This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.34 s (22 us/read; 2.68 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 87,853 (2.8%) Reads that were too short: 38,448 (1.2%) Reads written (passing filters): 49,405 (1.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 8,892,468 bp (1.0%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 87853 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 63 755.5 0 63 7 69 755.5 0 69 8 39 755.5 0 39 9 363 755.5 0 363 10 134 755.5 0 134 11 7090 755.5 0 7090 12 15 755.5 0 15 13 6 755.5 0 6 14 5 755.5 0 5 15 10 755.5 0 10 16 7 755.5 0 7 17 7 755.5 0 7 18 5 755.5 0 5 19 8 755.5 0 8 20 6 755.5 0 6 21 15 755.5 0 15 22 7 755.5 0 7 23 7 755.5 0 7 24 8 755.5 0 8 25 11 755.5 0 11 26 10 755.5 0 10 27 5 755.5 0 5 28 14 755.5 0 14 29 18 755.5 0 18 30 18 755.5 0 18 31 5 755.5 0 5 32 12 755.5 0 12 33 5 755.5 0 5 34 7 755.5 0 7 35 11 755.5 0 11 36 11 755.5 0 11 37 10 755.5 0 10 38 14 755.5 0 14 39 20 755.5 0 20 40 13 755.5 0 13 41 11 755.5 0 11 42 17 755.5 0 17 43 4 755.5 0 4 44 14 755.5 0 14 45 13 755.5 0 13 46 6 755.5 0 6 47 6 755.5 0 6 48 11 755.5 0 11 49 9 755.5 0 9 50 4 755.5 0 4 51 5 755.5 0 5 52 20 755.5 0 20 53 27 755.5 0 27 54 18 755.5 0 18 55 27 755.5 0 27 56 10 755.5 0 10 57 6 755.5 0 6 58 11 755.5 0 11 59 8 755.5 0 8 60 9 755.5 0 9 61 12 755.5 0 12 62 4 755.5 0 4 63 10 755.5 0 10 64 15 755.5 0 15 65 11 755.5 0 11 66 8 755.5 0 8 67 14 755.5 0 14 68 12 755.5 0 12 69 24 755.5 0 24 70 44 755.5 0 44 71 1038 755.5 0 1038 72 64 755.5 0 64 73 97 755.5 0 97 74 272 755.5 0 272 75 86 755.5 0 86 76 59 755.5 0 59 77 32 755.5 0 32 78 53 755.5 0 53 79 85 755.5 0 85 80 337 755.5 0 337 81 100 755.5 0 100 82 203 755.5 0 203 83 112 755.5 0 112 84 325 755.5 0 325 85 78 755.5 0 78 86 98 755.5 0 98 87 128 755.5 0 128 88 60 755.5 0 60 89 63 755.5 0 63 90 143 755.5 0 143 91 98 755.5 0 98 92 298 755.5 0 298 93 160 755.5 0 160 94 43 755.5 0 43 95 51 755.5 0 51 96 43 755.5 0 43 97 98 755.5 0 98 98 227 755.5 0 227 99 260 755.5 0 260 100 338 755.5 0 338 101 1041 755.5 0 1041 102 514 755.5 0 514 103 854 755.5 0 854 104 463 755.5 0 463 105 72 755.5 0 72 106 97 755.5 0 97 107 52 755.5 0 52 108 52 755.5 0 52 109 139 755.5 0 139 110 144 755.5 0 144 111 204 755.5 0 204 112 167 755.5 0 167 113 268 755.5 0 268 114 153 755.5 0 153 115 294 755.5 0 294 116 402 755.5 0 402 117 395 755.5 0 395 118 307 755.5 0 307 119 204 755.5 0 204 120 190 755.5 0 190 121 147 755.5 0 147 122 128 755.5 0 128 123 484 755.5 0 484 124 387 755.5 0 387 125 393 755.5 0 393 126 174 755.5 0 174 127 70 755.5 0 70 128 63 755.5 0 63 129 95 755.5 0 95 130 621 755.5 0 621 131 629 755.5 0 629 132 825 755.5 0 825 133 468 755.5 0 468 134 249 755.5 0 249 135 200 755.5 0 200 136 337 755.5 0 337 137 488 755.5 0 488 138 507 755.5 0 507 139 316 755.5 0 316 140 118 755.5 0 118 141 243 755.5 0 243 142 597 755.5 0 597 143 657 755.5 0 657 144 767 755.5 0 767 145 352 755.5 0 352 146 288 755.5 0 288 147 145 755.5 0 145 148 253 755.5 0 253 149 601 755.5 0 601 150 315 755.5 0 315 151 513 755.5 0 513 152 343 755.5 0 343 153 236 755.5 0 236 154 429 755.5 0 429 155 347 755.5 0 347 156 557 755.5 0 557 157 909 755.5 0 909 158 900 755.5 0 900 159 375 755.5 0 375 160 265 755.5 0 265 161 191 755.5 0 191 162 257 755.5 0 257 163 268 755.5 0 268 164 395 755.5 0 395 165 218 755.5 0 218 166 228 755.5 0 228 167 207 755.5 0 207 168 300 755.5 0 300 169 552 755.5 0 552 170 1033 755.5 0 1033 171 1120 755.5 0 1120 172 322 755.5 0 322 173 138 755.5 0 138 174 51 755.5 0 51 175 111 755.5 0 111 176 260 755.5 0 260 177 845 755.5 0 845 178 764 755.5 0 764 179 726 755.5 0 726 180 402 755.5 0 402 181 136 755.5 0 136 182 198 755.5 0 198 183 223 755.5 0 223 184 257 755.5 0 257 