This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.79 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 509,347 (16.5%) Reads that were too short: 5,076 (0.2%) Reads written (passing filters): 504,271 (16.3%) Total basepairs processed: 931,490,553 bp Total written (filtered): 146,712,981 bp (15.8%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 509347 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2830 755.5 0 2830 7 2838 755.5 0 2838 8 11432 755.5 0 11432 9 481219 755.5 0 481219 10 634 755.5 0 634 11 15 755.5 0 15 12 8 755.5 0 8 13 8 755.5 0 8 14 5 755.5 0 5 15 10 755.5 0 10 16 6 755.5 0 6 17 11 755.5 0 11 18 6 755.5 0 6 19 7 755.5 0 7 20 13 755.5 0 13 21 7 755.5 0 7 22 7 755.5 0 7 23 7 755.5 0 7 24 17 755.5 0 17 25 14 755.5 0 14 26 50 755.5 0 50 27 26 755.5 0 26 28 72 755.5 0 72 29 36 755.5 0 36 30 37 755.5 0 37 31 26 755.5 0 26 32 4 755.5 0 4 33 7 755.5 0 7 34 8 755.5 0 8 35 5 755.5 0 5 36 10 755.5 0 10 38 7 755.5 0 7 39 8 755.5 0 8 40 6 755.5 0 6 41 5 755.5 0 5 42 6 755.5 0 6 43 5 755.5 0 5 44 4 755.5 0 4 45 10 755.5 0 10 46 5 755.5 0 5 47 7 755.5 0 7 48 9 755.5 0 9 49 8 755.5 0 8 50 7 755.5 0 7 51 23 755.5 0 23 52 12 755.5 0 12 53 4 755.5 0 4 54 2 755.5 0 2 55 5 755.5 0 5 56 8 755.5 0 8 57 12 755.5 0 12 58 3 755.5 0 3 59 5 755.5 0 5 60 11 755.5 0 11 61 4 755.5 0 4 62 6 755.5 0 6 63 7 755.5 0 7 64 12 755.5 0 12 65 9 755.5 0 9 66 9 755.5 0 9 67 21 755.5 0 21 68 29 755.5 0 29 69 629 755.5 0 629 70 24 755.5 0 24 71 152 755.5 0 152 72 5 755.5 0 5 73 14 755.5 0 14 74 7 755.5 0 7 75 11 755.5 0 11 76 22 755.5 0 22 77 350 755.5 0 350 78 9 755.5 0 9 79 81 755.5 0 81 80 7 755.5 0 7 81 6 755.5 0 6 82 7 755.5 0 7 83 4 755.5 0 4 84 4 755.5 0 4 85 9 755.5 0 9 86 8 755.5 0 8 87 11 755.5 0 11 88 3 755.5 0 3 89 6 755.5 0 6 90 4 755.5 0 4 91 9 755.5 0 9 92 1 755.5 0 1 93 3 755.5 0 3 94 5 755.5 0 5 95 3 755.5 0 3 96 4 755.5 0 4 97 20 755.5 0 20 98 30 755.5 0 30 99 52 755.5 0 52 100 4 755.5 0 4 101 3 755.5 0 3 102 3 755.5 0 3 103 9 755.5 0 9 104 7 755.5 0 7 105 3 755.5 0 3 106 5 755.5 0 5 107 7 755.5 0 7 108 10 755.5 0 10 109 6 755.5 0 6 110 3 755.5 0 3 111 10 755.5 0 10 112 13 755.5 0 13 113 5 755.5 0 5 114 16 755.5 0 16 115 22 755.5 0 22 116 36 755.5 0 36 117 6 755.5 0 6 118 9 755.5 0 9 119 6 755.5 0 6 120 9 755.5 0 9 121 9 755.5 0 9 122 8 755.5 0 8 123 14 755.5 0 14 124 10 755.5 0 10 125 9 755.5 0 9 126 7 755.5 0 7 127 11 755.5 0 11 128 9 755.5 0 9 129 10 755.5 0 10 130 20 755.5 0 20 131 7 755.5 0 7 132 24 755.5 0 24 133 254 755.5 0 254 134 27 755.5 0 27 135 21 755.5 0 21 136 25 755.5 0 25 137 63 755.5 0 63 138 58 755.5 0 58 139 141 755.5 0 141 140 43 755.5 0 43 141 19 755.5 0 19 142 23 755.5 0 23 143 36 755.5 0 36 144 18 755.5 0 18 145 27 755.5 0 27 146 24 755.5 0 24 147 39 755.5 0 39 148 111 755.5 0 111 149 394 755.5 0 394 150 274 755.5 0 274 151 346 755.5 0 346 152 115 755.5 0 115 153 51 755.5 0 51 154 12 755.5 0 12 155 12 755.5 0 12 156 11 755.5 0 11 157 22 755.5 0 22 158 8 755.5 0 8 159 15 755.5 0 15 160 12 755.5 0 12 161 13 755.5 0 13 162 10 755.5 0 10 163 11 755.5 0 11 164 17 755.5 0 17 165 14 755.5 0 14 166 16 755.5 0 16 167 11 755.5 0 11 168 14 755.5 0 14 169 7 755.5 0 7 170 9 755.5 0 9 171 7 755.5 0 7 172 19 755.5 0 19 173 10 755.5 0 10 174 4 755.5 0 4 175 6 755.5 0 6 176 9 755.5 0 9 177 12 755.5 0 12 178 6 755.5 0 6 179 10 755.5 0 10 180 11 755.5 0 11 181 11 755.5 0 11 182 11 