This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.86 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 89,966 (2.9%) Reads that were too short: 16,064 (0.5%) Reads written (passing filters): 73,902 (2.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 20,681,224 bp (2.2%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 89966 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 577 755.5 0 577 7 910 755.5 0 910 8 51804 755.5 0 51804 9 10175 755.5 0 10175 10 19 755.5 0 19 11 7 755.5 0 7 12 7 755.5 0 7 13 5 755.5 0 5 14 4 755.5 0 4 15 7 755.5 0 7 16 6 755.5 0 6 17 9 755.5 0 9 18 7 755.5 0 7 19 6 755.5 0 6 20 4 755.5 0 4 21 5 755.5 0 5 22 12 755.5 0 12 23 4 755.5 0 4 24 26 755.5 0 26 25 8 755.5 0 8 26 11 755.5 0 11 27 3 755.5 0 3 28 7 755.5 0 7 29 7 755.5 0 7 30 10 755.5 0 10 31 1 755.5 0 1 32 5 755.5 0 5 33 3 755.5 0 3 34 10 755.5 0 10 35 9 755.5 0 9 36 9 755.5 0 9 37 4 755.5 0 4 38 7 755.5 0 7 39 5 755.5 0 5 40 5 755.5 0 5 41 3 755.5 0 3 42 5 755.5 0 5 43 5 755.5 0 5 44 11 755.5 0 11 45 4 755.5 0 4 46 8 755.5 0 8 47 17 755.5 0 17 48 17 755.5 0 17 49 2041 755.5 0 2041 50 26 755.5 0 26 51 13 755.5 0 13 52 6 755.5 0 6 53 5 755.5 0 5 54 6 755.5 0 6 55 6 755.5 0 6 56 5 755.5 0 5 57 8 755.5 0 8 58 8 755.5 0 8 59 4 755.5 0 4 60 8 755.5 0 8 61 5 755.5 0 5 62 4 755.5 0 4 63 13 755.5 0 13 64 5 755.5 0 5 65 13 755.5 0 13 66 9 755.5 0 9 67 25 755.5 0 25 68 66 755.5 0 66 69 1069 755.5 0 1069 70 25 755.5 0 25 71 81 755.5 0 81 72 7 755.5 0 7 73 4 755.5 0 4 74 9 755.5 0 9 75 9 755.5 0 9 76 38 755.5 0 38 77 14 755.5 0 14 78 35 755.5 0 35 79 22 755.5 0 22 80 355 755.5 0 355 81 59 755.5 0 59 82 133 755.5 0 133 83 19 755.5 0 19 84 11 755.5 0 11 85 9 755.5 0 9 86 12 755.5 0 12 87 5 755.5 0 5 88 11 755.5 0 11 89 10 755.5 0 10 90 19 755.5 0 19 91 24 755.5 0 24 92 24 755.5 0 24 93 23 755.5 0 23 94 21 755.5 0 21 95 37 755.5 0 37 96 254 755.5 0 254 97 428 755.5 0 428 98 1728 755.5 0 1728 99 45 755.5 0 45 100 27 755.5 0 27 101 7 755.5 0 7 102 12 755.5 0 12 103 7 755.5 0 7 104 9 755.5 0 9 105 7 755.5 0 7 106 6 755.5 0 6 107 8 755.5 0 8 108 9 755.5 0 9 109 33 755.5 0 33 110 15 755.5 0 15 111 11 755.5 0 11 112 196 755.5 0 196 113 22 755.5 0 22 114 62 755.5 0 62 115 16 755.5 0 16 116 10 755.5 0 10 117 11 755.5 0 11 118 12 755.5 0 12 119 8 755.5 0 8 120 35 755.5 0 35 121 14 755.5 0 14 122 19 755.5 0 19 123 6 755.5 0 6 124 13 755.5 0 13 125 6 755.5 0 6 126 9 755.5 0 9 127 11 755.5 0 11 128 35 755.5 0 35 129 18 755.5 0 18 130 37 755.5 0 37 131 23 755.5 0 23 132 162 755.5 0 162 133 34 755.5 0 34 134 112 755.5 0 112 135 33 755.5 0 33 136 18 755.5 0 18 137 18 755.5 0 18 138 12 755.5 0 12 139 74 755.5 0 74 140 13 755.5 0 13 141 22 755.5 0 22 142 15 755.5 0 15 143 17 755.5 0 17 144 9 755.5 0 9 145 13 755.5 0 13 146 16 755.5 0 16 147 11 755.5 0 11 148 18 755.5 0 18 149 24 755.5 0 24 150 60 755.5 0 60 151 12 755.5 0 12 152 10 755.5 0 10 153 11 755.5 0 11 154 14 755.5 0 14 155 16 755.5 0 16 156 16 755.5 0 16 157 15 755.5 0 15 158 22 755.5 0 22 159 18 755.5 0 18 160 13 755.5 0 13 161 16 755.5 0 16 162 16 755.5 0 16 163 12 755.5 0 12 164 25 755.5 0 25 165 9 755.5 0 9 166 19 755.5 0 19 167 17 755.5 0 17 168 38 755.5 0 38 169 12 755.5 0 12 170 16 755.5 0 16 171 14 755.5 0 14 172 21 755.5 0 21 173 14 755.5 0 14 174 15 755.5 0 15 175 14 755.5 0 14 176 21 755.5 0 21 177 11 755.5 0 11 178 21 755.5 0 21 179 16 755.5 0 16 180 12 755.5 0 12 181 27 755.5 0 27 182 32 755.5 0 32 183 67 755.5 0 67 184 60 755.5 0 60 185 87 755.5 0 87 186 171 755.5 