 21 23 535.2 0 23 22 24 535.2 0 24 23 37 535.2 0 37 24 26 535.2 0 26 25 22 535.2 0 22 26 30 535.2 0 30 27 31 535.2 0 31 28 43 535.2 0 43 29 33 535.2 0 33 30 34 535.2 0 34 31 43 535.2 0 43 32 33 535.2 0 33 33 29 535.2 0 29 34 36 535.2 0 36 35 59 535.2 0 59 36 46 535.2 0 46 37 40 535.2 0 40 38 133 535.2 0 133 39 73 535.2 0 73 40 104 535.2 0 104 41 39 535.2 0 39 42 35 535.2 0 35 43 39 535.2 0 39 44 20 535.2 0 20 45 41 535.2 0 41 46 35 535.2 0 35 47 39 535.2 0 39 48 22 535.2 0 22 49 35 535.2 0 35 50 42 535.2 0 42 51 27 535.2 0 27 52 22 535.2 0 22 53 311 535.2 0 311 54 152 535.2 0 152 55 161 535.2 0 161 56 60 535.2 0 60 57 76 535.2 0 76 58 67 535.2 0 67 59 34 535.2 0 34 60 62 535.2 0 62 61 38 535.2 0 38 62 36 535.2 0 36 63 63 535.2 0 63 64 59 535.2 0 59 65 62 535.2 0 62 66 624 535.2 0 624 67 192 535.2 0 192 68 466 535.2 0 466 69 24 535.2 0 24 70 70 535.2 0 70 71 75 535.2 0 75 72 90 535.2 0 90 73 58 535.2 0 58 74 55 535.2 0 55 75 30 535.2 0 30 76 34 535.2 0 34 77 26 535.2 0 26 78 32 535.2 0 32 79 28 535.2 0 28 80 22 535.2 0 22 81 32 535.2 0 32 82 43 535.2 0 43 83 51 535.2 0 51 84 36 535.2 0 36 85 42 535.2 0 42 86 60 535.2 0 60 87 46 535.2 0 46 88 40 535.2 0 40 89 68 535.2 0 68 90 71 535.2 0 71 91 88 535.2 0 88 92 48 535.2 0 48 93 69 535.2 0 69 94 69 535.2 0 69 95 85 535.2 0 85 96 40 535.2 0 40 97 36 535.2 0 36 98 50 535.2 0 50 99 30 535.2 0 30 100 29 535.2 0 29 101 73 535.2 0 73 102 72 535.2 0 72 103 169 535.2 0 169 104 151 535.2 0 151 105 273 535.2 0 273 106 119 535.2 0 119 107 111 535.2 0 111 108 78 535.2 0 78 109 90 535.2 0 90 110 55 535.2 0 55 111 155 535.2 0 155 112 149 535.2 0 149 113 239 535.2 0 239 114 81 535.2 0 81 115 105 535.2 0 105 116 50 535.2 0 50 117 60 535.2 0 60 118 56 535.2 0 56 119 48 535.2 0 48 120 86 535.2 0 86 121 70 535.2 0 70 122 82 535.2 0 82 123 114 535.2 0 114 124 113 535.2 0 113 125 1144 535.2 0 1144 126 777 535.2 0 777 127 1248 535.2 0 1248 128 74 535.2 0 74 129 112 535.2 0 112 130 89 535.2 0 89 131 57 535.2 0 57 132 94 535.2 0 94 133 194 535.2 0 194 134 115 535.2 0 115 135 123 535.2 0 123 136 62 535.2 0 62 137 89 535.2 0 89 138 98 535.2 0 98 139 114 535.2 0 114 140 136 535.2 0 136 141 90 535.2 0 90 142 113 535.2 0 113 143 118 535.2 0 118 144 263 535.2 0 263 145 514 535.2 0 514 146 407 535.2 0 407 147 293 535.2 0 293 148 159 535.2 0 159 149 202 535.2 0 202 150 837 535.2 0 837 151 528 535.2 0 528 152 964 535.2 0 964 153 173 535.2 0 173 154 363 535.2 0 363 155 582 535.2 0 582 156 1421 535.2 0 1421 157 1040 535.2 0 1040 158 578 535.2 0 578 159 810 535.2 0 810 160 4084 535.2 0 4084 161 3031 535.2 0 3031 162 3064 535.2 0 3064 163 184 535.2 0 184 164 194 535.2 0 194 165 136 535.2 0 136 166 201 535.2 0 201 167 123 535.2 0 123 168 117 535.2 0 117 169 128 535.2 0 128 170 138 535.2 0 138 171 172 535.2 0 172 172 87 535.2 0 87 173 164 535.2 0 164 174 166 535.2 0 166 175 577 535.2 0 577 176 379 535.2 0 379 177 435 535.2 0 435 178 144 535.2 0 144 179 338 535.2 0 338 180 1401 535.2 0 1401 181 3085 535.2 0 3085 182 2354 535.2 0 2354 183 744 535.2 0 744 184 108 535.2 0 108 185 169 535.2 0 169 186 168 535.2 0 168 187 129 535.2 0 129 188 129 535.2 0 129 189 76 535.2 0 76 190 82 535.2 0 82 191 90 535.2 0 90 192 65 535.2 0 65 193 61 535.2 0 61 194 83 535.2 0 83 195 92 535.2 0 92 196 77 535.2 0 77 197 80 535.2 0 80 198 86 535.2 0 86 199 152 535.2 0 152 200 117 535.2 0 117 201 77 535.2 0 77 202 75 535.2 0 75 203 82 535.2 0 82 204 98 535.2 0 98 205 81 535.2 0 81 206 72 535.2 0 72 207 87 535.2 0 87 208 125 535.2 0 125 209 167 535.2 0 167 210 167 535.2 0 167 211 418 535.2 0 418 212 1601 535.2 0 1601 213 1284 535.2 0 1284 214 1368 535.2 0 1368 215 142 535.2 0 142 216 109 535.2 0 109 217 139 535.2 0 139 218 110 535.2 0 110 219 172 535.2 0 172 220 128 535.2 0 128 221 137 