This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.17 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 77,449 (2.5%) Reads that were too short: 12,987 (0.4%) Reads written (passing filters): 64,462 (2.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 17,327,388 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 77449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 478 755.5 0 478 7 621 755.5 0 621 8 14253 755.5 0 14253 9 37139 755.5 0 37139 10 61 755.5 0 61 11 4 755.5 0 4 12 2 755.5 0 2 13 3 755.5 0 3 15 2 755.5 0 2 16 1 755.5 0 1 17 2 755.5 0 2 18 1 755.5 0 1 19 2 755.5 0 2 20 3 755.5 0 3 21 1 755.5 0 1 22 3 755.5 0 3 23 7 755.5 0 7 24 3 755.5 0 3 25 1 755.5 0 1 26 2 755.5 0 2 27 1 755.5 0 1 28 5 755.5 0 5 30 4 755.5 0 4 31 4 755.5 0 4 32 4 755.5 0 4 33 6 755.5 0 6 34 9 755.5 0 9 35 1 755.5 0 1 36 1 755.5 0 1 37 4 755.5 0 4 38 17 755.5 0 17 39 10 755.5 0 10 40 5 755.5 0 5 41 3 755.5 0 3 42 6 755.5 0 6 43 6 755.5 0 6 44 10 755.5 0 10 45 4 755.5 0 4 46 2 755.5 0 2 47 4 755.5 0 4 48 6 755.5 0 6 49 20 755.5 0 20 50 11 755.5 0 11 51 6 755.5 0 6 52 6 755.5 0 6 53 4 755.5 0 4 54 7 755.5 0 7 55 10 755.5 0 10 56 9 755.5 0 9 57 8 755.5 0 8 58 6 755.5 0 6 59 32 755.5 0 32 60 450 755.5 0 450 61 53 755.5 0 53 62 243 755.5 0 243 63 8 755.5 0 8 64 112 755.5 0 112 65 83 755.5 0 83 66 792 755.5 0 792 67 280 755.5 0 280 68 26 755.5 0 26 69 4 755.5 0 4 70 5 755.5 0 5 71 6 755.5 0 6 72 8 755.5 0 8 73 3 755.5 0 3 74 4 755.5 0 4 75 4 755.5 0 4 76 8 755.5 0 8 77 57 755.5 0 57 78 12 755.5 0 12 79 49 755.5 0 49 80 11 755.5 0 11 81 77 755.5 0 77 82 20 755.5 0 20 83 8 755.5 0 8 84 2 755.5 0 2 85 5 755.5 0 5 86 10 755.5 0 10 87 6 755.5 0 6 88 6 755.5 0 6 89 5 755.5 0 5 90 14 755.5 0 14 91 6 755.5 0 6 92 26 755.5 0 26 93 9 755.5 0 9 94 13 755.5 0 13 95 20 755.5 0 20 96 18 755.5 0 18 97 298 755.5 0 298 98 35 755.5 0 35 99 61 755.5 0 61 100 3 755.5 0 3 101 13 755.5 0 13 102 16 755.5 0 16 103 25 755.5 0 25 104 9 755.5 0 9 105 10 755.5 0 10 106 15 755.5 0 15 107 14 755.5 0 14 108 28 755.5 0 28 109 24 755.5 0 24 110 9 755.5 0 9 111 22 755.5 0 22 112 82 755.5 0 82 113 97 755.5 0 97 114 78 755.5 0 78 115 1162 755.5 0 1162 116 536 755.5 0 536 117 81 755.5 0 81 118 14 755.5 0 14 119 9 755.5 0 9 120 14 755.5 0 14 121 24 755.5 0 24 122 34 755.5 0 34 123 24 755.5 0 24 124 31 755.5 0 31 125 21 755.5 0 21 126 15 755.5 0 15 127 41 755.5 0 41 128 30 755.5 0 30 129 17 755.5 0 17 130 25 755.5 0 25 131 14 755.5 0 14 132 25 755.5 0 25 133 25 755.5 0 25 134 24 755.5 0 24 135 35 755.5 0 35 136 44 755.5 0 44 137 50 755.5 0 50 138 36 755.5 0 36 139 45 755.5 0 45 140 22 755.5 0 22 141 24 755.5 0 24 142 28 755.5 0 28 143 36 755.5 0 36 144 27 755.5 0 27 145 18 755.5 0 18 146 51 755.5 0 51 147 20 755.5 0 20 148 34 755.5 0 34 149 19 755.5 0 19 150 19 755.5 0 19 151 44 755.5 0 44 152 19 755.5 0 19 153 18 755.5 0 18 154 23 755.5 0 23 155 18 755.5 0 18 156 54 755.5 0 54 157 46 755.5 0 46 158 58 755.5 0 58 159 43 755.5 0 43 160 37 755.5 0 37 161 66 755.5 0 66 162 47 755.5 0 47 163 52 755.5 0 52 164 98 755.5 0 98 165 70 755.5 0 70 166 353 755.5 0 353 167 290 755.5 0 290 168 41 755.5 0 41 169 63 755.5 0 63 170 62 755.5 0 62 171 50 755.5 0 50 172 77 755.5 0 77 173 35 755.5 0 35 174 18 755.5 0 18 175 24 755.5 0 24 176 54 755.5 0 54 177 51 755.5 0 51 178 54 755.5 0 54 179 60 755.5 0 60 180 52 755.5 0 52 181 143 755.5 0 143 182 57 755.5 0 57 183 54 755.5 0 54 184 76 755.5 0 76 185 45 755.5 0 45 186 112 