This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.38 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 45,167 (1.5%) Reads that were too short: 26,065 (0.8%) Reads written (passing filters): 19,102 (0.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 3,578,166 bp (0.4%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 45167 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 26 755.5 0 26 7 2891 755.5 0 2891 8 18 755.5 0 18 9 2 755.5 0 2 10 5 755.5 0 5 11 3 755.5 0 3 12 6 755.5 0 6 13 3 755.5 0 3 14 9 755.5 0 9 15 3 755.5 0 3 16 5 755.5 0 5 17 8 755.5 0 8 18 57 755.5 0 57 19 35 755.5 0 35 20 8 755.5 0 8 21 5 755.5 0 5 22 5 755.5 0 5 23 5 755.5 0 5 24 4 755.5 0 4 25 3 755.5 0 3 26 7 755.5 0 7 27 10 755.5 0 10 28 17 755.5 0 17 29 7 755.5 0 7 30 4 755.5 0 4 31 11 755.5 0 11 32 6 755.5 0 6 33 13 755.5 0 13 34 15 755.5 0 15 35 9 755.5 0 9 36 59 755.5 0 59 37 61 755.5 0 61 38 194 755.5 0 194 39 17 755.5 0 17 40 11 755.5 0 11 41 7 755.5 0 7 42 16 755.5 0 16 43 439 755.5 0 439 44 102 755.5 0 102 45 406 755.5 0 406 46 21 755.5 0 21 47 5 755.5 0 5 48 4 755.5 0 4 49 5 755.5 0 5 50 5 755.5 0 5 51 4 755.5 0 4 52 6 755.5 0 6 53 5 755.5 0 5 54 6 755.5 0 6 55 8 755.5 0 8 56 2 755.5 0 2 57 24 755.5 0 24 58 4 755.5 0 4 59 1 755.5 0 1 60 3 755.5 0 3 61 5 755.5 0 5 62 9 755.5 0 9 63 6 755.5 0 6 64 2 755.5 0 2 65 7 755.5 0 7 66 2 755.5 0 2 67 3 755.5 0 3 68 8 755.5 0 8 69 2 755.5 0 2 70 5 755.5 0 5 71 6 755.5 0 6 72 7 755.5 0 7 73 14 755.5 0 14 74 15 755.5 0 15 75 86 755.5 0 86 76 57 755.5 0 57 77 43 755.5 0 43 78 59 755.5 0 59 79 140 755.5 0 140 80 87 755.5 0 87 81 56 755.5 0 56 82 134 755.5 0 134 83 160 755.5 0 160 84 50 755.5 0 50 85 24 755.5 0 24 86 18 755.5 0 18 87 36 755.5 0 36 88 50 755.5 0 50 89 64 755.5 0 64 90 35 755.5 0 35 91 242 755.5 0 242 92 75 755.5 0 75 93 88 755.5 0 88 94 244 755.5 0 244 95 75 755.5 0 75 96 54 755.5 0 54 97 101 755.5 0 101 98 63 755.5 0 63 99 84 755.5 0 84 100 28 755.5 0 28 101 35 755.5 0 35 102 7 755.5 0 7 103 12 755.5 0 12 104 26 755.5 0 26 105 16 755.5 0 16 106 14 755.5 0 14 107 28 755.5 0 28 108 54 755.5 0 54 109 51 755.5 0 51 110 35 755.5 0 35 111 41 755.5 0 41 112 44 755.5 0 44 113 64 755.5 0 64 114 49 755.5 0 49 115 60 755.5 0 60 116 66 755.5 0 66 117 97 755.5 0 97 118 50 755.5 0 50 119 41 755.5 0 41 120 148 755.5 0 148 121 132 755.5 0 132 122 114 755.5 0 114 123 106 755.5 0 106 124 47 755.5 0 47 125 96 755.5 0 96 126 84 755.5 0 84 127 95 755.5 0 95 128 236 755.5 0 236 129 218 755.5 0 218 130 193 755.5 0 193 131 97 755.5 0 97 132 81 755.5 0 81 133 98 755.5 0 98 134 201 755.5 0 201 135 1105 755.5 0 1105 136 455 755.5 0 455 137 527 755.5 0 527 138 107 755.5 0 107 139 97 755.5 0 97 140 146 755.5 0 146 141 104 755.5 0 104 142 143 755.5 0 143 143 182 755.5 0 182 144 207 755.5 0 207 145 121 755.5 0 121 146 154 755.5 0 154 147 92 755.5 0 92 148 80 755.5 0 80 149 70 755.5 0 70 150 122 755.5 0 122 151 69 755.5 0 69 152 40 755.5 0 40 153 55 755.5 0 55 154 97 755.5 0 97 155 124 755.5 0 124 156 165 755.5 0 165 157 86 755.5 0 86 158 95 755.5 0 95 159 96 755.5 0 96 160 92 755.5 0 92 161 100 755.5 0 100 162 75 755.5 0 75 163 103 755.5 0 103 164 93 755.5 0 93 165 148 755.5 0 148 166 157 755.5 0 157 167 179 755.5 0 179 168 156 755.5 0 156 169 64 755.5 0 64 170 46 755.5 0 46 171 52 755.5 0 52 172 84 755.5 0 84 173 72 755.5 0 72 174 114 755.5 0 114 175 53 755.5 0 53 176 60 755.5 0 60 177 41 755.5 0 41 178 79 755.5 0 79 179 127 755.5 0 