This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.93 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 89,679 (2.9%) Reads that were too short: 21,573 (0.7%) Reads written (passing filters): 68,106 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 17,574,031 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 89679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 470 755.5 0 470 7 647 755.5 0 647 8 14710 755.5 0 14710 9 34120 755.5 0 34120 10 93 755.5 0 93 11 3 755.5 0 3 12 4 755.5 0 4 13 4 755.5 0 4 15 2 755.5 0 2 16 2 755.5 0 2 17 3 755.5 0 3 18 4 755.5 0 4 19 3 755.5 0 3 20 5 755.5 0 5 21 3 755.5 0 3 22 2 755.5 0 2 23 2 755.5 0 2 24 3 755.5 0 3 25 1 755.5 0 1 26 2 755.5 0 2 27 9 755.5 0 9 28 21 755.5 0 21 29 5 755.5 0 5 30 1 755.5 0 1 31 1 755.5 0 1 32 6 755.5 0 6 33 5 755.5 0 5 34 13 755.5 0 13 35 4 755.5 0 4 36 4 755.5 0 4 37 3 755.5 0 3 38 6 755.5 0 6 39 12 755.5 0 12 40 6 755.5 0 6 41 6 755.5 0 6 42 10 755.5 0 10 43 8 755.5 0 8 44 5 755.5 0 5 45 8 755.5 0 8 46 1 755.5 0 1 47 2 755.5 0 2 48 10 755.5 0 10 49 15 755.5 0 15 50 9 755.5 0 9 51 6 755.5 0 6 52 11 755.5 0 11 53 4 755.5 0 4 54 14 755.5 0 14 55 16 755.5 0 16 56 4 755.5 0 4 57 13 755.5 0 13 58 34 755.5 0 34 59 91 755.5 0 91 60 1148 755.5 0 1148 61 113 755.5 0 113 62 298 755.5 0 298 63 15 755.5 0 15 64 126 755.5 0 126 65 42 755.5 0 42 66 718 755.5 0 718 67 309 755.5 0 309 68 49 755.5 0 49 69 2 755.5 0 2 70 4 755.5 0 4 71 9 755.5 0 9 72 8 755.5 0 8 73 8 755.5 0 8 74 7 755.5 0 7 75 7 755.5 0 7 76 15 755.5 0 15 77 71 755.5 0 71 78 15 755.5 0 15 79 99 755.5 0 99 80 51 755.5 0 51 81 109 755.5 0 109 82 196 755.5 0 196 83 69 755.5 0 69 84 64 755.5 0 64 85 50 755.5 0 50 86 64 755.5 0 64 87 23 755.5 0 23 88 19 755.5 0 19 89 52 755.5 0 52 90 23 755.5 0 23 91 82 755.5 0 82 92 127 755.5 0 127 93 39 755.5 0 39 94 65 755.5 0 65 95 59 755.5 0 59 96 28 755.5 0 28 97 184 755.5 0 184 98 35 755.5 0 35 99 41 755.5 0 41 100 8 755.5 0 8 101 16 755.5 0 16 102 20 755.5 0 20 103 39 755.5 0 39 104 18 755.5 0 18 105 14 755.5 0 14 106 14 755.5 0 14 107 12 755.5 0 12 108 48 755.5 0 48 109 43 755.5 0 43 110 13 755.5 0 13 111 37 755.5 0 37 112 83 755.5 0 83 113 127 755.5 0 127 114 125 755.5 0 125 115 754 755.5 0 754 116 606 755.5 0 606 117 160 755.5 0 160 118 36 755.5 0 36 119 12 755.5 0 12 120 47 755.5 0 47 121 60 755.5 0 60 122 59 755.5 0 59 123 87 755.5 0 87 124 66 755.5 0 66 125 65 755.5 0 65 126 45 755.5 0 45 127 78 755.5 0 78 128 57 755.5 0 57 129 55 755.5 0 55 130 68 755.5 0 68 131 41 755.5 0 41 132 47 755.5 0 47 133 60 755.5 0 60 134 62 755.5 0 62 135 52 755.5 0 52 136 75 755.5 0 75 137 56 755.5 0 56 138 87 755.5 0 87 139 89 755.5 0 89 140 53 755.5 0 53 141 54 755.5 0 54 142 66 755.5 0 66 143 106 755.5 0 106 144 83 755.5 0 83 145 64 755.5 0 64 146 101 755.5 0 101 147 42 755.5 0 42 148 72 755.5 0 72 149 50 755.5 0 50 150 79 755.5 0 79 151 93 755.5 0 93 152 62 755.5 0 62 153 40 755.5 0 40 154 64 755.5 0 64 155 68 755.5 0 68 156 98 755.5 0 98 157 64 755.5 0 64 158 86 755.5 0 86 159 80 755.5 0 80 160 95 755.5 0 95 161 102 755.5 0 102 162 79 755.5 0 79 163 94 755.5 0 94 164 136 755.5 0 136 165 134 755.5 0 134 166 391 755.5 0 391 167 435 755.5 0 435 168 106 755.5 0 106 169 76 755.5 0 76 170 114 755.5 0 114 171 76 755.5 0 76 172 121 755.5 0 121 173 65 755.5 0 65 174 59 755.5 0 59 175 62 755.5 0 62 176 140 755.5 0 140 177 99 755.5 0 99 178 85 755.5 0 85 179 115 755.5 0 115 180 86 755.5 0 86 181 174 755.5 0 174 182 105 755.5 0 105 183 107 755.5 0 107 184 102 755.5 0 102 185 127 755.5 0 127 186 236 755.5 0 236 187 158 755.5 0 