This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.01 s (29 us/read; 2.04 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 109,071 (3.5%) Reads that were too short: 12,490 (0.4%) Reads written (passing filters): 96,581 (3.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 24,789,987 bp (2.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 109071 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1128 755.5 0 1128 7 356 755.5 0 356 8 768 755.5 0 768 9 38789 755.5 0 38789 10 60 755.5 0 60 11 8 755.5 0 8 12 13 755.5 0 13 13 18 755.5 0 18 14 16 755.5 0 16 15 17 755.5 0 17 16 30 755.5 0 30 17 24 755.5 0 24 18 18 755.5 0 18 19 13 755.5 0 13 20 18 755.5 0 18 21 16 755.5 0 16 22 16 755.5 0 16 23 24 755.5 0 24 24 17 755.5 0 17 25 23 755.5 0 23 26 26 755.5 0 26 27 39 755.5 0 39 28 54 755.5 0 54 29 115 755.5 0 115 30 36 755.5 0 36 31 52 755.5 0 52 32 133 755.5 0 133 33 1134 755.5 0 1134 34 175 755.5 0 175 35 51 755.5 0 51 36 71 755.5 0 71 37 90 755.5 0 90 38 97 755.5 0 97 39 80 755.5 0 80 40 82 755.5 0 82 41 127 755.5 0 127 42 1672 755.5 0 1672 43 3745 755.5 0 3745 44 6338 755.5 0 6338 45 41 755.5 0 41 46 18 755.5 0 18 47 33 755.5 0 33 48 127 755.5 0 127 49 38 755.5 0 38 50 29 755.5 0 29 51 36 755.5 0 36 52 37 755.5 0 37 53 76 755.5 0 76 54 65 755.5 0 65 55 51 755.5 0 51 56 31 755.5 0 31 57 49 755.5 0 49 58 100 755.5 0 100 59 76 755.5 0 76 60 128 755.5 0 128 61 187 755.5 0 187 62 224 755.5 0 224 63 383 755.5 0 383 64 957 755.5 0 957 65 13763 755.5 0 13763 66 5653 755.5 0 5653 67 10609 755.5 0 10609 68 55 755.5 0 55 69 23 755.5 0 23 70 14 755.5 0 14 71 42 755.5 0 42 72 17 755.5 0 17 73 15 755.5 0 15 74 13 755.5 0 13 75 11 755.5 0 11 76 8 755.5 0 8 77 9 755.5 0 9 78 15 755.5 0 15 79 13 755.5 0 13 80 18 755.5 0 18 81 34 755.5 0 34 82 50 755.5 0 50 83 20 755.5 0 20 84 23 755.5 0 23 85 55 755.5 0 55 86 23 755.5 0 23 87 90 755.5 0 90 88 24 755.5 0 24 89 15 755.5 0 15 90 35 755.5 0 35 91 41 755.5 0 41 92 25 755.5 0 25 93 35 755.5 0 35 94 22 755.5 0 22 95 26 755.5 0 26 96 92 755.5 0 92 97 596 755.5 0 596 98 39 755.5 0 39 99 18 755.5 0 18 100 30 755.5 0 30 101 54 755.5 0 54 102 151 755.5 0 151 103 137 755.5 0 137 104 75 755.5 0 75 105 23 755.5 0 23 106 30 755.5 0 30 107 41 755.5 0 41 108 54 755.5 0 54 109 16 755.5 0 16 110 17 755.5 0 17 111 27 755.5 0 27 112 67 755.5 0 67 113 41 755.5 0 41 114 22 755.5 0 22 115 33 755.5 0 33 116 18 755.5 0 18 117 27 755.5 0 27 118 35 755.5 0 35 119 71 755.5 0 71 120 64 755.5 0 64 121 73 755.5 0 73 122 68 755.5 0 68 123 67 755.5 0 67 124 54 755.5 0 54 125 50 755.5 0 50 126 46 755.5 0 46 127 73 755.5 0 73 128 98 755.5 0 98 129 42 755.5 0 42 130 29 755.5 0 29 131 34 755.5 0 34 132 58 755.5 0 58 133 156 755.5 0 156 134 154 755.5 0 154 135 76 755.5 0 76 136 95 755.5 0 95 137 101 755.5 0 101 138 240 755.5 0 240 139 160 755.5 0 160 140 102 755.5 0 102 141 131 755.5 0 131 142 93 755.5 0 93 143 54 755.5 0 54 144 36 755.5 0 36 145 33 755.5 0 33 146 55 755.5 0 55 147 73 755.5 0 73 148 52 755.5 0 52 149 62 755.5 0 62 150 69 755.5 0 69 151 34 755.5 0 34 152 24 755.5 0 24 153 39 755.5 0 39 154 33 755.5 0 33 155 54 755.5 0 54 156 47 755.5 0 47 157 39 755.5 0 39 158 54 755.5 0 54 159 158 755.5 0 158 160 83 755.5 0 83 161 83 755.5 0 83 162 65 755.5 0 65 163 52 755.5 0 52 164 57 755.5 0 57 165 55 755.5 0 55 166 73 755.5 0 73 167 71 755.5 0 71 168 82 755.5 0 82 169 39 755.5 0 39 170 92 755.5 0 92 171 108 755.5 0 108 172 88 755.5 0 88 173 43 755.5 0 43 174 75 755.5 0 75 175 60 755.5 0 60 176 64 755.5 0 64 177 49 755.5 0 49 178 29 755.5 0 29 179 35 