This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 93.16 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 645,959 (20.9%) Reads that were too short: 8,897 (0.3%) Reads written (passing filters): 637,062 (20.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 186,095,653 bp (20.0%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 645959 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 19874 755.5 0 19874 7 604645 755.5 0 604645 8 1118 755.5 0 1118 9 80 755.5 0 80 10 69 755.5 0 69 11 12 755.5 0 12 12 4 755.5 0 4 13 10 755.5 0 10 14 3 755.5 0 3 15 7 755.5 0 7 16 12 755.5 0 12 17 9 755.5 0 9 18 7 755.5 0 7 19 13 755.5 0 13 20 23 755.5 0 23 21 23 755.5 0 23 22 33 755.5 0 33 23 206 755.5 0 206 24 162 755.5 0 162 25 245 755.5 0 245 26 8 755.5 0 8 27 8 755.5 0 8 28 5 755.5 0 5 29 5 755.5 0 5 30 7 755.5 0 7 31 8 755.5 0 8 32 3 755.5 0 3 33 2 755.5 0 2 34 12 755.5 0 12 35 6 755.5 0 6 36 7 755.5 0 7 37 9 755.5 0 9 38 7 755.5 0 7 39 11 755.5 0 11 40 7 755.5 0 7 41 3 755.5 0 3 42 7 755.5 0 7 43 4 755.5 0 4 44 8 755.5 0 8 45 7 755.5 0 7 47 2 755.5 0 2 48 2 755.5 0 2 49 5 755.5 0 5 50 7 755.5 0 7 51 2 755.5 0 2 52 4 755.5 0 4 53 4 755.5 0 4 54 11 755.5 0 11 55 6 755.5 0 6 56 4 755.5 0 4 57 20 755.5 0 20 58 4 755.5 0 4 59 5 755.5 0 5 61 5 755.5 0 5 62 5 755.5 0 5 63 4 755.5 0 4 64 2 755.5 0 2 65 7 755.5 0 7 66 9 755.5 0 9 67 3 755.5 0 3 68 6 755.5 0 6 69 16 755.5 0 16 70 9 755.5 0 9 71 8 755.5 0 8 72 1 755.5 0 1 73 6 755.5 0 6 74 11 755.5 0 11 75 23 755.5 0 23 76 2 755.5 0 2 77 13 755.5 0 13 78 6 755.5 0 6 79 6 755.5 0 6 80 5 755.5 0 5 81 9 755.5 0 9 82 10 755.5 0 10 83 7 755.5 0 7 84 11 755.5 0 11 85 8 755.5 0 8 86 8 755.5 0 8 87 9 755.5 0 9 88 48 755.5 0 48 89 106 755.5 0 106 90 156 755.5 0 156 91 6 755.5 0 6 92 1 755.5 0 1 93 3 755.5 0 3 94 3 755.5 0 3 95 19 755.5 0 19 96 51 755.5 0 51 97 34 755.5 0 34 98 21 755.5 0 21 99 20 755.5 0 20 100 27 755.5 0 27 101 282 755.5 0 282 102 24 755.5 0 24 103 311 755.5 0 311 104 36 755.5 0 36 105 27 755.5 0 27 106 63 755.5 0 63 107 20 755.5 0 20 108 3 755.5 0 3 109 13 755.5 0 13 110 14 755.5 0 14 111 44 755.5 0 44 112 56 755.5 0 56 113 53 755.5 0 53 114 62 755.5 0 62 115 27 755.5 0 27 116 15 755.5 0 15 117 57 755.5 0 57 118 86 755.5 0 86 119 49 755.5 0 49 120 39 755.5 0 39 121 38 755.5 0 38 122 20 755.5 0 20 123 40 755.5 0 40 124 59 755.5 0 59 125 77 755.5 0 77 126 165 755.5 0 165 127 6013 755.5 0 6013 128 693 755.5 0 693 129 664 755.5 0 664 130 6 755.5 0 6 131 6 755.5 0 6 132 61 755.5 0 61 133 7 755.5 0 7 134 5 755.5 0 5 135 3 755.5 0 3 136 8 755.5 0 8 137 3 755.5 0 3 138 4 755.5 0 4 139 4 755.5 0 4 140 9 755.5 0 9 141 10 755.5 0 10 142 7 755.5 0 7 143 3 755.5 0 3 144 11 755.5 0 11 145 109 755.5 0 109 146 38 755.5 0 38 147 3 755.5 0 3 148 10 755.5 0 10 149 36 755.5 0 36 150 5 755.5 0 5 151 2 755.5 0 2 152 3 755.5 0 3 154 6 755.5 0 6 155 1 755.5 0 1 156 3 755.5 0 3 157 3 755.5 0 3 159 1 755.5 0 1 160 7 755.5 0 7 161 1 755.5 0 1 162 5 755.5 0 5 163 4 755.5 0 4 164 4 755.5 0 4 165 3 755.5 0 3 166 4 755.5 0 4 167 4 755.5 0 4 168 7 755.5 0 7 169 4 755.5 0 4 170 1 755.5 0 1 171 3 755.5 0 3 172 4 755.5 0 4 173 3 755.5 0 3 174 4 755.5 0 4 175 2 755.5 0 2 177 2 755.5 0 2 178 3 755.5 0 3 179 3 755.5 0 3 180 4 755.5 0 4 181 1 755.5 0 1 182 5 755.5 0 5 183 10 755.5 0 10 184 2 755.5 0 2 185 1 755.5 0 1 186 4 755.5 0 4 187 2 755.5 0 2 188 6 755.5 0 6 189 4 755.5 0 4 190 3 755.5 0 3 191 1 755.5 0 1 192 3 755.5 0 3 193 7 755.5 0 7 194 9 755.5 0 9 195 17 755.5 0 17 196 7 755.5 0 7 