This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.38 s (29 us/read; 2.08 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 493,715 (16.0%) Reads that were too short: 478,779 (15.5%) Reads written (passing filters): 14,936 (0.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 3,532,994 bp (0.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 493715 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 118 755.5 0 118 7 153 755.5 0 153 8 7901 755.5 0 7901 9 42 755.5 0 42 10 19 755.5 0 19 11 13 755.5 0 13 12 12 755.5 0 12 13 33 755.5 0 33 14 18 755.5 0 18 15 24 755.5 0 24 16 28 755.5 0 28 17 19 755.5 0 19 18 50 755.5 0 50 19 27 755.5 0 27 20 50 755.5 0 50 21 29 755.5 0 29 22 45 755.5 0 45 23 23 755.5 0 23 24 20 755.5 0 20 25 14 755.5 0 14 26 25 755.5 0 25 27 19 755.5 0 19 28 24 755.5 0 24 29 14 755.5 0 14 30 13 755.5 0 13 31 28 755.5 0 28 32 19 755.5 0 19 33 81 755.5 0 81 34 17 755.5 0 17 35 21 755.5 0 21 36 20 755.5 0 20 37 21 755.5 0 21 38 29 755.5 0 29 39 26 755.5 0 26 40 20 755.5 0 20 41 13 755.5 0 13 42 22 755.5 0 22 43 24 755.5 0 24 44 22 755.5 0 22 45 27 755.5 0 27 46 21 755.5 0 21 47 9 755.5 0 9 48 20 755.5 0 20 49 13 755.5 0 13 50 24 755.5 0 24 51 25 755.5 0 25 52 38 755.5 0 38 53 41 755.5 0 41 54 13 755.5 0 13 55 14 755.5 0 14 56 6 755.5 0 6 57 15 755.5 0 15 58 14 755.5 0 14 59 12 755.5 0 12 60 22 755.5 0 22 61 9 755.5 0 9 62 11 755.5 0 11 63 39 755.5 0 39 64 26 755.5 0 26 65 26 755.5 0 26 66 28 755.5 0 28 67 34 755.5 0 34 68 33 755.5 0 33 69 26 755.5 0 26 70 17 755.5 0 17 71 21 755.5 0 21 72 28 755.5 0 28 73 25 755.5 0 25 74 26 755.5 0 26 75 25 755.5 0 25 76 21 755.5 0 21 77 36 755.5 0 36 78 19 755.5 0 19 79 20 755.5 0 20 80 15 755.5 0 15 81 22 755.5 0 22 82 26 755.5 0 26 83 19 755.5 0 19 84 34 755.5 0 34 85 20 755.5 0 20 86 12 755.5 0 12 87 22 755.5 0 22 88 20 755.5 0 20 89 53 755.5 0 53 90 30 755.5 0 30 91 33 755.5 0 33 92 32 755.5 0 32 93 54 755.5 0 54 94 22 755.5 0 22 95 27 755.5 0 27 96 24 755.5 0 24 97 19 755.5 0 19 98 16 755.5 0 16 99 17 755.5 0 17 100 16 755.5 0 16 101 19 755.5 0 19 102 14 755.5 0 14 103 14 755.5 0 14 104 15 755.5 0 15 105 15 755.5 0 15 106 13 755.5 0 13 107 13 755.5 0 13 108 15 755.5 0 15 109 14 755.5 0 14 110 14 755.5 0 14 111 13 755.5 0 13 112 10 755.5 0 10 113 14 755.5 0 14 114 16 755.5 0 16 115 11 755.5 0 11 116 18 755.5 0 18 117 14 755.5 0 14 118 9 755.5 0 9 119 11 755.5 0 11 120 15 755.5 0 15 121 18 755.5 0 18 122 23 755.5 0 23 123 18 755.5 0 18 124 21 755.5 0 21 125 48 755.5 0 48 126 61 755.5 0 61 127 60 755.5 0 60 128 23 755.5 0 23 129 18 755.5 0 18 130 19 755.5 0 19 131 39 755.5 0 39 132 194 755.5 0 194 133 53 755.5 0 53 134 24 755.5 0 24 135 17 755.5 0 17 136 21 755.5 0 21 137 12 755.5 0 12 138 15 755.5 0 15 139 13 755.5 0 13 140 7 755.5 0 7 141 21 755.5 0 21 142 12 755.5 0 12 143 12 755.5 0 12 144 21 755.5 0 21 145 20 755.5 0 20 146 24 755.5 0 24 147 23 755.5 0 23 148 23 755.5 0 23 149 15 755.5 0 15 150 12 755.5 0 12 151 13 755.5 0 13 152 10 755.5 0 10 153 11 755.5 0 11 154 14 755.5 0 14 155 27 755.5 0 27 156 24 755.5 0 24 157 21 755.5 0 21 158 11 755.5 0 11 159 12 755.5 0 12 160 12 755.5 0 12 161 18 755.5 0 18 162 14 755.5 0 14 163 14 755.5 0 14 164 19 755.5 0 19 165 18 755.5 0 18 166 19 755.5 0 19 167 16 755.5 0 16 168 34 755.5 0 34 169 14 755.5 0 14 170 23 755.5 0 23 171 28 755.5 0 28 172 27 755.5 0 27 173 64 755.5 0 64 174 66 755.5 0 66 175 53 755.5 0 53 176 34 