755.5 0 35 180 40 755.5 0 40 181 70 755.5 0 70 182 91 755.5 0 91 183 66 755.5 0 66 184 99 755.5 0 99 185 92 755.5 0 92 186 141 755.5 0 141 187 105 755.5 0 105 188 75 755.5 0 75 189 45 755.5 0 45 190 35 755.5 0 35 191 37 755.5 0 37 192 63 755.5 0 63 193 46 755.5 0 46 194 72 755.5 0 72 195 92 755.5 0 92 196 42 755.5 0 42 197 31 755.5 0 31 198 28 755.5 0 28 199 63 755.5 0 63 200 177 755.5 0 177 201 103 755.5 0 103 202 33 755.5 0 33 203 37 755.5 0 37 204 41 755.5 0 41 205 63 755.5 0 63 206 164 755.5 0 164 207 246 755.5 0 246 208 142 755.5 0 142 209 75 755.5 0 75 210 55 755.5 0 55 211 79 755.5 0 79 212 82 755.5 0 82 213 51 755.5 0 51 214 103 755.5 0 103 215 139 755.5 0 139 216 146 755.5 0 146 217 253 755.5 0 253 218 123 755.5 0 123 219 144 755.5 0 144 220 194 755.5 0 194 221 113 755.5 0 113 222 55 755.5 0 55 223 79 755.5 0 79 224 158 755.5 0 158 225 158 755.5 0 158 226 110 755.5 0 110 227 117 755.5 0 117 228 100 755.5 0 100 229 98 755.5 0 98 230 112 755.5 0 112 231 191 755.5 0 191 232 97 755.5 0 97 233 49 755.5 0 49 234 30 755.5 0 30 235 59 755.5 0 59 236 127 755.5 0 127 237 152 755.5 0 152 238 108 755.5 0 108 239 114 755.5 0 114 240 128 755.5 0 128 241 88 755.5 0 88 242 54 755.5 0 54 243 63 755.5 0 63 244 125 755.5 0 125 245 141 755.5 0 141 246 124 755.5 0 124 247 127 755.5 0 127 248 111 755.5 0 111 249 177 755.5 0 177 250 59 755.5 0 59 251 34 755.5 0 34 252 24 755.5 0 24 253 55 755.5 0 55 254 132 755.5 0 132 255 778 755.5 0 778 256 540 755.5 0 540 257 430 755.5 0 430 258 283 755.5 0 283 259 207 755.5 0 207 260 118 755.5 0 118 261 56 755.5 0 56 262 47 755.5 0 47 263 53 755.5 0 53 264 104 755.5 0 104 265 133 755.5 0 133 266 139 755.5 0 139 267 162 755.5 0 162 268 193 755.5 0 193 269 171 755.5 0 171 270 93 755.5 0 93 271 73 755.5 0 73 272 46 755.5 0 46 273 39 755.5 0 39 274 262 755.5 0 262 275 246 755.5 0 246 276 186 755.5 0 186 277 63 755.5 0 63 278 58 755.5 0 58 279 64 755.5 0 64 280 135 755.5 0 135 281 150 755.5 0 150 282 91 755.5 0 91 283 52 755.5 0 52 284 46 755.5 0 46 285 78 755.5 0 78 286 82 755.5 0 82 287 105 755.5 0 105 288 163 755.5 0 163 289 145 755.5 0 145 290 92 755.5 0 92 291 42 755.5 0 42 292 25 755.5 0 25 293 174 755.5 0 174 294 261 755.5 0 261 295 189 755.5 0 189 296 106 755.5 0 106 297 77 755.5 0 77 298 59 755.5 0 59 299 48 755.5 0 48 300 57 755.5 0 57 301 130 755.5 0 130 Finished in 5.21 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 96,581 Reads with adapters: 1,321 (1.4%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 1,310 (1.4%) Total basepairs processed: 24,789,987 bp Total written (filtered): 317,918 bp (1.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1321 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 1 0.0 3 0 1 20 10 0.0 4 10 21 1 0.0 4 0 1 22 7 0.0 4 1 6 23 936 0.0 4 921 14 1 24 2 0.0 4 1 1 42 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 7 0.0 4 0 0 0 0 7 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 256 0.0 4 0 0 0 0 256 134 2 0.0 4 0 0 0 0 2 135 78 0.0 4 0 0 0 0 78 145 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 193 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 1,310 Reads with adapters: 17 (1.3%) Reads written (passing filters): 1,310 (100.0%) Total basepairs processed: 317,918 bp Total written (filtered): 317,371 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 17 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.9% C: 23.5% G: 70.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 20.5 0 2 9 2 0.0 0 1 1 10 9 0.0 1 0 9 11 2 0.0 1 0 2 150 1 0.0 1 0 1 261 1 0.0 1 1