This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.88 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 376,928 (12.2%) Reads that were too short: 175,063 (5.7%) Reads written (passing filters): 201,865 (6.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 44,969,475 bp (4.8%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 376928 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 903 755.5 0 903 7 4616 755.5 0 4616 8 85058 755.5 0 85058 9 213 755.5 0 213 10 158 755.5 0 158 11 6114 755.5 0 6114 12 27 755.5 0 27 13 17 755.5 0 17 14 15 755.5 0 15 15 29 755.5 0 29 16 11 755.5 0 11 17 15 755.5 0 15 18 22 755.5 0 22 19 27 755.5 0 27 20 16 755.5 0 16 21 15 755.5 0 15 22 19 755.5 0 19 23 27 755.5 0 27 24 22 755.5 0 22 25 15 755.5 0 15 26 11 755.5 0 11 27 17 755.5 0 17 28 21 755.5 0 21 29 13 755.5 0 13 30 20 755.5 0 20 31 22 755.5 0 22 32 26 755.5 0 26 33 9 755.5 0 9 34 25 755.5 0 25 35 23 755.5 0 23 36 21 755.5 0 21 37 17 755.5 0 17 38 14 755.5 0 14 39 20 755.5 0 20 40 16 755.5 0 16 41 16 755.5 0 16 42 23 755.5 0 23 43 21 755.5 0 21 44 24 755.5 0 24 45 12 755.5 0 12 46 10 755.5 0 10 47 18 755.5 0 18 48 16 755.5 0 16 49 16 755.5 0 16 50 14 755.5 0 14 51 16 755.5 0 16 52 23 755.5 0 23 53 18 755.5 0 18 54 37 755.5 0 37 55 20 755.5 0 20 56 20 755.5 0 20 57 16 755.5 0 16 58 28 755.5 0 28 59 30 755.5 0 30 60 21 755.5 0 21 61 11 755.5 0 11 62 17 755.5 0 17 63 22 755.5 0 22 64 54 755.5 0 54 65 31 755.5 0 31 66 22 755.5 0 22 67 28 755.5 0 28 68 77 755.5 0 77 69 37 755.5 0 37 70 108 755.5 0 108 71 109 755.5 0 109 72 782 755.5 0 782 73 446 755.5 0 446 74 699 755.5 0 699 75 744 755.5 0 744 76 277 755.5 0 277 77 513 755.5 0 513 78 848 755.5 0 848 79 464 755.5 0 464 80 613 755.5 0 613 81 413 755.5 0 413 82 340 755.5 0 340 83 266 755.5 0 266 84 546 755.5 0 546 85 219 755.5 0 219 86 406 755.5 0 406 87 638 755.5 0 638 88 2399 755.5 0 2399 89 155 755.5 0 155 90 260 755.5 0 260 91 1681 755.5 0 1681 92 431 755.5 0 431 93 1035 755.5 0 1035 94 454 755.5 0 454 95 220 755.5 0 220 96 109 755.5 0 109 97 272 755.5 0 272 98 425 755.5 0 425 99 300 755.5 0 300 100 262 755.5 0 262 101 699 755.5 0 699 102 337 755.5 0 337 103 632 755.5 0 632 104 1773 755.5 0 1773 105 625 755.5 0 625 106 190 755.5 0 190 107 567 755.5 0 567 108 658 755.5 0 658 109 385 755.5 0 385 110 372 755.5 0 372 111 463 755.5 0 463 112 271 755.5 0 271 113 501 755.5 0 501 114 297 755.5 0 297 115 329 755.5 0 329 116 553 755.5 0 553 117 1303 755.5 0 1303 118 422 755.5 0 422 119 819 755.5 0 819 120 994 755.5 0 994 121 783 755.5 0 783 122 722 755.5 0 722 123 1387 755.5 0 1387 124 1101 755.5 0 1101 125 1028 755.5 0 1028 126 1402 755.5 0 1402 127 352 755.5 0 352 128 416 755.5 0 416 129 600 755.5 0 600 130 936 755.5 0 936 131 705 755.5 0 705 132 1325 755.5 0 1325 133 796 755.5 0 796 134 782 755.5 0 782 135 729 755.5 0 729 136 734 755.5 0 734 137 678 755.5 0 678 138 595 755.5 0 595 139 707 755.5 0 707 140 350 755.5 0 350 141 554 755.5 0 554 142 799 755.5 0 799 143 1302 755.5 0 1302 144 534 755.5 0 534 145 746 755.5 0 746 146 708 755.5 0 708 147 792 755.5 0 792 148 745 755.5 0 745 149 874 755.5 0 874 150 1121 755.5 0 1121 151 982 755.5 0 982 152 1163 755.5 0 1163 153 684 755.5 0 684 154 795 755.5 0 795 155 1128 755.5 0 1128 156 916 755.5 0 916 157 957 755.5 0 957 158 927 755.5 0 927 159 751 755.5 0 751 160 888 755.5 0 888 161 719 755.5 0 719 162 871 755.5 0 871 163 1030 755.5 0 1030 164 873 755.5 0 873 165 674 755.5 0 674 166 780 755.5 0 780 167 877 755.5 0 877 168 853 755.5 0 853 169 856 755.5 0 856 170 1464 755.5 0 1464 171 2692 755.5 0 2692 172 1486 755.5 0 1486 173 635 755.5 0 635 174 808 755.5 0 808 175 697 755.5 0 697 176 937 755.5 0 937 177 894 755.5 0 894 178 1623 755.5 0 1623 179 1345 755.5 0 1345 180 1184 755.5 0 1184 181 721 755.5 0 721 182 952 755.5 0 952 183 1044 755.5 0 1044 184 785 755.5 0 785 185 603 755.5 0 603 186 507 755.5 0 507 187 916 755.5 0 916 188 1734 755.5 0 1734 189 1567 755.5 0 