0 171 187 27 755.5 0 27 188 196 755.5 0 196 189 240 755.5 0 240 190 294 755.5 0 294 191 47 755.5 0 47 192 47 755.5 0 47 193 13 755.5 0 13 194 10 755.5 0 10 195 14 755.5 0 14 196 34 755.5 0 34 197 21 755.5 0 21 198 10 755.5 0 10 199 9 755.5 0 9 200 8 755.5 0 8 201 23 755.5 0 23 202 25 755.5 0 25 203 12 755.5 0 12 204 14 755.5 0 14 205 20 755.5 0 20 206 27 755.5 0 27 207 30 755.5 0 30 208 42 755.5 0 42 209 175 755.5 0 175 210 179 755.5 0 179 211 397 755.5 0 397 212 73 755.5 0 73 213 29 755.5 0 29 214 25 755.5 0 25 215 43 755.5 0 43 216 22 755.5 0 22 217 32 755.5 0 32 218 16 755.5 0 16 219 15 755.5 0 15 220 11 755.5 0 11 221 30 755.5 0 30 222 54 755.5 0 54 223 53 755.5 0 53 224 37 755.5 0 37 225 79 755.5 0 79 226 56 755.5 0 56 227 45 755.5 0 45 228 126 755.5 0 126 229 36 755.5 0 36 230 22 755.5 0 22 231 25 755.5 0 25 232 68 755.5 0 68 233 77 755.5 0 77 234 69 755.5 0 69 235 71 755.5 0 71 236 120 755.5 0 120 237 52 755.5 0 52 238 47 755.5 0 47 239 60 755.5 0 60 240 190 755.5 0 190 241 623 755.5 0 623 242 4243 755.5 0 4243 243 840 755.5 0 840 244 42 755.5 0 42 245 51 755.5 0 51 246 384 755.5 0 384 247 85 755.5 0 85 248 610 755.5 0 610 249 191 755.5 0 191 250 449 755.5 0 449 251 3098 755.5 0 3098 252 34 755.5 0 34 253 75 755.5 0 75 254 172 755.5 0 172 255 436 755.5 0 436 256 55 755.5 0 55 257 21 755.5 0 21 258 31 755.5 0 31 259 25 755.5 0 25 260 50 755.5 0 50 261 94 755.5 0 94 262 121 755.5 0 121 263 29 755.5 0 29 264 33 755.5 0 33 265 50 755.5 0 50 266 32 755.5 0 32 267 19 755.5 0 19 268 41 755.5 0 41 269 23 755.5 0 23 270 163 755.5 0 163 271 507 755.5 0 507 272 92 755.5 0 92 273 34 755.5 0 34 274 49 755.5 0 49 275 41 755.5 0 41 276 33 755.5 0 33 277 24 755.5 0 24 278 27 755.5 0 27 279 12 755.5 0 12 280 23 755.5 0 23 281 38 755.5 0 38 282 41 755.5 0 41 283 27 755.5 0 27 284 25 755.5 0 25 285 37 755.5 0 37 286 16 755.5 0 16 287 8 755.5 0 8 288 19 755.5 0 19 289 38 755.5 0 38 290 59 755.5 0 59 291 23 755.5 0 23 292 10 755.5 0 10 293 9 755.5 0 9 294 16 755.5 0 16 295 17 755.5 0 17 296 50 755.5 0 50 297 26 755.5 0 26 298 30 755.5 0 30 299 38 755.5 0 38 300 28 755.5 0 28 301 43 755.5 0 43 Finished in 4.28 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 73,902 Reads with adapters: 11,203 (15.2%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 11,200 (15.2%) Total basepairs processed: 20,681,224 bp Total written (filtered): 2,971,257 bp (14.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 3 0.0 3 0 0 3 19 144 0.0 3 0 140 3 0 1 20 10322 0.0 4 10181 136 4 1 21 12 0.0 4 1 11 22 2 0.0 4 0 0 1 0 1 23 8 0.0 4 1 0 0 5 2 24 1 0.0 4 1 25 1 0.0 4 1 58 6 0.0 4 0 0 0 0 6 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 577 0.0 4 0 0 0 0 577 130 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 100 0.0 4 0 0 0 1 99 153 10 0.0 4 0 0 0 0 10 182 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 11,200 Reads with adapters: 522 (4.7%) Reads written (passing filters): 11,200 (100.0%) Total basepairs processed: 2,971,257 bp Total written (filtered): 2,907,751 bp (97.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 522 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 19.3% G: 26.6% T: 52.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 96 175.0 0 96 4 7 43.8 0 7 5 1 10.9 0 1 6 16 2.7 0 16 9 8 0.0 0 1 7 10 73 0.0 1 2 71 11 44 0.0 1 0 44 12 1 0.0 1 0 1 222 1 0.0 1 1 223 255 0.0 1 224 31 224 11 0.0 1 10 1 225 2 0.0 1 2 226 1 0.0 1 1 254 2 0.0 1 2 258 1 0.0 1 1 263 1 0.0 1 1 267 1 0.0 1 1 268 1 0.0 1 1