This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 93.95 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 102,952 (3.3%) Reads that were too short: 45,454 (1.5%) Reads written (passing filters): 57,498 (1.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 10,306,350 bp (1.1%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 102952 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 194 755.5 0 194 7 63 755.5 0 63 8 280 755.5 0 280 9 7226 755.5 0 7226 10 15 755.5 0 15 11 11 755.5 0 11 12 8 755.5 0 8 13 7 755.5 0 7 14 4 755.5 0 4 15 6 755.5 0 6 16 11 755.5 0 11 17 6 755.5 0 6 18 12 755.5 0 12 19 9 755.5 0 9 20 5 755.5 0 5 21 2 755.5 0 2 22 6 755.5 0 6 23 7 755.5 0 7 24 2 755.5 0 2 25 11 755.5 0 11 26 5 755.5 0 5 27 7 755.5 0 7 28 3 755.5 0 3 29 7 755.5 0 7 30 5 755.5 0 5 31 9 755.5 0 9 32 8 755.5 0 8 33 33 755.5 0 33 34 8 755.5 0 8 35 6 755.5 0 6 36 3 755.5 0 3 37 4 755.5 0 4 38 179 755.5 0 179 39 7 755.5 0 7 40 12 755.5 0 12 41 7 755.5 0 7 42 13 755.5 0 13 43 8 755.5 0 8 44 11 755.5 0 11 45 7 755.5 0 7 46 14 755.5 0 14 47 6 755.5 0 6 48 7 755.5 0 7 49 14 755.5 0 14 50 7 755.5 0 7 51 14 755.5 0 14 52 36 755.5 0 36 53 12 755.5 0 12 54 13 755.5 0 13 55 9 755.5 0 9 56 6 755.5 0 6 57 17 755.5 0 17 58 25 755.5 0 25 59 16 755.5 0 16 60 14 755.5 0 14 61 10 755.5 0 10 62 12 755.5 0 12 63 39 755.5 0 39 64 24 755.5 0 24 65 45 755.5 0 45 66 44 755.5 0 44 67 111 755.5 0 111 68 233 755.5 0 233 69 3681 755.5 0 3681 70 720 755.5 0 720 71 1094 755.5 0 1094 72 13 755.5 0 13 73 6 755.5 0 6 74 6 755.5 0 6 75 7 755.5 0 7 76 12 755.5 0 12 77 9 755.5 0 9 78 9 755.5 0 9 79 6 755.5 0 6 80 215 755.5 0 215 81 208 755.5 0 208 82 346 755.5 0 346 83 65 755.5 0 65 84 307 755.5 0 307 85 251 755.5 0 251 86 113 755.5 0 113 87 58 755.5 0 58 88 165 755.5 0 165 89 23 755.5 0 23 90 17 755.5 0 17 91 57 755.5 0 57 92 168 755.5 0 168 93 35 755.5 0 35 94 32 755.5 0 32 95 97 755.5 0 97 96 232 755.5 0 232 97 233 755.5 0 233 98 233 755.5 0 233 99 135 755.5 0 135 100 82 755.5 0 82 101 421 755.5 0 421 102 2314 755.5 0 2314 103 361 755.5 0 361 104 435 755.5 0 435 105 147 755.5 0 147 106 50 755.5 0 50 107 54 755.5 0 54 108 272 755.5 0 272 109 180 755.5 0 180 110 34 755.5 0 34 111 91 755.5 0 91 112 139 755.5 0 139 113 104 755.5 0 104 114 190 755.5 0 190 115 95 755.5 0 95 116 69 755.5 0 69 117 178 755.5 0 178 118 162 755.5 0 162 119 195 755.5 0 195 120 332 755.5 0 332 121 245 755.5 0 245 122 260 755.5 0 260 123 291 755.5 0 291 124 311 755.5 0 311 125 190 755.5 0 190 126 277 755.5 0 277 127 190 755.5 0 190 128 216 755.5 0 216 129 229 755.5 0 229 130 214 755.5 0 214 131 292 755.5 0 292 132 407 755.5 0 407 133 957 755.5 0 957 134 811 755.5 0 811 135 261 755.5 0 261 136 378 755.5 0 378 137 475 755.5 0 475 138 888 755.5 0 888 139 473 755.5 0 473 140 544 755.5 0 544 141 255 755.5 0 255 142 312 755.5 0 312 143 409 755.5 0 409 144 209 755.5 0 209 145 127 755.5 0 127 146 185 755.5 0 185 147 433 755.5 0 433 148 819 755.5 0 819 149 348 755.5 0 348 150 377 755.5 0 377 151 233 755.5 0 233 152 214 755.5 0 214 153 180 755.5 0 180 154 144 755.5 0 144 155 186 755.5 0 186 156 263 755.5 0 263 157 250 755.5 0 250 158 461 755.5 0 461 159 514 755.5 0 514 160 487 755.5 0 487 161 378 755.5 0 378 162 357 755.5 0 357 163 331 755.5 0 331 164 312 755.5 0 312 165 214 755.5 0 214 166 293 755.5 0 293 167 346 755.5 0 346 168 500 755.5 0 500 169 584 755.5 0 584 170 353 755.5 0 353 171 478 755.5 0 478 172 534 755.5 0 534 173 185 755.5 0 185 174 190 755.5 0 190 175 542 755.5 0 542 176 2018 755.5 0 2018 177 1260 755.5 0 1260 178 830 755.5 0 830 179 448 755.5 0 448 180 226 755.5 0 226 181 255 755.5 0 255 182 392 755.5 0 392 183 496 755.5 0 496 184 517 755.5 0 517 185 446 755.5 0 446 186 426 755.5 0 426 187 588 755.5 0 588 188 