This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.67 s (31 us/read; 1.92 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 471,939 (15.3%) Reads that were too short: 457,896 (14.8%) Reads written (passing filters): 14,043 (0.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 3,206,345 bp (0.3%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 471939 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 117 755.5 0 117 7 140 755.5 0 140 8 6551 755.5 0 6551 9 36 755.5 0 36 10 34 755.5 0 34 11 18 755.5 0 18 12 14 755.5 0 14 13 26 755.5 0 26 14 24 755.5 0 24 15 17 755.5 0 17 16 25 755.5 0 25 17 25 755.5 0 25 18 32 755.5 0 32 19 25 755.5 0 25 20 60 755.5 0 60 21 27 755.5 0 27 22 32 755.5 0 32 23 24 755.5 0 24 24 38 755.5 0 38 25 17 755.5 0 17 26 33 755.5 0 33 27 14 755.5 0 14 28 24 755.5 0 24 29 26 755.5 0 26 30 19 755.5 0 19 31 12 755.5 0 12 32 29 755.5 0 29 33 20 755.5 0 20 34 16 755.5 0 16 35 17 755.5 0 17 36 29 755.5 0 29 37 20 755.5 0 20 38 23 755.5 0 23 39 37 755.5 0 37 40 14 755.5 0 14 41 17 755.5 0 17 42 25 755.5 0 25 43 25 755.5 0 25 44 20 755.5 0 20 45 9 755.5 0 9 46 15 755.5 0 15 47 26 755.5 0 26 48 27 755.5 0 27 49 13 755.5 0 13 50 20 755.5 0 20 51 23 755.5 0 23 52 17 755.5 0 17 53 98 755.5 0 98 54 43 755.5 0 43 55 23 755.5 0 23 56 13 755.5 0 13 57 30 755.5 0 30 58 10 755.5 0 10 59 15 755.5 0 15 60 13 755.5 0 13 61 7 755.5 0 7 62 17 755.5 0 17 63 28 755.5 0 28 64 36 755.5 0 36 65 22 755.5 0 22 66 28 755.5 0 28 67 29 755.5 0 29 68 18 755.5 0 18 69 25 755.5 0 25 70 20 755.5 0 20 71 22 755.5 0 22 72 36 755.5 0 36 73 24 755.5 0 24 74 25 755.5 0 25 75 22 755.5 0 22 76 31 755.5 0 31 77 38 755.5 0 38 78 33 755.5 0 33 79 25 755.5 0 25 80 21 755.5 0 21 81 14 755.5 0 14 82 18 755.5 0 18 83 29 755.5 0 29 84 23 755.5 0 23 85 24 755.5 0 24 86 14 755.5 0 14 87 14 755.5 0 14 88 22 755.5 0 22 89 36 755.5 0 36 90 29 755.5 0 29 91 33 755.5 0 33 92 21 755.5 0 21 93 58 755.5 0 58 94 17 755.5 0 17 95 11 755.5 0 11 96 19 755.5 0 19 97 14 755.5 0 14 98 10 755.5 0 10 99 21 755.5 0 21 100 9 755.5 0 9 101 16 755.5 0 16 102 32 755.5 0 32 103 18 755.5 0 18 104 18 755.5 0 18 105 21 755.5 0 21 106 9 755.5 0 9 107 13 755.5 0 13 108 17 755.5 0 17 109 26 755.5 0 26 110 13 755.5 0 13 111 13 755.5 0 13 112 19 755.5 0 19 113 16 755.5 0 16 114 12 755.5 0 12 115 9 755.5 0 9 116 19 755.5 0 19 117 15 755.5 0 15 118 10 755.5 0 10 119 25 755.5 0 25 120 18 755.5 0 18 121 21 755.5 0 21 122 37 755.5 0 37 123 25 755.5 0 25 124 26 755.5 0 26 125 19 755.5 0 19 126 39 755.5 0 39 127 26 755.5 0 26 128 21 755.5 0 21 129 22 755.5 0 22 130 31 755.5 0 31 131 36 755.5 0 36 132 267 755.5 0 267 133 47 755.5 0 47 134 24 755.5 0 24 135 21 755.5 0 21 136 21 755.5 0 21 137 24 755.5 0 24 138 26 755.5 0 26 139 33 755.5 0 33 140 14 755.5 0 14 141 24 755.5 0 24 142 17 755.5 0 17 143 19 755.5 0 19 144 23 755.5 0 23 145 26 755.5 0 26 146 27 755.5 0 27 147 31 755.5 0 31 148 40 755.5 0 40 149 19 755.5 0 19 150 15 755.5 0 15 151 29 755.5 0 29 152 24 755.5 0 24 153 23 755.5 0 23 154 28 755.5 0 28 155 34 755.5 0 34 156 35 755.5 0 35 157 31 755.5 0 31 158 28 755.5 0 28 159 17 755.5 0 17 160 26 755.5 0 26 161 23 755.5 0 23 162 33 755.5 0 33 163 30 755.5 0 30 164 31 755.5 0 31 165 30 755.5 0 30 166 40 755.5 0 40 167 41 755.5 0 41 168 41 755.5 0 41 169 23 