This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 99.80 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 123,782 (4.0%) Reads that were too short: 12,314 (0.4%) Reads written (passing filters): 111,468 (3.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 30,488,110 bp (3.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 123782 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1067 755.5 0 1067 7 86783 755.5 0 86783 8 93 755.5 0 93 9 10 755.5 0 10 10 5 755.5 0 5 11 5 755.5 0 5 12 8 755.5 0 8 13 16 755.5 0 16 14 9 755.5 0 9 15 14 755.5 0 14 16 13 755.5 0 13 17 8 755.5 0 8 18 286 755.5 0 286 19 10 755.5 0 10 20 39 755.5 0 39 21 10 755.5 0 10 22 14 755.5 0 14 23 3 755.5 0 3 24 5 755.5 0 5 25 14 755.5 0 14 26 17 755.5 0 17 27 21 755.5 0 21 28 18 755.5 0 18 29 9 755.5 0 9 30 8 755.5 0 8 31 26 755.5 0 26 32 5 755.5 0 5 33 13 755.5 0 13 34 19 755.5 0 19 35 25 755.5 0 25 36 10 755.5 0 10 37 11 755.5 0 11 38 16 755.5 0 16 39 18 755.5 0 18 40 16 755.5 0 16 41 21 755.5 0 21 42 11 755.5 0 11 43 10 755.5 0 10 44 33 755.5 0 33 45 11 755.5 0 11 46 9 755.5 0 9 47 13 755.5 0 13 48 57 755.5 0 57 49 18 755.5 0 18 50 72 755.5 0 72 51 9 755.5 0 9 52 19 755.5 0 19 53 17 755.5 0 17 54 58 755.5 0 58 55 23 755.5 0 23 56 25 755.5 0 25 57 47 755.5 0 47 58 16 755.5 0 16 59 34 755.5 0 34 60 23 755.5 0 23 61 38 755.5 0 38 62 14 755.5 0 14 63 18 755.5 0 18 64 36 755.5 0 36 65 27 755.5 0 27 66 83 755.5 0 83 67 77 755.5 0 77 68 87 755.5 0 87 69 150 755.5 0 150 70 161 755.5 0 161 71 1485 755.5 0 1485 72 6808 755.5 0 6808 73 1093 755.5 0 1093 74 15 755.5 0 15 75 35 755.5 0 35 76 12 755.5 0 12 77 124 755.5 0 124 78 102 755.5 0 102 79 63 755.5 0 63 80 71 755.5 0 71 81 132 755.5 0 132 82 75 755.5 0 75 83 60 755.5 0 60 84 187 755.5 0 187 85 72 755.5 0 72 86 33 755.5 0 33 87 65 755.5 0 65 88 46 755.5 0 46 89 30 755.5 0 30 90 19 755.5 0 19 91 102 755.5 0 102 92 96 755.5 0 96 93 269 755.5 0 269 94 185 755.5 0 185 95 139 755.5 0 139 96 659 755.5 0 659 97 339 755.5 0 339 98 769 755.5 0 769 99 36 755.5 0 36 100 16 755.5 0 16 101 17 755.5 0 17 102 11 755.5 0 11 103 7 755.5 0 7 104 17 755.5 0 17 105 14 755.5 0 14 106 16 755.5 0 16 107 11 755.5 0 11 108 34 755.5 0 34 109 40 755.5 0 40 110 14 755.5 0 14 111 29 755.5 0 29 112 30 755.5 0 30 113 23 755.5 0 23 114 34 755.5 0 34 115 57 755.5 0 57 116 72 755.5 0 72 117 83 755.5 0 83 118 47 755.5 0 47 119 44 755.5 0 44 120 39 755.5 0 39 121 46 755.5 0 46 122 47 755.5 0 47 123 98 755.5 0 98 124 90 755.5 0 90 125 114 755.5 0 114 126 607 755.5 0 607 127 62 755.5 0 62 128 60 755.5 0 60 129 107 755.5 0 107 130 136 755.5 0 136 131 78 755.5 0 78 132 173 755.5 0 173 133 53 755.5 0 53 134 89 755.5 0 89 135 136 755.5 0 136 136 94 755.5 0 94 137 80 755.5 0 80 138 60 755.5 0 60 139 50 755.5 0 50 140 34 755.5 0 34 141 41 755.5 0 41 142 32 755.5 0 32 143 74 755.5 0 74 144 85 755.5 0 85 145 166 755.5 0 166 146 86 755.5 0 86 147 34 755.5 0 34 148 36 755.5 0 36 149 21 755.5 0 21 150 49 755.5 0 49 151 40 755.5 0 40 152 60 755.5 0 60 153 61 755.5 0 61 154 96 755.5 0 96 155 211 755.5 0 211 156 125 755.5 0 125 157 91 755.5 0 91 158 62 755.5 0 62 159 42 755.5 0 42 160 50 755.5 0 50 161 44 755.5 0 44 162 46 755.5 0 46 163 80 755.5 0 80 164 96 755.5 0 96 165 132 755.5 0 132 166 94 755.5 0 94 167 117 755.5 0 117 168 96 755.5 0 96 169 43 755.5 0 43 170 41 755.5 0 41 171 70 755.5 0 70 172 132 755.5 0 132 173 111 755.5 0 111 174 92 755.5 0 92 175 54 755.5 0 54 176 48 755.5 0 48 177 54 755.5 0 54 178 101 755.5 0 101 179 110 755.5 0 110 180 113 755.5 0 113 181 74 