This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.26 s (29 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 83,323 (2.7%) Reads that were too short: 8,493 (0.3%) Reads written (passing filters): 74,830 (2.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 19,774,286 bp (2.1%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 83323 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1767 755.5 0 1767 7 49176 755.5 0 49176 8 54 755.5 0 54 9 41 755.5 0 41 10 15 755.5 0 15 11 11 755.5 0 11 12 12 755.5 0 12 13 8 755.5 0 8 14 11 755.5 0 11 15 5 755.5 0 5 16 3 755.5 0 3 17 17 755.5 0 17 18 11 755.5 0 11 19 4 755.5 0 4 20 5 755.5 0 5 21 4 755.5 0 4 22 9 755.5 0 9 23 8 755.5 0 8 24 10 755.5 0 10 25 5 755.5 0 5 26 14 755.5 0 14 27 31 755.5 0 31 28 22 755.5 0 22 29 15 755.5 0 15 30 19 755.5 0 19 31 10 755.5 0 10 32 11 755.5 0 11 33 9 755.5 0 9 34 12 755.5 0 12 35 97 755.5 0 97 36 12 755.5 0 12 37 24 755.5 0 24 38 11 755.5 0 11 39 10 755.5 0 10 40 21 755.5 0 21 41 91 755.5 0 91 42 30 755.5 0 30 43 15 755.5 0 15 44 14 755.5 0 14 45 15 755.5 0 15 46 22 755.5 0 22 47 16 755.5 0 16 48 23 755.5 0 23 49 40 755.5 0 40 50 39 755.5 0 39 51 59 755.5 0 59 52 116 755.5 0 116 53 328 755.5 0 328 54 4654 755.5 0 4654 55 1724 755.5 0 1724 56 1009 755.5 0 1009 57 8 755.5 0 8 58 7 755.5 0 7 59 6 755.5 0 6 60 9 755.5 0 9 61 7 755.5 0 7 62 4 755.5 0 4 63 10 755.5 0 10 64 4 755.5 0 4 65 4 755.5 0 4 66 6 755.5 0 6 67 1 755.5 0 1 68 4 755.5 0 4 69 8 755.5 0 8 70 9 755.5 0 9 71 3 755.5 0 3 72 5 755.5 0 5 73 5 755.5 0 5 74 4 755.5 0 4 75 3 755.5 0 3 76 8 755.5 0 8 77 10 755.5 0 10 78 5 755.5 0 5 79 17 755.5 0 17 80 25 755.5 0 25 81 70 755.5 0 70 82 17 755.5 0 17 83 38 755.5 0 38 84 66 755.5 0 66 85 28 755.5 0 28 86 17 755.5 0 17 87 43 755.5 0 43 88 123 755.5 0 123 89 42 755.5 0 42 90 32 755.5 0 32 91 78 755.5 0 78 92 149 755.5 0 149 93 94 755.5 0 94 94 1149 755.5 0 1149 95 870 755.5 0 870 96 4177 755.5 0 4177 97 47 755.5 0 47 98 60 755.5 0 60 99 21 755.5 0 21 100 10 755.5 0 10 101 15 755.5 0 15 102 34 755.5 0 34 103 14 755.5 0 14 104 25 755.5 0 25 105 11 755.5 0 11 106 3 755.5 0 3 107 10 755.5 0 10 108 41 755.5 0 41 109 42 755.5 0 42 110 17 755.5 0 17 111 52 755.5 0 52 112 6 755.5 0 6 113 19 755.5 0 19 114 20 755.5 0 20 115 8 755.5 0 8 116 37 755.5 0 37 117 60 755.5 0 60 118 31 755.5 0 31 119 79 755.5 0 79 120 84 755.5 0 84 121 230 755.5 0 230 122 120 755.5 0 120 123 431 755.5 0 431 124 34 755.5 0 34 125 23 755.5 0 23 126 21 755.5 0 21 127 27 755.5 0 27 128 38 755.5 0 38 129 72 755.5 0 72 130 37 755.5 0 37 131 32 755.5 0 32 132 27 755.5 0 27 133 40 755.5 0 40 134 35 755.5 0 35 135 34 755.5 0 34 136 49 755.5 0 49 137 52 755.5 0 52 138 53 755.5 0 53 139 105 755.5 0 105 140 57 755.5 0 57 141 135 755.5 0 135 142 48 755.5 0 48 143 66 755.5 0 66 144 28 755.5 0 28 145 32 755.5 0 32 146 33 755.5 0 33 147 83 755.5 0 83 148 53 755.5 0 53 149 32 755.5 0 32 150 31 755.5 0 31 151 35 755.5 0 35 152 49 755.5 0 49 153 30 755.5 0 30 154 33 755.5 0 33 155 43 755.5 0 43 156 43 755.5 0 43 157 45 755.5 0 45 158 62 755.5 0 62 159 48 755.5 0 48 160 107 755.5 0 107 161 56 755.5 0 56 162 100 755.5 0 100 163 80 755.5 0 80 164 43 755.5 0 43 165 72 755.5 0 72 166 44 755.5 0 44 167 140 755.5 0 140 168 101 755.5 0 101 169 46 755.5 0 46 170 33 755.5 0 33 171 64 755.5 0 64 172 114 755.5 0 114 173 42 755.5 0 42 174 35 755.5 0 35 175 88 755.5 0 88 176 1393 755.5 0 1393 177 418 755.5 0 418 178 580 755.5 0 580 179 27 755.5 0 27 180 29 755.5 0 29 181 