This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.34 s (30 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 106,167 (3.4%) Reads that were too short: 29,942 (1.0%) Reads written (passing filters): 76,225 (2.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 21,011,074 bp (2.3%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 106167 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 615 755.5 0 615 7 784 755.5 0 784 8 53452 755.5 0 53452 9 9117 755.5 0 9117 10 17 755.5 0 17 11 9 755.5 0 9 12 9 755.5 0 9 13 11 755.5 0 11 14 6 755.5 0 6 15 6 755.5 0 6 16 12 755.5 0 12 17 20 755.5 0 20 18 17 755.5 0 17 19 9 755.5 0 9 20 10 755.5 0 10 21 7 755.5 0 7 22 129 755.5 0 129 23 16 755.5 0 16 24 41 755.5 0 41 25 16 755.5 0 16 26 24 755.5 0 24 27 42 755.5 0 42 28 16 755.5 0 16 29 13 755.5 0 13 30 13 755.5 0 13 31 225 755.5 0 225 32 7 755.5 0 7 33 8 755.5 0 8 34 13 755.5 0 13 35 19 755.5 0 19 36 38 755.5 0 38 37 17 755.5 0 17 38 5 755.5 0 5 39 9 755.5 0 9 40 8 755.5 0 8 41 9 755.5 0 9 42 16 755.5 0 16 43 15 755.5 0 15 44 9 755.5 0 9 45 8 755.5 0 8 46 12 755.5 0 12 47 15 755.5 0 15 48 6 755.5 0 6 49 4 755.5 0 4 50 8 755.5 0 8 51 9 755.5 0 9 52 19 755.5 0 19 53 11 755.5 0 11 54 45 755.5 0 45 55 11 755.5 0 11 56 5 755.5 0 5 57 10 755.5 0 10 58 14 755.5 0 14 59 12 755.5 0 12 60 9 755.5 0 9 61 12 755.5 0 12 62 6 755.5 0 6 63 19 755.5 0 19 64 16 755.5 0 16 65 15 755.5 0 15 66 13 755.5 0 13 67 25 755.5 0 25 68 38 755.5 0 38 69 938 755.5 0 938 70 23 755.5 0 23 71 114 755.5 0 114 72 18 755.5 0 18 73 9 755.5 0 9 74 14 755.5 0 14 75 20 755.5 0 20 76 92 755.5 0 92 77 20 755.5 0 20 78 79 755.5 0 79 79 47 755.5 0 47 80 428 755.5 0 428 81 78 755.5 0 78 82 148 755.5 0 148 83 49 755.5 0 49 84 57 755.5 0 57 85 23 755.5 0 23 86 25 755.5 0 25 87 29 755.5 0 29 88 36 755.5 0 36 89 19 755.5 0 19 90 15 755.5 0 15 91 34 755.5 0 34 92 38 755.5 0 38 93 38 755.5 0 38 94 55 755.5 0 55 95 44 755.5 0 44 96 365 755.5 0 365 97 549 755.5 0 549 98 1840 755.5 0 1840 99 50 755.5 0 50 100 22 755.5 0 22 101 15 755.5 0 15 102 22 755.5 0 22 103 11 755.5 0 11 104 17 755.5 0 17 105 18 755.5 0 18 106 16 755.5 0 16 107 15 755.5 0 15 108 37 755.5 0 37 109 26 755.5 0 26 110 23 755.5 0 23 111 17 755.5 0 17 112 349 755.5 0 349 113 44 755.5 0 44 114 127 755.5 0 127 115 40 755.5 0 40 116 51 755.5 0 51 117 21 755.5 0 21 118 29 755.5 0 29 119 28 755.5 0 28 120 69 755.5 0 69 121 28 755.5 0 28 122 31 755.5 0 31 123 27 755.5 0 27 124 22 755.5 0 22 125 17 755.5 0 17 126 29 755.5 0 29 127 33 755.5 0 33 128 55 755.5 0 55 129 61 755.5 0 61 130 89 755.5 0 89 131 32 755.5 0 32 132 177 755.5 0 177 133 98 755.5 0 98 134 210 755.5 0 210 135 48 755.5 0 48 136 34 755.5 0 34 137 37 755.5 0 37 138 36 755.5 0 36 139 78 755.5 0 78 140 20 755.5 0 20 141 32 755.5 0 32 142 39 755.5 0 39 143 25 755.5 0 25 144 28 755.5 0 28 145 21 755.5 0 21 146 26 755.5 0 26 147 23 755.5 0 23 148 37 755.5 0 37 149 46 755.5 0 46 150 90 755.5 0 90 151 22 755.5 0 22 152 22 755.5 0 22 153 23 755.5 0 23 154 33 755.5 0 33 155 46 755.5 0 46 156 60 755.5 0 60 157 27 755.5 0 27 158 40 755.5 0 40 159 50 755.5 0 50 160 55 755.5 0 55 161 51 755.5 0 51 162 46 755.5 0 46 163 36 755.5 0 36 164 45 755.5 0 45 165 37 755.5 0 37 166 35 755.5 0 35 167 49 755.5 0 49 168 69 755.5 0 69 169 29 755.5 0 29 170 31 755.5 0 31 171 38 755.5 0 38 172 53 755.5 0 53 173 28 755.5 0 28 174 24 755.5 0 24 175 34 755.5 0 34 176 29 755.5 0 29 177 37 755.5 0 37 178 48 755.5 0 48 179 29 755.5 0 29 180 31 755.5 0 31 181 47 755.5 0 47 182 74 755.5 0 74 