This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.93 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 220,218 (7.1%) Reads that were too short: 23,464 (0.8%) Reads written (passing filters): 196,754 (6.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 51,588,406 bp (5.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 220218 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4446 755.5 0 4446 7 129216 755.5 0 129216 8 180 755.5 0 180 9 7083 755.5 0 7083 10 15 755.5 0 15 11 4 755.5 0 4 12 5 755.5 0 5 13 1 755.5 0 1 14 5 755.5 0 5 15 2 755.5 0 2 16 2 755.5 0 2 17 1 755.5 0 1 18 4 755.5 0 4 19 7 755.5 0 7 20 9 755.5 0 9 21 89 755.5 0 89 22 8 755.5 0 8 23 42 755.5 0 42 24 5 755.5 0 5 25 39 755.5 0 39 26 8 755.5 0 8 27 6 755.5 0 6 28 1 755.5 0 1 29 4 755.5 0 4 30 6 755.5 0 6 31 5 755.5 0 5 32 29 755.5 0 29 33 22 755.5 0 22 34 1445 755.5 0 1445 35 272 755.5 0 272 36 10 755.5 0 10 37 4 755.5 0 4 38 4 755.5 0 4 39 2 755.5 0 2 40 7 755.5 0 7 41 2 755.5 0 2 42 4 755.5 0 4 43 2 755.5 0 2 44 3 755.5 0 3 45 4 755.5 0 4 46 6 755.5 0 6 47 3 755.5 0 3 48 2 755.5 0 2 49 3 755.5 0 3 50 4 755.5 0 4 51 2 755.5 0 2 52 1 755.5 0 1 53 28 755.5 0 28 54 9 755.5 0 9 55 4 755.5 0 4 56 4 755.5 0 4 57 6 755.5 0 6 58 6 755.5 0 6 59 4 755.5 0 4 60 8 755.5 0 8 61 8 755.5 0 8 62 46 755.5 0 46 63 6 755.5 0 6 64 2 755.5 0 2 65 2 755.5 0 2 66 1 755.5 0 1 68 13 755.5 0 13 69 20 755.5 0 20 70 4 755.5 0 4 71 4 755.5 0 4 72 8 755.5 0 8 73 3 755.5 0 3 74 20 755.5 0 20 75 13 755.5 0 13 76 6 755.5 0 6 77 12 755.5 0 12 78 56 755.5 0 56 79 74 755.5 0 74 80 66 755.5 0 66 81 59 755.5 0 59 82 117 755.5 0 117 83 102 755.5 0 102 84 65 755.5 0 65 85 115 755.5 0 115 86 78 755.5 0 78 87 143 755.5 0 143 88 96 755.5 0 96 89 27 755.5 0 27 90 69 755.5 0 69 91 36 755.5 0 36 92 21 755.5 0 21 93 53 755.5 0 53 94 117 755.5 0 117 95 199 755.5 0 199 96 317 755.5 0 317 97 339 755.5 0 339 98 525 755.5 0 525 99 333 755.5 0 333 100 275 755.5 0 275 101 541 755.5 0 541 102 167 755.5 0 167 103 93 755.5 0 93 104 187 755.5 0 187 105 157 755.5 0 157 106 138 755.5 0 138 107 203 755.5 0 203 108 381 755.5 0 381 109 401 755.5 0 401 110 398 755.5 0 398 111 8497 755.5 0 8497 112 4625 755.5 0 4625 113 19500 755.5 0 19500 114 1062 755.5 0 1062 115 369 755.5 0 369 116 95 755.5 0 95 117 52 755.5 0 52 118 53 755.5 0 53 119 66 755.5 0 66 120 99 755.5 0 99 121 82 755.5 0 82 122 56 755.5 0 56 123 57 755.5 0 57 124 59 755.5 0 59 125 67 755.5 0 67 126 76 755.5 0 76 127 100 755.5 0 100 128 108 755.5 0 108 129 291 755.5 0 291 130 199 755.5 0 199 131 472 755.5 0 472 132 176 755.5 0 176 133 245 755.5 0 245 134 167 755.5 0 167 135 127 755.5 0 127 136 108 755.5 0 108 137 86 755.5 0 86 138 116 755.5 0 116 139 133 755.5 0 133 140 147 755.5 0 147 141 114 755.5 0 114 142 162 755.5 0 162 143 194 755.5 0 194 144 1342 755.5 0 1342 145 722 755.5 0 722 146 142 755.5 0 142 147 309 755.5 0 309 148 151 755.5 0 151 149 165 755.5 0 165 150 109 755.5 0 109 151 80 755.5 0 80 152 58 755.5 0 58 153 60 755.5 0 60 154 69 755.5 0 69 155 102 755.5 0 102 156 106 755.5 0 106 157 100 755.5 0 100 158 176 755.5 0 176 159 130 755.5 0 130 160 149 755.5 0 149 161 111 755.5 0 111 162 128 755.5 0 128 163 111 755.5 0 111 164 106 755.5 0 106 165 119 755.5 0 119 166 128 755.5 0 128 167 155 755.5 0 155 168 151 755.5 0 151 169 179 755.5 0 179 170 101 755.5 0 101 171 134 755.5 0 134 172 63 755.5 0 63 173 64 755.5 0 64 174 53 755.5 0 53 175 181 755.5 0 181 176 111 755.5 0 111 177 115 755.5 0 115 178 62 755.5 0 62 179 59 755.5 0 59 180 65 755.5 0 65 181 156 755.5 0 156 182 187 755.5 0 187 183 176 755.5 0 176 184 148 755.5 0 148 185 216 755.5 0 216 186 279 755.5 0 279 187 221 755.5 0 221 188 166 755.5 0 166 189 146 