755.5 0 74 182 72 755.5 0 72 183 73 755.5 0 73 184 60 755.5 0 60 185 65 755.5 0 65 186 51 755.5 0 51 187 24 755.5 0 24 188 44 755.5 0 44 189 57 755.5 0 57 190 210 755.5 0 210 191 227 755.5 0 227 192 784 755.5 0 784 193 168 755.5 0 168 194 162 755.5 0 162 195 46 755.5 0 46 196 93 755.5 0 93 197 48 755.5 0 48 198 43 755.5 0 43 199 47 755.5 0 47 200 74 755.5 0 74 201 59 755.5 0 59 202 91 755.5 0 91 203 176 755.5 0 176 204 195 755.5 0 195 205 77 755.5 0 77 206 37 755.5 0 37 207 41 755.5 0 41 208 53 755.5 0 53 209 75 755.5 0 75 210 164 755.5 0 164 211 713 755.5 0 713 212 147 755.5 0 147 213 68 755.5 0 68 214 66 755.5 0 66 215 90 755.5 0 90 216 203 755.5 0 203 217 113 755.5 0 113 218 91 755.5 0 91 219 48 755.5 0 48 220 149 755.5 0 149 221 414 755.5 0 414 222 355 755.5 0 355 223 90 755.5 0 90 224 96 755.5 0 96 225 114 755.5 0 114 226 226 755.5 0 226 227 116 755.5 0 116 228 62 755.5 0 62 229 32 755.5 0 32 230 29 755.5 0 29 231 16 755.5 0 16 232 75 755.5 0 75 233 123 755.5 0 123 234 70 755.5 0 70 235 69 755.5 0 69 236 126 755.5 0 126 237 75 755.5 0 75 238 65 755.5 0 65 239 40 755.5 0 40 240 40 755.5 0 40 241 88 755.5 0 88 242 141 755.5 0 141 243 105 755.5 0 105 244 77 755.5 0 77 245 85 755.5 0 85 246 68 755.5 0 68 247 111 755.5 0 111 248 23 755.5 0 23 249 55 755.5 0 55 250 84 755.5 0 84 251 281 755.5 0 281 252 74 755.5 0 74 253 67 755.5 0 67 254 67 755.5 0 67 255 106 755.5 0 106 256 97 755.5 0 97 257 58 755.5 0 58 258 73 755.5 0 73 259 53 755.5 0 53 260 49 755.5 0 49 261 46 755.5 0 46 262 69 755.5 0 69 263 71 755.5 0 71 264 163 755.5 0 163 265 157 755.5 0 157 266 152 755.5 0 152 267 44 755.5 0 44 268 44 755.5 0 44 269 37 755.5 0 37 270 76 755.5 0 76 271 274 755.5 0 274 272 203 755.5 0 203 273 60 755.5 0 60 274 66 755.5 0 66 275 91 755.5 0 91 276 57 755.5 0 57 277 100 755.5 0 100 278 209 755.5 0 209 279 316 755.5 0 316 280 205 755.5 0 205 281 33 755.5 0 33 282 30 755.5 0 30 283 44 755.5 0 44 284 46 755.5 0 46 285 136 755.5 0 136 286 183 755.5 0 183 287 185 755.5 0 185 288 79 755.5 0 79 289 66 755.5 0 66 290 148 755.5 0 148 291 157 755.5 0 157 292 90 755.5 0 90 293 45 755.5 0 45 294 54 755.5 0 54 295 100 755.5 0 100 296 395 755.5 0 395 297 921 755.5 0 921 298 188 755.5 0 188 299 91 755.5 0 91 300 158 755.5 0 158 301 133 755.5 0 133 Finished in 5.23 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 111,468 Reads with adapters: 87,703 (78.7%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 87,683 (78.7%) Total basepairs processed: 30,488,110 bp Total written (filtered): 24,286,272 bp (79.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 87703 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 20 0.0 3 0 0 18 2 16 549 0.0 3 0 515 17 17 17 86792 0.0 3 84243 2246 247 56 18 171 0.0 3 3 158 6 4 19 100 0.0 3 0 0 77 23 20 26 0.0 3 0 1 0 25 25 1 0.0 3 1 39 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 1 1 192 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 3 0.0 3 0 0 0 3 218 7 0.0 3 0 0 0 7 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.69 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 87,683 Reads with adapters: 5,981 (6.8%) Reads written (passing filters): 87,683 (100.0%) Total basepairs processed: 24,286,272 bp Total written (filtered): 24,263,841 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5981 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 10.6% G: 48.5% T: 24.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1540 1370.0 0 1540 4 4434 342.5 0 4434 5 4 85.6 0 4 6 1 21.4 0 1 15 1 0.0 1 0 1 34 1 0.0 2 0 0 1