755.5 0 112 187 66 755.5 0 66 188 67 755.5 0 67 189 1502 755.5 0 1502 190 438 755.5 0 438 191 563 755.5 0 563 192 61 755.5 0 61 193 59 755.5 0 59 194 117 755.5 0 117 195 40 755.5 0 40 196 72 755.5 0 72 197 48 755.5 0 48 198 29 755.5 0 29 199 30 755.5 0 30 200 41 755.5 0 41 201 69 755.5 0 69 202 104 755.5 0 104 203 102 755.5 0 102 204 59 755.5 0 59 205 56 755.5 0 56 206 47 755.5 0 47 207 50 755.5 0 50 208 51 755.5 0 51 209 49 755.5 0 49 210 35 755.5 0 35 211 51 755.5 0 51 212 70 755.5 0 70 213 72 755.5 0 72 214 64 755.5 0 64 215 51 755.5 0 51 216 68 755.5 0 68 217 108 755.5 0 108 218 60 755.5 0 60 219 71 755.5 0 71 220 91 755.5 0 91 221 56 755.5 0 56 222 53 755.5 0 53 223 17 755.5 0 17 224 28 755.5 0 28 225 69 755.5 0 69 226 81 755.5 0 81 227 73 755.5 0 73 228 81 755.5 0 81 229 59 755.5 0 59 230 62 755.5 0 62 231 79 755.5 0 79 232 118 755.5 0 118 233 85 755.5 0 85 234 67 755.5 0 67 235 75 755.5 0 75 236 230 755.5 0 230 237 196 755.5 0 196 238 121 755.5 0 121 239 78 755.5 0 78 240 76 755.5 0 76 241 53 755.5 0 53 242 47 755.5 0 47 243 57 755.5 0 57 244 73 755.5 0 73 245 943 755.5 0 943 246 2224 755.5 0 2224 247 1992 755.5 0 1992 248 77 755.5 0 77 249 75 755.5 0 75 250 28 755.5 0 28 251 17 755.5 0 17 252 26 755.5 0 26 253 30 755.5 0 30 254 68 755.5 0 68 255 82 755.5 0 82 256 147 755.5 0 147 257 69 755.5 0 69 258 62 755.5 0 62 259 164 755.5 0 164 260 438 755.5 0 438 261 80 755.5 0 80 262 96 755.5 0 96 263 53 755.5 0 53 264 66 755.5 0 66 265 107 755.5 0 107 266 87 755.5 0 87 267 418 755.5 0 418 268 245 755.5 0 245 269 93 755.5 0 93 270 97 755.5 0 97 271 73 755.5 0 73 272 60 755.5 0 60 273 33 755.5 0 33 274 29 755.5 0 29 275 49 755.5 0 49 276 60 755.5 0 60 277 87 755.5 0 87 278 75 755.5 0 75 279 65 755.5 0 65 280 61 755.5 0 61 281 55 755.5 0 55 282 94 755.5 0 94 283 77 755.5 0 77 284 68 755.5 0 68 285 69 755.5 0 69 286 79 755.5 0 79 287 92 755.5 0 92 288 105 755.5 0 105 289 69 755.5 0 69 290 50 755.5 0 50 291 29 755.5 0 29 292 27 755.5 0 27 293 64 755.5 0 64 294 83 755.5 0 83 295 193 755.5 0 193 296 106 755.5 0 106 297 40 755.5 0 40 298 30 755.5 0 30 299 35 755.5 0 35 300 38 755.5 0 38 301 15 755.5 0 15 Finished in 3.41 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 64,462 Reads with adapters: 38,971 (60.5%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 38,963 (60.4%) Total basepairs processed: 17,327,388 bp Total written (filtered): 10,600,923 bp (61.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38971 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 0 4 18 15 0.0 3 0 0 15 19 560 0.0 3 0 549 9 2 20 38173 0.0 4 37617 534 17 2 3 21 80 0.0 4 17 62 1 22 116 0.0 4 109 2 4 1 23 10 0.0 4 0 0 0 8 2 24 1 0.0 4 0 0 0 0 1 26 2 0.0 4 2 27 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 1 81 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.58 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 38,963 Reads with adapters: 1,020 (2.6%) Reads written (passing filters): 38,963 (100.0%) Total basepairs processed: 10,600,923 bp Total written (filtered): 10,566,785 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1020 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.8% C: 0.2% G: 87.2% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 608.8 0 16 4 18 152.2 0 18 5 5 38.0 0 5 6 287 9.5 0 287 9 59 0.1 0 0 59 10 42 0.0 1 2 40 11 493 0.0 1 0 493 257 1 0.0 1 0 1 258 2 0.0 1 2 259 96 0.0 1 93 3 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.