This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.46 s (30 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 72,076 (2.3%) Reads that were too short: 18,215 (0.6%) Reads written (passing filters): 53,861 (1.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 11,357,210 bp (1.2%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 72076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3564 755.5 0 3564 7 10870 755.5 0 10870 8 32 755.5 0 32 9 13 755.5 0 13 10 17 755.5 0 17 11 14 755.5 0 14 12 11 755.5 0 11 13 10 755.5 0 10 14 13 755.5 0 13 15 16 755.5 0 16 16 14 755.5 0 14 17 16 755.5 0 16 18 22 755.5 0 22 19 11 755.5 0 11 20 106 755.5 0 106 21 14 755.5 0 14 22 21 755.5 0 21 23 9 755.5 0 9 24 11 755.5 0 11 25 12 755.5 0 12 26 12 755.5 0 12 27 9 755.5 0 9 28 19 755.5 0 19 29 29 755.5 0 29 30 30 755.5 0 30 31 16 755.5 0 16 32 6 755.5 0 6 33 14 755.5 0 14 34 13 755.5 0 13 35 22 755.5 0 22 36 19 755.5 0 19 37 15 755.5 0 15 38 17 755.5 0 17 39 12 755.5 0 12 40 16 755.5 0 16 41 11 755.5 0 11 42 23 755.5 0 23 43 14 755.5 0 14 44 30 755.5 0 30 45 15 755.5 0 15 46 12 755.5 0 12 47 20 755.5 0 20 48 16 755.5 0 16 49 18 755.5 0 18 50 18 755.5 0 18 51 23 755.5 0 23 52 12 755.5 0 12 53 29 755.5 0 29 54 22 755.5 0 22 55 21 755.5 0 21 56 23 755.5 0 23 57 15 755.5 0 15 58 24 755.5 0 24 59 14 755.5 0 14 60 27 755.5 0 27 61 19 755.5 0 19 62 19 755.5 0 19 63 20 755.5 0 20 64 30 755.5 0 30 65 37 755.5 0 37 66 70 755.5 0 70 67 157 755.5 0 157 68 97 755.5 0 97 69 120 755.5 0 120 70 768 755.5 0 768 71 324 755.5 0 324 72 55 755.5 0 55 73 87 755.5 0 87 74 84 755.5 0 84 75 125 755.5 0 125 76 104 755.5 0 104 77 141 755.5 0 141 78 206 755.5 0 206 79 313 755.5 0 313 80 2690 755.5 0 2690 81 5743 755.5 0 5743 82 9088 755.5 0 9088 83 259 755.5 0 259 84 134 755.5 0 134 85 87 755.5 0 87 86 97 755.5 0 97 87 129 755.5 0 129 88 31 755.5 0 31 89 66 755.5 0 66 90 55 755.5 0 55 91 37 755.5 0 37 92 43 755.5 0 43 93 76 755.5 0 76 94 70 755.5 0 70 95 67 755.5 0 67 96 101 755.5 0 101 97 125 755.5 0 125 98 24 755.5 0 24 99 23 755.5 0 23 100 17 755.5 0 17 101 24 755.5 0 24 102 15 755.5 0 15 103 21 755.5 0 21 104 17 755.5 0 17 105 6 755.5 0 6 106 7 755.5 0 7 107 20 755.5 0 20 108 13 755.5 0 13 109 11 755.5 0 11 110 25 755.5 0 25 111 22 755.5 0 22 112 20 755.5 0 20 113 30 755.5 0 30 114 20 755.5 0 20 115 20 755.5 0 20 116 63 755.5 0 63 117 95 755.5 0 95 118 98 755.5 0 98 119 240 755.5 0 240 120 64 755.5 0 64 121 87 755.5 0 87 122 71 755.5 0 71 123 39 755.5 0 39 124 56 755.5 0 56 125 70 755.5 0 70 126 61 755.5 0 61 127 238 755.5 0 238 128 45 755.5 0 45 129 62 755.5 0 62 130 16 755.5 0 16 131 49 755.5 0 49 132 42 755.5 0 42 133 58 755.5 0 58 134 47 755.5 0 47 135 38 755.5 0 38 136 51 755.5 0 51 137 45 755.5 0 45 138 45 755.5 0 45 139 64 755.5 0 64 140 43 755.5 0 43 141 55 755.5 0 55 142 118 755.5 0 118 143 61 755.5 0 61 144 41 755.5 0 41 145 151 755.5 0 151 146 61 755.5 0 61 147 53 755.5 0 53 148 48 755.5 0 48 149 34 755.5 0 34 150 68 755.5 0 68 151 33 755.5 0 33 152 49 755.5 0 49 153 44 755.5 0 44 154 36 755.5 0 36 155 56 755.5 0 56 156 38 755.5 0 38 157 52 755.5 0 52 158 59 755.5 0 59 159 61 755.5 0 61 160 57 755.5 0 57 161 62 755.5 0 62 162 58 755.5 0 58 163 39 755.5 0 39 164 43 755.5 0 43 165 55 755.5 0 55 166 101 755.5 0 101 167 117 755.5 0 117 168 100 755.5 0 100 169 43 755.5 0 43 170 48 755.5 0 48 171 86 755.5 0 86 172 44 755.5 0 44 173 37 755.5 0 37 174 53 755.5 0 53 175 104 755.5 0 104 176 66 755.5 0 66 177 73 755.5 0 73 178 100 755.5 0 100 179 58 755.5 0 58 180 161 755.5 0 161 181 86 755.5 0 86 182 90 755.5 0 90 183 104 755.5 0 104 184 78 