This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.08 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 187,808 (6.1%) Reads that were too short: 7,629 (0.2%) Reads written (passing filters): 180,179 (5.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 51,183,298 bp (5.5%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 187808 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 951 755.5 0 951 7 2171 755.5 0 2171 8 2679 755.5 0 2679 9 159895 755.5 0 159895 10 129 755.5 0 129 11 13 755.5 0 13 12 9 755.5 0 9 13 12 755.5 0 12 14 8 755.5 0 8 15 10 755.5 0 10 16 43 755.5 0 43 17 39 755.5 0 39 18 192 755.5 0 192 19 10 755.5 0 10 20 35 755.5 0 35 21 10 755.5 0 10 22 28 755.5 0 28 23 10 755.5 0 10 24 13 755.5 0 13 25 11 755.5 0 11 26 8 755.5 0 8 27 11 755.5 0 11 28 11 755.5 0 11 29 10 755.5 0 10 30 9 755.5 0 9 31 12 755.5 0 12 32 17 755.5 0 17 33 17 755.5 0 17 34 10 755.5 0 10 35 15 755.5 0 15 36 11 755.5 0 11 37 20 755.5 0 20 38 11 755.5 0 11 39 5 755.5 0 5 40 8 755.5 0 8 41 14 755.5 0 14 42 11 755.5 0 11 43 18 755.5 0 18 44 10 755.5 0 10 45 12 755.5 0 12 46 32 755.5 0 32 47 78 755.5 0 78 48 609 755.5 0 609 49 7 755.5 0 7 50 18 755.5 0 18 51 8 755.5 0 8 52 15 755.5 0 15 53 14 755.5 0 14 54 15 755.5 0 15 55 9 755.5 0 9 56 72 755.5 0 72 57 41 755.5 0 41 58 61 755.5 0 61 59 12 755.5 0 12 60 9 755.5 0 9 61 11 755.5 0 11 62 12 755.5 0 12 63 13 755.5 0 13 64 14 755.5 0 14 65 11 755.5 0 11 66 5 755.5 0 5 67 8 755.5 0 8 68 4 755.5 0 4 69 15 755.5 0 15 70 13 755.5 0 13 71 14 755.5 0 14 72 7 755.5 0 7 73 12 755.5 0 12 74 15 755.5 0 15 75 11 755.5 0 11 76 16 755.5 0 16 77 61 755.5 0 61 78 67 755.5 0 67 79 197 755.5 0 197 80 105 755.5 0 105 81 541 755.5 0 541 82 179 755.5 0 179 83 71 755.5 0 71 84 132 755.5 0 132 85 143 755.5 0 143 86 110 755.5 0 110 87 59 755.5 0 59 88 46 755.5 0 46 89 57 755.5 0 57 90 38 755.5 0 38 91 50 755.5 0 50 92 68 755.5 0 68 93 108 755.5 0 108 94 837 755.5 0 837 95 1754 755.5 0 1754 96 2168 755.5 0 2168 97 122 755.5 0 122 98 148 755.5 0 148 99 25 755.5 0 25 100 24 755.5 0 24 101 28 755.5 0 28 102 22 755.5 0 22 103 15 755.5 0 15 104 10 755.5 0 10 105 8 755.5 0 8 106 14 755.5 0 14 107 9 755.5 0 9 108 10 755.5 0 10 109 16 755.5 0 16 110 206 755.5 0 206 111 22 755.5 0 22 112 48 755.5 0 48 113 34 755.5 0 34 114 12 755.5 0 12 115 29 755.5 0 29 116 37 755.5 0 37 117 41 755.5 0 41 118 66 755.5 0 66 119 52 755.5 0 52 120 38 755.5 0 38 121 24 755.5 0 24 122 28 755.5 0 28 123 22 755.5 0 22 124 36 755.5 0 36 125 44 755.5 0 44 126 54 755.5 0 54 127 33 755.5 0 33 128 59 755.5 0 59 129 25 755.5 0 25 130 45 755.5 0 45 131 79 755.5 0 79 132 142 755.5 0 142 133 100 755.5 0 100 134 43 755.5 0 43 135 34 755.5 0 34 136 58 755.5 0 58 137 129 755.5 0 129 138 114 755.5 0 114 139 105 755.5 0 105 140 39 755.5 0 39 141 66 755.5 0 66 142 34 755.5 0 34 143 56 755.5 0 56 144 44 755.5 0 44 145 203 755.5 0 203 146 226 755.5 0 226 147 49 755.5 0 49 148 28 755.5 0 28 149 28 755.5 0 28 150 30 755.5 0 30 151 34 755.5 0 34 152 19 755.5 0 19 153 22 755.5 0 22 154 31 755.5 0 31 155 22 755.5 0 22 156 38 755.5 0 38 157 36 755.5 0 36 158 62 755.5 0 62 159 46 755.5 0 46 160 60 755.5 0 60 161 33 755.5 0 33 162 32 755.5 0 32 163 40 755.5 0 40 164 35 755.5 0 35 165 46 755.5 0 46 166 53 755.5 0 53 167 49 755.5 0 49 168 50 755.5 0 50 169 30 755.5 0 30 170 74 755.5 0 74 171 23 755.5 0 23 172 27 755.5 0 27 173 22 755.5 0 22 174 46 755.5 0 46 175 30 755.5 0 30 176 32 755.5 0 32 177 20 755.5 0 20 178 27 755.5 0 27 179 59 755.5 0 59 180 