This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.57 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 72,920 (2.4%) Reads that were too short: 5,005 (0.2%) Reads written (passing filters): 67,915 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 16,599,216 bp (1.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 72920 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 627 755.5 0 627 7 958 755.5 0 958 8 37329 755.5 0 37329 9 86 755.5 0 86 10 8 755.5 0 8 11 12 755.5 0 12 12 8 755.5 0 8 13 4 755.5 0 4 14 4 755.5 0 4 15 14 755.5 0 14 16 9 755.5 0 9 17 7 755.5 0 7 18 15 755.5 0 15 19 5 755.5 0 5 20 13 755.5 0 13 21 8 755.5 0 8 22 8 755.5 0 8 23 7 755.5 0 7 24 12 755.5 0 12 25 13 755.5 0 13 26 2 755.5 0 2 27 15 755.5 0 15 28 1 755.5 0 1 29 13 755.5 0 13 30 14 755.5 0 14 31 11 755.5 0 11 32 32 755.5 0 32 33 8 755.5 0 8 34 11 755.5 0 11 35 18 755.5 0 18 36 20 755.5 0 20 37 203 755.5 0 203 38 21 755.5 0 21 39 48 755.5 0 48 40 17 755.5 0 17 41 156 755.5 0 156 42 277 755.5 0 277 43 9 755.5 0 9 44 8 755.5 0 8 45 1 755.5 0 1 46 20 755.5 0 20 47 9 755.5 0 9 48 8 755.5 0 8 49 35 755.5 0 35 50 8 755.5 0 8 51 40 755.5 0 40 52 5234 755.5 0 5234 53 46 755.5 0 46 54 35 755.5 0 35 55 6 755.5 0 6 56 8 755.5 0 8 57 15 755.5 0 15 58 9 755.5 0 9 59 8 755.5 0 8 60 20 755.5 0 20 61 38 755.5 0 38 62 11 755.5 0 11 63 4 755.5 0 4 64 5 755.5 0 5 65 39 755.5 0 39 66 21 755.5 0 21 67 13 755.5 0 13 68 9 755.5 0 9 69 3 755.5 0 3 70 4 755.5 0 4 71 16 755.5 0 16 72 72 755.5 0 72 73 281 755.5 0 281 74 2 755.5 0 2 75 3 755.5 0 3 76 4 755.5 0 4 77 4 755.5 0 4 78 6 755.5 0 6 79 7 755.5 0 7 80 12 755.5 0 12 81 22 755.5 0 22 82 13 755.5 0 13 83 9 755.5 0 9 84 66 755.5 0 66 85 29 755.5 0 29 86 63 755.5 0 63 87 8 755.5 0 8 88 10 755.5 0 10 89 12 755.5 0 12 90 47 755.5 0 47 91 37 755.5 0 37 92 30 755.5 0 30 93 45 755.5 0 45 94 64 755.5 0 64 95 74 755.5 0 74 96 5 755.5 0 5 97 4 755.5 0 4 98 6 755.5 0 6 99 6 755.5 0 6 100 4 755.5 0 4 101 4 755.5 0 4 102 1 755.5 0 1 103 11 755.5 0 11 104 11 755.5 0 11 105 17 755.5 0 17 106 11 755.5 0 11 107 16 755.5 0 16 108 31 755.5 0 31 109 22 755.5 0 22 110 32 755.5 0 32 111 26 755.5 0 26 112 102 755.5 0 102 113 18 755.5 0 18 114 5 755.5 0 5 115 10 755.5 0 10 116 10 755.5 0 10 117 78 755.5 0 78 118 23 755.5 0 23 119 13 755.5 0 13 120 23 755.5 0 23 121 23 755.5 0 23 122 24 755.5 0 24 123 20 755.5 0 20 124 27 755.5 0 27 125 26 755.5 0 26 126 40 755.5 0 40 127 241 755.5 0 241 128 46 755.5 0 46 129 71 755.5 0 71 130 19 755.5 0 19 131 33 755.5 0 33 132 279 755.5 0 279 133 35 755.5 0 35 134 31 755.5 0 31 135 26 755.5 0 26 136 22 755.5 0 22 137 35 755.5 0 35 138 41 755.5 0 41 139 69 755.5 0 69 140 85 755.5 0 85 141 89 755.5 0 89 142 241 755.5 0 241 143 452 755.5 0 452 144 11993 755.5 0 11993 145 1632 755.5 0 1632 146 2407 755.5 0 2407 147 20 755.5 0 20 148 16 755.5 0 16 149 276 755.5 0 276 150 21 755.5 0 21 151 156 755.5 0 156 152 62 755.5 0 62 153 45 755.5 0 45 154 29 755.5 0 29 155 43 755.5 0 43 156 95 755.5 0 95 157 63 755.5 0 63 158 36 755.5 0 36 159 55 755.5 0 55 160 20 755.5 0 20 161 19 755.5 0 19 162 20 755.5 0 20 163 14 755.5 0 14 164 19 755.5 0 19 165 31 755.5 0 31 166 44 755.5 0 44 167 21 755.5 0 21 168 17 755.5 0 17 169 13 755.5 0 13 170 32 755.5 0 32 171 55 755.5 0 55 172 95 755.5 0 95 173 87 755.5 0 87 174 17 755.5 0 17 175 6 755.5 0 6 176 16 755.5 0 16 177 46 755.5 0 46 178 37 755.5 0 37 179 