950 755.5 0 950 189 1425 755.5 0 1425 190 245 755.5 0 245 191 130 755.5 0 130 192 191 755.5 0 191 193 341 755.5 0 341 194 366 755.5 0 366 195 433 755.5 0 433 196 640 755.5 0 640 197 230 755.5 0 230 198 163 755.5 0 163 199 258 755.5 0 258 200 422 755.5 0 422 201 477 755.5 0 477 202 244 755.5 0 244 203 193 755.5 0 193 204 499 755.5 0 499 205 359 755.5 0 359 206 362 755.5 0 362 207 376 755.5 0 376 208 536 755.5 0 536 209 330 755.5 0 330 210 252 755.5 0 252 211 323 755.5 0 323 212 300 755.5 0 300 213 569 755.5 0 569 214 424 755.5 0 424 215 571 755.5 0 571 216 481 755.5 0 481 217 448 755.5 0 448 218 462 755.5 0 462 219 659 755.5 0 659 220 383 755.5 0 383 221 579 755.5 0 579 222 505 755.5 0 505 223 351 755.5 0 351 224 386 755.5 0 386 225 524 755.5 0 524 226 377 755.5 0 377 227 348 755.5 0 348 228 371 755.5 0 371 229 491 755.5 0 491 230 510 755.5 0 510 231 417 755.5 0 417 232 364 755.5 0 364 233 475 755.5 0 475 234 208 755.5 0 208 235 175 755.5 0 175 236 306 755.5 0 306 237 488 755.5 0 488 238 830 755.5 0 830 239 749 755.5 0 749 240 513 755.5 0 513 241 362 755.5 0 362 242 306 755.5 0 306 243 292 755.5 0 292 244 566 755.5 0 566 245 527 755.5 0 527 246 521 755.5 0 521 247 367 755.5 0 367 248 435 755.5 0 435 249 514 755.5 0 514 250 457 755.5 0 457 251 249 755.5 0 249 252 220 755.5 0 220 253 677 755.5 0 677 254 474 755.5 0 474 255 478 755.5 0 478 256 512 755.5 0 512 257 387 755.5 0 387 258 532 755.5 0 532 259 626 755.5 0 626 260 352 755.5 0 352 261 328 755.5 0 328 262 220 755.5 0 220 263 211 755.5 0 211 264 406 755.5 0 406 265 555 755.5 0 555 266 593 755.5 0 593 267 613 755.5 0 613 268 547 755.5 0 547 269 892 755.5 0 892 270 642 755.5 0 642 271 569 755.5 0 569 272 277 755.5 0 277 273 345 755.5 0 345 274 462 755.5 0 462 275 526 755.5 0 526 276 862 755.5 0 862 277 731 755.5 0 731 278 529 755.5 0 529 279 319 755.5 0 319 280 314 755.5 0 314 281 767 755.5 0 767 282 766 755.5 0 766 283 537 755.5 0 537 284 369 755.5 0 369 285 340 755.5 0 340 286 415 755.5 0 415 287 534 755.5 0 534 288 605 755.5 0 605 289 645 755.5 0 645 290 414 755.5 0 414 291 277 755.5 0 277 292 316 755.5 0 316 293 318 755.5 0 318 294 614 755.5 0 614 295 754 755.5 0 754 296 418 755.5 0 418 297 230 755.5 0 230 298 286 755.5 0 286 299 339 755.5 0 339 300 333 755.5 0 333 301 644 755.5 0 644 Finished in 2.44 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 57,498 Reads with adapters: 7,574 (13.2%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 7,562 (13.2%) Total basepairs processed: 10,306,350 bp Total written (filtered): 2,056,401 bp (20.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7574 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 6 0.0 3 0 0 5 1 19 141 0.0 3 0 139 1 0 1 20 7380 0.0 4 7273 101 6 21 16 0.0 4 4 10 0 0 2 22 4 0.0 4 1 0 2 0 1 23 5 0.0 4 1 0 0 2 2 24 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 49 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 1 132 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 7,562 Reads with adapters: 1,020 (13.5%) Reads written (passing filters): 7,562 (100.0%) Total basepairs processed: 2,056,401 bp Total written (filtered): 1,816,612 bp (88.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1020 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 90.0% C: 0.9% G: 8.7% T: 0.3% none/other: 0.1% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 10 0.0 0 0 10 10 56 0.0 1 4 52 11 26 0.0 1 0 26 72 27 0.0 1 21 6 73 2 0.0 1 1 1 207 1 0.0 1 1 208 1 0.0 1 1 223 3 0.0 1 3 228 8 0.0 1 6 2 231 1 0.0 1 1 237 1 0.0 1 1 255 1 0.0 1 1 257 304 0.0 1 289 15 258 14 0.0 1 12 2 259 3 0.0 1 3 260 4 0.0 1 4 265 1 0.0 1 1 267 2 0.0 1 2 268 529 0.0 1 517 12 269 19 0.0 1 19 270 5 0.0 1 5 271 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.