This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.76 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 65,710 (2.1%) Reads that were too short: 36,383 (1.2%) Reads written (passing filters): 29,327 (0.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 5,817,055 bp (0.6%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 65710 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 755.5 0 129 7 199 755.5 0 199 8 10298 755.5 0 10298 9 34 755.5 0 34 10 11 755.5 0 11 11 2 755.5 0 2 12 1 755.5 0 1 13 5 755.5 0 5 14 7 755.5 0 7 15 25 755.5 0 25 16 5 755.5 0 5 17 8 755.5 0 8 18 12 755.5 0 12 19 58 755.5 0 58 20 28 755.5 0 28 21 4 755.5 0 4 22 2 755.5 0 2 23 4 755.5 0 4 24 3 755.5 0 3 25 8 755.5 0 8 26 10 755.5 0 10 27 16 755.5 0 16 28 4 755.5 0 4 29 4 755.5 0 4 30 3 755.5 0 3 32 1 755.5 0 1 33 5 755.5 0 5 34 8 755.5 0 8 35 5 755.5 0 5 36 5 755.5 0 5 37 3 755.5 0 3 38 2 755.5 0 2 39 1 755.5 0 1 40 5 755.5 0 5 41 2 755.5 0 2 42 4 755.5 0 4 43 15 755.5 0 15 44 63 755.5 0 63 45 56 755.5 0 56 46 14 755.5 0 14 47 5 755.5 0 5 48 6 755.5 0 6 49 3 755.5 0 3 50 7 755.5 0 7 51 1 755.5 0 1 52 5 755.5 0 5 53 5 755.5 0 5 54 6 755.5 0 6 55 8 755.5 0 8 56 19 755.5 0 19 57 6 755.5 0 6 58 5 755.5 0 5 59 6 755.5 0 6 60 12 755.5 0 12 61 6 755.5 0 6 62 7 755.5 0 7 63 2 755.5 0 2 64 6 755.5 0 6 65 14 755.5 0 14 66 6 755.5 0 6 67 10 755.5 0 10 68 16 755.5 0 16 69 13 755.5 0 13 70 19 755.5 0 19 71 32 755.5 0 32 72 8 755.5 0 8 73 19 755.5 0 19 74 36 755.5 0 36 75 43 755.5 0 43 76 118 755.5 0 118 77 698 755.5 0 698 78 631 755.5 0 631 79 209 755.5 0 209 80 52 755.5 0 52 81 64 755.5 0 64 82 88 755.5 0 88 83 106 755.5 0 106 84 74 755.5 0 74 85 5 755.5 0 5 86 11 755.5 0 11 87 13 755.5 0 13 88 18 755.5 0 18 89 5 755.5 0 5 90 26 755.5 0 26 91 70 755.5 0 70 92 95 755.5 0 95 93 41 755.5 0 41 94 23 755.5 0 23 95 20 755.5 0 20 96 21 755.5 0 21 97 30 755.5 0 30 98 26 755.5 0 26 99 9 755.5 0 9 100 21 755.5 0 21 102 6 755.5 0 6 103 16 755.5 0 16 104 12 755.5 0 12 105 6 755.5 0 6 106 5 755.5 0 5 107 11 755.5 0 11 108 20 755.5 0 20 109 12 755.5 0 12 110 6 755.5 0 6 111 4 755.5 0 4 112 10 755.5 0 10 113 23 755.5 0 23 114 53 755.5 0 53 115 41 755.5 0 41 116 85 755.5 0 85 117 109 755.5 0 109 118 16 755.5 0 16 119 8 755.5 0 8 120 16 755.5 0 16 121 24 755.5 0 24 122 37 755.5 0 37 123 80 755.5 0 80 124 53 755.5 0 53 125 26 755.5 0 26 126 14 755.5 0 14 127 30 755.5 0 30 128 30 755.5 0 30 129 54 755.5 0 54 130 25 755.5 0 25 131 11 755.5 0 11 132 13 755.5 0 13 133 20 755.5 0 20 134 48 755.5 0 48 135 141 755.5 0 141 136 114 755.5 0 114 137 65 755.5 0 65 138 185 755.5 0 185 139 23 755.5 0 23 140 23 755.5 0 23 141 74 755.5 0 74 142 41 755.5 0 41 143 105 755.5 0 105 144 21 755.5 0 21 145 22 755.5 0 22 146 19 755.5 0 19 147 16 755.5 0 16 148 28 755.5 0 28 149 21 755.5 0 21 150 86 755.5 0 86 151 29 755.5 0 29 152 18 755.5 0 18 153 10 755.5 0 10 154 24 755.5 0 24 155 40 755.5 0 40 156 31 755.5 0 31 157 17 755.5 0 17 158 36 755.5 0 36 159 21 755.5 0 21 160 26 755.5 0 26 161 17 755.5 0 17 162 41 755.5 0 41 163 85 755.5 0 85 164 88 755.5 0 88 165 75 755.5 0 75 166 53 755.5 0 53 167 42 755.5 0 42 168 47 755.5 0 47 169 66 755.5 0 66 170 21 755.5 0 21 171 110 755.5 0 110 172 64 755.5 0 64 173 86 755.5 0 86 174 232 755.5 0 232 175 6756 755.5 0 6756 176 169 755.5 0 169 177 56 755.5 0 56 178 75 755.5 0 75 179 54 755.5 0 54 180 271 755.5 0 271 181 111 755.5 0 