This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.66 s (22 us/read; 2.74 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 29,929 (1.0%) Reads that were too short: 5,718 (0.2%) Reads written (passing filters): 24,211 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 6,396,224 bp (0.7%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 29929 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 155 755.5 0 155 7 275 755.5 0 275 8 18054 755.5 0 18054 9 209 755.5 0 209 10 5 755.5 0 5 11 3 755.5 0 3 12 3 755.5 0 3 13 3 755.5 0 3 14 4 755.5 0 4 15 4 755.5 0 4 16 5 755.5 0 5 17 2 755.5 0 2 18 1 755.5 0 1 19 6 755.5 0 6 20 9 755.5 0 9 21 6 755.5 0 6 22 5 755.5 0 5 23 7 755.5 0 7 24 5 755.5 0 5 25 4 755.5 0 4 26 10 755.5 0 10 27 3 755.5 0 3 28 5 755.5 0 5 29 9 755.5 0 9 30 8 755.5 0 8 31 4 755.5 0 4 32 5 755.5 0 5 33 7 755.5 0 7 34 5 755.5 0 5 35 2 755.5 0 2 36 6 755.5 0 6 37 8 755.5 0 8 38 49 755.5 0 49 39 9 755.5 0 9 40 12 755.5 0 12 41 4 755.5 0 4 42 6 755.5 0 6 43 10 755.5 0 10 44 10 755.5 0 10 45 5 755.5 0 5 46 17 755.5 0 17 47 10 755.5 0 10 48 5 755.5 0 5 49 5 755.5 0 5 50 9 755.5 0 9 51 1 755.5 0 1 52 10 755.5 0 10 53 14 755.5 0 14 54 70 755.5 0 70 55 7 755.5 0 7 56 10 755.5 0 10 57 3 755.5 0 3 58 7 755.5 0 7 59 10 755.5 0 10 60 14 755.5 0 14 61 8 755.5 0 8 62 16 755.5 0 16 63 16 755.5 0 16 64 6 755.5 0 6 65 16 755.5 0 16 66 16 755.5 0 16 67 25 755.5 0 25 68 14 755.5 0 14 69 5 755.5 0 5 70 8 755.5 0 8 71 9 755.5 0 9 72 10 755.5 0 10 73 5 755.5 0 5 74 14 755.5 0 14 75 12 755.5 0 12 76 10 755.5 0 10 77 8 755.5 0 8 78 9 755.5 0 9 79 7 755.5 0 7 80 21 755.5 0 21 81 8 755.5 0 8 82 5 755.5 0 5 83 13 755.5 0 13 84 12 755.5 0 12 85 9 755.5 0 9 86 4 755.5 0 4 87 9 755.5 0 9 88 11 755.5 0 11 89 10 755.5 0 10 90 9 755.5 0 9 91 31 755.5 0 31 92 11 755.5 0 11 93 13 755.5 0 13 94 22 755.5 0 22 95 185 755.5 0 185 96 41 755.5 0 41 97 107 755.5 0 107 98 97 755.5 0 97 99 133 755.5 0 133 100 117 755.5 0 117 101 19 755.5 0 19 102 31 755.5 0 31 103 10 755.5 0 10 104 10 755.5 0 10 105 9 755.5 0 9 106 14 755.5 0 14 107 12 755.5 0 12 108 28 755.5 0 28 109 22 755.5 0 22 110 11 755.5 0 11 111 55 755.5 0 55 112 64 755.5 0 64 113 373 755.5 0 373 114 6 755.5 0 6 115 10 755.5 0 10 116 18 755.5 0 18 117 18 755.5 0 18 118 12 755.5 0 12 119 8 755.5 0 8 120 27 755.5 0 27 121 24 755.5 0 24 122 17 755.5 0 17 123 13 755.5 0 13 124 12 755.5 0 12 125 14 755.5 0 14 126 20 755.5 0 20 127 22 755.5 0 22 128 13 755.5 0 13 129 59 755.5 0 59 130 380 755.5 0 380 131 23 755.5 0 23 132 43 755.5 0 43 133 38 755.5 0 38 134 83 755.5 0 83 135 33 755.5 0 33 136 46 755.5 0 46 137 21 755.5 0 21 138 25 755.5 0 25 139 11 755.5 0 11 140 46 755.5 0 46 141 9 755.5 0 9 142 11 755.5 0 11 143 7 755.5 0 7 144 9 755.5 0 9 145 15 755.5 0 15 146 27 755.5 0 27 147 25 755.5 0 25 148 17 755.5 0 17 149 7 755.5 0 7 150 10 755.5 0 10 151 9 755.5 0 9 152 7 755.5 0 7 153 12 755.5 0 12 154 5 755.5 0 5 155 13 755.5 0 13 156 55 755.5 0 55 157 25 755.5 0 25 158 18 755.5 0 18 159 21 755.5 0 21 160 15 755.5 0 15 161 19 755.5 0 19 162 13 755.5 0 13 163 4 755.5 0 4 164 24 755.5 0 24 165 16 755.5 0 16 166 56 755.5 0 56 167 36 755.5 0 36 168 34 755.5 0 34 169 31 755.5 0 31 170 6 755.5 0 6 171 21 755.5 0 21 172 22 755.5 0 22 173 40 755.5 0 40 174 27 755.5 0 27 175 38 755.5 0 38 176 41 755.5 0 41 177 7 755.5 0 7 178 11 755.5 0 11 179 