This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.94 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 36,606 (1.2%) Reads that were too short: 21,595 (0.7%) Reads written (passing filters): 15,011 (0.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 3,137,334 bp (0.3%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 36606 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 74 755.5 0 74 7 3876 755.5 0 3876 8 18 755.5 0 18 9 65 755.5 0 65 10 29 755.5 0 29 11 17 755.5 0 17 12 20 755.5 0 20 13 15 755.5 0 15 14 21 755.5 0 21 15 21 755.5 0 21 16 16 755.5 0 16 17 13 755.5 0 13 18 15 755.5 0 15 19 13 755.5 0 13 20 27 755.5 0 27 21 14 755.5 0 14 22 14 755.5 0 14 23 11 755.5 0 11 24 17 755.5 0 17 25 8 755.5 0 8 26 18 755.5 0 18 27 15 755.5 0 15 28 17 755.5 0 17 29 31 755.5 0 31 30 36 755.5 0 36 31 12 755.5 0 12 32 15 755.5 0 15 33 14 755.5 0 14 34 18 755.5 0 18 35 14 755.5 0 14 36 11 755.5 0 11 37 11 755.5 0 11 38 11 755.5 0 11 39 23 755.5 0 23 40 22 755.5 0 22 41 14 755.5 0 14 42 12 755.5 0 12 43 24 755.5 0 24 44 12 755.5 0 12 45 13 755.5 0 13 46 10 755.5 0 10 47 17 755.5 0 17 48 13 755.5 0 13 49 24 755.5 0 24 50 20 755.5 0 20 51 23 755.5 0 23 52 36 755.5 0 36 53 17 755.5 0 17 54 23 755.5 0 23 55 65 755.5 0 65 56 75 755.5 0 75 57 64 755.5 0 64 58 27 755.5 0 27 59 24 755.5 0 24 60 16 755.5 0 16 61 24 755.5 0 24 62 26 755.5 0 26 63 17 755.5 0 17 64 20 755.5 0 20 65 5 755.5 0 5 66 12 755.5 0 12 67 13 755.5 0 13 68 8 755.5 0 8 69 10 755.5 0 10 70 11 755.5 0 11 71 27 755.5 0 27 72 19 755.5 0 19 73 8 755.5 0 8 74 14 755.5 0 14 75 14 755.5 0 14 76 15 755.5 0 15 77 23 755.5 0 23 78 20 755.5 0 20 79 63 755.5 0 63 80 89 755.5 0 89 81 61 755.5 0 61 82 37 755.5 0 37 83 302 755.5 0 302 84 58 755.5 0 58 85 24 755.5 0 24 86 12 755.5 0 12 87 15 755.5 0 15 88 13 755.5 0 13 89 13 755.5 0 13 90 22 755.5 0 22 91 10 755.5 0 10 92 6 755.5 0 6 93 23 755.5 0 23 94 20 755.5 0 20 95 15 755.5 0 15 96 13 755.5 0 13 97 16 755.5 0 16 98 27 755.5 0 27 99 73 755.5 0 73 100 31 755.5 0 31 101 27 755.5 0 27 102 15 755.5 0 15 103 22 755.5 0 22 104 15 755.5 0 15 105 19 755.5 0 19 106 32 755.5 0 32 107 29 755.5 0 29 108 21 755.5 0 21 109 36 755.5 0 36 110 35 755.5 0 35 111 29 755.5 0 29 112 43 755.5 0 43 113 106 755.5 0 106 114 1122 755.5 0 1122 115 2574 755.5 0 2574 116 22 755.5 0 22 117 14 755.5 0 14 118 14 755.5 0 14 119 11 755.5 0 11 120 15 755.5 0 15 121 9 755.5 0 9 122 16 755.5 0 16 123 20 755.5 0 20 124 16 755.5 0 16 125 12 755.5 0 12 126 37 755.5 0 37 127 12 755.5 0 12 128 18 755.5 0 18 129 38 755.5 0 38 130 44 755.5 0 44 131 22 755.5 0 22 132 29 755.5 0 29 133 15 755.5 0 15 134 23 755.5 0 23 135 32 755.5 0 32 136 18 755.5 0 18 137 18 755.5 0 18 138 20 755.5 0 20 139 25 755.5 0 25 140 30 755.5 0 30 141 36 755.5 0 36 142 22 755.5 0 22 143 104 755.5 0 104 144 89 755.5 0 89 145 32 755.5 0 32 146 16 755.5 0 16 147 16 755.5 0 16 148 12 755.5 0 12 149 16 755.5 0 16 150 62 755.5 0 62 151 9 755.5 0 9 152 25 755.5 0 25 153 12 755.5 0 12 154 28 755.5 0 28 155 86 755.5 0 86 156 192 755.5 0 192 157 125 755.5 0 125 158 90 755.5 0 90 159 14 755.5 0 14 160 20 755.5 0 20 161 19 755.5 0 19 162 20 755.5 0 20 163 42 755.5 0 42 164 29 755.5 0 29 165 67 755.5 0 67 166 68 755.5 0 68 167 902 755.5 0 902 168 97 755.5 0 97 169 1150 755.5 0 1150 170 18 755.5 0 18 171 18 755.5 0 18 172 