185 205 755.5 0 205 186 129 755.5 0 129 187 312 755.5 0 312 188 662 755.5 0 662 189 712 755.5 0 712 190 225 755.5 0 225 191 122 755.5 0 122 192 105 755.5 0 105 193 94 755.5 0 94 194 104 755.5 0 104 195 408 755.5 0 408 196 275 755.5 0 275 197 356 755.5 0 356 198 267 755.5 0 267 199 258 755.5 0 258 200 318 755.5 0 318 201 361 755.5 0 361 202 323 755.5 0 323 203 297 755.5 0 297 204 263 755.5 0 263 205 299 755.5 0 299 206 290 755.5 0 290 207 405 755.5 0 405 208 308 755.5 0 308 209 440 755.5 0 440 210 578 755.5 0 578 211 466 755.5 0 466 212 346 755.5 0 346 213 264 755.5 0 264 214 340 755.5 0 340 215 435 755.5 0 435 216 464 755.5 0 464 217 377 755.5 0 377 218 210 755.5 0 210 219 154 755.5 0 154 220 161 755.5 0 161 221 349 755.5 0 349 222 465 755.5 0 465 223 528 755.5 0 528 224 366 755.5 0 366 225 398 755.5 0 398 226 404 755.5 0 404 227 261 755.5 0 261 228 192 755.5 0 192 229 261 755.5 0 261 230 127 755.5 0 127 231 167 755.5 0 167 232 198 755.5 0 198 233 250 755.5 0 250 234 186 755.5 0 186 235 136 755.5 0 136 236 181 755.5 0 181 237 210 755.5 0 210 238 206 755.5 0 206 239 243 755.5 0 243 240 193 755.5 0 193 241 311 755.5 0 311 242 572 755.5 0 572 243 568 755.5 0 568 244 453 755.5 0 453 245 424 755.5 0 424 246 242 755.5 0 242 247 269 755.5 0 269 248 347 755.5 0 347 249 409 755.5 0 409 250 507 755.5 0 507 251 468 755.5 0 468 252 723 755.5 0 723 253 504 755.5 0 504 254 238 755.5 0 238 255 139 755.5 0 139 256 134 755.5 0 134 257 203 755.5 0 203 258 190 755.5 0 190 259 303 755.5 0 303 260 287 755.5 0 287 261 488 755.5 0 488 262 334 755.5 0 334 263 550 755.5 0 550 264 875 755.5 0 875 265 431 755.5 0 431 266 171 755.5 0 171 267 153 755.5 0 153 268 198 755.5 0 198 269 255 755.5 0 255 270 607 755.5 0 607 271 594 755.5 0 594 272 655 755.5 0 655 273 442 755.5 0 442 274 371 755.5 0 371 275 259 755.5 0 259 276 402 755.5 0 402 277 574 755.5 0 574 278 327 755.5 0 327 279 159 755.5 0 159 280 170 755.5 0 170 281 332 755.5 0 332 282 526 755.5 0 526 283 1373 755.5 0 1373 284 2910 755.5 0 2910 285 852 755.5 0 852 286 404 755.5 0 404 287 168 755.5 0 168 288 224 755.5 0 224 289 278 755.5 0 278 290 509 755.5 0 509 291 532 755.5 0 532 292 296 755.5 0 296 293 293 755.5 0 293 294 186 755.5 0 186 295 211 755.5 0 211 296 363 755.5 0 363 297 393 755.5 0 393 298 211 755.5 0 211 299 340 755.5 0 340 300 643 755.5 0 643 301 357 755.5 0 357 Finished in 1.42 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 49,405 Reads with adapters: 409 (0.8%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 387 (0.8%) Total basepairs processed: 8,892,468 bp Total written (filtered): 95,888 bp (1.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 409 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 0 0 3 17 309 0.0 3 298 9 1 1 18 4 0.0 3 0 2 1 1 19 1 0.0 3 0 0 0 1 20 2 0.0 3 1 0 0 1 21 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 63 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 1 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 162 17 0.0 3 0 0 0 17 163 15 0.0 3 0 0 0 15 165 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 181 7 0.0 3 0 0 0 7 182 6 0.0 3 0 0 0 6 183 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 274 3 0.0 3 0 0 0 3 275 3 0.0 3 0 0 0 3 276 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 1 2 279 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 387 Reads with adapters: 22 (5.7%) Reads written (passing filters): 387 (100.0%) Total basepairs processed: 95,888 bp Total written (filtered): 95,815 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 22 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.1% C: 9.1% G: 4.5% T: 27.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 15 6.0 0 15 4 7 1.5 0 7