This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.10 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 128,940 (4.2%) Reads that were too short: 22,788 (0.7%) Reads written (passing filters): 106,152 (3.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 27,137,167 bp (2.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 128940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 962 755.5 0 962 7 565 755.5 0 565 8 617 755.5 0 617 9 47026 755.5 0 47026 10 55 755.5 0 55 11 5 755.5 0 5 12 15 755.5 0 15 13 9 755.5 0 9 14 13 755.5 0 13 15 9 755.5 0 9 16 16 755.5 0 16 17 6 755.5 0 6 18 15 755.5 0 15 19 8 755.5 0 8 20 10 755.5 0 10 21 11 755.5 0 11 22 13 755.5 0 13 23 14 755.5 0 14 24 16 755.5 0 16 25 20 755.5 0 20 26 21 755.5 0 21 27 30 755.5 0 30 28 54 755.5 0 54 29 137 755.5 0 137 30 41 755.5 0 41 31 52 755.5 0 52 32 76 755.5 0 76 33 41 755.5 0 41 34 57 755.5 0 57 35 81 755.5 0 81 36 69 755.5 0 69 37 73 755.5 0 73 38 120 755.5 0 120 39 92 755.5 0 92 40 97 755.5 0 97 41 132 755.5 0 132 42 1658 755.5 0 1658 43 3638 755.5 0 3638 44 7185 755.5 0 7185 45 43 755.5 0 43 46 16 755.5 0 16 47 39 755.5 0 39 48 77 755.5 0 77 49 35 755.5 0 35 50 22 755.5 0 22 51 24 755.5 0 24 52 27 755.5 0 27 53 55 755.5 0 55 54 54 755.5 0 54 55 45 755.5 0 45 56 30 755.5 0 30 57 36 755.5 0 36 58 76 755.5 0 76 59 88 755.5 0 88 60 140 755.5 0 140 61 216 755.5 0 216 62 206 755.5 0 206 63 431 755.5 0 431 64 685 755.5 0 685 65 13321 755.5 0 13321 66 5290 755.5 0 5290 67 9959 755.5 0 9959 68 58 755.5 0 58 69 10 755.5 0 10 70 11 755.5 0 11 71 31 755.5 0 31 72 9 755.5 0 9 73 11 755.5 0 11 74 11 755.5 0 11 75 42 755.5 0 42 76 35 755.5 0 35 77 21 755.5 0 21 78 29 755.5 0 29 79 37 755.5 0 37 80 55 755.5 0 55 81 96 755.5 0 96 82 52 755.5 0 52 83 21 755.5 0 21 84 67 755.5 0 67 85 74 755.5 0 74 86 63 755.5 0 63 87 249 755.5 0 249 88 90 755.5 0 90 89 40 755.5 0 40 90 118 755.5 0 118 91 74 755.5 0 74 92 42 755.5 0 42 93 34 755.5 0 34 94 74 755.5 0 74 95 56 755.5 0 56 96 166 755.5 0 166 97 196 755.5 0 196 98 50 755.5 0 50 99 26 755.5 0 26 100 41 755.5 0 41 101 81 755.5 0 81 102 133 755.5 0 133 103 84 755.5 0 84 104 46 755.5 0 46 105 15 755.5 0 15 106 21 755.5 0 21 107 44 755.5 0 44 108 64 755.5 0 64 109 9 755.5 0 9 110 18 755.5 0 18 111 32 755.5 0 32 112 116 755.5 0 116 113 60 755.5 0 60 114 51 755.5 0 51 115 65 755.5 0 65 116 53 755.5 0 53 117 44 755.5 0 44 118 50 755.5 0 50 119 118 755.5 0 118 120 104 755.5 0 104 121 71 755.5 0 71 122 96 755.5 0 96 123 48 755.5 0 48 124 66 755.5 0 66 125 79 755.5 0 79 126 66 755.5 0 66 127 80 755.5 0 80 128 125 755.5 0 125 129 80 755.5 0 80 130 44 755.5 0 44 131 51 755.5 0 51 132 71 755.5 0 71 133 229 755.5 0 229 134 246 755.5 0 246 135 111 755.5 0 111 136 128 755.5 0 128 137 172 755.5 0 172 138 349 755.5 0 349 139 172 755.5 0 172 140 100 755.5 0 100 141 133 755.5 0 133 142 99 755.5 0 99 143 82 755.5 0 82 144 58 755.5 0 58 145 53 755.5 0 53 146 63 755.5 0 63 147 91 755.5 0 91 148 87 755.5 0 87 149 119 755.5 0 119 150 103 755.5 0 103 151 70 755.5 0 70 152 34 755.5 0 34 153 52 755.5 0 52 154 78 755.5 0 78 155 108 755.5 0 108 156 80 755.5 0 80 157 62 755.5 0 62 158 97 755.5 0 97 159 250 755.5 0 250 160 164 755.5 0 164 161 101 755.5 0 101 162 85 755.5 0 85 163 115 755.5 0 115 164 139 755.5 0 139 165 122 755.5 0 122 166 106 755.5 0 106 167 90 755.5 0 90 168 99 755.5 0 99 169 69 755.5 0 69 170 146 755.5 0 146 171 159 755.5 0 159 172 113 755.5 0 113 173 70 755.5 0 70 174 96 755.5 0 96 175 118 755.5 