25 755.5 0 25 180 40 755.5 0 40 181 102 755.5 0 102 182 47 755.5 0 47 183 24 755.5 0 24 184 28 755.5 0 28 185 47 755.5 0 47 186 61 755.5 0 61 187 27 755.5 0 27 188 9 755.5 0 9 189 13 755.5 0 13 190 11 755.5 0 11 191 16 755.5 0 16 192 46 755.5 0 46 193 293 755.5 0 293 194 162 755.5 0 162 195 78 755.5 0 78 196 137 755.5 0 137 197 55 755.5 0 55 198 24 755.5 0 24 199 92 755.5 0 92 200 82 755.5 0 82 201 113 755.5 0 113 202 85 755.5 0 85 203 17 755.5 0 17 204 29 755.5 0 29 205 34 755.5 0 34 206 36 755.5 0 36 207 32 755.5 0 32 208 35 755.5 0 35 209 20 755.5 0 20 210 21 755.5 0 21 211 27 755.5 0 27 212 42 755.5 0 42 213 37 755.5 0 37 214 28 755.5 0 28 215 64 755.5 0 64 216 71 755.5 0 71 217 100 755.5 0 100 218 35 755.5 0 35 219 45 755.5 0 45 220 104 755.5 0 104 221 166 755.5 0 166 222 358 755.5 0 358 223 86 755.5 0 86 224 22 755.5 0 22 225 27 755.5 0 27 226 34 755.5 0 34 227 42 755.5 0 42 228 53 755.5 0 53 229 28 755.5 0 28 230 28 755.5 0 28 231 17 755.5 0 17 232 19 755.5 0 19 233 13 755.5 0 13 234 20 755.5 0 20 235 23 755.5 0 23 236 35 755.5 0 35 237 63 755.5 0 63 238 132 755.5 0 132 239 73 755.5 0 73 240 12 755.5 0 12 241 17 755.5 0 17 242 41 755.5 0 41 243 57 755.5 0 57 244 39 755.5 0 39 245 39 755.5 0 39 246 32 755.5 0 32 247 30 755.5 0 30 248 36 755.5 0 36 249 24 755.5 0 24 250 9 755.5 0 9 251 21 755.5 0 21 252 33 755.5 0 33 253 52 755.5 0 52 254 83 755.5 0 83 255 145 755.5 0 145 256 694 755.5 0 694 257 83 755.5 0 83 258 51 755.5 0 51 259 27 755.5 0 27 260 11 755.5 0 11 261 25 755.5 0 25 262 124 755.5 0 124 263 580 755.5 0 580 264 481 755.5 0 481 265 44 755.5 0 44 266 44 755.5 0 44 267 47 755.5 0 47 268 28 755.5 0 28 269 52 755.5 0 52 270 27 755.5 0 27 271 11 755.5 0 11 272 18 755.5 0 18 273 21 755.5 0 21 274 20 755.5 0 20 275 32 755.5 0 32 276 20 755.5 0 20 277 11 755.5 0 11 278 16 755.5 0 16 279 32 755.5 0 32 280 46 755.5 0 46 281 41 755.5 0 41 282 16 755.5 0 16 283 16 755.5 0 16 284 12 755.5 0 12 285 32 755.5 0 32 286 33 755.5 0 33 287 53 755.5 0 53 288 21 755.5 0 21 289 16 755.5 0 16 290 6 755.5 0 6 291 17 755.5 0 17 292 21 755.5 0 21 293 19 755.5 0 19 294 16 755.5 0 16 295 17 755.5 0 17 296 4 755.5 0 4 297 10 755.5 0 10 298 4 755.5 0 4 299 8 755.5 0 8 300 10 755.5 0 10 301 30 755.5 0 30 Finished in 1.07 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 24,211 Reads with adapters: 237 (1.0%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 233 (1.0%) Total basepairs processed: 6,396,224 bp Total written (filtered): 58,535 bp (0.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 237 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 2 0.0 3 0 0 2 19 12 0.0 3 0 12 20 157 0.0 4 156 1 22 1 0.0 4 0 0 0 0 1 23 12 0.0 4 1 0 0 11 24 5 0.0 4 0 0 0 0 5 127 27 0.0 4 0 0 0 0 27 128 1 0.0 4 0 0 0 0 1 153 14 0.0 4 0 0 0 0 14 156 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 246 1 0.0 4 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 233 Reads with adapters: 28 (12.0%) Reads written (passing filters): 233 (100.0%) Total basepairs processed: 58,535 bp Total written (filtered): 52,352 bp (89.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.1% C: 71.4% G: 7.1% T: 10.7% none/other: 3.6% Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 1 0.0 1 0 1 223 15 0.0 1 13 2 224 2 0.0 1 2 256 1 0.0 1 1 257 2 0.0 1 2 263 2 0.0 1 2 267 3 0.0 1 3 272 1 0.0 1 1