This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.04 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 38,758 (1.3%) Reads that were too short: 13,065 (0.4%) Reads written (passing filters): 25,693 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 4,292,378 bp (0.5%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 38758 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 152 755.5 0 152 7 107 755.5 0 107 8 90 755.5 0 90 9 5581 755.5 0 5581 10 14 755.5 0 14 11 17 755.5 0 17 12 18 755.5 0 18 13 29 755.5 0 29 14 24 755.5 0 24 15 18 755.5 0 18 16 23 755.5 0 23 17 42 755.5 0 42 18 28 755.5 0 28 19 34 755.5 0 34 20 16 755.5 0 16 21 33 755.5 0 33 22 18 755.5 0 18 23 24 755.5 0 24 24 15 755.5 0 15 25 10 755.5 0 10 26 26 755.5 0 26 27 16 755.5 0 16 28 22 755.5 0 22 29 18 755.5 0 18 30 20 755.5 0 20 31 20 755.5 0 20 32 27 755.5 0 27 33 25 755.5 0 25 34 18 755.5 0 18 35 17 755.5 0 17 36 14 755.5 0 14 37 10 755.5 0 10 38 24 755.5 0 24 39 14 755.5 0 14 40 16 755.5 0 16 41 37 755.5 0 37 42 37 755.5 0 37 43 40 755.5 0 40 44 311 755.5 0 311 45 19 755.5 0 19 46 23 755.5 0 23 47 20 755.5 0 20 48 15 755.5 0 15 49 8 755.5 0 8 50 102 755.5 0 102 51 27 755.5 0 27 52 32 755.5 0 32 53 20 755.5 0 20 54 17 755.5 0 17 55 14 755.5 0 14 56 19 755.5 0 19 57 16 755.5 0 16 58 18 755.5 0 18 59 19 755.5 0 19 60 13 755.5 0 13 61 13 755.5 0 13 62 17 755.5 0 17 63 9 755.5 0 9 64 12 755.5 0 12 65 16 755.5 0 16 66 17 755.5 0 17 67 17 755.5 0 17 68 6 755.5 0 6 69 20 755.5 0 20 70 6 755.5 0 6 71 12 755.5 0 12 72 11 755.5 0 11 73 39 755.5 0 39 74 34 755.5 0 34 75 73 755.5 0 73 76 31 755.5 0 31 77 80 755.5 0 80 78 20 755.5 0 20 79 18 755.5 0 18 80 23 755.5 0 23 81 61 755.5 0 61 82 19 755.5 0 19 83 18 755.5 0 18 84 30 755.5 0 30 85 14 755.5 0 14 86 75 755.5 0 75 87 22 755.5 0 22 88 16 755.5 0 16 89 20 755.5 0 20 90 21 755.5 0 21 91 19 755.5 0 19 92 157 755.5 0 157 93 29 755.5 0 29 94 65 755.5 0 65 95 18 755.5 0 18 96 58 755.5 0 58 97 18 755.5 0 18 98 12 755.5 0 12 99 6 755.5 0 6 100 12 755.5 0 12 101 8 755.5 0 8 102 8 755.5 0 8 103 10 755.5 0 10 104 8 755.5 0 8 105 5 755.5 0 5 106 14 755.5 0 14 107 11 755.5 0 11 108 20 755.5 0 20 109 9 755.5 0 9 110 22 755.5 0 22 111 16 755.5 0 16 112 14 755.5 0 14 113 35 755.5 0 35 114 66 755.5 0 66 115 18 755.5 0 18 116 12 755.5 0 12 117 13 755.5 0 13 118 15 755.5 0 15 119 34 755.5 0 34 120 21 755.5 0 21 121 25 755.5 0 25 122 31 755.5 0 31 123 169 755.5 0 169 124 60 755.5 0 60 125 168 755.5 0 168 126 20 755.5 0 20 127 29 755.5 0 29 128 26 755.5 0 26 129 23 755.5 0 23 130 10 755.5 0 10 131 43 755.5 0 43 132 117 755.5 0 117 133 19 755.5 0 19 134 18 755.5 0 18 135 17 755.5 0 17 136 28 755.5 0 28 137 25 755.5 0 25 138 22 755.5 0 22 139 13 755.5 0 13 140 20 755.5 0 20 141 22 755.5 0 22 142 13 755.5 0 13 143 8 755.5 0 8 144 24 755.5 0 24 145 10 755.5 0 10 146 29 755.5 0 29 147 22 755.5 0 22 148 48 755.5 0 48 149 11 755.5 0 11 150 9 755.5 0 9 151 12 755.5 0 12 152 9 755.5 0 9 153 3 755.5 0 3 154 9 755.5 0 9 155 18 755.5 0 18 156 15 755.5 0 15 157 12 755.5 0 12 158 48 755.5 0 48 159 19 755.5 0 19 160 26 755.5 0 26 161 24 755.5 0 24 162 24 755.5 0 24 163 29 755.5 0 29 164 22 755.5 0 22 165 37 755.5 0 37 166 21 755.5 0 21 167 47 755.5 0 47 168 35 755.5 0 35 169 26 755.5 0 26 170 88 755.5 0 88 171 20 755.5 0 20 172 74 755.5 0 74 173 63 755.5 0 63 174 890 755.5 0 