755.5 0 78 185 94 755.5 0 94 186 80 755.5 0 80 187 175 755.5 0 175 188 1242 755.5 0 1242 189 1413 755.5 0 1413 190 4502 755.5 0 4502 191 440 755.5 0 440 192 375 755.5 0 375 193 1013 755.5 0 1013 194 922 755.5 0 922 195 788 755.5 0 788 196 303 755.5 0 303 197 27 755.5 0 27 198 42 755.5 0 42 199 38 755.5 0 38 200 77 755.5 0 77 201 121 755.5 0 121 202 38 755.5 0 38 203 60 755.5 0 60 204 64 755.5 0 64 205 66 755.5 0 66 206 145 755.5 0 145 207 287 755.5 0 287 208 416 755.5 0 416 209 315 755.5 0 315 210 75 755.5 0 75 211 144 755.5 0 144 212 537 755.5 0 537 213 416 755.5 0 416 214 252 755.5 0 252 215 91 755.5 0 91 216 53 755.5 0 53 217 52 755.5 0 52 218 47 755.5 0 47 219 42 755.5 0 42 220 53 755.5 0 53 221 96 755.5 0 96 222 58 755.5 0 58 223 48 755.5 0 48 224 76 755.5 0 76 225 66 755.5 0 66 226 45 755.5 0 45 227 47 755.5 0 47 228 52 755.5 0 52 229 45 755.5 0 45 230 42 755.5 0 42 231 82 755.5 0 82 232 129 755.5 0 129 233 54 755.5 0 54 234 39 755.5 0 39 235 89 755.5 0 89 236 59 755.5 0 59 237 115 755.5 0 115 238 160 755.5 0 160 239 52 755.5 0 52 240 153 755.5 0 153 241 153 755.5 0 153 242 97 755.5 0 97 243 28 755.5 0 28 244 64 755.5 0 64 245 53 755.5 0 53 246 35 755.5 0 35 247 48 755.5 0 48 248 62 755.5 0 62 249 37 755.5 0 37 250 102 755.5 0 102 251 54 755.5 0 54 252 57 755.5 0 57 253 65 755.5 0 65 254 38 755.5 0 38 255 35 755.5 0 35 256 39 755.5 0 39 257 135 755.5 0 135 258 81 755.5 0 81 259 38 755.5 0 38 260 97 755.5 0 97 261 54 755.5 0 54 262 63 755.5 0 63 263 60 755.5 0 60 264 54 755.5 0 54 265 77 755.5 0 77 266 55 755.5 0 55 267 32 755.5 0 32 268 88 755.5 0 88 269 167 755.5 0 167 270 631 755.5 0 631 271 174 755.5 0 174 272 923 755.5 0 923 273 1535 755.5 0 1535 274 408 755.5 0 408 275 45 755.5 0 45 276 46 755.5 0 46 277 44 755.5 0 44 278 47 755.5 0 47 279 49 755.5 0 49 280 60 755.5 0 60 281 63 755.5 0 63 282 227 755.5 0 227 283 321 755.5 0 321 284 294 755.5 0 294 285 23 755.5 0 23 286 70 755.5 0 70 287 68 755.5 0 68 288 153 755.5 0 153 289 113 755.5 0 113 290 174 755.5 0 174 291 1014 755.5 0 1014 292 1977 755.5 0 1977 293 659 755.5 0 659 294 586 755.5 0 586 295 162 755.5 0 162 296 53 755.5 0 53 297 61 755.5 0 61 298 344 755.5 0 344 299 480 755.5 0 480 300 749 755.5 0 749 301 34 755.5 0 34 Finished in 2.21 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 53,861 Reads with adapters: 14,454 (26.8%) Reads that were too short: 37 (0.1%) Reads written (passing filters): 14,417 (26.8%) Total basepairs processed: 11,357,210 bp Total written (filtered): 3,973,967 bp (35.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14454 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 1 3 16 58 0.0 3 0 50 5 3 17 10898 0.0 3 10529 326 36 7 18 41 0.0 3 11 22 5 3 19 67 0.0 3 5 37 11 14 20 3257 0.0 3 3149 89 9 10 21 7 0.0 3 4 1 1 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 52 16 0.0 3 13 2 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 92 8 0.0 3 0 0 0 8 94 1 0.0 3 0 0 0 1 95 6 0.0 3 0 0 0 6 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 111 24 0.0 3 0 0 0 24 114 9 0.0 3 0 0 1 8 130 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 213 11 0.0 3 0 0 0 11 214 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 14,417 Reads with adapters: 1,639 (11.4%) Reads written (passing filters): 14,417 (100.0%) Total basepairs processed: 3,973,967 bp Total written (filtered): 3,969,017 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1639 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.3% C: 45.8% G: 16.4% T: 12.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1607 225.3 0 1607 4 31 56.3 0 31 5 1 14.1 0 1