197 10 755.5 0 10 198 4 755.5 0 4 200 6 755.5 0 6 201 2 755.5 0 2 202 3 755.5 0 3 203 5 755.5 0 5 204 3 755.5 0 3 205 9 755.5 0 9 206 9 755.5 0 9 207 11 755.5 0 11 208 5 755.5 0 5 209 3 755.5 0 3 210 8 755.5 0 8 211 3 755.5 0 3 212 4 755.5 0 4 213 7 755.5 0 7 214 18 755.5 0 18 215 22 755.5 0 22 216 6 755.5 0 6 217 6 755.5 0 6 218 3 755.5 0 3 219 4 755.5 0 4 220 4 755.5 0 4 221 4 755.5 0 4 222 6 755.5 0 6 223 11 755.5 0 11 224 20 755.5 0 20 225 24 755.5 0 24 226 3 755.5 0 3 227 10 755.5 0 10 228 5 755.5 0 5 229 5 755.5 0 5 230 7 755.5 0 7 231 7 755.5 0 7 232 3 755.5 0 3 233 7 755.5 0 7 234 4 755.5 0 4 235 9 755.5 0 9 236 29 755.5 0 29 237 11 755.5 0 11 238 2 755.5 0 2 239 6 755.5 0 6 240 10 755.5 0 10 241 7 755.5 0 7 242 5 755.5 0 5 243 1 755.5 0 1 244 14 755.5 0 14 245 17 755.5 0 17 246 11 755.5 0 11 247 21 755.5 0 21 248 8 755.5 0 8 249 19 755.5 0 19 250 31 755.5 0 31 251 16 755.5 0 16 252 12 755.5 0 12 253 40 755.5 0 40 254 69 755.5 0 69 255 31 755.5 0 31 256 144 755.5 0 144 257 49 755.5 0 49 258 57 755.5 0 57 259 214 755.5 0 214 260 376 755.5 0 376 261 1154 755.5 0 1154 262 5392 755.5 0 5392 263 39 755.5 0 39 264 7 755.5 0 7 265 10 755.5 0 10 266 3 755.5 0 3 267 7 755.5 0 7 268 9 755.5 0 9 269 11 755.5 0 11 270 1 755.5 0 1 271 2 755.5 0 2 272 1 755.5 0 1 273 15 755.5 0 15 274 9 755.5 0 9 275 14 755.5 0 14 276 9 755.5 0 9 277 7 755.5 0 7 278 16 755.5 0 16 279 29 755.5 0 29 280 409 755.5 0 409 281 195 755.5 0 195 282 6 755.5 0 6 283 2 755.5 0 2 284 4 755.5 0 4 285 4 755.5 0 4 286 3 755.5 0 3 287 2 755.5 0 2 288 5 755.5 0 5 289 1 755.5 0 1 290 1 755.5 0 1 291 1 755.5 0 1 292 5 755.5 0 5 293 22 755.5 0 22 294 11 755.5 0 11 295 7 755.5 0 7 296 3 755.5 0 3 297 3 755.5 0 3 298 6 755.5 0 6 299 6 755.5 0 6 300 6 755.5 0 6 301 2 755.5 0 2 Finished in 28.08 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 637,062 Reads with adapters: 625,005 (98.1%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 625,005 (98.1%) Total basepairs processed: 186,095,653 bp Total written (filtered): 171,272,748 bp (92.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 625005 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 173 0.0 3 0 0 169 2 2 19 5825 0.0 3 0 5606 186 19 14 20 616949 0.0 4 606777 9737 332 41 62 21 1536 0.0 4 208 1312 15 0 1 22 122 0.0 4 9 5 97 2 9 23 373 0.0 4 4 4 3 346 16 24 13 0.0 4 4 2 0 2 5 25 3 0.0 4 0 0 2 1 26 4 0.0 4 1 1 2 27 1 0.0 4 1 28 1 0.0 4 1 30 1 0.0 4 0 1 34 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 24.08 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 625,005 Reads with adapters: 3,912 (0.6%) Reads written (passing filters): 625,005 (100.0%) Total basepairs processed: 171,272,748 bp Total written (filtered): 171,209,857 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3912 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 1.7% G: 93.1% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 57 9765.7 0 57 4 22 2441.4 0 22 5 16 610.4 0 16 6 3 152.6 0 3 9 1 2.4 0 1 10 8 0.6 1 0 8 11 3572 0.1 1 6 3566 12 62 0.0 1 2 60 13 3 0.0 1 1 2 15 6 0.0 1 0 6 18 1 0.0 1 1 35 2 0.0 1 1 1 58 47 0.0 1 43 4 59 4 0.0 1 4 61 6 0.0 1 4 2 62 1 0.0 1 1 64 1 0.0 1 1 103 1 0.0 1 1 149 1 0.0 1 1 151 1 0.0 1 1 152 30 0.0 1 4 26 153 2 0.0 1 0 2 159 1 0.0 1 1 162 2 0.0 1 2 163 1 0.0 1 1 164 1 0.0 1 1 191 4 0.0 1 0 4 192 1 0.0 1 1 193 1 0.0 1 0 1 209 1 0.0 1 0 1 210 46 0.0 1 36 10 211 2 0.0 1 1 1 225 1 0.0 1 1 230 1 0.0 1 1 234 1 0.0 1 1 265 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.