This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.42 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 51,021 (1.6%) Reads that were too short: 23,052 (0.7%) Reads written (passing filters): 27,969 (0.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 5,779,610 bp (0.6%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 51021 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 35 755.5 0 35 7 60 755.5 0 60 8 77 755.5 0 77 9 3204 755.5 0 3204 10 28 755.5 0 28 11 30 755.5 0 30 12 18 755.5 0 18 13 36 755.5 0 36 14 25 755.5 0 25 15 21 755.5 0 21 16 23 755.5 0 23 17 28 755.5 0 28 18 27 755.5 0 27 19 36 755.5 0 36 20 15 755.5 0 15 21 48 755.5 0 48 22 58 755.5 0 58 23 32 755.5 0 32 24 35 755.5 0 35 25 51 755.5 0 51 26 39 755.5 0 39 27 36 755.5 0 36 28 31 755.5 0 31 29 37 755.5 0 37 30 37 755.5 0 37 31 42 755.5 0 42 32 51 755.5 0 51 33 51 755.5 0 51 34 65 755.5 0 65 35 388 755.5 0 388 36 490 755.5 0 490 37 2168 755.5 0 2168 38 39 755.5 0 39 39 34 755.5 0 34 40 21 755.5 0 21 41 29 755.5 0 29 42 26 755.5 0 26 43 37 755.5 0 37 44 26 755.5 0 26 45 29 755.5 0 29 46 24 755.5 0 24 47 30 755.5 0 30 48 16 755.5 0 16 49 22 755.5 0 22 50 32 755.5 0 32 51 23 755.5 0 23 52 36 755.5 0 36 53 35 755.5 0 35 54 24 755.5 0 24 55 25 755.5 0 25 56 35 755.5 0 35 57 20 755.5 0 20 58 25 755.5 0 25 59 33 755.5 0 33 60 29 755.5 0 29 61 27 755.5 0 27 62 30 755.5 0 30 63 28 755.5 0 28 64 24 755.5 0 24 65 21 755.5 0 21 66 15 755.5 0 15 67 32 755.5 0 32 68 48 755.5 0 48 69 353 755.5 0 353 70 27 755.5 0 27 71 25 755.5 0 25 72 66 755.5 0 66 73 25 755.5 0 25 74 42 755.5 0 42 75 48 755.5 0 48 76 112 755.5 0 112 77 70 755.5 0 70 78 112 755.5 0 112 79 53 755.5 0 53 80 139 755.5 0 139 81 105 755.5 0 105 82 117 755.5 0 117 83 56 755.5 0 56 84 70 755.5 0 70 85 48 755.5 0 48 86 39 755.5 0 39 87 129 755.5 0 129 88 125 755.5 0 125 89 266 755.5 0 266 90 1149 755.5 0 1149 91 63 755.5 0 63 92 123 755.5 0 123 93 122 755.5 0 122 94 99 755.5 0 99 95 98 755.5 0 98 96 184 755.5 0 184 97 497 755.5 0 497 98 935 755.5 0 935 99 2470 755.5 0 2470 100 1032 755.5 0 1032 101 331 755.5 0 331 102 73 755.5 0 73 103 47 755.5 0 47 104 160 755.5 0 160 105 67 755.5 0 67 106 87 755.5 0 87 107 99 755.5 0 99 108 67 755.5 0 67 109 125 755.5 0 125 110 281 755.5 0 281 111 321 755.5 0 321 112 665 755.5 0 665 113 91 755.5 0 91 114 67 755.5 0 67 115 78 755.5 0 78 116 68 755.5 0 68 117 55 755.5 0 55 118 93 755.5 0 93 119 60 755.5 0 60 120 86 755.5 0 86 121 147 755.5 0 147 122 144 755.5 0 144 123 78 755.5 0 78 124 100 755.5 0 100 125 78 755.5 0 78 126 57 755.5 0 57 127 119 755.5 0 119 128 106 755.5 0 106 129 149 755.5 0 149 130 102 755.5 0 102 131 121 755.5 0 121 132 144 755.5 0 144 133 125 755.5 0 125 134 105 755.5 0 105 135 117 755.5 0 117 136 87 755.5 0 87 137 57 755.5 0 57 138 67 755.5 0 67 139 150 755.5 0 150 140 109 755.5 0 109 141 109 755.5 0 109 142 162 755.5 0 162 143 69 755.5 0 69 144 88 755.5 0 88 145 72 755.5 0 72 146 68 755.5 0 68 147 93 755.5 0 93 148 143 755.5 0 143 149 137 755.5 0 137 150 83 755.5 0 83 151 46 755.5 0 46 152 50 755.5 0 50 153 55 755.5 0 55 154 100 755.5 0 100 155 133 755.5 0 133 156 110 755.5 0 110 157 89 755.5 0 89 158 85 755.5 0 85 159 232 755.5 0 232 160 82 755.5 0 82 161 215 755.5 0 215 162 86 755.5 0 86 163 57 755.5 0 57 164 87 755.5 0 87 165 79 755.5 0 79 166 84 755.5 0 84 167 107 755.5 0 107 168 177 755.5 0 177 169 77 755.5 0 77 170 84 755.5 0 84 171 76 755.5 0 76 172 69 755.5 0 69 173 71 755.5 0 71 174 62 755.5 0 62 175 57 755.5 0 57 176 48 755.5 0 48 177 73 755.5 0 73 178 68 755.5 0 68 179 65 755.5 0 65 180 68 755.5 0 68 181 54 755.5 0 54 182 77 755.5 0 77 183 75 755.5 0 75 184 