67 755.5 0 67 181 72 755.5 0 72 182 52 755.5 0 52 183 38 755.5 0 38 184 61 755.5 0 61 185 192 755.5 0 192 186 98 755.5 0 98 187 93 755.5 0 93 188 58 755.5 0 58 189 18 755.5 0 18 190 34 755.5 0 34 191 53 755.5 0 53 192 130 755.5 0 130 193 80 755.5 0 80 194 296 755.5 0 296 195 54 755.5 0 54 196 16 755.5 0 16 197 19 755.5 0 19 198 58 755.5 0 58 199 48 755.5 0 48 200 39 755.5 0 39 201 12 755.5 0 12 202 19 755.5 0 19 203 42 755.5 0 42 204 101 755.5 0 101 205 280 755.5 0 280 206 116 755.5 0 116 207 76 755.5 0 76 208 23 755.5 0 23 209 29 755.5 0 29 210 32 755.5 0 32 211 89 755.5 0 89 212 65 755.5 0 65 213 71 755.5 0 71 214 61 755.5 0 61 215 44 755.5 0 44 216 44 755.5 0 44 217 60 755.5 0 60 218 79 755.5 0 79 219 59 755.5 0 59 220 51 755.5 0 51 221 17 755.5 0 17 222 22 755.5 0 22 223 31 755.5 0 31 224 50 755.5 0 50 225 37 755.5 0 37 226 48 755.5 0 48 227 61 755.5 0 61 228 80 755.5 0 80 229 68 755.5 0 68 230 31 755.5 0 31 231 38 755.5 0 38 232 29 755.5 0 29 233 25 755.5 0 25 234 51 755.5 0 51 235 77 755.5 0 77 236 70 755.5 0 70 237 53 755.5 0 53 238 79 755.5 0 79 239 132 755.5 0 132 240 214 755.5 0 214 241 32 755.5 0 32 242 26 755.5 0 26 243 37 755.5 0 37 244 39 755.5 0 39 245 66 755.5 0 66 246 40 755.5 0 40 247 42 755.5 0 42 248 22 755.5 0 22 249 40 755.5 0 40 250 23 755.5 0 23 251 17 755.5 0 17 252 25 755.5 0 25 253 55 755.5 0 55 254 73 755.5 0 73 255 33 755.5 0 33 256 24 755.5 0 24 257 40 755.5 0 40 258 20 755.5 0 20 259 44 755.5 0 44 260 27 755.5 0 27 261 74 755.5 0 74 262 174 755.5 0 174 263 137 755.5 0 137 264 33 755.5 0 33 265 45 755.5 0 45 266 73 755.5 0 73 267 72 755.5 0 72 268 75 755.5 0 75 269 40 755.5 0 40 270 18 755.5 0 18 271 100 755.5 0 100 272 266 755.5 0 266 273 79 755.5 0 79 274 88 755.5 0 88 275 48 755.5 0 48 276 42 755.5 0 42 277 29 755.5 0 29 278 58 755.5 0 58 279 114 755.5 0 114 280 64 755.5 0 64 281 35 755.5 0 35 282 19 755.5 0 19 283 35 755.5 0 35 284 32 755.5 0 32 285 44 755.5 0 44 286 71 755.5 0 71 287 89 755.5 0 89 288 122 755.5 0 122 289 197 755.5 0 197 290 241 755.5 0 241 291 131 755.5 0 131 292 143 755.5 0 143 293 58 755.5 0 58 294 60 755.5 0 60 295 58 755.5 0 58 296 69 755.5 0 69 297 262 755.5 0 262 298 417 755.5 0 417 299 457 755.5 0 457 300 45 755.5 0 45 301 136 755.5 0 136 Finished in 7.83 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 180,179 Reads with adapters: 165,069 (91.6%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 165,061 (91.6%) Total basepairs processed: 51,183,298 bp Total written (filtered): 45,398,111 bp (88.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 165069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 53 0.0 3 0 0 49 4 16 1270 0.0 3 0 1209 43 18 17 163250 0.0 3 158187 4490 437 136 18 300 0.0 3 8 263 13 16 19 81 0.0 3 1 0 64 16 20 72 0.0 3 43 1 1 27 22 1 0.0 3 1 24 1 0.0 3 1 27 1 0.0 3 1 28 1 0.0 3 1 29 1 0.0 3 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 1 38 1 0.0 3 1 44 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.16 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 165,061 Reads with adapters: 26,162 (15.8%) Reads written (passing filters): 165,061 (100.0%) Total basepairs processed: 45,398,111 bp Total written (filtered): 45,319,121 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 26162 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.2% C: 2.0% G: 41.6% T: 10.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 25759 2579.1 0 25759 4 390 644.8 0 390 6 1 40.3 0 1 7 1 10.1 0 1 10 1 0.2 1 0 1 13 10 0.0 1 0 10