This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 95.56 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 439,519 (14.2%) Reads that were too short: 111,476 (3.6%) Reads written (passing filters): 328,043 (10.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 54,075,114 bp (5.8%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 439519 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 30 755.5 0 30 7 15 755.5 0 15 8 22 755.5 0 22 9 19 755.5 0 19 10 18 755.5 0 18 11 19 755.5 0 19 12 13 755.5 0 13 13 20 755.5 0 20 14 11 755.5 0 11 15 17 755.5 0 17 16 15 755.5 0 15 17 20 755.5 0 20 18 10 755.5 0 10 19 18 755.5 0 18 20 17 755.5 0 17 21 13 755.5 0 13 22 15 755.5 0 15 23 14 755.5 0 14 24 16 755.5 0 16 25 23 755.5 0 23 26 14 755.5 0 14 27 21 755.5 0 21 28 20 755.5 0 20 29 9 755.5 0 9 30 17 755.5 0 17 31 19 755.5 0 19 32 19 755.5 0 19 33 17 755.5 0 17 34 17 755.5 0 17 35 11 755.5 0 11 36 17 755.5 0 17 37 15 755.5 0 15 38 12 755.5 0 12 39 16 755.5 0 16 40 20 755.5 0 20 41 16 755.5 0 16 42 18 755.5 0 18 43 16 755.5 0 16 44 22 755.5 0 22 45 29 755.5 0 29 46 23 755.5 0 23 47 26 755.5 0 26 48 26 755.5 0 26 49 21 755.5 0 21 50 13 755.5 0 13 51 35 755.5 0 35 52 23 755.5 0 23 53 13 755.5 0 13 54 14 755.5 0 14 55 20 755.5 0 20 56 64 755.5 0 64 57 233 755.5 0 233 58 25 755.5 0 25 59 26 755.5 0 26 60 18 755.5 0 18 61 14 755.5 0 14 62 21 755.5 0 21 63 15 755.5 0 15 64 10 755.5 0 10 65 19 755.5 0 19 66 26 755.5 0 26 67 32 755.5 0 32 68 35 755.5 0 35 69 24 755.5 0 24 70 42 755.5 0 42 71 30 755.5 0 30 72 198 755.5 0 198 73 195 755.5 0 195 74 635 755.5 0 635 75 1090 755.5 0 1090 76 1769 755.5 0 1769 77 558 755.5 0 558 78 1048 755.5 0 1048 79 279 755.5 0 279 80 332 755.5 0 332 81 479 755.5 0 479 82 447 755.5 0 447 83 328 755.5 0 328 84 165 755.5 0 165 85 423 755.5 0 423 86 163 755.5 0 163 87 574 755.5 0 574 88 634 755.5 0 634 89 273 755.5 0 273 90 851 755.5 0 851 91 1275 755.5 0 1275 92 128 755.5 0 128 93 906 755.5 0 906 94 1044 755.5 0 1044 95 979 755.5 0 979 96 687 755.5 0 687 97 382 755.5 0 382 98 280 755.5 0 280 99 171 755.5 0 171 100 157 755.5 0 157 101 351 755.5 0 351 102 396 755.5 0 396 103 247 755.5 0 247 104 685 755.5 0 685 105 649 755.5 0 649 106 367 755.5 0 367 107 4872 755.5 0 4872 108 771 755.5 0 771 109 442 755.5 0 442 110 337 755.5 0 337 111 637 755.5 0 637 112 463 755.5 0 463 113 563 755.5 0 563 114 583 755.5 0 583 115 273 755.5 0 273 116 571 755.5 0 571 117 332 755.5 0 332 118 373 755.5 0 373 119 833 755.5 0 833 120 1842 755.5 0 1842 121 1572 755.5 0 1572 122 1111 755.5 0 1111 123 1380 755.5 0 1380 124 1110 755.5 0 1110 125 924 755.5 0 924 126 1542 755.5 0 1542 127 1742 755.5 0 1742 128 1676 755.5 0 1676 129 1724 755.5 0 1724 130 1779 755.5 0 1779 131 2774 755.5 0 2774 132 2848 755.5 0 2848 133 30933 755.5 0 30933 134 37282 755.5 0 37282 135 72474 755.5 0 72474 136 75972 755.5 0 75972 137 948 755.5 0 948 138 504 755.5 0 504 139 632 755.5 0 632 140 1024 755.5 0 1024 141 742 755.5 0 742 142 764 755.5 0 764 143 344 755.5 0 344 144 466 755.5 0 466 145 1085 755.5 0 1085 146 1126 755.5 0 1126 147 1407 755.5 0 1407 148 816 755.5 0 816 149 728 755.5 0 728 150 607 755.5 0 607 151 484 755.5 0 484 152 1071 755.5 0 1071 153 682 755.5 0 682 154 1012 755.5 0 1012 155 1436 755.5 0 1436 156 1912 755.5 0 1912 157 2694 755.5 0 2694 158 1291 755.5 0 1291 159 2138 755.5 0 2138 160 1129 755.5 0 1129 161 807 755.5 0 807 162 784 755.5 0 784 163 570 755.5 0 570 164 620 755.5 0 620 165 886 755.5 0 886 166 1105 755.5 0 1105 167 762 755.5 0 762 168 453 755.5 0 453 169 554 755.5 0 554 170 375 755.5 0 375 171 608 755.5 0 608 172 511 755.5 0 511 173 1381 755.5 0 1381 174 1783 755.5 0 1783 175 1423 755.5 0 1423 176 599 755.5 0 599 177 285 755.5 0 285 178 306 755.5 0 306 179 375 755.5 0 375 180 890 755.5 0 890 181 1180 755.5 