This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 94.05 s (30 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 73,512 (2.4%) Reads that were too short: 6,471 (0.2%) Reads written (passing filters): 67,041 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 15,452,538 bp (1.7%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 73512 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 323 755.5 0 323 7 2898 755.5 0 2898 8 10223 755.5 0 10223 9 46 755.5 0 46 10 5 755.5 0 5 11 124 755.5 0 124 12 34 755.5 0 34 13 24 755.5 0 24 14 75 755.5 0 75 15 11 755.5 0 11 16 9 755.5 0 9 17 14 755.5 0 14 18 22 755.5 0 22 19 19 755.5 0 19 20 23 755.5 0 23 21 19 755.5 0 19 22 14 755.5 0 14 23 21 755.5 0 21 24 16 755.5 0 16 25 12 755.5 0 12 26 26 755.5 0 26 27 23 755.5 0 23 28 16 755.5 0 16 29 63 755.5 0 63 30 19 755.5 0 19 31 15 755.5 0 15 32 17 755.5 0 17 33 16 755.5 0 16 34 12 755.5 0 12 35 12 755.5 0 12 36 27 755.5 0 27 37 20 755.5 0 20 38 43 755.5 0 43 39 140 755.5 0 140 40 32 755.5 0 32 41 50 755.5 0 50 42 30 755.5 0 30 43 39 755.5 0 39 44 82 755.5 0 82 45 57 755.5 0 57 46 58 755.5 0 58 47 29 755.5 0 29 48 49 755.5 0 49 49 107 755.5 0 107 50 123 755.5 0 123 51 202 755.5 0 202 52 216 755.5 0 216 53 247 755.5 0 247 54 548 755.5 0 548 55 944 755.5 0 944 56 19026 755.5 0 19026 57 4978 755.5 0 4978 58 9789 755.5 0 9789 59 60 755.5 0 60 60 18 755.5 0 18 61 44 755.5 0 44 62 747 755.5 0 747 63 38 755.5 0 38 64 23 755.5 0 23 65 7 755.5 0 7 66 14 755.5 0 14 67 26 755.5 0 26 68 11 755.5 0 11 69 9 755.5 0 9 70 23 755.5 0 23 71 14 755.5 0 14 72 56 755.5 0 56 73 21 755.5 0 21 74 13 755.5 0 13 75 18 755.5 0 18 76 11 755.5 0 11 77 12 755.5 0 12 78 21 755.5 0 21 79 44 755.5 0 44 80 24 755.5 0 24 81 25 755.5 0 25 82 30 755.5 0 30 83 127 755.5 0 127 84 68 755.5 0 68 85 101 755.5 0 101 86 43 755.5 0 43 87 67 755.5 0 67 88 79 755.5 0 79 89 286 755.5 0 286 90 100 755.5 0 100 91 20 755.5 0 20 92 12 755.5 0 12 93 47 755.5 0 47 94 60 755.5 0 60 95 23 755.5 0 23 96 19 755.5 0 19 97 20 755.5 0 20 98 8 755.5 0 8 99 16 755.5 0 16 100 16 755.5 0 16 101 25 755.5 0 25 102 18 755.5 0 18 103 33 755.5 0 33 104 42 755.5 0 42 105 148 755.5 0 148 106 12 755.5 0 12 107 16 755.5 0 16 108 9 755.5 0 9 109 13 755.5 0 13 110 15 755.5 0 15 111 29 755.5 0 29 112 22 755.5 0 22 113 24 755.5 0 24 114 23 755.5 0 23 115 15 755.5 0 15 116 23 755.5 0 23 117 35 755.5 0 35 118 17 755.5 0 17 119 194 755.5 0 194 120 56 755.5 0 56 121 55 755.5 0 55 122 31 755.5 0 31 123 27 755.5 0 27 124 26 755.5 0 26 125 46 755.5 0 46 126 130 755.5 0 130 127 26 755.5 0 26 128 101 755.5 0 101 129 15 755.5 0 15 130 28 755.5 0 28 131 26 755.5 0 26 132 32 755.5 0 32 133 36 755.5 0 36 134 26 755.5 0 26 135 34 755.5 0 34 136 29 755.5 0 29 137 24 755.5 0 24 138 43 755.5 0 43 139 46 755.5 0 46 140 46 755.5 0 46 141 104 755.5 0 104 142 179 755.5 0 179 143 30 755.5 0 30 144 30 755.5 0 30 145 70 755.5 0 70 146 96 755.5 0 96 147 680 755.5 0 680 148 246 755.5 0 246 149 87 755.5 0 87 150 173 755.5 0 173 151 51 755.5 0 51 152 183 755.5 0 183 153 44 755.5 0 44 154 41 755.5 0 41 155 27 755.5 0 27 156 41 755.5 0 41 157 54 755.5 0 54 158 47 755.5 0 47 159 48 755.5 0 48 160 112 755.5 0 112 161 41 755.5 0 41 162 65 755.5 0 65 163 59 755.5 0 59 164 50 755.5 0 50 165 82 755.5 0 82 166 138 755.5 0 138 167 1015 755.5 0 1015 168 470 755.5 0 470 169 364 755.5 0 364 170 77 755.5 0 77 171 146 755.5 0 146 172 222 755.5 0 222 173 236 755.5 0 236 174 121 755.5 0 121 175 105 755.5 0 105 176 83 755.5 0 83 177 231 755.5 0 231 