535.2 0 137 222 141 535.2 0 141 223 165 535.2 0 165 224 279 535.2 0 279 225 281 535.2 0 281 226 182 535.2 0 182 227 111 535.2 0 111 228 122 535.2 0 122 229 83 535.2 0 83 230 124 535.2 0 124 231 91 535.2 0 91 232 97 535.2 0 97 233 126 535.2 0 126 234 207 535.2 0 207 235 159 535.2 0 159 236 125 535.2 0 125 237 154 535.2 0 154 238 284 535.2 0 284 239 254 535.2 0 254 240 268 535.2 0 268 241 230 535.2 0 230 242 167 535.2 0 167 243 285 535.2 0 285 244 196 535.2 0 196 245 219 535.2 0 219 246 260 535.2 0 260 247 309 535.2 0 309 248 336 535.2 0 336 249 165 535.2 0 165 250 134 535.2 0 134 251 110 535.2 0 110 252 206 535.2 0 206 253 107 535.2 0 107 254 191 535.2 0 191 255 297 535.2 0 297 256 1266 535.2 0 1266 257 1011 535.2 0 1011 258 1168 535.2 0 1168 259 347 535.2 0 347 260 202 535.2 0 202 261 174 535.2 0 174 262 193 535.2 0 193 263 189 535.2 0 189 264 138 535.2 0 138 265 129 535.2 0 129 266 481 535.2 0 481 267 617 535.2 0 617 268 1102 535.2 0 1102 269 381 535.2 0 381 270 208 535.2 0 208 271 265 535.2 0 265 272 630 535.2 0 630 273 971 535.2 0 971 274 1119 535.2 0 1119 275 785 535.2 0 785 276 189 535.2 0 189 277 178 535.2 0 178 278 224 535.2 0 224 279 292 535.2 0 292 280 483 535.2 0 483 281 415 535.2 0 415 282 363 535.2 0 363 283 205 535.2 0 205 284 226 535.2 0 226 285 202 535.2 0 202 286 202 535.2 0 202 287 403 535.2 0 403 288 401 535.2 0 401 289 486 535.2 0 486 290 140 535.2 0 140 291 175 535.2 0 175 292 191 535.2 0 191 293 311 535.2 0 311 294 195 535.2 0 195 295 207 535.2 0 207 296 125 535.2 0 125 297 140 535.2 0 140 298 122 535.2 0 122 299 148 535.2 0 148 300 161 535.2 0 161 301 355 535.2 0 355 Finished in 4.17 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 99,537 Reads with adapters: 30,229 (30.4%) Reads that were too short: 183 (0.2%) Reads written (passing filters): 30,046 (30.2%) Total basepairs processed: 22,869,374 bp Total written (filtered): 8,251,544 bp (36.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30229 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 18 0.0 3 0 0 10 8 16 266 0.0 3 0 211 35 20 17 29029 0.0 3 26487 2006 433 103 18 464 0.0 3 347 94 17 6 19 94 0.0 3 6 1 45 42 20 82 0.0 3 0 0 0 82 21 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 25 2 0.0 3 1 0 0 1 27 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 1 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 0 4 34 2 0.0 3 0 0 0 2 35 13 0.0 3 0 0 1 12 36 1 0.0 3 0 0 0 1 37 13 0.0 3 0 0 1 12 38 3 0.0 3 1 0 0 2 39 3 0.0 3 0 0 0 3 41 3 0.0 3 0 0 0 3 46 4 0.0 3 0 0 0 4 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 0 4 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 61 4 0.0 3 0 0 0 4 62 10 0.0 3 0 0 1 9 64 5 0.0 3 0 0 0 5 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 8 0.0 3 0 0 0 8 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 1 0 0 2 94 1 0.0 3 0 0 0 1 97 1 0.0 3 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 1 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 0 4 113 14 0.0 3 0 0 0 14 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 4 0.0 3 0 0 0 4 123 5 0.0 3 0 0 0 5 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 5 0.0 3 0 0 0 5 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 167 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 175 3 0.0 3 0 0 1 2 183 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 30,046 Reads with adapters: 581 (1.9%) Reads written (passing filters): 30,046 (100.0%) Total basepairs processed: 8,251,544 bp Total written (filtered): 8,249,720 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 581 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.9% C: 13.4% G: 33.4% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 546 469.5 0 546 4 25 117.4 0 25 5 1 29.3 0 1 9 9 0.1 0 0 9