This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.01 s (29 us/read; 2.06 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 27,514 (0.9%) Reads that were too short: 3,160 (0.1%) Reads written (passing filters): 24,354 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 5,051,878 bp (0.5%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 27514 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 619 755.5 0 619 7 8296 755.5 0 8296 8 29 755.5 0 29 9 16 755.5 0 16 10 8 755.5 0 8 11 5 755.5 0 5 12 9 755.5 0 9 13 7 755.5 0 7 14 6 755.5 0 6 15 7 755.5 0 7 16 6 755.5 0 6 17 9 755.5 0 9 18 9 755.5 0 9 19 9 755.5 0 9 20 14 755.5 0 14 21 6 755.5 0 6 22 12 755.5 0 12 23 7 755.5 0 7 24 8 755.5 0 8 25 6 755.5 0 6 26 7 755.5 0 7 27 19 755.5 0 19 28 11 755.5 0 11 29 10 755.5 0 10 30 546 755.5 0 546 31 60 755.5 0 60 32 40 755.5 0 40 33 496 755.5 0 496 34 114 755.5 0 114 35 104 755.5 0 104 36 12 755.5 0 12 37 8 755.5 0 8 38 14 755.5 0 14 39 9 755.5 0 9 40 3 755.5 0 3 41 9 755.5 0 9 42 4 755.5 0 4 43 6 755.5 0 6 44 7 755.5 0 7 45 5 755.5 0 5 46 9 755.5 0 9 47 12 755.5 0 12 48 10 755.5 0 10 49 9 755.5 0 9 50 11 755.5 0 11 51 6 755.5 0 6 52 5 755.5 0 5 53 23 755.5 0 23 54 14 755.5 0 14 55 54 755.5 0 54 56 16 755.5 0 16 57 27 755.5 0 27 58 107 755.5 0 107 59 68 755.5 0 68 60 12 755.5 0 12 61 8 755.5 0 8 62 3 755.5 0 3 63 7 755.5 0 7 64 11 755.5 0 11 65 10 755.5 0 10 66 8 755.5 0 8 67 6 755.5 0 6 68 10 755.5 0 10 69 5 755.5 0 5 70 6 755.5 0 6 71 11 755.5 0 11 72 110 755.5 0 110 73 53 755.5 0 53 74 118 755.5 0 118 75 7 755.5 0 7 76 10 755.5 0 10 77 14 755.5 0 14 78 8 755.5 0 8 79 11 755.5 0 11 80 10 755.5 0 10 81 13 755.5 0 13 82 6 755.5 0 6 83 14 755.5 0 14 84 75 755.5 0 75 85 11 755.5 0 11 86 21 755.5 0 21 87 5 755.5 0 5 88 22 755.5 0 22 89 8 755.5 0 8 90 22 755.5 0 22 91 8 755.5 0 8 92 13 755.5 0 13 93 7 755.5 0 7 94 23 755.5 0 23 95 9 755.5 0 9 96 20 755.5 0 20 97 17 755.5 0 17 98 19 755.5 0 19 99 21 755.5 0 21 100 9 755.5 0 9 101 15 755.5 0 15 102 16 755.5 0 16 103 14 755.5 0 14 104 16 755.5 0 16 105 13 755.5 0 13 106 34 755.5 0 34 107 374 755.5 0 374 108 461 755.5 0 461 109 847 755.5 0 847 110 98 755.5 0 98 111 7 755.5 0 7 112 5 755.5 0 5 113 24 755.5 0 24 114 12 755.5 0 12 115 5 755.5 0 5 116 16 755.5 0 16 117 14 755.5 0 14 118 5 755.5 0 5 119 4 755.5 0 4 120 13 755.5 0 13 121 37 755.5 0 37 122 22 755.5 0 22 123 21 755.5 0 21 124 11 755.5 0 11 125 8 755.5 0 8 126 6 755.5 0 6 127 19 755.5 0 19 128 61 755.5 0 61 129 242 755.5 0 242 130 49 755.5 0 49 131 17 755.5 0 17 132 13 755.5 0 13 133 20 755.5 0 20 134 13 755.5 0 13 135 28 755.5 0 28 136 150 755.5 0 150 137 72 755.5 0 72 138 54 755.5 0 54 139 21 755.5 0 21 140 11 755.5 0 11 141 30 755.5 0 30 142 64 755.5 0 64 143 44 755.5 0 44 144 619 755.5 0 619 145 84 755.5 0 84 146 17 755.5 0 17 147 48 755.5 0 48 148 8 755.5 0 8 149 16 755.5 0 16 150 11 755.5 0 11 151 8 755.5 0 8 152 21 755.5 0 21 153 33 755.5 0 33 154 29 755.5 0 29 155 31 755.5 0 31 156 46 755.5 0 46 157 40 755.5 0 40 158 33 755.5 0 33 159 392 755.5 0 392 160 15 755.5 0 15 161 11 755.5 0 11 162 20 755.5 0 20 163 13 755.5 0 13 164 24 755.5 0 24 165 14 755.5 0 14 166 16 755.5 0 16 167 33 755.5 0 33 168 32 755.5 0 32 169 149 755.5 0 149 170 48 755.5 0 48 171 31 755.5 0 31 172 8 755.5 0 8 173 12 755.5 0 12 174 28 755.5 0 28 175 30 755.5 0 