This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.67 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 621,017 (20.1%) Reads that were too short: 257,562 (8.3%) Reads written (passing filters): 363,455 (11.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 61,377,826 bp (6.6%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 621017 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3295 755.5 0 3295 7 3018 755.5 0 3018 8 129 755.5 0 129 9 6116 755.5 0 6116 10 23 755.5 0 23 11 11 755.5 0 11 12 1 755.5 0 1 13 7 755.5 0 7 14 8 755.5 0 8 15 11 755.5 0 11 16 6 755.5 0 6 17 6 755.5 0 6 18 1 755.5 0 1 19 6 755.5 0 6 20 4 755.5 0 4 21 7 755.5 0 7 22 12 755.5 0 12 23 6 755.5 0 6 24 4 755.5 0 4 25 11 755.5 0 11 26 6 755.5 0 6 27 11 755.5 0 11 28 18 755.5 0 18 29 20 755.5 0 20 30 35 755.5 0 35 31 18 755.5 0 18 32 49 755.5 0 49 33 21 755.5 0 21 34 13 755.5 0 13 35 15 755.5 0 15 36 12 755.5 0 12 37 8 755.5 0 8 38 5 755.5 0 5 39 14 755.5 0 14 40 11 755.5 0 11 41 52 755.5 0 52 42 51 755.5 0 51 43 281 755.5 0 281 44 17 755.5 0 17 45 4 755.5 0 4 46 4 755.5 0 4 47 4 755.5 0 4 48 4 755.5 0 4 49 15 755.5 0 15 50 5 755.5 0 5 51 9 755.5 0 9 52 11 755.5 0 11 53 5 755.5 0 5 54 5 755.5 0 5 55 3 755.5 0 3 56 9 755.5 0 9 57 18 755.5 0 18 58 9 755.5 0 9 59 43 755.5 0 43 60 9 755.5 0 9 61 6 755.5 0 6 62 46 755.5 0 46 63 19 755.5 0 19 64 23 755.5 0 23 65 25 755.5 0 25 66 56 755.5 0 56 67 21 755.5 0 21 68 58 755.5 0 58 69 296 755.5 0 296 70 68 755.5 0 68 71 356 755.5 0 356 72 1539 755.5 0 1539 73 5988 755.5 0 5988 74 1831 755.5 0 1831 75 2696 755.5 0 2696 76 1420 755.5 0 1420 77 1587 755.5 0 1587 78 2628 755.5 0 2628 79 1914 755.5 0 1914 80 2635 755.5 0 2635 81 1601 755.5 0 1601 82 1543 755.5 0 1543 83 1051 755.5 0 1051 84 1065 755.5 0 1065 85 1618 755.5 0 1618 86 1927 755.5 0 1927 87 819 755.5 0 819 88 1117 755.5 0 1117 89 3394 755.5 0 3394 90 1757 755.5 0 1757 91 5528 755.5 0 5528 92 1635 755.5 0 1635 93 2173 755.5 0 2173 94 2090 755.5 0 2090 95 5182 755.5 0 5182 96 2274 755.5 0 2274 97 1422 755.5 0 1422 98 735 755.5 0 735 99 1235 755.5 0 1235 100 1210 755.5 0 1210 101 2804 755.5 0 2804 102 2828 755.5 0 2828 103 3454 755.5 0 3454 104 2298 755.5 0 2298 105 7859 755.5 0 7859 106 7105 755.5 0 7105 107 4947 755.5 0 4947 108 2388 755.5 0 2388 109 1957 755.5 0 1957 110 1612 755.5 0 1612 111 3518 755.5 0 3518 112 2932 755.5 0 2932 113 3628 755.5 0 3628 114 3455 755.5 0 3455 115 3405 755.5 0 3405 116 1414 755.5 0 1414 117 1943 755.5 0 1943 118 2090 755.5 0 2090 119 2910 755.5 0 2910 120 2754 755.5 0 2754 121 4272 755.5 0 4272 122 2193 755.5 0 2193 123 3647 755.5 0 3647 124 3191 755.5 0 3191 125 3318 755.5 0 3318 126 4118 755.5 0 4118 127 3588 755.5 0 3588 128 2716 755.5 0 2716 129 1829 755.5 0 1829 130 2483 755.5 0 2483 131 1661 755.5 0 1661 132 2989 755.5 0 2989 133 5232 755.5 0 5232 134 3861 755.5 0 3861 135 3226 755.5 0 3226 136 2451 755.5 0 2451 137 2078 755.5 0 2078 138 1828 755.5 0 1828 139 1905 755.5 0 1905 140 2488 755.5 0 2488 141 2891 755.5 0 2891 142 1939 755.5 0 1939 143 2355 755.5 0 2355 144 1988 755.5 0 1988 145 3167 755.5 0 3167 146 3261 755.5 0 3261 147 2553 755.5 0 2553 148 3765 755.5 0 3765 149 3015 755.5 0 3015 150 3687 755.5 0 3687 151 2020 755.5 0 2020 152 2872 755.5 0 2872 153 3678 755.5 0 3678 154 3321 755.5 0 3321 155 2794 755.5 0 2794 156 2259 755.5 0 2259 157 2045 755.5 0 2045 158 2459 755.5 0 2459 159 2313 755.5 0 2313 160 2415 755.5 0 2415 161 3066 755.5 0 3066 162 4069 755.5 0 4069 163 3561 755.5 0 3561 164 3583 755.5 0 3583 165 2845 755.5 0 2845 166 2426 755.5 0 2426 167 1957 755.5 0 1957 168 1922 755.5 0 1922 169 2516 755.5 0 2516 170 2558 755.5 0 2558 171 1940 755.5 0 1940 172 3125 755.5 0 3125 173 2701 755.5 0 2701 174 5327 755.5 0 5327 175 2717 755.5 0 2717 176 2286 755.5 0 2286 177 1356 755.5 0 1356 178 2367 755.5 0 2367 179 2324 755.5 0 2324 180 2873 