178 227 755.5 0 227 179 51 755.5 0 51 180 52 755.5 0 52 181 83 755.5 0 83 182 68 755.5 0 68 183 44 755.5 0 44 184 48 755.5 0 48 185 48 755.5 0 48 186 66 755.5 0 66 187 22 755.5 0 22 188 37 755.5 0 37 189 27 755.5 0 27 190 55 755.5 0 55 191 72 755.5 0 72 192 110 755.5 0 110 193 88 755.5 0 88 194 109 755.5 0 109 195 138 755.5 0 138 196 257 755.5 0 257 197 491 755.5 0 491 198 121 755.5 0 121 199 307 755.5 0 307 200 3230 755.5 0 3230 201 438 755.5 0 438 202 849 755.5 0 849 203 42 755.5 0 42 204 36 755.5 0 36 205 42 755.5 0 42 206 26 755.5 0 26 207 49 755.5 0 49 208 18 755.5 0 18 209 15 755.5 0 15 210 11 755.5 0 11 211 22 755.5 0 22 212 21 755.5 0 21 213 36 755.5 0 36 214 28 755.5 0 28 215 34 755.5 0 34 216 46 755.5 0 46 217 50 755.5 0 50 218 38 755.5 0 38 219 90 755.5 0 90 220 31 755.5 0 31 221 72 755.5 0 72 222 29 755.5 0 29 223 62 755.5 0 62 224 85 755.5 0 85 225 52 755.5 0 52 226 36 755.5 0 36 227 48 755.5 0 48 228 53 755.5 0 53 229 121 755.5 0 121 230 46 755.5 0 46 231 80 755.5 0 80 232 280 755.5 0 280 233 59 755.5 0 59 234 71 755.5 0 71 235 22 755.5 0 22 236 13 755.5 0 13 237 13 755.5 0 13 238 12 755.5 0 12 239 12 755.5 0 12 240 18 755.5 0 18 241 31 755.5 0 31 242 47 755.5 0 47 243 35 755.5 0 35 244 56 755.5 0 56 245 50 755.5 0 50 246 20 755.5 0 20 247 9 755.5 0 9 248 25 755.5 0 25 249 42 755.5 0 42 250 26 755.5 0 26 251 49 755.5 0 49 252 136 755.5 0 136 253 126 755.5 0 126 254 85 755.5 0 85 255 76 755.5 0 76 256 32 755.5 0 32 257 18 755.5 0 18 258 13 755.5 0 13 259 13 755.5 0 13 260 10 755.5 0 10 261 45 755.5 0 45 262 26 755.5 0 26 263 50 755.5 0 50 264 64 755.5 0 64 265 101 755.5 0 101 266 81 755.5 0 81 267 18 755.5 0 18 268 22 755.5 0 22 269 30 755.5 0 30 270 30 755.5 0 30 271 36 755.5 0 36 272 42 755.5 0 42 273 74 755.5 0 74 274 23 755.5 0 23 275 33 755.5 0 33 276 37 755.5 0 37 277 51 755.5 0 51 278 86 755.5 0 86 279 157 755.5 0 157 280 285 755.5 0 285 281 25 755.5 0 25 282 37 755.5 0 37 283 81 755.5 0 81 284 35 755.5 0 35 285 14 755.5 0 14 286 23 755.5 0 23 287 22 755.5 0 22 288 20 755.5 0 20 289 54 755.5 0 54 290 42 755.5 0 42 291 120 755.5 0 120 292 409 755.5 0 409 293 352 755.5 0 352 294 90 755.5 0 90 295 46 755.5 0 46 296 18 755.5 0 18 297 19 755.5 0 19 298 46 755.5 0 46 299 113 755.5 0 113 300 38 755.5 0 38 301 9 755.5 0 9 Finished in 2.93 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 67,041 Reads with adapters: 2,720 (4.1%) Reads that were too short: 67 (0.1%) Reads written (passing filters): 2,653 (4.0%) Total basepairs processed: 15,452,538 bp Total written (filtered): 728,968 bp (4.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2720 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 9 0.0 3 0 8 0 1 17 2589 0.0 3 2545 41 3 18 8 0.0 3 1 4 3 19 2 0.0 3 0 0 1 1 20 13 0.0 3 2 0 0 11 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 5 0.0 3 0 0 0 5 152 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 196 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 231 6 0.0 3 0 0 0 6 232 22 0.0 3 0 0 0 22 233 5 0.0 3 0 0 0 5 234 1 0.0 3 0 0 1 241 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 2,653 Reads with adapters: 229 (8.6%) Reads written (passing filters): 2,653 (100.0%) Total basepairs processed: 728,968 bp Total written (filtered): 728,235 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 229 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.4% C: 22.7% G: 12.7% T: 12.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 188 41.5 0 188 4 37 10.4 0 37 5 3 2.6 0 3 6 1 0.6 0 1