This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.52 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 521,104 (16.8%) Reads that were too short: 316,201 (10.2%) Reads written (passing filters): 204,903 (6.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 24,696,827 bp (2.7%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 521104 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 57 755.5 0 57 7 38 755.5 0 38 8 26 755.5 0 26 9 105 755.5 0 105 10 56 755.5 0 56 11 40 755.5 0 40 12 17 755.5 0 17 13 21 755.5 0 21 14 20 755.5 0 20 15 17 755.5 0 17 16 25 755.5 0 25 17 108 755.5 0 108 18 26 755.5 0 26 19 15 755.5 0 15 20 26 755.5 0 26 21 28 755.5 0 28 22 24 755.5 0 24 23 12 755.5 0 12 24 15 755.5 0 15 25 23 755.5 0 23 26 19 755.5 0 19 27 13 755.5 0 13 28 17 755.5 0 17 29 20 755.5 0 20 30 13 755.5 0 13 31 156 755.5 0 156 32 127 755.5 0 127 33 22 755.5 0 22 34 30 755.5 0 30 35 26 755.5 0 26 36 27 755.5 0 27 37 18 755.5 0 18 38 30 755.5 0 30 39 31 755.5 0 31 40 20 755.5 0 20 41 27 755.5 0 27 42 20 755.5 0 20 43 25 755.5 0 25 44 19 755.5 0 19 45 38 755.5 0 38 46 25 755.5 0 25 47 37 755.5 0 37 48 22 755.5 0 22 49 23 755.5 0 23 50 18 755.5 0 18 51 23 755.5 0 23 52 22 755.5 0 22 53 23 755.5 0 23 54 22 755.5 0 22 55 37 755.5 0 37 56 45 755.5 0 45 57 58 755.5 0 58 58 28 755.5 0 28 59 40 755.5 0 40 60 31 755.5 0 31 61 28 755.5 0 28 62 28 755.5 0 28 63 27 755.5 0 27 64 32 755.5 0 32 65 49 755.5 0 49 66 51 755.5 0 51 67 22 755.5 0 22 68 13 755.5 0 13 69 14 755.5 0 14 70 35 755.5 0 35 71 19 755.5 0 19 72 21 755.5 0 21 73 15 755.5 0 15 74 13 755.5 0 13 75 84 755.5 0 84 76 60 755.5 0 60 77 44 755.5 0 44 78 18 755.5 0 18 79 23 755.5 0 23 80 11 755.5 0 11 81 11 755.5 0 11 82 16 755.5 0 16 83 15 755.5 0 15 84 11 755.5 0 11 85 19 755.5 0 19 86 22 755.5 0 22 87 13 755.5 0 13 88 22 755.5 0 22 89 17 755.5 0 17 90 28 755.5 0 28 91 31 755.5 0 31 92 23 755.5 0 23 93 28 755.5 0 28 94 28 755.5 0 28 95 16 755.5 0 16 96 29 755.5 0 29 97 11 755.5 0 11 98 23 755.5 0 23 99 29 755.5 0 29 100 42 755.5 0 42 101 22 755.5 0 22 102 28 755.5 0 28 103 29 755.5 0 29 104 18 755.5 0 18 105 21 755.5 0 21 106 27 755.5 0 27 107 23 755.5 0 23 108 22 755.5 0 22 109 26 755.5 0 26 110 22 755.5 0 22 111 29 755.5 0 29 112 43 755.5 0 43 113 358 755.5 0 358 114 941 755.5 0 941 115 755 755.5 0 755 116 27 755.5 0 27 117 25 755.5 0 25 118 8 755.5 0 8 119 12 755.5 0 12 120 19 755.5 0 19 121 34 755.5 0 34 122 28 755.5 0 28 123 43 755.5 0 43 124 30 755.5 0 30 125 16 755.5 0 16 126 25 755.5 0 25 127 79 755.5 0 79 128 65 755.5 0 65 129 47 755.5 0 47 130 111 755.5 0 111 131 40 755.5 0 40 132 69 755.5 0 69 133 162 755.5 0 162 134 416 755.5 0 416 135 96 755.5 0 96 136 150 755.5 0 150 137 317 755.5 0 317 138 127 755.5 0 127 139 183 755.5 0 183 140 313 755.5 0 313 141 220 755.5 0 220 142 233 755.5 0 233 143 316 755.5 0 316 144 594 755.5 0 594 145 729 755.5 0 729 146 695 755.5 0 695 147 15122 755.5 0 15122 148 7514 755.5 0 7514 149 33512 755.5 0 33512 150 145 755.5 0 145 151 18 755.5 0 18 152 16 755.5 0 16 153 19 755.5 0 19 154 15 755.5 0 15 155 17 755.5 0 17 156 24 755.5 0 24 157 27 755.5 0 27 158 28 755.5 0 28 159 23 755.5 0 23 160 15 755.5 0 15 161 21 755.5 0 21 162 28 755.5 0 28 163 36 755.5 0 36 164 32 755.5 0 32 165 17 755.5 0 17 166 20 755.5 0 20 167 27 755.5 0 27 168 37 755.5 0 37 169 27 755.5 0 27 170 260 755.5 0 260 171 41 755.5 0 41 172 39 755.5 0 39 173 41 755.5 0 41 174 35 755.5 0 35 175 73 755.5 0 73 176 68 755.5 0 68 177 38 755.5 0 38 178 39 755.5 0 39 179 44 755.5 0 44 180 68 755.5 0 68 181 