0 118 176 119 755.5 0 119 177 67 755.5 0 67 178 55 755.5 0 55 179 52 755.5 0 52 180 73 755.5 0 73 181 164 755.5 0 164 182 156 755.5 0 156 183 126 755.5 0 126 184 155 755.5 0 155 185 156 755.5 0 156 186 201 755.5 0 201 187 180 755.5 0 180 188 159 755.5 0 159 189 104 755.5 0 104 190 65 755.5 0 65 191 58 755.5 0 58 192 71 755.5 0 71 193 64 755.5 0 64 194 140 755.5 0 140 195 160 755.5 0 160 196 91 755.5 0 91 197 45 755.5 0 45 198 44 755.5 0 44 199 102 755.5 0 102 200 314 755.5 0 314 201 165 755.5 0 165 202 48 755.5 0 48 203 53 755.5 0 53 204 88 755.5 0 88 205 87 755.5 0 87 206 195 755.5 0 195 207 273 755.5 0 273 208 186 755.5 0 186 209 103 755.5 0 103 210 100 755.5 0 100 211 136 755.5 0 136 212 96 755.5 0 96 213 116 755.5 0 116 214 115 755.5 0 115 215 148 755.5 0 148 216 183 755.5 0 183 217 210 755.5 0 210 218 175 755.5 0 175 219 246 755.5 0 246 220 278 755.5 0 278 221 146 755.5 0 146 222 55 755.5 0 55 223 73 755.5 0 73 224 327 755.5 0 327 225 251 755.5 0 251 226 140 755.5 0 140 227 141 755.5 0 141 228 199 755.5 0 199 229 153 755.5 0 153 230 222 755.5 0 222 231 255 755.5 0 255 232 99 755.5 0 99 233 49 755.5 0 49 234 38 755.5 0 38 235 184 755.5 0 184 236 242 755.5 0 242 237 479 755.5 0 479 238 124 755.5 0 124 239 95 755.5 0 95 240 126 755.5 0 126 241 107 755.5 0 107 242 66 755.5 0 66 243 89 755.5 0 89 244 202 755.5 0 202 245 208 755.5 0 208 246 214 755.5 0 214 247 156 755.5 0 156 248 184 755.5 0 184 249 225 755.5 0 225 250 132 755.5 0 132 251 72 755.5 0 72 252 51 755.5 0 51 253 74 755.5 0 74 254 298 755.5 0 298 255 2579 755.5 0 2579 256 1549 755.5 0 1549 257 674 755.5 0 674 258 432 755.5 0 432 259 234 755.5 0 234 260 156 755.5 0 156 261 92 755.5 0 92 262 111 755.5 0 111 263 56 755.5 0 56 264 92 755.5 0 92 265 75 755.5 0 75 266 104 755.5 0 104 267 217 755.5 0 217 268 358 755.5 0 358 269 268 755.5 0 268 270 161 755.5 0 161 271 161 755.5 0 161 272 143 755.5 0 143 273 93 755.5 0 93 274 947 755.5 0 947 275 733 755.5 0 733 276 313 755.5 0 313 277 50 755.5 0 50 278 68 755.5 0 68 279 88 755.5 0 88 280 173 755.5 0 173 281 202 755.5 0 202 282 140 755.5 0 140 283 86 755.5 0 86 284 73 755.5 0 73 285 123 755.5 0 123 286 136 755.5 0 136 287 156 755.5 0 156 288 130 755.5 0 130 289 193 755.5 0 193 290 237 755.5 0 237 291 265 755.5 0 265 292 109 755.5 0 109 293 271 755.5 0 271 294 410 755.5 0 410 295 283 755.5 0 283 296 167 755.5 0 167 297 206 755.5 0 206 298 203 755.5 0 203 299 166 755.5 0 166 300 192 755.5 0 192 301 801 755.5 0 801 Finished in 4.75 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 106,152 Reads with adapters: 48,171 (45.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 48,162 (45.4%) Total basepairs processed: 27,137,167 bp Total written (filtered): 13,244,357 bp (48.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 15 0.0 3 0 0 13 2 16 479 0.0 3 0 443 23 13 17 47406 0.0 3 45835 1369 155 47 18 131 0.0 3 12 107 7 5 19 31 0.0 3 0 0 20 11 20 78 0.0 3 0 0 0 78 23 1 0.0 3 0 0 1 24 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.01 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 48,162 Reads with adapters: 3,680 (7.6%) Reads written (passing filters): 48,162 (100.0%) Total basepairs processed: 13,244,357 bp Total written (filtered): 13,232,837 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3680 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.8% C: 5.2% G: 13.7% T: 11.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3202 752.5 0 3202 4 476 188.1 0 476 5 2 47.0 0 2