111 182 169 755.5 0 169 183 45 755.5 0 45 184 57 755.5 0 57 185 38 755.5 0 38 186 36 755.5 0 36 187 53 755.5 0 53 188 62 755.5 0 62 189 174 755.5 0 174 190 210 755.5 0 210 191 1407 755.5 0 1407 192 450 755.5 0 450 193 1663 755.5 0 1663 194 41 755.5 0 41 195 30 755.5 0 30 196 61 755.5 0 61 197 32 755.5 0 32 198 25 755.5 0 25 199 39 755.5 0 39 200 63 755.5 0 63 201 119 755.5 0 119 202 721 755.5 0 721 203 123 755.5 0 123 204 72 755.5 0 72 205 35 755.5 0 35 206 11 755.5 0 11 207 24 755.5 0 24 208 76 755.5 0 76 209 90 755.5 0 90 210 67 755.5 0 67 211 93 755.5 0 93 212 32 755.5 0 32 213 19 755.5 0 19 214 16 755.5 0 16 215 25 755.5 0 25 216 27 755.5 0 27 217 25 755.5 0 25 218 15 755.5 0 15 219 16 755.5 0 16 220 40 755.5 0 40 221 34 755.5 0 34 222 15 755.5 0 15 223 7 755.5 0 7 224 12 755.5 0 12 225 26 755.5 0 26 226 73 755.5 0 73 227 38 755.5 0 38 228 26 755.5 0 26 229 52 755.5 0 52 230 9 755.5 0 9 231 20 755.5 0 20 232 18 755.5 0 18 233 27 755.5 0 27 234 37 755.5 0 37 235 23 755.5 0 23 236 23 755.5 0 23 237 45 755.5 0 45 238 18 755.5 0 18 239 11 755.5 0 11 240 20 755.5 0 20 241 18 755.5 0 18 242 7 755.5 0 7 243 10 755.5 0 10 244 11 755.5 0 11 245 10 755.5 0 10 246 19 755.5 0 19 247 35 755.5 0 35 248 33 755.5 0 33 249 50 755.5 0 50 250 67 755.5 0 67 251 56 755.5 0 56 252 46 755.5 0 46 253 42 755.5 0 42 254 234 755.5 0 234 255 64 755.5 0 64 256 43 755.5 0 43 257 45 755.5 0 45 258 73 755.5 0 73 259 372 755.5 0 372 260 597 755.5 0 597 261 562 755.5 0 562 262 762 755.5 0 762 263 388 755.5 0 388 264 108 755.5 0 108 265 110 755.5 0 110 266 110 755.5 0 110 267 25 755.5 0 25 268 37 755.5 0 37 269 102 755.5 0 102 270 101 755.5 0 101 271 181 755.5 0 181 272 54 755.5 0 54 273 45 755.5 0 45 274 88 755.5 0 88 275 108 755.5 0 108 276 178 755.5 0 178 277 379 755.5 0 379 278 106 755.5 0 106 279 64 755.5 0 64 280 110 755.5 0 110 281 338 755.5 0 338 282 267 755.5 0 267 283 292 755.5 0 292 284 785 755.5 0 785 285 1538 755.5 0 1538 286 21528 755.5 0 21528 287 210 755.5 0 210 288 367 755.5 0 367 289 1587 755.5 0 1587 290 421 755.5 0 421 291 123 755.5 0 123 292 8 755.5 0 8 293 10 755.5 0 10 294 23 755.5 0 23 295 141 755.5 0 141 296 215 755.5 0 215 297 884 755.5 0 884 298 23 755.5 0 23 299 6 755.5 0 6 300 14 755.5 0 14 301 92 755.5 0 92 Finished in 1.18 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 29,327 Reads with adapters: 10,640 (36.3%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 10,630 (36.2%) Total basepairs processed: 5,817,055 bp Total written (filtered): 2,922,834 bp (50.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10640 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 1 1 16 89 0.0 3 0 75 6 8 17 10316 0.0 3 10041 251 18 6 18 91 0.0 3 3 38 13 37 19 85 0.0 3 5 0 55 25 20 10 0.0 3 1 0 0 9 21 11 0.0 3 0 0 1 10 23 1 0.0 3 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 81 16 0.0 3 14 1 1 83 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 104 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 10,630 Reads with adapters: 157 (1.5%) Reads written (passing filters): 10,630 (100.0%) Total basepairs processed: 2,922,834 bp Total written (filtered): 2,922,295 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 157 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.9% C: 24.2% G: 9.6% T: 22.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 111 166.1 0 111 4 24 41.5 0 24 5 22 10.4 0 22