1567 190 1218 755.5 0 1218 191 909 755.5 0 909 192 840 755.5 0 840 193 662 755.5 0 662 194 699 755.5 0 699 195 2089 755.5 0 2089 196 1261 755.5 0 1261 197 445 755.5 0 445 198 539 755.5 0 539 199 567 755.5 0 567 200 919 755.5 0 919 201 1160 755.5 0 1160 202 875 755.5 0 875 203 681 755.5 0 681 204 732 755.5 0 732 205 587 755.5 0 587 206 340 755.5 0 340 207 765 755.5 0 765 208 1044 755.5 0 1044 209 587 755.5 0 587 210 459 755.5 0 459 211 763 755.5 0 763 212 552 755.5 0 552 213 671 755.5 0 671 214 1550 755.5 0 1550 215 2185 755.5 0 2185 216 1439 755.5 0 1439 217 1267 755.5 0 1267 218 847 755.5 0 847 219 458 755.5 0 458 220 542 755.5 0 542 221 1666 755.5 0 1666 222 1497 755.5 0 1497 223 974 755.5 0 974 224 1132 755.5 0 1132 225 1813 755.5 0 1813 226 4771 755.5 0 4771 227 9542 755.5 0 9542 228 1814 755.5 0 1814 229 3071 755.5 0 3071 230 1675 755.5 0 1675 231 3264 755.5 0 3264 232 2211 755.5 0 2211 233 2601 755.5 0 2601 234 637 755.5 0 637 235 465 755.5 0 465 236 798 755.5 0 798 237 899 755.5 0 899 238 936 755.5 0 936 239 945 755.5 0 945 240 888 755.5 0 888 241 958 755.5 0 958 242 1334 755.5 0 1334 243 1004 755.5 0 1004 244 822 755.5 0 822 245 612 755.5 0 612 246 480 755.5 0 480 247 588 755.5 0 588 248 1282 755.5 0 1282 249 1985 755.5 0 1985 250 2029 755.5 0 2029 251 1775 755.5 0 1775 252 2274 755.5 0 2274 253 2142 755.5 0 2142 254 1076 755.5 0 1076 255 889 755.5 0 889 256 620 755.5 0 620 257 948 755.5 0 948 258 725 755.5 0 725 259 901 755.5 0 901 260 855 755.5 0 855 261 1331 755.5 0 1331 262 1669 755.5 0 1669 263 2552 755.5 0 2552 264 8355 755.5 0 8355 265 3197 755.5 0 3197 266 4212 755.5 0 4212 267 882 755.5 0 882 268 1560 755.5 0 1560 269 1070 755.5 0 1070 270 898 755.5 0 898 271 1287 755.5 0 1287 272 1296 755.5 0 1296 273 1887 755.5 0 1887 274 3705 755.5 0 3705 275 2630 755.5 0 2630 276 2263 755.5 0 2263 277 1811 755.5 0 1811 278 1268 755.5 0 1268 279 797 755.5 0 797 280 1236 755.5 0 1236 281 2704 755.5 0 2704 282 1824 755.5 0 1824 283 1288 755.5 0 1288 284 1737 755.5 0 1737 285 1185 755.5 0 1185 286 909 755.5 0 909 287 980 755.5 0 980 288 777 755.5 0 777 289 1215 755.5 0 1215 290 1066 755.5 0 1066 291 3397 755.5 0 3397 292 5305 755.5 0 5305 293 23122 755.5 0 23122 294 651 755.5 0 651 295 524 755.5 0 524 296 595 755.5 0 595 297 653 755.5 0 653 298 415 755.5 0 415 299 467 755.5 0 467 300 577 755.5 0 577 301 524 755.5 0 524 Finished in 8.40 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 201,865 Reads with adapters: 2,756 (1.4%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 2,690 (1.3%) Total basepairs processed: 44,969,475 bp Total written (filtered): 723,301 bp (1.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2756 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 33 0.0 3 0 30 2 1 17 2504 0.0 3 2424 64 9 7 18 3 0.0 3 0 2 1 19 5 0.0 3 0 0 2 3 20 1 0.0 3 0 0 0 1 22 3 0.0 3 0 0 0 3 23 5 0.0 3 0 0 0 5 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 15 0.0 3 0 0 0 15 68 1 0.0 3 0 0 0 1 70 5 0.0 3 0 0 0 5 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 1 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 1 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 1 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 3 0.0 3 0 0 0 3 164 46 0.0 3 0 0 0 46 182 15 0.0 3 0 0 0 15 183 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 192 11 0.0 3 0 0 0 11 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 274 3 0.0 3 0 0 0 3 277 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 7 0.0 3 0 0 0 7 282 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,690 Reads with adapters: 288 (10.7%) Reads written (passing filters): 2,690 (100.0%) Total basepairs processed: 723,301 bp Total written (filtered): 722,428 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 288 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 60.4% G: 11.5% T: 11.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 279 42.0 0 279 4 9 10.5 0 9