12 755.5 0 12 173 12 755.5 0 12 174 16 755.5 0 16 175 3 755.5 0 3 176 9 755.5 0 9 177 9 755.5 0 9 178 11 755.5 0 11 179 12 755.5 0 12 180 20 755.5 0 20 181 24 755.5 0 24 182 12 755.5 0 12 183 21 755.5 0 21 184 43 755.5 0 43 185 14 755.5 0 14 186 14 755.5 0 14 187 19 755.5 0 19 188 87 755.5 0 87 189 10 755.5 0 10 190 16 755.5 0 16 191 25 755.5 0 25 192 24 755.5 0 24 193 11 755.5 0 11 194 7 755.5 0 7 195 11 755.5 0 11 196 16 755.5 0 16 197 11 755.5 0 11 198 18 755.5 0 18 199 10 755.5 0 10 200 70 755.5 0 70 201 23 755.5 0 23 202 38 755.5 0 38 203 34 755.5 0 34 204 45 755.5 0 45 205 13 755.5 0 13 206 15 755.5 0 15 207 19 755.5 0 19 208 11 755.5 0 11 209 8 755.5 0 8 210 57 755.5 0 57 211 33 755.5 0 33 212 54 755.5 0 54 213 202 755.5 0 202 214 30 755.5 0 30 215 18 755.5 0 18 216 36 755.5 0 36 217 74 755.5 0 74 218 18 755.5 0 18 219 4 755.5 0 4 220 3 755.5 0 3 221 12 755.5 0 12 222 13 755.5 0 13 223 13 755.5 0 13 224 29 755.5 0 29 225 18 755.5 0 18 226 21 755.5 0 21 227 27 755.5 0 27 228 25 755.5 0 25 229 36 755.5 0 36 230 79 755.5 0 79 231 21 755.5 0 21 232 25 755.5 0 25 233 27 755.5 0 27 234 28 755.5 0 28 235 26 755.5 0 26 236 21 755.5 0 21 237 31 755.5 0 31 238 44 755.5 0 44 239 15 755.5 0 15 240 6 755.5 0 6 241 10 755.5 0 10 242 19 755.5 0 19 243 22 755.5 0 22 244 21 755.5 0 21 245 42 755.5 0 42 246 6 755.5 0 6 247 20 755.5 0 20 248 22 755.5 0 22 249 7 755.5 0 7 250 18 755.5 0 18 251 34 755.5 0 34 252 28 755.5 0 28 253 51 755.5 0 51 254 48 755.5 0 48 255 100 755.5 0 100 256 201 755.5 0 201 257 61 755.5 0 61 258 32 755.5 0 32 259 33 755.5 0 33 260 15 755.5 0 15 261 13 755.5 0 13 262 25 755.5 0 25 263 26 755.5 0 26 264 35 755.5 0 35 265 22 755.5 0 22 266 28 755.5 0 28 267 11 755.5 0 11 268 12 755.5 0 12 269 28 755.5 0 28 270 28 755.5 0 28 271 47 755.5 0 47 272 21 755.5 0 21 273 33 755.5 0 33 274 38 755.5 0 38 275 10 755.5 0 10 276 18 755.5 0 18 277 43 755.5 0 43 278 101 755.5 0 101 279 88 755.5 0 88 280 47 755.5 0 47 281 22 755.5 0 22 282 37 755.5 0 37 283 53 755.5 0 53 284 86 755.5 0 86 285 94 755.5 0 94 286 64 755.5 0 64 287 36 755.5 0 36 288 68 755.5 0 68 289 60 755.5 0 60 290 59 755.5 0 59 291 163 755.5 0 163 292 242 755.5 0 242 293 285 755.5 0 285 294 299 755.5 0 299 295 915 755.5 0 915 296 3538 755.5 0 3538 297 5859 755.5 0 5859 298 3827 755.5 0 3827 299 1415 755.5 0 1415 300 1720 755.5 0 1720 301 60 755.5 0 60 Finished in 0.66 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 15,011 Reads with adapters: 3,889 (25.9%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 3,883 (25.9%) Total basepairs processed: 3,137,334 bp Total written (filtered): 1,073,859 bp (34.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3889 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 1 1 16 16 0.0 3 0 12 1 3 17 3799 0.0 3 3676 107 14 2 18 18 0.0 3 0 11 3 4 19 31 0.0 3 11 3 3 14 20 3 0.0 3 0 0 0 3 21 1 0.0 3 0 0 1 23 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 3,883 Reads with adapters: 437 (11.3%) Reads written (passing filters): 3,883 (100.0%) Total basepairs processed: 1,073,859 bp Total written (filtered): 1,072,500 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 437 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.7% C: 46.7% G: 15.8% T: 17.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 389 60.7 0 389 4 48 15.2 0 48