755.5 0 11 183 20 755.5 0 20 184 47 755.5 0 47 185 22 755.5 0 22 186 28 755.5 0 28 187 4 755.5 0 4 188 8 755.5 0 8 189 27 755.5 0 27 190 28 755.5 0 28 191 52 755.5 0 52 192 19 755.5 0 19 193 10 755.5 0 10 194 7 755.5 0 7 195 10 755.5 0 10 196 31 755.5 0 31 197 6 755.5 0 6 198 5 755.5 0 5 199 5 755.5 0 5 200 3 755.5 0 3 201 26 755.5 0 26 202 11 755.5 0 11 203 17 755.5 0 17 204 8 755.5 0 8 205 11 755.5 0 11 206 23 755.5 0 23 207 9 755.5 0 9 208 8 755.5 0 8 209 44 755.5 0 44 210 29 755.5 0 29 211 37 755.5 0 37 212 10 755.5 0 10 213 22 755.5 0 22 214 13 755.5 0 13 215 21 755.5 0 21 216 27 755.5 0 27 217 9 755.5 0 9 218 14 755.5 0 14 219 6 755.5 0 6 220 7 755.5 0 7 221 9 755.5 0 9 222 26 755.5 0 26 223 10 755.5 0 10 224 7 755.5 0 7 225 16 755.5 0 16 226 21 755.5 0 21 227 18 755.5 0 18 228 20 755.5 0 20 229 16 755.5 0 16 230 5 755.5 0 5 231 7 755.5 0 7 232 7 755.5 0 7 233 29 755.5 0 29 234 10 755.5 0 10 235 17 755.5 0 17 236 25 755.5 0 25 237 22 755.5 0 22 238 21 755.5 0 21 239 28 755.5 0 28 240 31 755.5 0 31 241 19 755.5 0 19 242 8 755.5 0 8 243 25 755.5 0 25 244 22 755.5 0 22 245 26 755.5 0 26 246 18 755.5 0 18 247 34 755.5 0 34 248 12 755.5 0 12 249 57 755.5 0 57 250 121 755.5 0 121 251 57 755.5 0 57 252 14 755.5 0 14 253 27 755.5 0 27 254 65 755.5 0 65 255 84 755.5 0 84 256 20 755.5 0 20 257 10 755.5 0 10 258 18 755.5 0 18 259 40 755.5 0 40 260 18 755.5 0 18 261 26 755.5 0 26 262 17 755.5 0 17 263 21 755.5 0 21 264 17 755.5 0 17 265 20 755.5 0 20 266 13 755.5 0 13 267 16 755.5 0 16 268 9 755.5 0 9 269 160 755.5 0 160 270 137 755.5 0 137 271 21 755.5 0 21 272 12 755.5 0 12 273 12 755.5 0 12 274 32 755.5 0 32 275 35 755.5 0 35 276 25 755.5 0 25 277 30 755.5 0 30 278 35 755.5 0 35 279 20 755.5 0 20 280 14 755.5 0 14 281 20 755.5 0 20 282 49 755.5 0 49 283 31 755.5 0 31 284 36 755.5 0 36 285 56 755.5 0 56 286 41 755.5 0 41 287 53 755.5 0 53 288 76 755.5 0 76 289 294 755.5 0 294 290 67 755.5 0 67 291 97 755.5 0 97 292 214 755.5 0 214 293 96 755.5 0 96 294 78 755.5 0 78 295 240 755.5 0 240 296 133 755.5 0 133 297 107 755.5 0 107 298 166 755.5 0 166 299 314 755.5 0 314 300 428 755.5 0 428 301 442 755.5 0 442 Finished in 32.18 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 504,271 Reads with adapters: 2,868 (0.6%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 2,850 (0.6%) Total basepairs processed: 146,712,981 bp Total written (filtered): 468,976 bp (0.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2868 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 1 0.0 3 0 0 0 1 20 42 0.0 4 40 2 21 2 0.0 4 0 1 0 1 22 7 0.0 4 7 44 1 0.0 4 0 0 0 0 1 92 1 0.0 4 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 38 0.0 4 0 0 0 0 38 127 2451 0.0 4 0 0 0 0 2451 128 11 0.0 4 0 0 0 0 11 130 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 138 0.0 4 0 0 0 1 137 152 4 0.0 4 0 0 0 0 4 153 148 0.0 4 0 0 0 1 147 193 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 1 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 2,850 Reads with adapters: 5 (0.2%) Reads written (passing filters): 2,850 (100.0%) Total basepairs processed: 468,976 bp Total written (filtered): 467,634 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 40.0% C: 40.0% G: 0.0% T: 0.0% none/other: 20.0% Overview of removed sequences length count expect max.err error counts 265 2 0.0 1 2 268 1 0.0 1 1 270 1 0.0 1 1 274 1 0.0 1 1