71 755.5 0 71 185 95 755.5 0 95 186 71 755.5 0 71 187 71 755.5 0 71 188 68 755.5 0 68 189 102 755.5 0 102 190 93 755.5 0 93 191 94 755.5 0 94 192 92 755.5 0 92 193 128 755.5 0 128 194 85 755.5 0 85 195 174 755.5 0 174 196 109 755.5 0 109 197 217 755.5 0 217 198 88 755.5 0 88 199 59 755.5 0 59 200 133 755.5 0 133 201 218 755.5 0 218 202 242 755.5 0 242 203 248 755.5 0 248 204 120 755.5 0 120 205 215 755.5 0 215 206 388 755.5 0 388 207 386 755.5 0 386 208 240 755.5 0 240 209 233 755.5 0 233 210 93 755.5 0 93 211 131 755.5 0 131 212 127 755.5 0 127 213 97 755.5 0 97 214 156 755.5 0 156 215 96 755.5 0 96 216 99 755.5 0 99 217 107 755.5 0 107 218 52 755.5 0 52 219 70 755.5 0 70 220 117 755.5 0 117 221 170 755.5 0 170 222 128 755.5 0 128 223 160 755.5 0 160 224 112 755.5 0 112 225 83 755.5 0 83 226 81 755.5 0 81 227 107 755.5 0 107 228 84 755.5 0 84 229 113 755.5 0 113 230 117 755.5 0 117 231 175 755.5 0 175 232 130 755.5 0 130 233 147 755.5 0 147 234 172 755.5 0 172 235 302 755.5 0 302 236 1154 755.5 0 1154 237 827 755.5 0 827 238 117 755.5 0 117 239 203 755.5 0 203 240 233 755.5 0 233 241 967 755.5 0 967 242 360 755.5 0 360 243 135 755.5 0 135 244 56 755.5 0 56 245 63 755.5 0 63 246 76 755.5 0 76 247 54 755.5 0 54 248 99 755.5 0 99 249 168 755.5 0 168 250 284 755.5 0 284 251 115 755.5 0 115 252 65 755.5 0 65 253 40 755.5 0 40 254 45 755.5 0 45 255 48 755.5 0 48 256 42 755.5 0 42 257 69 755.5 0 69 258 107 755.5 0 107 259 201 755.5 0 201 260 612 755.5 0 612 261 1883 755.5 0 1883 262 2473 755.5 0 2473 263 276 755.5 0 276 264 86 755.5 0 86 265 122 755.5 0 122 266 84 755.5 0 84 267 54 755.5 0 54 268 75 755.5 0 75 269 89 755.5 0 89 270 157 755.5 0 157 271 128 755.5 0 128 272 127 755.5 0 127 273 117 755.5 0 117 274 255 755.5 0 255 275 655 755.5 0 655 276 133 755.5 0 133 277 82 755.5 0 82 278 221 755.5 0 221 279 103 755.5 0 103 280 261 755.5 0 261 281 122 755.5 0 122 282 253 755.5 0 253 283 125 755.5 0 125 284 69 755.5 0 69 285 83 755.5 0 83 286 115 755.5 0 115 287 108 755.5 0 108 288 200 755.5 0 200 289 190 755.5 0 190 290 189 755.5 0 189 291 114 755.5 0 114 292 109 755.5 0 109 293 86 755.5 0 86 294 130 755.5 0 130 295 131 755.5 0 131 296 160 755.5 0 160 297 290 755.5 0 290 298 434 755.5 0 434 299 216 755.5 0 216 300 359 755.5 0 359 301 750 755.5 0 750 Finished in 1.25 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 27,969 Reads with adapters: 3,315 (11.9%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 3,303 (11.8%) Total basepairs processed: 5,779,610 bp Total written (filtered): 899,089 bp (15.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3315 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 42 0.0 3 0 39 3 17 3177 0.0 3 3084 79 11 3 18 10 0.0 3 0 4 5 1 19 2 0.0 3 0 0 2 20 10 0.0 3 1 0 0 9 21 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 84 4 0.0 3 0 0 0 4 86 6 0.0 3 0 0 0 6 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 131 6 0.0 3 0 0 0 6 132 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 146 3 0.0 3 0 0 0 3 147 4 0.0 3 0 0 0 4 148 16 0.0 3 0 0 0 16 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 1 1 169 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 1 193 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,303 Reads with adapters: 297 (9.0%) Reads written (passing filters): 3,303 (100.0%) Total basepairs processed: 899,089 bp Total written (filtered): 898,149 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 297 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.3% C: 15.8% G: 10.8% T: 13.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 253 51.6 0 253 4 42 12.9 0 42 5 1 3.2 0 1 8 1 0.1 0 1