This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.57 s (22 us/read; 2.67 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 209,942 (6.8%) Reads that were too short: 28,966 (0.9%) Reads written (passing filters): 180,976 (5.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 37,602,960 bp (4.0%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 209942 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 24 755.5 0 24 7 14 755.5 0 14 8 180 755.5 0 180 9 14 755.5 0 14 10 5 755.5 0 5 11 47 755.5 0 47 12 31 755.5 0 31 13 5 755.5 0 5 14 10 755.5 0 10 15 6 755.5 0 6 16 8 755.5 0 8 17 13 755.5 0 13 18 9 755.5 0 9 19 8 755.5 0 8 20 9 755.5 0 9 21 12 755.5 0 12 22 16 755.5 0 16 23 9 755.5 0 9 24 10 755.5 0 10 25 22 755.5 0 22 26 17 755.5 0 17 27 8 755.5 0 8 28 9 755.5 0 9 29 5 755.5 0 5 30 9 755.5 0 9 31 10 755.5 0 10 32 8 755.5 0 8 33 14 755.5 0 14 34 9 755.5 0 9 35 14 755.5 0 14 36 7 755.5 0 7 37 6 755.5 0 6 38 22 755.5 0 22 39 24 755.5 0 24 40 6 755.5 0 6 41 11 755.5 0 11 42 9 755.5 0 9 43 10 755.5 0 10 44 10 755.5 0 10 45 24 755.5 0 24 46 12 755.5 0 12 47 14 755.5 0 14 48 10 755.5 0 10 49 14 755.5 0 14 50 14 755.5 0 14 51 74 755.5 0 74 52 15 755.5 0 15 53 8 755.5 0 8 54 45 755.5 0 45 55 20 755.5 0 20 56 26 755.5 0 26 57 74 755.5 0 74 58 33 755.5 0 33 59 26 755.5 0 26 60 27 755.5 0 27 61 199 755.5 0 199 62 24 755.5 0 24 63 43 755.5 0 43 64 20 755.5 0 20 65 36 755.5 0 36 66 10 755.5 0 10 67 24 755.5 0 24 68 17 755.5 0 17 69 56 755.5 0 56 70 19 755.5 0 19 71 41 755.5 0 41 72 61 755.5 0 61 73 94 755.5 0 94 74 145 755.5 0 145 75 243 755.5 0 243 76 656 755.5 0 656 77 110 755.5 0 110 78 181 755.5 0 181 79 416 755.5 0 416 80 258 755.5 0 258 81 250 755.5 0 250 82 454 755.5 0 454 83 1088 755.5 0 1088 84 607 755.5 0 607 85 903 755.5 0 903 86 1077 755.5 0 1077 87 1076 755.5 0 1076 88 1344 755.5 0 1344 89 2425 755.5 0 2425 90 13015 755.5 0 13015 91 145280 755.5 0 145280 92 592 755.5 0 592 93 30 755.5 0 30 94 97 755.5 0 97 95 46 755.5 0 46 96 55 755.5 0 55 97 87 755.5 0 87 98 61 755.5 0 61 99 79 755.5 0 79 100 91 755.5 0 91 101 71 755.5 0 71 102 151 755.5 0 151 103 12 755.5 0 12 104 7 755.5 0 7 105 25 755.5 0 25 106 12 755.5 0 12 107 10 755.5 0 10 108 11 755.5 0 11 109 7 755.5 0 7 110 12 755.5 0 12 111 19 755.5 0 19 112 223 755.5 0 223 113 17 755.5 0 17 114 20 755.5 0 20 115 9 755.5 0 9 116 14 755.5 0 14 117 11 755.5 0 11 118 15 755.5 0 15 119 20 755.5 0 20 120 48 755.5 0 48 121 26 755.5 0 26 122 35 755.5 0 35 123 11 755.5 0 11 124 9 755.5 0 9 125 16 755.5 0 16 126 28 755.5 0 28 127 27 755.5 0 27 128 12 755.5 0 12 129 21 755.5 0 21 130 28 755.5 0 28 131 38 755.5 0 38 132 331 755.5 0 331 133 60 755.5 0 60 134 30 755.5 0 30 135 57 755.5 0 57 136 19 755.5 0 19 137 32 755.5 0 32 138 30 755.5 0 30 139 22 755.5 0 22 140 67 755.5 0 67 141 38 755.5 0 38 142 30 755.5 0 30 143 15 755.5 0 15 144 12 755.5 0 12 145 31 755.5 0 31 146 33 755.5 0 33 147 51 755.5 0 51 148 300 755.5 0 300 149 708 755.5 0 708 150 1320 755.5 0 1320 151 14 755.5 0 14 152 12 755.5 0 12 153 21 755.5 0 21 154 26 755.5 0 26 155 25 755.5 0 25 156 26 755.5 0 26 157 31 755.5 0 31 158 31 755.5 0 31 159 57 755.5 0 57 160 37 755.5 0 37 161 26 755.5 0 26 162 27 755.5 0 27 163 14 755.5 0 14 164 34 755.5 0 34 165 66 755.5 0 66 166 59 755.5 0 59 167 61 755.5 0 61 168 646 755.5 0 646 169 912 755.5 0 912 170 82 755.5 0 82 171 16 755.5 0 16 172 27 755.5 0 27 173 45 755.5 0 45 174 33 755.5 0 33 175 70 755.5 0 70 176 609 755.5 0 609 177 157 755.5 0 157 178 548 755.5 0 