This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.42 s (30 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 106,524 (3.4%) Reads that were too short: 3,398 (0.1%) Reads written (passing filters): 103,126 (3.3%) Total basepairs processed: 931,490,553 bp Total written (filtered): 18,349,333 bp (2.0%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 106524 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2427 755.5 0 2427 7 11803 755.5 0 11803 8 140 755.5 0 140 9 3054 755.5 0 3054 10 21 755.5 0 21 11 28 755.5 0 28 12 9 755.5 0 9 13 20 755.5 0 20 14 13 755.5 0 13 15 27 755.5 0 27 16 24 755.5 0 24 17 30 755.5 0 30 18 47 755.5 0 47 19 49 755.5 0 49 20 99 755.5 0 99 21 41 755.5 0 41 22 15 755.5 0 15 23 6 755.5 0 6 24 17 755.5 0 17 25 12 755.5 0 12 26 23 755.5 0 23 27 47 755.5 0 47 28 59 755.5 0 59 29 17 755.5 0 17 30 69 755.5 0 69 31 16 755.5 0 16 32 23 755.5 0 23 33 12 755.5 0 12 34 15 755.5 0 15 35 19 755.5 0 19 36 18 755.5 0 18 37 14 755.5 0 14 38 12 755.5 0 12 39 25 755.5 0 25 40 304 755.5 0 304 41 18 755.5 0 18 42 26 755.5 0 26 43 11 755.5 0 11 44 21 755.5 0 21 45 12 755.5 0 12 46 18 755.5 0 18 47 24 755.5 0 24 48 9 755.5 0 9 49 90 755.5 0 90 50 20 755.5 0 20 51 19 755.5 0 19 52 8 755.5 0 8 53 11 755.5 0 11 54 15 755.5 0 15 55 44 755.5 0 44 56 24 755.5 0 24 57 23 755.5 0 23 58 17 755.5 0 17 59 18 755.5 0 18 60 15 755.5 0 15 61 20 755.5 0 20 62 18 755.5 0 18 63 11 755.5 0 11 64 23 755.5 0 23 65 12 755.5 0 12 66 15 755.5 0 15 67 11 755.5 0 11 68 35 755.5 0 35 69 17 755.5 0 17 70 7 755.5 0 7 71 22 755.5 0 22 72 5 755.5 0 5 73 9 755.5 0 9 74 7 755.5 0 7 75 17 755.5 0 17 76 18 755.5 0 18 77 17 755.5 0 17 78 12 755.5 0 12 79 11 755.5 0 11 80 10 755.5 0 10 81 14 755.5 0 14 82 14 755.5 0 14 83 14 755.5 0 14 84 40 755.5 0 40 85 21 755.5 0 21 86 25 755.5 0 25 87 20 755.5 0 20 88 20 755.5 0 20 89 20 755.5 0 20 90 20 755.5 0 20 91 25 755.5 0 25 92 21 755.5 0 21 93 20 755.5 0 20 94 14 755.5 0 14 95 29 755.5 0 29 96 13 755.5 0 13 97 22 755.5 0 22 98 19 755.5 0 19 99 10 755.5 0 10 100 18 755.5 0 18 101 19 755.5 0 19 102 14 755.5 0 14 103 22 755.5 0 22 104 16 755.5 0 16 105 33 755.5 0 33 106 27 755.5 0 27 107 39 755.5 0 39 108 12 755.5 0 12 109 6 755.5 0 6 110 15 755.5 0 15 111 24 755.5 0 24 112 15 755.5 0 15 113 16 755.5 0 16 114 37 755.5 0 37 115 44 755.5 0 44 116 512 755.5 0 512 117 29 755.5 0 29 118 16 755.5 0 16 119 15 755.5 0 15 120 31 755.5 0 31 121 26 755.5 0 26 122 24 755.5 0 24 123 34 755.5 0 34 124 24 755.5 0 24 125 20 755.5 0 20 126 59 755.5 0 59 127 34 755.5 0 34 128 74 755.5 0 74 129 126 755.5 0 126 130 80 755.5 0 80 131 69 755.5 0 69 132 116 755.5 0 116 133 194 755.5 0 194 134 570 755.5 0 570 135 150 755.5 0 150 136 190 755.5 0 190 137 365 755.5 0 365 138 177 755.5 0 177 139 178 755.5 0 178 140 442 755.5 0 442 141 295 755.5 0 295 142 277 755.5 0 277 143 458 755.5 0 458 144 677 755.5 0 677 145 861 755.5 0 861 146 859 755.5 0 859 147 14469 755.5 0 14469 148 12329 755.5 0 12329 149 44860 755.5 0 44860 150 304 755.5 0 304 151 17 755.5 0 17 152 19 755.5 0 19 153 13 755.5 0 13 154 121 755.5 0 121 155 44 755.5 0 44 156 31 755.5 0 31 157 11 755.5 0 11 158 8 755.5 0 8 159 25 755.5 0 25 160 19 755.5 0 19 161 18 755.5 0 18 162 12 755.5 0 12 163 25 755.5 0 25 164 24 755.5 0 24 165 29 755.5 0 29 166 11 755.5 0 11 167 19 755.5 0 19 168 28 755.5 0 28 169 21 755.5 0 21 170 47 755.5 0 47 171 34 755.5 0 34 172 18 755.5 0 18 173 175 755.5 0 175 174 32 755.5 0 32 175 26 755.5 0 26 176 9 755.5 0 9 177 12 755.5 0 12 178 12 755.5 0 12 179 17 755.5 0 17 180 17 755.5 