183 98 755.5 0 98 184 151 755.5 0 151 185 140 755.5 0 140 186 182 755.5 0 182 187 31 755.5 0 31 188 280 755.5 0 280 189 304 755.5 0 304 190 325 755.5 0 325 191 67 755.5 0 67 192 68 755.5 0 68 193 33 755.5 0 33 194 24 755.5 0 24 195 32 755.5 0 32 196 90 755.5 0 90 197 39 755.5 0 39 198 19 755.5 0 19 199 21 755.5 0 21 200 22 755.5 0 22 201 50 755.5 0 50 202 56 755.5 0 56 203 41 755.5 0 41 204 42 755.5 0 42 205 44 755.5 0 44 206 52 755.5 0 52 207 64 755.5 0 64 208 96 755.5 0 96 209 224 755.5 0 224 210 263 755.5 0 263 211 780 755.5 0 780 212 105 755.5 0 105 213 83 755.5 0 83 214 85 755.5 0 85 215 78 755.5 0 78 216 71 755.5 0 71 217 61 755.5 0 61 218 38 755.5 0 38 219 28 755.5 0 28 220 42 755.5 0 42 221 60 755.5 0 60 222 96 755.5 0 96 223 74 755.5 0 74 224 61 755.5 0 61 225 130 755.5 0 130 226 102 755.5 0 102 227 65 755.5 0 65 228 175 755.5 0 175 229 64 755.5 0 64 230 70 755.5 0 70 231 54 755.5 0 54 232 85 755.5 0 85 233 116 755.5 0 116 234 141 755.5 0 141 235 135 755.5 0 135 236 287 755.5 0 287 237 148 755.5 0 148 238 120 755.5 0 120 239 100 755.5 0 100 240 382 755.5 0 382 241 1055 755.5 0 1055 242 7888 755.5 0 7888 243 1129 755.5 0 1129 244 101 755.5 0 101 245 105 755.5 0 105 246 451 755.5 0 451 247 154 755.5 0 154 248 911 755.5 0 911 249 505 755.5 0 505 250 707 755.5 0 707 251 6259 755.5 0 6259 252 119 755.5 0 119 253 102 755.5 0 102 254 190 755.5 0 190 255 387 755.5 0 387 256 86 755.5 0 86 257 44 755.5 0 44 258 37 755.5 0 37 259 45 755.5 0 45 260 61 755.5 0 61 261 180 755.5 0 180 262 474 755.5 0 474 263 70 755.5 0 70 264 63 755.5 0 63 265 43 755.5 0 43 266 68 755.5 0 68 267 67 755.5 0 67 268 66 755.5 0 66 269 69 755.5 0 69 270 193 755.5 0 193 271 923 755.5 0 923 272 177 755.5 0 177 273 101 755.5 0 101 274 292 755.5 0 292 275 93 755.5 0 93 276 48 755.5 0 48 277 59 755.5 0 59 278 41 755.5 0 41 279 42 755.5 0 42 280 32 755.5 0 32 281 49 755.5 0 49 282 40 755.5 0 40 283 48 755.5 0 48 284 40 755.5 0 40 285 45 755.5 0 45 286 22 755.5 0 22 287 30 755.5 0 30 288 45 755.5 0 45 289 114 755.5 0 114 290 161 755.5 0 161 291 78 755.5 0 78 292 50 755.5 0 50 293 50 755.5 0 50 294 56 755.5 0 56 295 52 755.5 0 52 296 74 755.5 0 74 297 83 755.5 0 83 298 97 755.5 0 97 299 103 755.5 0 103 300 91 755.5 0 91 301 459 755.5 0 459 Finished in 3.45 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 76,225 Reads with adapters: 54,302 (71.2%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 54,292 (71.2%) Total basepairs processed: 21,011,074 bp Total written (filtered): 14,981,902 bp (71.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 54302 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 25 0.0 3 0 0 16 9 16 638 0.0 3 0 586 33 19 17 52841 0.0 3 51101 1516 185 39 18 136 0.0 3 5 108 16 7 19 246 0.0 3 5 1 213 27 20 373 0.0 3 230 6 1 136 21 4 0.0 3 2 1 1 23 1 0.0 3 1 25 1 0.0 3 1 34 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 70 8 0.0 3 0 0 0 8 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 173 1 0.0 3 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.22 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 54,292 Reads with adapters: 2,105 (3.9%) Reads written (passing filters): 54,292 (100.0%) Total basepairs processed: 14,981,902 bp Total written (filtered): 14,975,274 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2105 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.6% C: 6.2% G: 24.4% T: 13.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1837 848.3 0 1837 4 224 212.1 0 224 5 43 53.0 0 43 6 1 13.3 0 1