This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 94.98 s (31 us/read; 1.95 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 131,867 (4.3%) Reads that were too short: 64,656 (2.1%) Reads written (passing filters): 67,211 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 10,663,542 bp (1.1%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 131867 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 22 755.5 0 22 7 48 755.5 0 48 8 1345 755.5 0 1345 9 2 755.5 0 2 10 6 755.5 0 6 11 3 755.5 0 3 12 4 755.5 0 4 13 1 755.5 0 1 14 7 755.5 0 7 15 3 755.5 0 3 16 19 755.5 0 19 17 5 755.5 0 5 18 9 755.5 0 9 19 3 755.5 0 3 20 2 755.5 0 2 21 4 755.5 0 4 23 6 755.5 0 6 24 5 755.5 0 5 25 8 755.5 0 8 26 99 755.5 0 99 27 78 755.5 0 78 28 79 755.5 0 79 29 5 755.5 0 5 30 3 755.5 0 3 31 4 755.5 0 4 32 3 755.5 0 3 33 4 755.5 0 4 35 4 755.5 0 4 36 6 755.5 0 6 37 3 755.5 0 3 38 4 755.5 0 4 39 5 755.5 0 5 40 8 755.5 0 8 41 5 755.5 0 5 42 10 755.5 0 10 43 6 755.5 0 6 44 3 755.5 0 3 45 4 755.5 0 4 46 4 755.5 0 4 47 8 755.5 0 8 48 5 755.5 0 5 49 3 755.5 0 3 50 6 755.5 0 6 51 1 755.5 0 1 52 2 755.5 0 2 53 3 755.5 0 3 54 3 755.5 0 3 55 3 755.5 0 3 56 1 755.5 0 1 58 2 755.5 0 2 59 3 755.5 0 3 60 6 755.5 0 6 61 10 755.5 0 10 62 3 755.5 0 3 63 46 755.5 0 46 64 5 755.5 0 5 65 4 755.5 0 4 66 11 755.5 0 11 67 3 755.5 0 3 68 1 755.5 0 1 69 8 755.5 0 8 70 4 755.5 0 4 71 27 755.5 0 27 72 9 755.5 0 9 73 5 755.5 0 5 74 8 755.5 0 8 75 10 755.5 0 10 76 5 755.5 0 5 77 3 755.5 0 3 78 10 755.5 0 10 79 43 755.5 0 43 80 69 755.5 0 69 81 152 755.5 0 152 82 160 755.5 0 160 83 160 755.5 0 160 84 164 755.5 0 164 85 342 755.5 0 342 86 145 755.5 0 145 87 42 755.5 0 42 88 110 755.5 0 110 89 273 755.5 0 273 90 36 755.5 0 36 91 177 755.5 0 177 92 127 755.5 0 127 93 98 755.5 0 98 94 101 755.5 0 101 95 758 755.5 0 758 96 983 755.5 0 983 97 483 755.5 0 483 98 285 755.5 0 285 99 319 755.5 0 319 100 165 755.5 0 165 101 291 755.5 0 291 102 539 755.5 0 539 103 284 755.5 0 284 104 147 755.5 0 147 105 736 755.5 0 736 106 504 755.5 0 504 107 431 755.5 0 431 108 2041 755.5 0 2041 109 1207 755.5 0 1207 110 704 755.5 0 704 111 462 755.5 0 462 112 1027 755.5 0 1027 113 656 755.5 0 656 114 1908 755.5 0 1908 115 1130 755.5 0 1130 116 373 755.5 0 373 117 330 755.5 0 330 118 174 755.5 0 174 119 109 755.5 0 109 120 149 755.5 0 149 121 317 755.5 0 317 122 674 755.5 0 674 123 531 755.5 0 531 124 713 755.5 0 713 125 238 755.5 0 238 126 433 755.5 0 433 127 404 755.5 0 404 128 329 755.5 0 329 129 725 755.5 0 725 130 485 755.5 0 485 131 545 755.5 0 545 132 502 755.5 0 502 133 383 755.5 0 383 134 386 755.5 0 386 135 422 755.5 0 422 136 778 755.5 0 778 137 969 755.5 0 969 138 638 755.5 0 638 139 473 755.5 0 473 140 448 755.5 0 448 141 996 755.5 0 996 142 687 755.5 0 687 143 458 755.5 0 458 144 270 755.5 0 270 145 292 755.5 0 292 146 466 755.5 0 466 147 547 755.5 0 547 148 1909 755.5 0 1909 149 692 755.5 0 692 150 875 755.5 0 875 151 315 755.5 0 315 152 340 755.5 0 340 153 655 755.5 0 655 154 362 755.5 0 362 155 659 755.5 0 659 156 624 755.5 0 624 157 722 755.5 0 722 158 472 755.5 0 472 159 384 755.5 0 384 160 437 755.5 0 437 161 555 755.5 0 555 162 358 755.5 0 358 163 1001 755.5 0 1001 164 570 755.5 0 570 165 855 755.5 0 855 166 563 755.5 0 563 167 615 755.5 0 615 168 524 755.5 0 524 169 624 755.5 0 624 170 494 755.5 0 494 171 231 755.5 0 231 172 430 755.5 0 430 173 251 755.5 0 251 174 499 755.5 0 499 175 691 755.5 0 691 176 995 755.5 0 995 177 375 755.5 0 375 178 268 755.5 0 268 179 210 755.5 0 