158 188 163 755.5 0 163 189 2128 755.5 0 2128 190 551 755.5 0 551 191 769 755.5 0 769 192 113 755.5 0 113 193 95 755.5 0 95 194 156 755.5 0 156 195 99 755.5 0 99 196 117 755.5 0 117 197 122 755.5 0 122 198 70 755.5 0 70 199 63 755.5 0 63 200 112 755.5 0 112 201 114 755.5 0 114 202 120 755.5 0 120 203 113 755.5 0 113 204 69 755.5 0 69 205 82 755.5 0 82 206 107 755.5 0 107 207 103 755.5 0 103 208 117 755.5 0 117 209 121 755.5 0 121 210 67 755.5 0 67 211 144 755.5 0 144 212 143 755.5 0 143 213 119 755.5 0 119 214 105 755.5 0 105 215 88 755.5 0 88 216 137 755.5 0 137 217 161 755.5 0 161 218 97 755.5 0 97 219 136 755.5 0 136 220 216 755.5 0 216 221 126 755.5 0 126 222 83 755.5 0 83 223 36 755.5 0 36 224 55 755.5 0 55 225 133 755.5 0 133 226 145 755.5 0 145 227 115 755.5 0 115 228 130 755.5 0 130 229 110 755.5 0 110 230 143 755.5 0 143 231 143 755.5 0 143 232 157 755.5 0 157 233 302 755.5 0 302 234 184 755.5 0 184 235 97 755.5 0 97 236 687 755.5 0 687 237 391 755.5 0 391 238 217 755.5 0 217 239 137 755.5 0 137 240 116 755.5 0 116 241 110 755.5 0 110 242 83 755.5 0 83 243 85 755.5 0 85 244 106 755.5 0 106 245 992 755.5 0 992 246 2397 755.5 0 2397 247 2281 755.5 0 2281 248 161 755.5 0 161 249 147 755.5 0 147 250 108 755.5 0 108 251 40 755.5 0 40 252 32 755.5 0 32 253 37 755.5 0 37 254 77 755.5 0 77 255 166 755.5 0 166 256 365 755.5 0 365 257 193 755.5 0 193 258 134 755.5 0 134 259 259 755.5 0 259 260 612 755.5 0 612 261 248 755.5 0 248 262 221 755.5 0 221 263 72 755.5 0 72 264 79 755.5 0 79 265 108 755.5 0 108 266 146 755.5 0 146 267 483 755.5 0 483 268 387 755.5 0 387 269 249 755.5 0 249 270 256 755.5 0 256 271 194 755.5 0 194 272 400 755.5 0 400 273 88 755.5 0 88 274 102 755.5 0 102 275 143 755.5 0 143 276 143 755.5 0 143 277 124 755.5 0 124 278 172 755.5 0 172 279 239 755.5 0 239 280 157 755.5 0 157 281 161 755.5 0 161 282 146 755.5 0 146 283 150 755.5 0 150 284 371 755.5 0 371 285 125 755.5 0 125 286 117 755.5 0 117 287 149 755.5 0 149 288 173 755.5 0 173 289 101 755.5 0 101 290 145 755.5 0 145 291 99 755.5 0 99 292 89 755.5 0 89 293 124 755.5 0 124 294 226 755.5 0 226 295 645 755.5 0 645 296 270 755.5 0 270 297 87 755.5 0 87 298 60 755.5 0 60 299 78 755.5 0 78 300 93 755.5 0 93 301 16 755.5 0 16 Finished in 3.24 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 68,106 Reads with adapters: 14,238 (20.9%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 14,210 (20.9%) Total basepairs processed: 17,574,031 bp Total written (filtered): 3,916,780 bp (22.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14238 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 6 0.0 3 0 0 4 2 16 145 0.0 3 0 126 13 6 17 13858 0.0 3 13388 418 42 10 18 49 0.0 3 5 33 5 6 19 62 0.0 3 3 0 48 11 20 50 0.0 3 0 0 0 50 24 1 0.0 3 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 37 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 58 1 0.0 3 1 60 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 1 64 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 164 13 0.0 3 0 0 0 13 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 6 0.0 3 0 0 0 6 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 14,210 Reads with adapters: 315 (2.2%) Reads written (passing filters): 14,210 (100.0%) Total basepairs processed: 3,916,780 bp Total written (filtered): 3,915,728 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 315 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.6% C: 9.8% G: 32.7% T: 29.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 220 222.0 0 220 4 83 55.5 0 83 5 12 13.9 0 12