755.5 0 2873 181 2511 755.5 0 2511 182 1893 755.5 0 1893 183 2120 755.5 0 2120 184 2292 755.5 0 2292 185 2777 755.5 0 2777 186 3425 755.5 0 3425 187 2783 755.5 0 2783 188 2602 755.5 0 2602 189 2065 755.5 0 2065 190 2681 755.5 0 2681 191 3154 755.5 0 3154 192 3230 755.5 0 3230 193 3071 755.5 0 3071 194 2667 755.5 0 2667 195 1258 755.5 0 1258 196 1503 755.5 0 1503 197 1524 755.5 0 1524 198 2866 755.5 0 2866 199 4407 755.5 0 4407 200 2326 755.5 0 2326 201 1525 755.5 0 1525 202 1659 755.5 0 1659 203 1683 755.5 0 1683 204 1808 755.5 0 1808 205 2045 755.5 0 2045 206 2404 755.5 0 2404 207 1845 755.5 0 1845 208 1926 755.5 0 1926 209 2348 755.5 0 2348 210 1742 755.5 0 1742 211 3012 755.5 0 3012 212 2246 755.5 0 2246 213 14943 755.5 0 14943 214 6281 755.5 0 6281 215 5530 755.5 0 5530 216 1973 755.5 0 1973 217 1986 755.5 0 1986 218 2624 755.5 0 2624 219 2907 755.5 0 2907 220 2493 755.5 0 2493 221 1688 755.5 0 1688 222 1813 755.5 0 1813 223 1854 755.5 0 1854 224 2568 755.5 0 2568 225 3145 755.5 0 3145 226 2412 755.5 0 2412 227 3411 755.5 0 3411 228 2352 755.5 0 2352 229 2670 755.5 0 2670 230 2881 755.5 0 2881 231 2419 755.5 0 2419 232 1525 755.5 0 1525 233 1540 755.5 0 1540 234 2398 755.5 0 2398 235 2772 755.5 0 2772 236 3114 755.5 0 3114 237 2771 755.5 0 2771 238 2511 755.5 0 2511 239 2777 755.5 0 2777 240 2126 755.5 0 2126 241 1512 755.5 0 1512 242 1604 755.5 0 1604 243 1534 755.5 0 1534 244 1735 755.5 0 1735 245 2000 755.5 0 2000 246 2487 755.5 0 2487 247 3475 755.5 0 3475 248 3389 755.5 0 3389 249 3161 755.5 0 3161 250 3551 755.5 0 3551 251 1823 755.5 0 1823 252 1546 755.5 0 1546 253 1952 755.5 0 1952 254 2117 755.5 0 2117 255 2869 755.5 0 2869 256 2985 755.5 0 2985 257 3441 755.5 0 3441 258 2438 755.5 0 2438 259 2602 755.5 0 2602 260 1983 755.5 0 1983 261 2106 755.5 0 2106 262 1799 755.5 0 1799 263 2734 755.5 0 2734 264 2492 755.5 0 2492 265 2575 755.5 0 2575 266 2322 755.5 0 2322 267 2375 755.5 0 2375 268 2161 755.5 0 2161 269 2167 755.5 0 2167 270 2012 755.5 0 2012 271 2188 755.5 0 2188 272 2766 755.5 0 2766 273 3431 755.5 0 3431 274 2732 755.5 0 2732 275 2363 755.5 0 2363 276 2266 755.5 0 2266 277 1736 755.5 0 1736 278 1815 755.5 0 1815 279 2558 755.5 0 2558 280 2896 755.5 0 2896 281 2154 755.5 0 2154 282 2841 755.5 0 2841 283 1906 755.5 0 1906 284 3975 755.5 0 3975 285 2897 755.5 0 2897 286 2785 755.5 0 2785 287 2289 755.5 0 2289 288 1606 755.5 0 1606 289 1744 755.5 0 1744 290 2067 755.5 0 2067 291 6264 755.5 0 6264 292 2509 755.5 0 2509 293 2292 755.5 0 2292 294 2255 755.5 0 2255 295 2189 755.5 0 2189 296 1782 755.5 0 1782 297 1322 755.5 0 1322 298 1118 755.5 0 1118 299 858 755.5 0 858 300 1797 755.5 0 1797 301 5012 755.5 0 5012 Finished in 12.48 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 363,455 Reads with adapters: 6,461 (1.8%) Reads that were too short: 82 (0.0%) Reads written (passing filters): 6,379 (1.8%) Total basepairs processed: 61,377,826 bp Total written (filtered): 1,743,446 bp (2.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6461 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 5 3 16 61 0.0 3 0 55 3 3 17 6181 0.0 3 6011 150 16 4 18 33 0.0 3 2 17 8 6 19 16 0.0 3 0 1 4 11 20 12 0.0 3 0 0 0 12 21 4 0.0 3 0 0 0 4 22 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 54 3 0.0 3 0 0 0 3 55 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 1 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 1 128 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 6,379 Reads with adapters: 498 (7.8%) Reads written (passing filters): 6,379 (100.0%) Total basepairs processed: 1,743,446 bp Total written (filtered): 1,741,896 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 498 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.0% C: 9.4% G: 19.5% T: 12.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 444 99.7 0 444 4 52 24.9 0 52 5 2 6.2 0 2