210 180 201 755.5 0 201 181 501 755.5 0 501 182 508 755.5 0 508 183 544 755.5 0 544 184 433 755.5 0 433 185 588 755.5 0 588 186 957 755.5 0 957 187 880 755.5 0 880 188 525 755.5 0 525 189 258 755.5 0 258 190 476 755.5 0 476 191 667 755.5 0 667 192 855 755.5 0 855 193 935 755.5 0 935 194 1422 755.5 0 1422 195 367 755.5 0 367 196 535 755.5 0 535 197 244 755.5 0 244 198 371 755.5 0 371 199 844 755.5 0 844 200 1006 755.5 0 1006 201 979 755.5 0 979 202 952 755.5 0 952 203 307 755.5 0 307 204 358 755.5 0 358 205 655 755.5 0 655 206 733 755.5 0 733 207 418 755.5 0 418 208 1238 755.5 0 1238 209 991 755.5 0 991 210 711 755.5 0 711 211 603 755.5 0 603 212 351 755.5 0 351 213 458 755.5 0 458 214 399 755.5 0 399 215 350 755.5 0 350 216 445 755.5 0 445 217 438 755.5 0 438 218 555 755.5 0 555 219 865 755.5 0 865 220 523 755.5 0 523 221 440 755.5 0 440 222 713 755.5 0 713 223 338 755.5 0 338 224 295 755.5 0 295 225 348 755.5 0 348 226 469 755.5 0 469 227 557 755.5 0 557 228 680 755.5 0 680 229 776 755.5 0 776 230 1024 755.5 0 1024 231 658 755.5 0 658 232 398 755.5 0 398 233 338 755.5 0 338 234 285 755.5 0 285 235 361 755.5 0 361 236 682 755.5 0 682 237 1034 755.5 0 1034 238 914 755.5 0 914 239 707 755.5 0 707 240 610 755.5 0 610 241 331 755.5 0 331 242 387 755.5 0 387 243 399 755.5 0 399 244 696 755.5 0 696 245 826 755.5 0 826 246 1290 755.5 0 1290 247 1253 755.5 0 1253 248 1006 755.5 0 1006 249 621 755.5 0 621 250 230 755.5 0 230 251 166 755.5 0 166 252 141 755.5 0 141 253 386 755.5 0 386 254 429 755.5 0 429 255 515 755.5 0 515 256 701 755.5 0 701 257 1068 755.5 0 1068 258 2359 755.5 0 2359 259 1283 755.5 0 1283 260 682 755.5 0 682 261 303 755.5 0 303 262 261 755.5 0 261 263 435 755.5 0 435 264 621 755.5 0 621 265 584 755.5 0 584 266 599 755.5 0 599 267 1002 755.5 0 1002 268 987 755.5 0 987 269 2674 755.5 0 2674 270 912 755.5 0 912 271 701 755.5 0 701 272 362 755.5 0 362 273 484 755.5 0 484 274 577 755.5 0 577 275 642 755.5 0 642 276 629 755.5 0 629 277 372 755.5 0 372 278 292 755.5 0 292 279 416 755.5 0 416 280 785 755.5 0 785 281 720 755.5 0 720 282 887 755.5 0 887 283 400 755.5 0 400 284 376 755.5 0 376 285 534 755.5 0 534 286 596 755.5 0 596 287 939 755.5 0 939 288 925 755.5 0 925 289 808 755.5 0 808 290 454 755.5 0 454 291 297 755.5 0 297 292 402 755.5 0 402 293 663 755.5 0 663 294 695 755.5 0 695 295 1211 755.5 0 1211 296 885 755.5 0 885 297 617 755.5 0 617 298 443 755.5 0 443 299 390 755.5 0 390 300 307 755.5 0 307 301 723 755.5 0 723 Finished in 2.66 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 67,211 Reads with adapters: 1,411 (2.1%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 1,396 (2.1%) Total basepairs processed: 10,663,542 bp Total written (filtered): 378,105 bp (3.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1411 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 14 0.0 3 0 14 20 1347 0.0 4 1329 18 21 3 0.0 4 0 2 0 1 22 4 0.0 4 3 0 0 0 1 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 22 0.0 4 0 0 0 0 22 32 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 1 93 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,396 Reads with adapters: 28 (2.0%) Reads written (passing filters): 1,396 (100.0%) Total basepairs processed: 378,105 bp Total written (filtered): 377,287 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 3.6% G: 92.9% T: 0.0% none/other: 3.6% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 5 0.0 1 0 5 11 21 0.0 1 0 21 264 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.