This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.31 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 321,024 (10.4%) Reads that were too short: 8,058 (0.3%) Reads written (passing filters): 312,966 (10.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 59,142,206 bp (6.3%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 321024 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 184 755.5 0 184 7 2961 755.5 0 2961 8 194 755.5 0 194 9 8597 755.5 0 8597 10 38 755.5 0 38 11 12 755.5 0 12 12 11 755.5 0 11 13 10 755.5 0 10 14 90 755.5 0 90 15 21 755.5 0 21 16 27 755.5 0 27 17 10 755.5 0 10 18 13 755.5 0 13 19 16 755.5 0 16 20 8 755.5 0 8 21 10 755.5 0 10 22 22 755.5 0 22 23 17 755.5 0 17 24 61 755.5 0 61 25 11 755.5 0 11 26 16 755.5 0 16 27 17 755.5 0 17 28 17 755.5 0 17 29 13 755.5 0 13 30 22 755.5 0 22 31 53 755.5 0 53 32 41 755.5 0 41 33 44 755.5 0 44 34 98 755.5 0 98 35 47 755.5 0 47 36 28 755.5 0 28 37 64 755.5 0 64 38 78 755.5 0 78 39 25 755.5 0 25 40 20 755.5 0 20 41 43 755.5 0 43 42 10 755.5 0 10 43 24 755.5 0 24 44 7 755.5 0 7 45 19 755.5 0 19 46 17 755.5 0 17 47 13 755.5 0 13 48 45 755.5 0 45 49 8 755.5 0 8 50 20 755.5 0 20 51 9 755.5 0 9 52 25 755.5 0 25 53 19 755.5 0 19 54 36 755.5 0 36 55 17 755.5 0 17 56 82 755.5 0 82 57 45 755.5 0 45 58 243 755.5 0 243 59 80 755.5 0 80 60 24 755.5 0 24 61 29 755.5 0 29 62 11 755.5 0 11 63 19 755.5 0 19 64 16 755.5 0 16 65 15 755.5 0 15 66 122 755.5 0 122 67 9 755.5 0 9 68 26 755.5 0 26 69 9 755.5 0 9 70 19 755.5 0 19 71 18 755.5 0 18 72 10 755.5 0 10 73 11 755.5 0 11 74 19 755.5 0 19 75 15 755.5 0 15 76 34 755.5 0 34 77 357 755.5 0 357 78 70 755.5 0 70 79 22 755.5 0 22 80 25 755.5 0 25 81 32 755.5 0 32 82 16 755.5 0 16 83 91 755.5 0 91 84 86 755.5 0 86 85 48 755.5 0 48 86 52 755.5 0 52 87 232 755.5 0 232 88 27 755.5 0 27 89 26 755.5 0 26 90 29 755.5 0 29 91 45 755.5 0 45 92 46 755.5 0 46 93 45 755.5 0 45 94 75 755.5 0 75 95 39 755.5 0 39 96 124 755.5 0 124 97 448 755.5 0 448 98 74 755.5 0 74 99 121 755.5 0 121 100 105 755.5 0 105 101 69 755.5 0 69 102 147 755.5 0 147 103 139 755.5 0 139 104 160 755.5 0 160 105 57 755.5 0 57 106 115 755.5 0 115 107 352 755.5 0 352 108 703 755.5 0 703 109 573 755.5 0 573 110 940 755.5 0 940 111 800 755.5 0 800 112 3478 755.5 0 3478 113 8135 755.5 0 8135 114 230101 755.5 0 230101 115 6069 755.5 0 6069 116 22685 755.5 0 22685 117 152 755.5 0 152 118 47 755.5 0 47 119 28 755.5 0 28 120 37 755.5 0 37 121 18 755.5 0 18 122 29 755.5 0 29 123 33 755.5 0 33 124 34 755.5 0 34 125 15 755.5 0 15 126 43 755.5 0 43 127 22 755.5 0 22 128 47 755.5 0 47 129 194 755.5 0 194 130 60 755.5 0 60 131 62 755.5 0 62 132 29 755.5 0 29 133 14 755.5 0 14 134 22 755.5 0 22 135 40 755.5 0 40 136 121 755.5 0 121 137 84 755.5 0 84 138 64 755.5 0 64 139 39 755.5 0 39 140 119 755.5 0 119 141 336 755.5 0 336 142 833 755.5 0 833 143 473 755.5 0 473 144 55 755.5 0 55 145 113 755.5 0 113 146 276 755.5 0 276 147 4940 755.5 0 4940 148 837 755.5 0 837 149 1369 755.5 0 1369 150 130 755.5 0 130 151 132 755.5 0 132 152 248 755.5 0 248 153 4244 755.5 0 4244 154 382 755.5 0 382 155 3716 755.5 0 3716 156 233 755.5 0 233 157 76 755.5 0 76 158 170 755.5 0 170 159 34 755.5 0 34 160 31 755.5 0 31 161 40 755.5 0 40 162 62 755.5 0 62 163 43 755.5 0 43 164 53 755.5 0 53 165 46 755.5 0 46 166 67 755.5 0 67 167 72 755.5 0 72 168 384 755.5 0 384 169 123 755.5 0 123 170 77 755.5 0 77 171 30 755.5 0 30 172 18 755.5 0 18 173 19 755.5 0 19 174 19 755.5 0 19 175 24 755.5 0 24 176 114 755.5 0 114 177 11 755.5 0 11 178 21 755.5 0 21 179 18 755.5 0 18 180 17 