0 17 181 13 755.5 0 13 182 19 755.5 0 19 183 26 755.5 0 26 184 42 755.5 0 42 185 18 755.5 0 18 186 33 755.5 0 33 187 77 755.5 0 77 188 1567 755.5 0 1567 189 55 755.5 0 55 190 42 755.5 0 42 191 13 755.5 0 13 192 70 755.5 0 70 193 18 755.5 0 18 194 22 755.5 0 22 195 20 755.5 0 20 196 29 755.5 0 29 197 30 755.5 0 30 198 24 755.5 0 24 199 79 755.5 0 79 200 172 755.5 0 172 201 886 755.5 0 886 202 765 755.5 0 765 203 477 755.5 0 477 204 95 755.5 0 95 205 32 755.5 0 32 206 20 755.5 0 20 207 11 755.5 0 11 208 27 755.5 0 27 209 10 755.5 0 10 210 7 755.5 0 7 211 11 755.5 0 11 212 9 755.5 0 9 213 9 755.5 0 9 214 7 755.5 0 7 215 21 755.5 0 21 216 11 755.5 0 11 217 13 755.5 0 13 218 12 755.5 0 12 219 10 755.5 0 10 220 10 755.5 0 10 221 6 755.5 0 6 222 11 755.5 0 11 223 27 755.5 0 27 224 28 755.5 0 28 225 46 755.5 0 46 226 55 755.5 0 55 227 12 755.5 0 12 228 22 755.5 0 22 229 18 755.5 0 18 230 24 755.5 0 24 231 13 755.5 0 13 232 7 755.5 0 7 233 8 755.5 0 8 234 7 755.5 0 7 235 8 755.5 0 8 236 20 755.5 0 20 237 10 755.5 0 10 238 17 755.5 0 17 239 7 755.5 0 7 240 8 755.5 0 8 241 11 755.5 0 11 242 32 755.5 0 32 243 18 755.5 0 18 244 15 755.5 0 15 245 11 755.5 0 11 246 15 755.5 0 15 247 9 755.5 0 9 248 5 755.5 0 5 249 22 755.5 0 22 250 25 755.5 0 25 251 10 755.5 0 10 252 42 755.5 0 42 253 54 755.5 0 54 254 63 755.5 0 63 255 24 755.5 0 24 256 22 755.5 0 22 257 8 755.5 0 8 258 10 755.5 0 10 259 39 755.5 0 39 260 11 755.5 0 11 261 11 755.5 0 11 262 7 755.5 0 7 263 8 755.5 0 8 264 8 755.5 0 8 265 8 755.5 0 8 266 5 755.5 0 5 267 13 755.5 0 13 268 15 755.5 0 15 269 55 755.5 0 55 270 34 755.5 0 34 271 34 755.5 0 34 272 8 755.5 0 8 273 36 755.5 0 36 274 8 755.5 0 8 275 21 755.5 0 21 276 19 755.5 0 19 277 38 755.5 0 38 278 10 755.5 0 10 279 13 755.5 0 13 280 9 755.5 0 9 281 19 755.5 0 19 282 104 755.5 0 104 283 13 755.5 0 13 284 21 755.5 0 21 285 89 755.5 0 89 286 9 755.5 0 9 287 6 755.5 0 6 288 5 755.5 0 5 289 10 755.5 0 10 290 10 755.5 0 10 291 23 755.5 0 23 292 15 755.5 0 15 293 74 755.5 0 74 294 105 755.5 0 105 295 35 755.5 0 35 296 9 755.5 0 9 297 8 755.5 0 8 299 3 755.5 0 3 300 118 755.5 0 118 301 5 755.5 0 5 Finished in 3.85 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 103,126 Reads with adapters: 14,262 (13.8%) Reads that were too short: 71 (0.1%) Reads written (passing filters): 14,191 (13.8%) Total basepairs processed: 18,349,333 bp Total written (filtered): 3,910,404 bp (21.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14262 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 1 16 98 0.0 3 0 83 7 8 17 11693 0.0 3 11343 294 44 12 18 81 0.0 3 6 56 16 3 19 2197 0.0 3 2117 47 5 28 20 7 0.0 3 2 1 0 4 21 6 0.0 3 0 0 5 1 22 6 0.0 3 0 0 0 6 31 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 1 1 45 64 0.0 3 0 0 0 64 46 13 0.0 3 0 0 0 13 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 1 56 5 0.0 3 0 0 0 5 57 6 0.0 3 0 0 0 6 67 1 0.0 3 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 79 49 0.0 3 0 0 1 48 80 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 7 0.0 3 0 0 1 6 192 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 14,191 Reads with adapters: 1,449 (10.2%) Reads written (passing filters): 14,191 (100.0%) Total basepairs processed: 3,910,404 bp Total written (filtered): 3,905,908 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1449 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.6% C: 20.0% G: 36.4% T: 15.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1309 221.7 0 1309 4 135 55.4 0 135 5 2 13.9 0 2 6 2 3.5 0 2 7 1 0.9 0 1