890 175 586 755.5 0 586 176 89 755.5 0 89 177 36 755.5 0 36 178 36 755.5 0 36 179 49 755.5 0 49 180 188 755.5 0 188 181 215 755.5 0 215 182 39 755.5 0 39 183 26 755.5 0 26 184 38 755.5 0 38 185 26 755.5 0 26 186 53 755.5 0 53 187 49 755.5 0 49 188 58 755.5 0 58 189 12 755.5 0 12 190 9 755.5 0 9 191 23 755.5 0 23 192 85 755.5 0 85 193 74 755.5 0 74 194 298 755.5 0 298 195 148 755.5 0 148 196 29 755.5 0 29 197 19 755.5 0 19 198 175 755.5 0 175 199 1972 755.5 0 1972 200 9617 755.5 0 9617 201 184 755.5 0 184 202 28 755.5 0 28 203 13 755.5 0 13 204 15 755.5 0 15 205 31 755.5 0 31 206 55 755.5 0 55 207 33 755.5 0 33 208 17 755.5 0 17 209 13 755.5 0 13 210 25 755.5 0 25 211 26 755.5 0 26 212 19 755.5 0 19 213 18 755.5 0 18 214 44 755.5 0 44 215 19 755.5 0 19 216 20 755.5 0 20 217 31 755.5 0 31 218 33 755.5 0 33 219 128 755.5 0 128 220 234 755.5 0 234 221 160 755.5 0 160 222 64 755.5 0 64 223 42 755.5 0 42 224 31 755.5 0 31 225 39 755.5 0 39 226 35 755.5 0 35 227 55 755.5 0 55 228 73 755.5 0 73 229 22 755.5 0 22 230 33 755.5 0 33 231 30 755.5 0 30 232 15 755.5 0 15 233 15 755.5 0 15 234 11 755.5 0 11 235 33 755.5 0 33 236 58 755.5 0 58 237 76 755.5 0 76 238 312 755.5 0 312 239 466 755.5 0 466 240 7643 755.5 0 7643 241 197 755.5 0 197 242 93 755.5 0 93 243 10 755.5 0 10 244 18 755.5 0 18 245 26 755.5 0 26 246 43 755.5 0 43 247 76 755.5 0 76 248 109 755.5 0 109 249 45 755.5 0 45 250 52 755.5 0 52 251 13 755.5 0 13 252 23 755.5 0 23 253 11 755.5 0 11 254 34 755.5 0 34 255 44 755.5 0 44 256 119 755.5 0 119 257 209 755.5 0 209 258 608 755.5 0 608 259 16 755.5 0 16 260 8 755.5 0 8 261 8 755.5 0 8 262 3 755.5 0 3 263 17 755.5 0 17 264 10 755.5 0 10 265 13 755.5 0 13 266 19 755.5 0 19 267 52 755.5 0 52 268 41 755.5 0 41 269 23 755.5 0 23 270 15 755.5 0 15 271 9 755.5 0 9 272 6 755.5 0 6 273 7 755.5 0 7 274 13 755.5 0 13 275 14 755.5 0 14 276 11 755.5 0 11 277 12 755.5 0 12 278 10 755.5 0 10 279 32 755.5 0 32 280 25 755.5 0 25 281 31 755.5 0 31 282 9 755.5 0 9 283 9 755.5 0 9 284 14 755.5 0 14 285 43 755.5 0 43 286 259 755.5 0 259 287 62 755.5 0 62 288 61 755.5 0 61 289 66 755.5 0 66 290 68 755.5 0 68 291 123 755.5 0 123 292 37 755.5 0 37 293 40 755.5 0 40 294 22 755.5 0 22 295 21 755.5 0 21 296 8 755.5 0 8 297 23 755.5 0 23 298 42 755.5 0 42 299 8 755.5 0 8 300 7 755.5 0 7 301 3 755.5 0 3 Finished in 0.94 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 25,693 Reads with adapters: 5,825 (22.7%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 5,816 (22.6%) Total basepairs processed: 4,292,378 bp Total written (filtered): 1,595,963 bp (37.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5825 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 53 0.0 3 0 44 3 6 17 5608 0.0 3 5426 150 27 5 18 29 0.0 3 1 12 12 4 19 15 0.0 3 0 0 4 11 20 85 0.0 3 79 2 0 4 35 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 149 5 0.0 3 0 0 0 5 159 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 5,816 Reads with adapters: 735 (12.6%) Reads written (passing filters): 5,816 (100.0%) Total basepairs processed: 1,595,963 bp Total written (filtered): 1,593,690 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 735 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.9% C: 3.1% G: 37.1% T: 11.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 669 90.9 0 669 4 65 22.7 0 65 6 1 1.4 0 1