755.5 0 17 181 76 755.5 0 76 182 66 755.5 0 66 183 57 755.5 0 57 184 76 755.5 0 76 185 88 755.5 0 88 186 103 755.5 0 103 187 30 755.5 0 30 188 26 755.5 0 26 189 10 755.5 0 10 190 79 755.5 0 79 191 98 755.5 0 98 192 28 755.5 0 28 193 33 755.5 0 33 194 15 755.5 0 15 195 12 755.5 0 12 196 11 755.5 0 11 197 12 755.5 0 12 198 26 755.5 0 26 199 25 755.5 0 25 200 15 755.5 0 15 201 19 755.5 0 19 202 270 755.5 0 270 203 33 755.5 0 33 204 87 755.5 0 87 205 52 755.5 0 52 206 26 755.5 0 26 207 19 755.5 0 19 208 15 755.5 0 15 209 20 755.5 0 20 210 29 755.5 0 29 211 19 755.5 0 19 212 33 755.5 0 33 213 42 755.5 0 42 214 34 755.5 0 34 215 47 755.5 0 47 216 117 755.5 0 117 217 102 755.5 0 102 218 75 755.5 0 75 219 29 755.5 0 29 220 12 755.5 0 12 221 24 755.5 0 24 222 16 755.5 0 16 223 12 755.5 0 12 224 39 755.5 0 39 225 61 755.5 0 61 226 106 755.5 0 106 227 60 755.5 0 60 228 106 755.5 0 106 229 40 755.5 0 40 230 23 755.5 0 23 231 14 755.5 0 14 232 24 755.5 0 24 233 24 755.5 0 24 234 30 755.5 0 30 235 41 755.5 0 41 236 51 755.5 0 51 237 18 755.5 0 18 238 26 755.5 0 26 239 11 755.5 0 11 240 4 755.5 0 4 241 6 755.5 0 6 242 15 755.5 0 15 243 12 755.5 0 12 244 36 755.5 0 36 245 50 755.5 0 50 246 35 755.5 0 35 247 22 755.5 0 22 248 16 755.5 0 16 249 22 755.5 0 22 250 29 755.5 0 29 251 104 755.5 0 104 252 30 755.5 0 30 253 53 755.5 0 53 254 80 755.5 0 80 255 122 755.5 0 122 256 54 755.5 0 54 257 39 755.5 0 39 258 21 755.5 0 21 259 8 755.5 0 8 260 6 755.5 0 6 261 10 755.5 0 10 262 11 755.5 0 11 263 7 755.5 0 7 264 22 755.5 0 22 265 29 755.5 0 29 266 35 755.5 0 35 267 49 755.5 0 49 268 53 755.5 0 53 269 37 755.5 0 37 270 37 755.5 0 37 271 66 755.5 0 66 272 44 755.5 0 44 273 27 755.5 0 27 274 18 755.5 0 18 275 118 755.5 0 118 276 45 755.5 0 45 277 33 755.5 0 33 278 36 755.5 0 36 279 128 755.5 0 128 280 105 755.5 0 105 281 288 755.5 0 288 282 313 755.5 0 313 283 1432 755.5 0 1432 284 15 755.5 0 15 285 13 755.5 0 13 286 11 755.5 0 11 287 38 755.5 0 38 288 20 755.5 0 20 289 763 755.5 0 763 290 582 755.5 0 582 291 381 755.5 0 381 292 171 755.5 0 171 293 173 755.5 0 173 294 112 755.5 0 112 295 82 755.5 0 82 296 134 755.5 0 134 297 21 755.5 0 21 298 14 755.5 0 14 299 19 755.5 0 19 300 7 755.5 0 7 301 8 755.5 0 8 Finished in 12.05 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 312,966 Reads with adapters: 3,176 (1.0%) Reads that were too short: 185 (0.1%) Reads written (passing filters): 2,991 (1.0%) Total basepairs processed: 59,142,206 bp Total written (filtered): 805,039 bp (1.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3176 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 12 0.0 3 0 9 1 2 17 2810 0.0 3 2721 81 7 1 18 17 0.0 3 1 12 2 2 19 4 0.0 3 0 0 1 3 20 10 0.0 3 0 0 0 10 32 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 51 7 0.0 3 0 0 0 7 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 85 102 0.0 3 0 0 0 102 88 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 5 0.0 3 0 0 1 4 135 1 0.0 3 0 0 0 1 136 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 44 0.0 3 0 0 1 43 175 113 0.0 3 0 0 0 113 176 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 5 0.0 3 0 0 0 5 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,991 Reads with adapters: 191 (6.4%) Reads written (passing filters): 2,991 (100.0%) Total basepairs processed: 805,039 bp Total written (filtered): 804,455 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 191 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.3% C: 57.1% G: 8.4% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 180 46.7 0 180 4 11 11.7 0 11