127 180 171 755.5 0 171 181 124 755.5 0 124 182 134 755.5 0 134 183 128 755.5 0 128 184 137 755.5 0 137 185 106 755.5 0 106 186 105 755.5 0 105 187 44 755.5 0 44 188 51 755.5 0 51 189 128 755.5 0 128 190 212 755.5 0 212 191 161 755.5 0 161 192 158 755.5 0 158 193 120 755.5 0 120 194 75 755.5 0 75 195 108 755.5 0 108 196 483 755.5 0 483 197 225 755.5 0 225 198 96 755.5 0 96 199 49 755.5 0 49 200 57 755.5 0 57 201 201 755.5 0 201 202 585 755.5 0 585 203 593 755.5 0 593 204 434 755.5 0 434 205 120 755.5 0 120 206 73 755.5 0 73 207 283 755.5 0 283 208 394 755.5 0 394 209 1069 755.5 0 1069 210 306 755.5 0 306 211 336 755.5 0 336 212 282 755.5 0 282 213 275 755.5 0 275 214 194 755.5 0 194 215 306 755.5 0 306 216 252 755.5 0 252 217 167 755.5 0 167 218 127 755.5 0 127 219 92 755.5 0 92 220 117 755.5 0 117 221 202 755.5 0 202 222 351 755.5 0 351 223 154 755.5 0 154 224 211 755.5 0 211 225 189 755.5 0 189 226 125 755.5 0 125 227 94 755.5 0 94 228 130 755.5 0 130 229 92 755.5 0 92 230 62 755.5 0 62 231 60 755.5 0 60 232 118 755.5 0 118 233 137 755.5 0 137 234 157 755.5 0 157 235 276 755.5 0 276 236 190 755.5 0 190 237 71 755.5 0 71 238 79 755.5 0 79 239 40 755.5 0 40 240 77 755.5 0 77 241 151 755.5 0 151 242 193 755.5 0 193 243 310 755.5 0 310 244 235 755.5 0 235 245 122 755.5 0 122 246 87 755.5 0 87 247 69 755.5 0 69 248 34 755.5 0 34 249 68 755.5 0 68 250 92 755.5 0 92 251 224 755.5 0 224 252 171 755.5 0 171 253 114 755.5 0 114 254 96 755.5 0 96 255 110 755.5 0 110 256 111 755.5 0 111 257 74 755.5 0 74 258 67 755.5 0 67 259 61 755.5 0 61 260 62 755.5 0 62 261 69 755.5 0 69 262 107 755.5 0 107 263 140 755.5 0 140 264 191 755.5 0 191 265 251 755.5 0 251 266 369 755.5 0 369 267 110 755.5 0 110 268 59 755.5 0 59 269 361 755.5 0 361 270 221 755.5 0 221 271 291 755.5 0 291 272 190 755.5 0 190 273 177 755.5 0 177 274 108 755.5 0 108 275 187 755.5 0 187 276 174 755.5 0 174 277 200 755.5 0 200 278 257 755.5 0 257 279 1784 755.5 0 1784 280 494 755.5 0 494 281 412 755.5 0 412 282 240 755.5 0 240 283 133 755.5 0 133 284 155 755.5 0 155 285 87 755.5 0 87 286 169 755.5 0 169 287 400 755.5 0 400 288 208 755.5 0 208 289 141 755.5 0 141 290 194 755.5 0 194 291 200 755.5 0 200 292 288 755.5 0 288 293 136 755.5 0 136 294 599 755.5 0 599 295 587 755.5 0 587 296 2117 755.5 0 2117 297 733 755.5 0 733 298 1091 755.5 0 1091 299 664 755.5 0 664 300 435 755.5 0 435 301 65 755.5 0 65 Finished in 0.77 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 19,102 Reads with adapters: 2,921 (15.3%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 2,913 (15.2%) Total basepairs processed: 3,578,166 bp Total written (filtered): 804,068 bp (22.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2921 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 0 2 16 25 0.0 3 0 21 2 2 17 2840 0.0 3 2755 70 13 2 18 8 0.0 3 0 4 1 3 19 14 0.0 3 5 0 5 4 20 4 0.0 3 1 1 0 2 26 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 1 116 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 129 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,913 Reads with adapters: 235 (8.1%) Reads written (passing filters): 2,913 (100.0%) Total basepairs processed: 804,068 bp Total written (filtered): 803,343 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 235 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.8% C: 42.1% G: 23.0% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 215 45.5 0 215 4 20 11.4 0 20