150 755.5 0 150 180 39 755.5 0 39 181 67 755.5 0 67 182 22 755.5 0 22 183 11 755.5 0 11 184 16 755.5 0 16 185 45 755.5 0 45 186 15 755.5 0 15 187 11 755.5 0 11 188 11 755.5 0 11 189 21 755.5 0 21 190 178 755.5 0 178 191 51 755.5 0 51 192 213 755.5 0 213 193 17 755.5 0 17 194 18 755.5 0 18 195 16 755.5 0 16 196 31 755.5 0 31 197 55 755.5 0 55 198 231 755.5 0 231 199 34 755.5 0 34 200 14 755.5 0 14 201 11 755.5 0 11 202 11 755.5 0 11 203 11 755.5 0 11 204 46 755.5 0 46 205 39 755.5 0 39 206 14 755.5 0 14 207 13 755.5 0 13 208 32 755.5 0 32 209 8 755.5 0 8 210 5 755.5 0 5 211 11 755.5 0 11 212 12 755.5 0 12 213 19 755.5 0 19 214 16 755.5 0 16 215 8 755.5 0 8 216 13 755.5 0 13 217 26 755.5 0 26 218 22 755.5 0 22 219 15 755.5 0 15 220 15 755.5 0 15 221 12 755.5 0 12 222 12 755.5 0 12 223 10 755.5 0 10 224 18 755.5 0 18 225 31 755.5 0 31 226 39 755.5 0 39 227 38 755.5 0 38 228 37 755.5 0 37 229 88 755.5 0 88 230 57 755.5 0 57 231 17 755.5 0 17 232 20 755.5 0 20 233 14 755.5 0 14 234 13 755.5 0 13 235 25 755.5 0 25 236 173 755.5 0 173 237 47 755.5 0 47 238 27 755.5 0 27 239 14 755.5 0 14 240 50 755.5 0 50 241 16 755.5 0 16 242 58 755.5 0 58 243 19 755.5 0 19 244 7 755.5 0 7 245 66 755.5 0 66 246 122 755.5 0 122 247 53 755.5 0 53 248 20 755.5 0 20 249 13 755.5 0 13 250 22 755.5 0 22 251 31 755.5 0 31 252 68 755.5 0 68 253 75 755.5 0 75 254 85 755.5 0 85 255 76 755.5 0 76 256 113 755.5 0 113 257 11 755.5 0 11 258 12 755.5 0 12 259 11 755.5 0 11 260 51 755.5 0 51 261 89 755.5 0 89 262 242 755.5 0 242 263 38 755.5 0 38 264 35 755.5 0 35 265 41 755.5 0 41 266 24 755.5 0 24 267 33 755.5 0 33 268 24 755.5 0 24 269 17 755.5 0 17 270 19 755.5 0 19 271 23 755.5 0 23 272 85 755.5 0 85 273 11 755.5 0 11 274 11 755.5 0 11 275 10 755.5 0 10 276 7 755.5 0 7 277 34 755.5 0 34 278 20 755.5 0 20 279 11 755.5 0 11 280 15 755.5 0 15 281 12 755.5 0 12 282 15 755.5 0 15 283 93 755.5 0 93 284 178 755.5 0 178 285 110 755.5 0 110 286 58 755.5 0 58 287 12 755.5 0 12 288 16 755.5 0 16 289 18 755.5 0 18 290 258 755.5 0 258 291 645 755.5 0 645 292 398 755.5 0 398 293 140 755.5 0 140 294 138 755.5 0 138 295 52 755.5 0 52 296 35 755.5 0 35 297 17 755.5 0 17 298 4 755.5 0 4 299 4 755.5 0 4 300 1 755.5 0 1 301 5 755.5 0 5 Finished in 3.11 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 67,915 Reads with adapters: 38,674 (56.9%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 38,661 (56.9%) Total basepairs processed: 16,599,216 bp Total written (filtered): 10,670,799 bp (64.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 38674 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 12 0.0 3 0 0 6 6 16 324 0.0 3 0 305 12 7 17 37990 0.0 3 37058 845 76 11 18 102 0.0 3 3 91 6 2 19 189 0.0 3 0 0 165 24 20 33 0.0 3 0 0 0 33 44 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 151 4 0.0 3 0 0 0 4 192 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 4 0.0 3 0 0 0 4 244 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 38,661 Reads with adapters: 9,507 (24.6%) Reads written (passing filters): 38,661 (100.0%) Total basepairs processed: 10,670,799 bp Total written (filtered): 10,641,495 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9507 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.0% C: 0.5% G: 72.4% T: 12.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8732 604.1 0 8732 4 770 151.0 0 770 5 2 37.8 0 2 6 3 9.4 0 3