755.5 0 34 177 17 755.5 0 17 178 11 755.5 0 11 179 29 755.5 0 29 180 57 755.5 0 57 181 150 755.5 0 150 182 76 755.5 0 76 183 24 755.5 0 24 184 22 755.5 0 22 185 210 755.5 0 210 186 48 755.5 0 48 187 47 755.5 0 47 188 34 755.5 0 34 189 37 755.5 0 37 190 28 755.5 0 28 191 53 755.5 0 53 192 165 755.5 0 165 193 729 755.5 0 729 194 222 755.5 0 222 195 425 755.5 0 425 196 50 755.5 0 50 197 33 755.5 0 33 198 17 755.5 0 17 199 21 755.5 0 21 200 66 755.5 0 66 201 172 755.5 0 172 202 44 755.5 0 44 203 22 755.5 0 22 204 12 755.5 0 12 205 28 755.5 0 28 206 51 755.5 0 51 207 43 755.5 0 43 208 41 755.5 0 41 209 26 755.5 0 26 210 25 755.5 0 25 211 38 755.5 0 38 212 56 755.5 0 56 213 96 755.5 0 96 214 58 755.5 0 58 215 116 755.5 0 116 216 85 755.5 0 85 217 117 755.5 0 117 218 184 755.5 0 184 219 434 755.5 0 434 220 165 755.5 0 165 221 238 755.5 0 238 222 476 755.5 0 476 223 360 755.5 0 360 224 719 755.5 0 719 225 563 755.5 0 563 226 455 755.5 0 455 227 664 755.5 0 664 228 588 755.5 0 588 229 794 755.5 0 794 230 1080 755.5 0 1080 231 1436 755.5 0 1436 232 10901 755.5 0 10901 233 11253 755.5 0 11253 234 34014 755.5 0 34014 235 1063 755.5 0 1063 236 888 755.5 0 888 237 5821 755.5 0 5821 238 9421 755.5 0 9421 239 79056 755.5 0 79056 240 890 755.5 0 890 241 474 755.5 0 474 242 433 755.5 0 433 243 1696 755.5 0 1696 244 1629 755.5 0 1629 245 498 755.5 0 498 246 482 755.5 0 482 247 1063 755.5 0 1063 248 582 755.5 0 582 249 657 755.5 0 657 250 1140 755.5 0 1140 251 2524 755.5 0 2524 252 1948 755.5 0 1948 253 2794 755.5 0 2794 254 3723 755.5 0 3723 255 2246 755.5 0 2246 256 2495 755.5 0 2495 257 16759 755.5 0 16759 258 31848 755.5 0 31848 259 231888 755.5 0 231888 260 7476 755.5 0 7476 261 53 755.5 0 53 262 22 755.5 0 22 263 10 755.5 0 10 264 12 755.5 0 12 265 12 755.5 0 12 266 9 755.5 0 9 267 12 755.5 0 12 268 23 755.5 0 23 269 13 755.5 0 13 270 15 755.5 0 15 271 4 755.5 0 4 272 9 755.5 0 9 273 5 755.5 0 5 274 4 755.5 0 4 275 5 755.5 0 5 276 11 755.5 0 11 277 6 755.5 0 6 278 7 755.5 0 7 279 11 755.5 0 11 280 8 755.5 0 8 281 14 755.5 0 14 282 12 755.5 0 12 283 16 755.5 0 16 284 27 755.5 0 27 285 82 755.5 0 82 286 342 755.5 0 342 287 2678 755.5 0 2678 288 28 755.5 0 28 289 9 755.5 0 9 290 93 755.5 0 93 291 245 755.5 0 245 292 94 755.5 0 94 293 61 755.5 0 61 294 11 755.5 0 11 295 6 755.5 0 6 296 3 755.5 0 3 297 38 755.5 0 38 298 24 755.5 0 24 299 16 755.5 0 16 300 15 755.5 0 15 301 38 755.5 0 38 Finished in 0.66 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 14,936 Reads with adapters: 8,079 (54.1%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 8,072 (54.0%) Total basepairs processed: 3,532,994 bp Total written (filtered): 2,227,365 bp (63.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8079 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 8 1 16 50 0.0 3 0 43 4 3 17 7911 0.0 3 7651 227 26 7 18 36 0.0 3 2 14 12 8 19 26 0.0 3 3 0 15 8 20 36 0.0 3 5 0 0 31 21 1 0.0 3 1 77 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 8,072 Reads with adapters: 413 (5.1%) Reads written (passing filters): 8,072 (100.0%) Total basepairs processed: 2,227,365 bp Total written (filtered): 2,226,085 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 413 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.1% C: 7.5% G: 25.4% T: 23.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 381 126.1 0 381 4 27 31.5 0 27 5 3 7.9 0 3 6 1 2.0 0 1 8 1 0.1 0 1