30 176 18 755.5 0 18 177 6 755.5 0 6 178 9 755.5 0 9 179 18 755.5 0 18 180 29 755.5 0 29 181 36 755.5 0 36 182 59 755.5 0 59 183 79 755.5 0 79 184 124 755.5 0 124 185 302 755.5 0 302 186 4895 755.5 0 4895 187 796 755.5 0 796 188 1211 755.5 0 1211 189 14 755.5 0 14 190 13 755.5 0 13 191 16 755.5 0 16 192 6 755.5 0 6 193 8 755.5 0 8 194 10 755.5 0 10 195 7 755.5 0 7 196 3 755.5 0 3 197 5 755.5 0 5 198 13 755.5 0 13 199 8 755.5 0 8 200 11 755.5 0 11 201 8 755.5 0 8 202 11 755.5 0 11 203 24 755.5 0 24 204 64 755.5 0 64 205 54 755.5 0 54 206 26 755.5 0 26 207 10 755.5 0 10 208 14 755.5 0 14 209 30 755.5 0 30 210 17 755.5 0 17 211 18 755.5 0 18 212 25 755.5 0 25 213 27 755.5 0 27 214 26 755.5 0 26 215 23 755.5 0 23 216 37 755.5 0 37 217 225 755.5 0 225 218 83 755.5 0 83 219 523 755.5 0 523 220 5 755.5 0 5 221 11 755.5 0 11 222 78 755.5 0 78 223 13 755.5 0 13 224 7 755.5 0 7 225 14 755.5 0 14 226 29 755.5 0 29 227 40 755.5 0 40 228 31 755.5 0 31 229 17 755.5 0 17 230 14 755.5 0 14 231 8 755.5 0 8 232 12 755.5 0 12 233 7 755.5 0 7 234 10 755.5 0 10 235 7 755.5 0 7 236 19 755.5 0 19 237 20 755.5 0 20 238 8 755.5 0 8 239 3 755.5 0 3 240 7 755.5 0 7 241 6 755.5 0 6 242 18 755.5 0 18 243 9 755.5 0 9 244 15 755.5 0 15 245 26 755.5 0 26 246 17 755.5 0 17 247 13 755.5 0 13 248 22 755.5 0 22 249 5 755.5 0 5 250 18 755.5 0 18 251 20 755.5 0 20 252 206 755.5 0 206 253 40 755.5 0 40 254 30 755.5 0 30 255 37 755.5 0 37 256 21 755.5 0 21 257 21 755.5 0 21 258 21 755.5 0 21 259 9 755.5 0 9 260 6 755.5 0 6 261 26 755.5 0 26 262 79 755.5 0 79 263 28 755.5 0 28 264 48 755.5 0 48 265 21 755.5 0 21 266 22 755.5 0 22 267 32 755.5 0 32 268 27 755.5 0 27 269 5 755.5 0 5 270 6 755.5 0 6 271 10 755.5 0 10 272 10 755.5 0 10 273 14 755.5 0 14 274 21 755.5 0 21 275 13 755.5 0 13 276 17 755.5 0 17 277 16 755.5 0 16 278 32 755.5 0 32 279 32 755.5 0 32 280 80 755.5 0 80 281 25 755.5 0 25 282 22 755.5 0 22 283 12 755.5 0 12 284 12 755.5 0 12 285 34 755.5 0 34 286 31 755.5 0 31 287 26 755.5 0 26 288 10 755.5 0 10 289 15 755.5 0 15 290 19 755.5 0 19 291 11 755.5 0 11 292 15 755.5 0 15 293 6 755.5 0 6 294 15 755.5 0 15 295 15 755.5 0 15 296 29 755.5 0 29 297 75 755.5 0 75 298 34 755.5 0 34 299 17 755.5 0 17 300 31 755.5 0 31 301 10 755.5 0 10 Finished in 1.10 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 24,354 Reads with adapters: 8,493 (34.9%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 8,492 (34.9%) Total basepairs processed: 5,051,878 bp Total written (filtered): 2,326,379 bp (46.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8493 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 85 0.0 3 0 81 3 1 20 8367 0.0 4 8226 136 4 1 21 25 0.0 4 2 22 1 22 2 0.0 4 0 0 2 23 4 0.0 4 0 0 0 3 1 24 1 0.0 4 1 66 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 138 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 8,492 Reads with adapters: 132 (1.6%) Reads written (passing filters): 8,492 (100.0%) Total basepairs processed: 2,326,379 bp Total written (filtered): 2,321,675 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 132 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.6% C: 1.5% G: 86.4% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 33.2 0 1 11 117 0.0 1 3 114 12 1 0.0 1 0 1 261 12 0.0 1 12 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.