72 755.5 0 72 182 67 755.5 0 67 183 76 755.5 0 76 184 64 755.5 0 64 185 35 755.5 0 35 186 33 755.5 0 33 187 44 755.5 0 44 188 141 755.5 0 141 189 65 755.5 0 65 190 26 755.5 0 26 191 27 755.5 0 27 192 89 755.5 0 89 193 52 755.5 0 52 194 59 755.5 0 59 195 81 755.5 0 81 196 90 755.5 0 90 197 27 755.5 0 27 198 10 755.5 0 10 199 16 755.5 0 16 200 37 755.5 0 37 201 61 755.5 0 61 202 45 755.5 0 45 203 38 755.5 0 38 204 36 755.5 0 36 205 84 755.5 0 84 206 52 755.5 0 52 207 47 755.5 0 47 208 78 755.5 0 78 209 51 755.5 0 51 210 41 755.5 0 41 211 43 755.5 0 43 212 50 755.5 0 50 213 76 755.5 0 76 214 57 755.5 0 57 215 60 755.5 0 60 216 73 755.5 0 73 217 54 755.5 0 54 218 43 755.5 0 43 219 50 755.5 0 50 220 99 755.5 0 99 221 220 755.5 0 220 222 98 755.5 0 98 223 31 755.5 0 31 224 47 755.5 0 47 225 60 755.5 0 60 226 65 755.5 0 65 227 50 755.5 0 50 228 70 755.5 0 70 229 54 755.5 0 54 230 54 755.5 0 54 231 43 755.5 0 43 232 138 755.5 0 138 233 115 755.5 0 115 234 44 755.5 0 44 235 22 755.5 0 22 236 46 755.5 0 46 237 81 755.5 0 81 238 51 755.5 0 51 239 44 755.5 0 44 240 52 755.5 0 52 241 55 755.5 0 55 242 71 755.5 0 71 243 34 755.5 0 34 244 32 755.5 0 32 245 28 755.5 0 28 246 35 755.5 0 35 247 44 755.5 0 44 248 152 755.5 0 152 249 242 755.5 0 242 250 112 755.5 0 112 251 53 755.5 0 53 252 43 755.5 0 43 253 66 755.5 0 66 254 79 755.5 0 79 255 65 755.5 0 65 256 56 755.5 0 56 257 53 755.5 0 53 258 76 755.5 0 76 259 80 755.5 0 80 260 97 755.5 0 97 261 117 755.5 0 117 262 293 755.5 0 293 263 142 755.5 0 142 264 120 755.5 0 120 265 57 755.5 0 57 266 44 755.5 0 44 267 38 755.5 0 38 268 63 755.5 0 63 269 100 755.5 0 100 270 95 755.5 0 95 271 64 755.5 0 64 272 48 755.5 0 48 273 78 755.5 0 78 274 63 755.5 0 63 275 46 755.5 0 46 276 87 755.5 0 87 277 63 755.5 0 63 278 80 755.5 0 80 279 68 755.5 0 68 280 193 755.5 0 193 281 119 755.5 0 119 282 325 755.5 0 325 283 121 755.5 0 121 284 71 755.5 0 71 285 44 755.5 0 44 286 39 755.5 0 39 287 43 755.5 0 43 288 105 755.5 0 105 289 983 755.5 0 983 290 218 755.5 0 218 291 122 755.5 0 122 292 72 755.5 0 72 293 38 755.5 0 38 294 54 755.5 0 54 295 64 755.5 0 64 296 55 755.5 0 55 297 42 755.5 0 42 298 82 755.5 0 82 299 44 755.5 0 44 300 39 755.5 0 39 301 19 755.5 0 19 Finished in 3.50 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 74,830 Reads with adapters: 50,904 (68.0%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 50,901 (68.0%) Total basepairs processed: 19,774,286 bp Total written (filtered): 13,948,873 bp (70.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 50904 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 19 0.0 3 0 0 19 19 524 0.0 3 0 502 18 2 2 20 50196 0.0 4 49365 801 21 5 4 21 106 0.0 4 11 90 2 3 22 14 0.0 4 1 0 13 23 36 0.0 4 2 0 0 32 2 25 1 0.0 4 1 30 1 0.0 4 1 31 1 0.0 4 0 0 1 39 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.94 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 50,901 Reads with adapters: 390 (0.8%) Reads written (passing filters): 50,901 (100.0%) Total basepairs processed: 13,948,873 bp Total written (filtered): 13,942,619 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 390 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 1.0% G: 92.3% T: 3.1% none/other: 0.3% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 795.3 0 12 4 2 198.8 0 2 5 2 49.7 0 2 10 1 0.0 1 0 1 11 351 0.0 1 2 349 12 11 0.0 1 0 11 13 1 0.0 1 1 15 2 0.0 1 0 2 265 1 0.0 1 1 269 5 0.0 1 5 270 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.