108 755.5 0 108 182 202 755.5 0 202 183 157 755.5 0 157 184 217 755.5 0 217 185 443 755.5 0 443 186 1069 755.5 0 1069 187 899 755.5 0 899 188 431 755.5 0 431 189 1320 755.5 0 1320 190 953 755.5 0 953 191 597 755.5 0 597 192 1183 755.5 0 1183 193 1501 755.5 0 1501 194 928 755.5 0 928 195 1142 755.5 0 1142 196 1795 755.5 0 1795 197 2373 755.5 0 2373 198 2428 755.5 0 2428 199 26572 755.5 0 26572 200 31912 755.5 0 31912 201 60702 755.5 0 60702 202 64120 755.5 0 64120 203 1491 755.5 0 1491 204 1359 755.5 0 1359 205 5419 755.5 0 5419 206 15915 755.5 0 15915 207 35258 755.5 0 35258 208 63148 755.5 0 63148 209 3410 755.5 0 3410 210 588 755.5 0 588 211 1019 755.5 0 1019 212 277 755.5 0 277 213 246 755.5 0 246 214 243 755.5 0 243 215 1415 755.5 0 1415 216 240 755.5 0 240 217 390 755.5 0 390 218 380 755.5 0 380 219 1229 755.5 0 1229 220 900 755.5 0 900 221 1064 755.5 0 1064 222 778 755.5 0 778 223 420 755.5 0 420 224 426 755.5 0 426 225 2975 755.5 0 2975 226 6375 755.5 0 6375 227 10362 755.5 0 10362 228 11865 755.5 0 11865 229 284 755.5 0 284 230 106 755.5 0 106 231 10 755.5 0 10 232 11 755.5 0 11 233 5 755.5 0 5 234 9 755.5 0 9 235 5 755.5 0 5 236 12 755.5 0 12 237 18 755.5 0 18 238 27 755.5 0 27 239 13 755.5 0 13 240 19 755.5 0 19 241 12 755.5 0 12 242 17 755.5 0 17 243 11 755.5 0 11 244 84 755.5 0 84 245 146 755.5 0 146 246 125 755.5 0 125 247 84 755.5 0 84 248 144 755.5 0 144 249 327 755.5 0 327 250 98 755.5 0 98 251 141 755.5 0 141 252 246 755.5 0 246 253 261 755.5 0 261 254 379 755.5 0 379 255 1956 755.5 0 1956 256 538 755.5 0 538 257 322 755.5 0 322 258 451 755.5 0 451 259 650 755.5 0 650 260 528 755.5 0 528 261 527 755.5 0 527 262 2349 755.5 0 2349 263 5877 755.5 0 5877 264 67570 755.5 0 67570 265 408 755.5 0 408 266 55 755.5 0 55 267 14 755.5 0 14 268 5 755.5 0 5 269 23 755.5 0 23 270 12 755.5 0 12 271 21 755.5 0 21 272 7 755.5 0 7 273 4 755.5 0 4 274 8 755.5 0 8 275 49 755.5 0 49 276 15 755.5 0 15 277 4 755.5 0 4 278 12 755.5 0 12 279 13 755.5 0 13 280 7 755.5 0 7 281 5 755.5 0 5 282 8 755.5 0 8 283 8 755.5 0 8 284 28 755.5 0 28 285 13 755.5 0 13 286 8 755.5 0 8 287 6 755.5 0 6 288 3 755.5 0 3 289 5 755.5 0 5 290 6 755.5 0 6 291 7 755.5 0 7 292 34 755.5 0 34 293 108 755.5 0 108 294 549 755.5 0 549 295 11 755.5 0 11 296 8 755.5 0 8 297 2 755.5 0 2 298 13 755.5 0 13 299 4 755.5 0 4 300 6 755.5 0 6 301 38 755.5 0 38 Finished in 6.48 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 204,903 Reads with adapters: 160 (0.1%) Reads that were too short: 65 (0.0%) Reads written (passing filters): 95 (0.0%) Total basepairs processed: 24,696,827 bp Total written (filtered): 22,848 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 160 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 1 1 16 9 0.0 3 0 0 0 9 17 9 0.0 3 5 2 0 2 18 28 0.0 3 0 3 22 3 19 24 0.0 3 1 0 2 21 20 2 0.0 3 0 0 0 2 21 2 0.0 3 0 0 0 2 26 16 0.0 3 0 0 1 15 35 1 0.0 3 1 37 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 75 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 24 0.0 3 0 0 0 24 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 17 0.0 3 0 0 0 17 91 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (105 us/read; 0.57 M reads/minute). === Summary === Total reads processed: 95 Reads with adapters: 8 (8.4%) Reads written (passing filters): 95 (100.0%) Total basepairs processed: 22,848 bp Total written (filtered): 22,821 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.5% C: 25.0% G: 0.0% T: 12.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 1.5 0 5 4 3 0.4 0 3