548 179 50 755.5 0 50 180 25 755.5 0 25 181 26 755.5 0 26 182 16 755.5 0 16 183 34 755.5 0 34 184 55 755.5 0 55 185 56 755.5 0 56 186 68 755.5 0 68 187 21 755.5 0 21 188 9 755.5 0 9 189 4 755.5 0 4 190 14 755.5 0 14 191 21 755.5 0 21 192 21 755.5 0 21 193 68 755.5 0 68 194 60 755.5 0 60 195 18 755.5 0 18 196 17 755.5 0 17 197 20 755.5 0 20 198 32 755.5 0 32 199 36 755.5 0 36 200 17 755.5 0 17 201 19 755.5 0 19 202 18 755.5 0 18 203 47 755.5 0 47 204 56 755.5 0 56 205 106 755.5 0 106 206 104 755.5 0 104 207 275 755.5 0 275 208 128 755.5 0 128 209 91 755.5 0 91 210 89 755.5 0 89 211 42 755.5 0 42 212 67 755.5 0 67 213 89 755.5 0 89 214 76 755.5 0 76 215 114 755.5 0 114 216 135 755.5 0 135 217 81 755.5 0 81 218 127 755.5 0 127 219 261 755.5 0 261 220 1662 755.5 0 1662 221 792 755.5 0 792 222 1902 755.5 0 1902 223 42 755.5 0 42 224 37 755.5 0 37 225 83 755.5 0 83 226 81 755.5 0 81 227 71 755.5 0 71 228 86 755.5 0 86 229 45 755.5 0 45 230 66 755.5 0 66 231 50 755.5 0 50 232 61 755.5 0 61 233 25 755.5 0 25 234 29 755.5 0 29 235 70 755.5 0 70 236 63 755.5 0 63 237 81 755.5 0 81 238 35 755.5 0 35 239 51 755.5 0 51 240 63 755.5 0 63 241 132 755.5 0 132 242 54 755.5 0 54 243 48 755.5 0 48 244 50 755.5 0 50 245 76 755.5 0 76 246 285 755.5 0 285 247 61 755.5 0 61 248 29 755.5 0 29 249 19 755.5 0 19 250 23 755.5 0 23 251 40 755.5 0 40 252 32 755.5 0 32 253 30 755.5 0 30 254 71 755.5 0 71 255 245 755.5 0 245 256 236 755.5 0 236 257 60 755.5 0 60 258 82 755.5 0 82 259 125 755.5 0 125 260 34 755.5 0 34 261 46 755.5 0 46 262 34 755.5 0 34 263 36 755.5 0 36 264 58 755.5 0 58 265 145 755.5 0 145 266 211 755.5 0 211 267 147 755.5 0 147 268 246 755.5 0 246 269 304 755.5 0 304 270 926 755.5 0 926 271 1287 755.5 0 1287 272 215 755.5 0 215 273 60 755.5 0 60 274 393 755.5 0 393 275 121 755.5 0 121 276 853 755.5 0 853 277 37 755.5 0 37 278 58 755.5 0 58 279 180 755.5 0 180 280 291 755.5 0 291 281 475 755.5 0 475 282 829 755.5 0 829 283 6015 755.5 0 6015 284 1690 755.5 0 1690 285 2670 755.5 0 2670 286 568 755.5 0 568 287 45 755.5 0 45 288 44 755.5 0 44 289 191 755.5 0 191 290 217 755.5 0 217 291 130 755.5 0 130 292 63 755.5 0 63 293 231 755.5 0 231 294 319 755.5 0 319 295 97 755.5 0 97 296 328 755.5 0 328 297 47 755.5 0 47 298 54 755.5 0 54 299 137 755.5 0 137 300 100 755.5 0 100 301 35 755.5 0 35 Finished in 5.54 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 180,976 Reads with adapters: 1,247 (0.7%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 1,175 (0.6%) Total basepairs processed: 37,602,960 bp Total written (filtered): 157,192 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1247 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 2 17 160 0.0 3 156 4 18 6 0.0 3 0 0 6 19 4 0.0 3 0 1 1 2 20 5 0.0 3 2 1 0 2 29 1 0.0 3 0 0 0 1 43 19 0.0 3 0 0 0 19 45 1 0.0 3 0 0 0 1 46 10 0.0 3 0 0 0 10 48 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 19 0.0 3 0 0 0 19 102 824 0.0 3 0 0 1 823 103 10 0.0 3 0 0 0 10 104 1 0.0 3 0 0 0 1 105 107 0.0 3 0 0 1 106 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 203 36 0.0 3 0 0 1 35 204 24 0.0 3 0 0 0 24 205 2 0.0 3 0 0 0 2 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 1,175 Reads with adapters: 12 (1.0%) Reads written (passing filters): 1,175 (100.0%) Total basepairs processed: 157,192 bp Total written (filtered): 157,155 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 16.7% G: 41.7% T: 8.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 18.4 0 11 4 1 4.6 0 1