755.5 0 23 170 32 755.5 0 32 171 34 755.5 0 34 172 28 755.5 0 28 173 134 755.5 0 134 174 72 755.5 0 72 175 46 755.5 0 46 176 27 755.5 0 27 177 19 755.5 0 19 178 19 755.5 0 19 179 25 755.5 0 25 180 55 755.5 0 55 181 87 755.5 0 87 182 50 755.5 0 50 183 34 755.5 0 34 184 32 755.5 0 32 185 194 755.5 0 194 186 84 755.5 0 84 187 51 755.5 0 51 188 42 755.5 0 42 189 49 755.5 0 49 190 46 755.5 0 46 191 58 755.5 0 58 192 143 755.5 0 143 193 705 755.5 0 705 194 216 755.5 0 216 195 495 755.5 0 495 196 42 755.5 0 42 197 44 755.5 0 44 198 39 755.5 0 39 199 39 755.5 0 39 200 43 755.5 0 43 201 92 755.5 0 92 202 42 755.5 0 42 203 30 755.5 0 30 204 32 755.5 0 32 205 34 755.5 0 34 206 52 755.5 0 52 207 60 755.5 0 60 208 59 755.5 0 59 209 35 755.5 0 35 210 38 755.5 0 38 211 43 755.5 0 43 212 57 755.5 0 57 213 88 755.5 0 88 214 75 755.5 0 75 215 113 755.5 0 113 216 76 755.5 0 76 217 132 755.5 0 132 218 191 755.5 0 191 219 431 755.5 0 431 220 128 755.5 0 128 221 228 755.5 0 228 222 484 755.5 0 484 223 361 755.5 0 361 224 656 755.5 0 656 225 520 755.5 0 520 226 429 755.5 0 429 227 652 755.5 0 652 228 561 755.5 0 561 229 667 755.5 0 667 230 971 755.5 0 971 231 1390 755.5 0 1390 232 13588 755.5 0 13588 233 12454 755.5 0 12454 234 37550 755.5 0 37550 235 995 755.5 0 995 236 848 755.5 0 848 237 6670 755.5 0 6670 238 11036 755.5 0 11036 239 72773 755.5 0 72773 240 919 755.5 0 919 241 479 755.5 0 479 242 496 755.5 0 496 243 1738 755.5 0 1738 244 1358 755.5 0 1358 245 420 755.5 0 420 246 429 755.5 0 429 247 1090 755.5 0 1090 248 665 755.5 0 665 249 698 755.5 0 698 250 1201 755.5 0 1201 251 2662 755.5 0 2662 252 2070 755.5 0 2070 253 3028 755.5 0 3028 254 3553 755.5 0 3553 255 1942 755.5 0 1942 256 2339 755.5 0 2339 257 16459 755.5 0 16459 258 32554 755.5 0 32554 259 207158 755.5 0 207158 260 10931 755.5 0 10931 261 78 755.5 0 78 262 26 755.5 0 26 263 22 755.5 0 22 264 18 755.5 0 18 265 23 755.5 0 23 266 17 755.5 0 17 267 10 755.5 0 10 268 27 755.5 0 27 269 11 755.5 0 11 270 34 755.5 0 34 271 12 755.5 0 12 272 17 755.5 0 17 273 20 755.5 0 20 274 9 755.5 0 9 275 6 755.5 0 6 276 14 755.5 0 14 277 14 755.5 0 14 278 14 755.5 0 14 279 17 755.5 0 17 280 21 755.5 0 21 281 11 755.5 0 11 282 14 755.5 0 14 283 11 755.5 0 11 284 9 755.5 0 9 285 24 755.5 0 24 286 38 755.5 0 38 287 122 755.5 0 122 288 14 755.5 0 14 289 23 755.5 0 23 290 86 755.5 0 86 291 196 755.5 0 196 292 75 755.5 0 75 293 30 755.5 0 30 294 20 755.5 0 20 295 8 755.5 0 8 296 9 755.5 0 9 297 11 755.5 0 11 298 9 755.5 0 9 299 10 755.5 0 10 300 12 755.5 0 12 301 16 755.5 0 16 Finished in 0.79 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 14,043 Reads with adapters: 83 (0.6%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 82 (0.6%) Total basepairs processed: 3,206,345 bp Total written (filtered): 16,563 bp (0.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 22 28 0.0 4 27 1 60 1 0.0 4 0 0 0 0 1 127 52 0.0 4 0 0 0 0 52 153 1 0.0 4 0 0 0 0 1 252 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (122 us/read; 0.49 M reads/minute). === Summary === Total reads processed: 82 Reads with adapters: 1 (1.2%) Reads written (passing filters): 82 (100.0%) Total basepairs processed: 16,563 bp Total written (filtered): 16,552 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1