0 1180 182 941 755.5 0 941 183 581 755.5 0 581 184 553 755.5 0 553 185 1001 755.5 0 1001 186 1685 755.5 0 1685 187 944 755.5 0 944 188 689 755.5 0 689 189 388 755.5 0 388 190 932 755.5 0 932 191 1421 755.5 0 1421 192 1153 755.5 0 1153 193 1101 755.5 0 1101 194 572 755.5 0 572 195 469 755.5 0 469 196 370 755.5 0 370 197 309 755.5 0 309 198 760 755.5 0 760 199 924 755.5 0 924 200 644 755.5 0 644 201 889 755.5 0 889 202 577 755.5 0 577 203 777 755.5 0 777 204 832 755.5 0 832 205 827 755.5 0 827 206 686 755.5 0 686 207 861 755.5 0 861 208 687 755.5 0 687 209 393 755.5 0 393 210 916 755.5 0 916 211 732 755.5 0 732 212 669 755.5 0 669 213 736 755.5 0 736 214 676 755.5 0 676 215 736 755.5 0 736 216 535 755.5 0 535 217 583 755.5 0 583 218 977 755.5 0 977 219 1181 755.5 0 1181 220 1151 755.5 0 1151 221 957 755.5 0 957 222 421 755.5 0 421 223 324 755.5 0 324 224 1274 755.5 0 1274 225 951 755.5 0 951 226 817 755.5 0 817 227 988 755.5 0 988 228 981 755.5 0 981 229 835 755.5 0 835 230 751 755.5 0 751 231 729 755.5 0 729 232 1043 755.5 0 1043 233 765 755.5 0 765 234 608 755.5 0 608 235 870 755.5 0 870 236 816 755.5 0 816 237 636 755.5 0 636 238 541 755.5 0 541 239 695 755.5 0 695 240 831 755.5 0 831 241 757 755.5 0 757 242 663 755.5 0 663 243 498 755.5 0 498 244 672 755.5 0 672 245 1108 755.5 0 1108 246 1239 755.5 0 1239 247 966 755.5 0 966 248 685 755.5 0 685 249 385 755.5 0 385 250 393 755.5 0 393 251 556 755.5 0 556 252 1123 755.5 0 1123 253 804 755.5 0 804 254 919 755.5 0 919 255 1493 755.5 0 1493 256 1872 755.5 0 1872 257 1427 755.5 0 1427 258 1126 755.5 0 1126 259 772 755.5 0 772 260 759 755.5 0 759 261 568 755.5 0 568 262 454 755.5 0 454 263 497 755.5 0 497 264 453 755.5 0 453 265 824 755.5 0 824 266 1499 755.5 0 1499 267 1218 755.5 0 1218 268 2167 755.5 0 2167 269 1137 755.5 0 1137 270 939 755.5 0 939 271 830 755.5 0 830 272 1072 755.5 0 1072 273 884 755.5 0 884 274 1090 755.5 0 1090 275 3344 755.5 0 3344 276 6624 755.5 0 6624 277 5238 755.5 0 5238 278 1421 755.5 0 1421 279 1792 755.5 0 1792 280 2086 755.5 0 2086 281 1191 755.5 0 1191 282 759 755.5 0 759 283 569 755.5 0 569 284 953 755.5 0 953 285 999 755.5 0 999 286 1906 755.5 0 1906 287 1594 755.5 0 1594 288 1027 755.5 0 1027 289 697 755.5 0 697 290 477 755.5 0 477 291 582 755.5 0 582 292 1080 755.5 0 1080 293 1138 755.5 0 1138 294 1799 755.5 0 1799 295 3011 755.5 0 3011 296 5125 755.5 0 5125 297 2339 755.5 0 2339 298 921 755.5 0 921 299 1112 755.5 0 1112 300 827 755.5 0 827 301 651 755.5 0 651 Finished in 11.32 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 328,043 Reads with adapters: 511 (0.2%) Reads that were too short: 87 (0.0%) Reads written (passing filters): 424 (0.1%) Total basepairs processed: 54,075,114 bp Total written (filtered): 54,045 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 511 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 3 1 19 1 0.0 3 0 0 1 20 2 0.0 3 2 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 246 0.0 3 0 0 1 245 35 15 0.0 3 0 0 0 15 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 1 1 38 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 1 46 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 1 58 98 0.0 3 0 0 0 98 59 27 0.0 3 0 0 0 27 64 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 62 0.0 3 0 0 1 61 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (24 us/read; 2.54 M reads/minute). === Summary === Total reads processed: 424 Reads with adapters: 31 (7.3%) Reads written (passing filters): 424 (100.0%) Total basepairs processed: 54,045 bp Total written (filtered): 53,947 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 31 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.6% C: 0.0% G: 19.4% T: 29.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 6.6 0 26 4 5 1.7 0 5