755.5 0 146 190 62 755.5 0 62 191 66 755.5 0 66 192 104 755.5 0 104 193 324 755.5 0 324 194 244 755.5 0 244 195 309 755.5 0 309 196 151 755.5 0 151 197 79 755.5 0 79 198 88 755.5 0 88 199 163 755.5 0 163 200 188 755.5 0 188 201 146 755.5 0 146 202 64 755.5 0 64 203 59 755.5 0 59 204 116 755.5 0 116 205 268 755.5 0 268 206 282 755.5 0 282 207 290 755.5 0 290 208 186 755.5 0 186 209 114 755.5 0 114 210 103 755.5 0 103 211 110 755.5 0 110 212 191 755.5 0 191 213 238 755.5 0 238 214 226 755.5 0 226 215 228 755.5 0 228 216 186 755.5 0 186 217 182 755.5 0 182 218 180 755.5 0 180 219 288 755.5 0 288 220 252 755.5 0 252 221 230 755.5 0 230 222 111 755.5 0 111 223 80 755.5 0 80 224 145 755.5 0 145 225 185 755.5 0 185 226 235 755.5 0 235 227 225 755.5 0 225 228 258 755.5 0 258 229 349 755.5 0 349 230 277 755.5 0 277 231 208 755.5 0 208 232 162 755.5 0 162 233 104 755.5 0 104 234 104 755.5 0 104 235 187 755.5 0 187 236 329 755.5 0 329 237 296 755.5 0 296 238 225 755.5 0 225 239 208 755.5 0 208 240 187 755.5 0 187 241 159 755.5 0 159 242 88 755.5 0 88 243 115 755.5 0 115 244 142 755.5 0 142 245 227 755.5 0 227 246 299 755.5 0 299 247 284 755.5 0 284 248 368 755.5 0 368 249 183 755.5 0 183 250 140 755.5 0 140 251 58 755.5 0 58 252 56 755.5 0 56 253 76 755.5 0 76 254 134 755.5 0 134 255 162 755.5 0 162 256 209 755.5 0 209 257 286 755.5 0 286 258 690 755.5 0 690 259 325 755.5 0 325 260 204 755.5 0 204 261 118 755.5 0 118 262 99 755.5 0 99 263 143 755.5 0 143 264 103 755.5 0 103 265 114 755.5 0 114 266 177 755.5 0 177 267 344 755.5 0 344 268 456 755.5 0 456 269 509 755.5 0 509 270 341 755.5 0 341 271 133 755.5 0 133 272 134 755.5 0 134 273 183 755.5 0 183 274 254 755.5 0 254 275 284 755.5 0 284 276 338 755.5 0 338 277 205 755.5 0 205 278 105 755.5 0 105 279 185 755.5 0 185 280 324 755.5 0 324 281 419 755.5 0 419 282 711 755.5 0 711 283 263 755.5 0 263 284 390 755.5 0 390 285 107 755.5 0 107 286 172 755.5 0 172 287 202 755.5 0 202 288 349 755.5 0 349 289 190 755.5 0 190 290 315 755.5 0 315 291 269 755.5 0 269 292 689 755.5 0 689 293 330 755.5 0 330 294 424 755.5 0 424 295 367 755.5 0 367 296 280 755.5 0 280 297 146 755.5 0 146 298 232 755.5 0 232 299 243 755.5 0 243 300 591 755.5 0 591 301 353 755.5 0 353 Finished in 9.39 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 196,754 Reads with adapters: 7,448 (3.8%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 7,368 (3.7%) Total basepairs processed: 51,588,406 bp Total written (filtered): 2,013,399 bp (3.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7448 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 95 0.0 3 0 91 4 17 7097 0.0 3 6813 240 34 10 18 26 0.0 3 7 13 4 2 19 41 0.0 3 32 1 3 5 20 17 0.0 3 7 2 0 8 22 1 0.0 3 0 0 0 1 23 1 0.0 3 1 29 7 0.0 3 0 0 0 7 32 1 0.0 3 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 1 82 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 1 115 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 11 0.0 3 0 0 0 11 142 2 0.0 3 0 0 0 2 144 17 0.0 3 0 0 0 17 145 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 60 0.0 3 0 0 0 60 185 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 7 0.0 3 0 0 0 7 282 9 0.0 3 0 0 0 9 283 10 0.0 3 0 0 0 10 289 2 0.0 3 0 0 0 2 291 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 7,368 Reads with adapters: 1,349 (18.3%) Reads written (passing filters): 7,368 (100.0%) Total basepairs processed: 2,013,399 bp Total written (filtered): 2,009,268 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1349 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.3% C: 8.7% G: 9.0% T: 8.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1274 115.1 0 1274 4 74 28.8 0 74 13 1 0.0 1 0 1